Article
Cell & Tissue Engineering
Young-Woo Jo, Inkuk Park, Kyusang Yoo, Hyun-Young Woo, Ye Lynne Kim, Yea-Eun Kim, Ji-Hoon Kim, Young-Yun Kong
Summary: This study found that Notch1 and Notch2 play distinct roles in maintaining the population of myogenic progenitors (MPs) during fetal myogenesis, with Notch1 inducing cell cycle exit and Notch2 suppressing premature differentiation. The activation of Notch1 and Notch2 signaling in MPs is also shown to be distinctively regulated by interactions with Notch ligand-expressing myofibers and MP-lineage cells, respectively.
Article
Cardiac & Cardiovascular Systems
Sanghamitra Sahoo, Yao Li, Daniel de Jesus, John Sembrat, Mauricio M. Rojas, Elena Goncharova, Eugenia Cifuentes-Pagano, Adam C. Straub, Patrick J. Pagano
Summary: The study reveals a crucial role for Notch2 in maintaining lung vascular endothelial cell quiescence and in PAH, with loss of Notch2 activating Notch1 and inducing proliferation and apoptosis resistance in human pulmonary artery endothelial cells. PAH patients exhibit reduced levels of endothelial Notch2 in pulmonary arteries, suggesting Notch2 as a fundamental driver of PAH pathogenesis.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Article
Oncology
Dawei Li, Dan Xu, Yifei Zhang, Penghui Chen, Jin Xie
Summary: The study demonstrates that Notch1 signaling plays a pivotal role in regulating the proliferation and apoptosis resistance of laryngeal cancer cells. Additionally, hypoxia may promote the proliferation and inhibit the apoptosis of these cells.
WORLD JOURNAL OF SURGICAL ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Manuela Minguzzi, Veronica Panichi, Stefania D'Adamo, Silvia Cetrullo, Luca Cattini, Flavio Flamigni, Erminia Mariani, Rosa Maria Borzi
Summary: Notch signaling pathway plays a critical role in cartilage development and homeostasis, with NOTCH1 being the key receptor that can affect the health status of osteoarthritis chondrocytes by regulating proliferation, hypertrophy, and terminal differentiation. Silencing NOTCH1 showed a healthier phenotype in OA chondrocytes, suggesting it is a convenient therapeutic target to attenuate OA progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Dawei Li, Dan Xu, Penghui Chen, Jin Xie
Summary: This study found that hypoxia can upregulate the expression and signaling activity of Notch1, and inhibiting Notch1 expression can decrease the expression of MDR and survivin genes in laryngeal cancer cells under hypoxic conditions. Additionally, suppressing Notch1 expression can increase the sensitivity of hypoxic laryngeal cancer cells to drugs, enhance drug-induced apoptosis, and increase intracellular drug accumulation.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Endocrinology & Metabolism
Woo Kyung Lee Doolittle, Li Zhao, Sheue-Yann Cheng
Summary: This study found that blocking the activity of cyclin-dependent protein 7 (CDK7) could impede the progression of anaplastic thyroid cancer (ATC) by inhibiting cancer stem cell (CSC) activity. The NOTCH1-cMYC signaling axis was identified as a potential target of CDK7 inhibition, and targeting this pathway may be a promising therapeutic strategy for ATC.
Article
Biochemistry & Molecular Biology
Ling-Ling Wang, Xiao-Yun Wan, Chun-Qi Liu, Fei-Meng Zheng
Summary: The study revealed a novel function of NDR1 in regulating BCSC properties by activating the Notch pathway, providing a potential strategy for eradicating BCSC to overcome tumor relapses, metastasis and drug resistance.
MOLECULAR MEDICINE
(2022)
Article
Multidisciplinary Sciences
Yuwen Zhao, Rui Yang, Zoe Bousraou, Kiarra Richardson, Shue Wang
Summary: This study established a LNA/DNA nanobiosensor for gene expression analysis in single hMSCs during osteogenic differentiation. The results showed that Notch1-Dll4 signaling plays an important role in regulating hMSCs osteogenic differentiation. Additionally, Dll4 was identified as a molecular signature of differentiated hMSCs during osteogenic induction, and the role of Notch1-Dll4 signaling in a 3D spheroid model was elucidated.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Matthew N. Creyer, Zhicheng Jin, Maurice Retout, Wonjun Yim, Jiajing Zhou, Jesse V. Jokerst
Summary: This study investigates the optical shifts and color changes of gold-silver core-shell nanoparticles, as well as their application in enzyme concentration detection.
Article
Multidisciplinary Sciences
Mohammad K. Manik, Yun Shi, Sulin Li, Mark A. Zaydman, Neha Damaraju, Samuel Eastman, Thomas G. Smith, Weixi Gu, Veronika Masic, Tamim Mosaiab, James S. Weagley, Steven J. Hancock, Eduardo Vasquez, Lauren Hartley-Tassell, Nestoras Kargios, Natsumi Maruta, Bryan Y. J. Lim, Hayden Burdett, Michael J. Landsberg, Mark A. Schembri, Ivan Prokes, Lijiang Song, Murray Grant, Aaron DiAntonio, Jeffrey D. Nanson, Ming Guo, Jeffrey Milbrandt, Thomas Ve, Bostjan Kobe
Summary: This study reveals the production process of cADPR isomers and identifies 3'cADPR as an antiviral agent in bacteria and an immune-suppressing signaling molecule in plants.
Article
Biochemistry & Molecular Biology
Yujing Ren, Beiming Yu, Lihui Zhou, Feng Wang, Yanfeng Wang
Summary: This study analyzed the phosphorylation mechanism of UCHL3 in its deubiquitinating activity and provided in vitro evidence of UCHL3's cleavage activity towards specific types of polyubiquitin chains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Belen Pacheco-Fiallos, Matthias K. Vorlaender, Daria Riabov-Bassat, Laura Fin, Francis J. O'Reilly, Farja I. Ayala, Ulla Schellhaas, Juri Rappsilber, Clemens Plaschka
Summary: Newly made mRNAs are recognized and organized by the essential TREX complex for nuclear export. Cryo-electron microscopy and tomography structures reveal that mRNAs are recognized through multivalent interactions between TREX subunit ALYREF and mRNP-bound exon junction complexes. Endogenous mRNPs form compact globules that are coated by multiple TREX complexes, providing a framework to understand mRNA biogenesis and export.
Article
Nutrition & Dietetics
Wanwan Zhuansun, Jun Xu, Hengzhao Liu, Ying Zhao, Lulu Chen, Shufang Shan, Shiqin Song, Haoyu Zhang, Tingting Dong, Huawei Zeng, Qinxiang Xu
Summary: The fermentation medium of a newly identified Cordyceps cicadae S1 was optimized, leading to the discovery of a novel selenium-enriched polysaccharide PACI-1 with strong antioxidant capacity.
FRONTIERS IN NUTRITION
(2022)
Article
Biochemistry & Molecular Biology
Anja C. Nagel, Dominik Mueller, Mirjam Zimmermann, Anette Preiss
Summary: The membrane-bound Notch regulator (Mnr) supports Notch signaling by promoting the cleavage of the Notch receptor, which in turn enhances Notch receptor activation. Overexpression of Mnr stimulates γ-secretase activity, while knockdown of Mnr inhibits this activity, demonstrating Mnr as a positive regulator of Notch signaling.
Article
Multidisciplinary Sciences
Jianjie Zhou, Yu Xiao, Yifei Ren, Jiwan Ge, Xinquan Wang
Summary: The TIR domains in the IL-1R family play a crucial role in signal transmission, and the structurally variant regions and key residues in the helices greatly impact the signaling process.
Article
Biology
Amanda N. Hayward, Eric J. Aird, Wendy R. Gordon
Article
Biochemical Research Methods
Alina C. Zdechlik, Yungui He, Eric J. Aird, Wendy R. Gordon, Daniel Schmidt
BIOCONJUGATE CHEMISTRY
(2020)
Correction
Multidisciplinary Sciences
Beau R. Webber, Cara-lin Lonetree, Mitchell G. Kluesner, Matthew J. Johnson, Emily J. Pomeroy, Miechaleen D. Diers, Walker S. Lahr, Garrett M. Draper, Nicholas J. Slipek, Branden A. Smeester, Klaus N. Lovendahl, Amber N. McElroy, Wendy R. Gordon, Mark J. Osborn, Branden S. Moriarity
NATURE COMMUNICATIONS
(2019)
Article
Chemistry, Multidisciplinary
Eric J. Aird, Kassidy J. Tompkins, Maria Paz Ramirez, Wendy R. Gordon
Article
Multidisciplinary Sciences
Bryan J. Jones, Robert L. Evans, Nathan J. Mylrea, Debayan Chaudhury, Christine Luo, Bo Guan, Colin T. Pierce, Wendy R. Gordon, Carrie M. Wilmot, Romas J. Kazlauskas
Article
Biochemistry & Molecular Biology
Kassidy J. Tompkins, Mo Houtti, Lauren A. Litzau, Eric J. Aird, Blake A. Everett, Andrew T. Nelson, Leland Pornschloegl, Lidia K. Limon-Swanson, Robert L. Evans, Karen Evans, Ke Shi, Hideki Aihara, Wendy R. Gordon
Summary: Replication initiator proteins from the HUH-endonuclease superfamily process specific ssDNA sequences to initiate replication in viruses. The team solved the first co-crystal structures of Reps complexed to ssDNA and developed a deep sequencing cleavage assay HUH-seq for analyzing Rep specificity.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Sunnie Kong, Shruti Kumta, Didem Vardar-Ulu
Summary: This study utilized instructional videos created by former students and integrated them into electronic lab notebooks to recreate a community-learning environment for students. This approach proved effective in a de-densified biochemistry laboratory setting.
BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION
(2022)
Article
Biochemistry & Molecular Biology
Maria Paz Ramirez, Sivaraman Rajaganapathy, Anthony R. Hagerty, Cailong Hua, Gloria C. Baxter, Joseph Vavra, Wendy R. Gordon, Joseph M. Muretta, Murti V. Salapaka, James M. Ervasti
Summary: Duchenne muscular dystrophy is a lethal muscle wasting disease caused by the absence of the protein dystrophin. Utrophin, a dystrophin homologue, is being investigated as a potential protein replacement therapy. This study shows that phosphorylation can improve the mechanical properties of utrophin, suggesting its potential efficacy in treating dystrophinopathies.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Microbiology
Adam T. Smiley, Kassidy J. Tompkins, Matthew R. Pawlak, August J. Krueger, Robert L. L. Evans III, Ke Shi, Hideki Aihara, Wendy R. Gordon
Summary: This study investigates the structural and functional dependencies of the Rep protein from Muscovy duck circovirus on ssDNA interaction. It reveals that divalent cations and substrate Watson-Crick base pairing play important roles in Rep-mediated DNA cleavage and rejoining, with a universal requirement for replication initiation in circular Rep-encoding single-stranded DNA viruses.
Article
Multidisciplinary Sciences
Matthew R. Pawlak, Adam T. Smiley, Maria Paz Ramirez, Marcus D. Kelly, Ghaidan A. Shamsan, Sarah M. Anderson, Branden A. Smeester, David A. Largaespada, David J. Odde, Wendy R. Gordon
Summary: Mechanical forces drive critical cellular processes, and the authors present Rupture And Deliver Tension Gauge Tethers (RAD-TGTs) which can record the mechanical history of individual cells by using flow cytometry or sequencing. RAD-TGTs recapitulate prior studies on DNA tension probes and can detect fluorescence gain in the force-generating cell. They can differentiate distinct mechanotypes and have the potential for various applications in combination with CRISPR screens and -omics analysis.
NATURE COMMUNICATIONS
(2023)
Article
Oncology
Kelly Birch, Sukyung Chung, Sean R. Zion, Joanna P. MacEwan, Michael J. Malecki
Summary: This study assessed the incremental costs of anxiety and depression among Medicare beneficiaries with cancer and found that one in three cancer patients had a diagnosis of anxiety or depression. Newly onset anxiety and depression were associated with an increase in healthcare costs of $17,496 per year. Screening and managing mental health conditions should be a part of coordinated oncology care for cancer patients.
JCO ONCOLOGY PRACTICE
(2023)
Article
Economics
Dingwei Dai, Henriette E. Coetzer, Sean R. Zion, Michael J. Malecki
Summary: Breast cancer patients often experience anxiety, depression, and stress. This study found that 38.2% of newly diagnosed patients were diagnosed with psychiatric disorders. These disorders were associated with higher healthcare resource utilization and costs.
JOURNAL OF HEALTH ECONOMICS AND OUTCOMES RESEARCH
(2023)
Article
Medicine, Research & Experimental
Mareike D. Hoffmann, Alina C. Zdechlik, Yungui He, David Nedrud, George Aslanidi, Wendy Gordon, Daniel Schmidt
Summary: This study discovered the high adaptability of AAV capsids to large domain insertions and identified engineerable hotspots associated with AAV assembly, stability, and infectivity.
MOLECULAR THERAPY METHODS & CLINICAL DEVELOPMENT
(2023)
Article
Oncology
Dingwei Dai, Henriette Coetzer, Sean R. R. Zion, Michael J. J. Malecki
Summary: This study aimed to examine the prevalence of multimorbidity and its associations with anxiety and depression among newly diagnosed patients with breast cancer. The results showed that multimorbidity was highly prevalent among patients with breast cancer and was strongly associated with an increased risk of anxiety and depression in the first year following diagnosis.
Article
Multidisciplinary Sciences
Maria Paz Ramirez, Michael J. M. Anderson, Marcus D. Kelly, Lauren J. Sundby, Anthony R. Hagerty, Sophia J. Wenthe, David J. Odde, James M. Ervasti, Wendy R. Gordon
Summary: The essential muscle protein dystrophin plays a role in maintaining cell membrane stability by connecting the actin cytoskeleton to the extracellular matrix through the dystrophin-glycoprotein complex. This study found that mutations associated with Duchenne or Becker muscular dystrophy compromise the mechanical function of dystrophin, leading to decreased focal adhesion tension and impaired cell migration. The results suggest that dystrophin influences focal adhesion tension and that disease-causing missense mutations may disrupt a cellular tension-sensing pathway in dystrophic skeletal muscle.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)