Article
Biochemistry & Molecular Biology
Wei Cai, Junxun Zhang, Hong Zhou, Xiangxiao Li, Fangzhou Lou, Yang Sun, Zhenyao Xu, Jing Bai, Qianqian Yin, Zhikai Wang, Libo Sun, Xiaojie Cai, Sibei Tang, Yue Wu, Li Fan, Hong Wang, Honglin Wang, Qun Li
Summary: Our study reveals that loss of Pp6 in T-reg cells leads to spontaneous autoinflammation and decreased FoxP3 levels, exacerbating T-reg-related autoinflammation in mice. Mechanistically, Pp6 deficiency affects DNA methylation and Akt signaling, resulting in impaired FoxP3 expression in T-reg cells.
Article
Biotechnology & Applied Microbiology
Huili Xia, Lingling Shangguan, Sheng Chen, Qiao Yang, Xiaoling Zhang, Lan Yao, Shihui Yang, Jun Dai, Xiong Chen
Summary: This study focuses on the biosynthesis of 2-phenylethanol (2-PE) using whole-cell bioconversion with Saccharomyces cerevisiae. It is found that the addition of rapamycin (Rap) can enhance the efficiency of 2-PE synthesis by increasing the flux of the Ehrlich pathway. The key role of GLN3 in the regulation of Rap is also identified. Commercial yeast is verified to be applicable for 2-PE production. The results demonstrate a new approach for the efficient synthesis of 2-PE.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Petra Vande Zande, Patricia J. Wittkopp
Summary: This study uses expression and fitness data from Saccharomyces cerevisiae gene deletion strains to show that cis-acting mutations tend to have lower pleiotropy and trans-acting mutations are more deleterious. These findings provide strong support for the role of pleiotropy in the preferential fixation of cis-regulatory alleles over evolutionary time.
MOLECULAR BIOLOGY AND EVOLUTION
(2022)
Review
Biochemistry & Molecular Biology
Csaba Mathe, Csongor Freytag, Adrienn Kelemen, Marta M-Hamvas, Tamas Garda
Summary: This review summarizes the multiple regulatory mechanisms of protein phosphatase PP2A, including regulation of metabolic pathways, subcellular localization, cellular processes, and stress responses in plants. The findings contribute to a better understanding of plant cell functioning, potential agricultural applications, and insights into how vascular plants including crops cope with diverse environmental challenges.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Micaela Boiero Sanders, Christopher P. Toret, Audrey Guillotin, Adrien Antkowiak, Thomas Vannier, Robert C. Robinson, Alphee Michelot
Summary: The use of different actin isoforms in eukaryotic cells and the molecular mechanisms of their segregation into distinct networks are poorly understood. By using yeast as a model, researchers found that the expression of heterologous actin causes significant reorganization of the actin cytoskeleton. However, the expression of two heterologous actin variants, each specialized in assembling a different network, can rescue cytoskeletal organization and increase resistance to external perturbation.
Article
Multidisciplinary Sciences
Wenting Shen, Ziqing Gao, Kaiyue Chen, Alusi Zhao, Qi Ouyang, Chunxiong Luo
Summary: Cells respond to environmental changes by modulating gene expression. In this study, the responses to osmotic stress in glucose-limited and glucose-rich environments were compared. It was found that cells exhibited a stronger osmotic gene expression response and larger integral of Hog1 nuclear localization under glucose-limited conditions. The regulatory mechanism of osmoresponse was derived and a reserve flux-dependent stress-defense strategy was proposed.
Article
Agriculture, Multidisciplinary
Ya-Ping Wang, Zhong-guan Sun, Xiao-Qing Wei, Xue-Wu Guo, Dong-Guang Xiao
Summary: This study identified key genes and pathways for the production of n-propanol by S. cerevisiae by deleting n-propanol metabolism-related genes. It revealed that glycine and threonine transformation and the formation of propanal from 2-ketobutyrate were core pathways for n-propanol synthesis, with YML082W playing an important role in directly producing 2-ketobutyric acid. The findings provide valuable insights for future optimization of yeast strains in Baijiu making.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Cell Biology
Ladislav Dokladal, Michael Stumpe, Zehan Hu, Malika Jaquenoud, Jorn Dengjel, Claudio De Virgilio
Summary: Eukaryotic TORC1 kinase controls growth by regulating anabolic and catabolic processes based on environmental cues. In yeast, TORC1 activates protein synthesis through Sch9 in response to abundant nutrients, while inhibition of TORC1 under nutrient limitation unlocks distally controlled kinases for coordinating the quiescence program.
Article
Biochemistry & Molecular Biology
Ana I. Garrido-Godino, Abel Cuevas-Bermudez, Francisco Gutierrez-Santiago, Maria del Carmen Mota-Trujillo, Francisco Navarro
Summary: Rtr1 plays a critical role in RNA pol II assembly and mRNA decay regulation by mediating the association between RNA pol II and Rpb4/7. This study reveals new mechanisms in gene regulation and the crosstalk between mRNA synthesis and decay.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Ching-Hua Shih, Justin Fay
Summary: The evolution of cis-regulatory sequences is influenced by their impact on gene expression. Studies have shown that allele-specific expression dynamics are affected by cis-acting variations within and between species, suggesting a key role of cis-regulatory sequences in tuning gene expression dynamics.
Article
Multidisciplinary Sciences
Jan Zrimec, Xiaozhi Fu, Azam Sheikh Muhammad, Christos Skrekas, Vykintas Jauniskis, Nora K. Speicher, Christoph S. Boerlin, Vilhelm Verendel, Morteza Haghir Chehreghani, Devdatt Dubhashi, Verena Siewers, Florian David, Jens Nielsen, Aleksej Zelezniak
Summary: Designing de novo synthetic regulatory DNA is a promising approach for controlling gene expression in biotechnology and medicine. In this study, the researchers developed ExpressionGAN, a generative adversarial network that uses genomic and transcriptomic data to generate regulatory DNA sequences with predefined target mRNA levels.
NATURE COMMUNICATIONS
(2022)
Article
Biotechnology & Applied Microbiology
Bo Li, Li Wang, Jin-Yu Xie, Zi-Yuan Xia, Cai-Yun Xie, Yue-Qin Tang
Summary: This study revealed the regulatory mechanisms of Haa1p and Tye7p in improving the fermentation performance and inhibitor tolerance of S. cerevisiae through comparative transcriptomic analyses.
MICROBIAL CELL FACTORIES
(2022)
Article
Cell Biology
Diana Ruethnick, Jlenia Vitale, Annett Neuner, Elmar Schiebel
Summary: This study reveals the initial steps of SPB assembly in yeast, including the interaction between N-Sfi1 and SPB components, as well as a new function of Cdc31 in recruiting dSPB components. These findings provide important insights into our understanding of SPB assembly.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Weiquan Zhao, Xinning Wang, Bolun Yang, Ying Wang, Zailu Li, Xiaoming Bao
Summary: Improving the resistance of Saccharomyces cerevisiae to vanillin, derived from lignin, is important for the design of robust cell factories for lignocellulosic biorefining. The transcription factor Yrr1p plays a role in S. cerevisiae resistance to various compounds. In this study, certain mutated forms of Yrr1p were found to enhance vanillin resistance. Dephosphorylated mutants of Yrr1p promoted target gene expression, while phosphorylated mutants inhibited it. The identification of key phosphorylation sites in Yrr1p provides new targets for constructing mutants that improve resistance to other compounds.
MICROBIAL CELL FACTORIES
(2023)
Article
Microbiology
Marcel Albacar, Lenka Sacka, Carlos Calafi, Diego Velazquez, Antonio Casamayor, Joaquin Arino, Olga Zimmermannova
Summary: Overexpression of Ppz1 disrupts the fine balance of phospho/dephosphorylation reactions in the cell, leading to cell proliferation blockage and changes in transcriptomic and phosphoproteomic profiles. The toxic effect is related to the overactivation of Nha1, resulting in excessive entry of H+ and efflux of K+, which is detrimental for cell growth. The deletion of NHA1 gene counteracts the growth defect caused by Ppz1 overexpression.