Article
Multidisciplinary Sciences
Yi Na Dong, Fu-Chun Hsu, Cynthia J. Koziol-White, Victoria Stepanova, Joseph Jude, Andrei Gritsiuta, Ryan Rue, Rosalind Mott, Douglas A. Coulter, Reynold A. Panettieri, Vera P. Krymskaya, Hajime Takano, Elena A. Goncharova, Dmitry A. Goncharov, Douglas B. Cines, David R. Lynch
Summary: The study indicates the expression of functional NMDA receptors in human pulmonary artery smooth muscle cells, suggesting a role in pulmonary vasoconstriction. This finding offers a new therapeutic target for pharmacological modulation in conditions associated with pulmonary vascular dysfunction.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Sofie Albinsson, Christine Lingblom, Christina Lundqvist, Viktoria Hennings, Esbjorn Telemo, Olov Ekwall, Christine Wenneras
Summary: Differentiation of eosinophil precursors occurs in the human thymus, where they are located in the corticomedullary junction. This study aimed to investigate the distinct thymic eosinophil populations and their role in the development of thymocytes. Comparison of thymic and blood eosinophils revealed several specific populations in both tissues. Co-culture experiments showed that thymic eosinophils increase the number of CD4 single-positive thymocytes and decrease the frequency of CD8 single-positive thymocytes, promoting a more mature thymic phenotype. Thymic eosinophils exhibit a unique phenotype and preferentially support the development of CD4 thymocytes.
Article
Immunology
Shuangpeng Kang, Qiongli Wu, Jun Huang, Binyan Yang, Changyan Liang, Peidong Chi, Changyou Wu
Summary: This study found that γδT cells in the uterus are mainly distributed in the endometrium, with memory cell characteristics and high expression of IL-17 upon activation, promoting the invasion of murine trophocytes. These findings provide a foundation for future research on the role of uterine γδT cells in pregnancy and autoimmune diseases.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Albert C. Sek, Caroline M. Percopo, Arun K. Boddapati, Michelle Ma, Wendy E. Geslewitz, Julia O. Krumholz, Justin B. Lack, Helene F. Rosenberg
Summary: Eosinophils are no longer considered simply as cytotoxic effectors, but as complex cells with unique phenotypes that respond to stimuli in the local microenvironment. RNA sequencing revealed up-regulation of tissue remodeling genes in eosinophils from dystrophin-deficient mdx mice, and effector proteins in eosinophils from IL5tg mice without muscle lesions. Additionally, the immunomodulatory receptor Trem2 was found to be critical for eosinophil growth and differentiation, with expression correlating with donor characteristics in human peripheral blood eosinophils.
JOURNAL OF LEUKOCYTE BIOLOGY
(2021)
Article
Immunology
Jan-Hendrik Schroeder, Gordon Beattie, Jonathan W. W. Lo, Tomasz Zabinski, Nick Powell, Joana F. F. Neves, Richard G. G. Jenner, Graham M. M. Lord
Summary: Significant progress has been made in understanding innate lymphoid cells (ILC) by applying concepts from T cell biology, such as flow cytometry gating strategies and CD90 markers. Surprisingly, a sub-population of ILC in the gut were found to have low or no expression of CD90, contrary to expectations. These CD90-negative and CD90-low CD127(+) ILC were influenced by stimulatory cues and dysbiosis, and could produce IL-13, IFN gamma and IL-17A in steady state and colitis conditions.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Genevieve T. Clutton, Ann Marie K. Weideman, Melissa A. Mischell, Sallay Kallon, Shayla Z. Conrad, Fiona R. Shaw, Joanna A. Warren, Lin Lin, JoAnn D. Kuruc, Yinyan Xu, Cynthia M. Gay, Paul M. Armistead, Michael G. Hudgens, Nilu P. Goonetilleke
Summary: CD8 T cells recognize infected and cancerous cells through their T-cell receptor (TCR) and the affinity between TCR and peptide-MHC complexes contributes to antigen sensitivity. CD3 and CD8 downmodulation are strongly correlated with functional avidity in CD8 T cells. A simple flow cytometry assay can be used to identify high-avidity CD8 T cells.
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Adi Egozi, Dhana Llivichuzhca-Loja, Blake T. McCourt, Keren Bahar Halpern, Lydia Farack, Xiaojing An, Fujing Wang, Kong Chen, Liza Konnikova, Shalev Itzkovitz
Summary: Analysis of single-cell transcriptomics in human fetal and neonatal intestinal cells has revealed a subpopulation of enteroendocrine cells that produce insulin during development. This finding suggests a potential extra-pancreatic source of beta cells, providing a new avenue for diabetes treatment.
Article
Allergy
Dong Hyun Kim, Ji Youn Lim, Jung Yeon Jang, Jangwook Gwak, Hye Ah Joo, Sungseok Ryu, Ji Heui Kim
Summary: This study aimed to analyze the role of group 2 innate lymphoid cells (ILC2s) in eosinophilic chronic rhinosinusitis with nasal polyps (CRSwNPs) and evaluate the differentiation of ILCs. The results showed that ILC1s were associated with neutrophils, while ILC2s were associated with eosinophils. In addition, ILC precursor cells (ILCPs) in the sinus mucosa could differentiate into cells producing IFN-y or IL-5 in response to local cytokine stimuli.
ALLERGOLOGY INTERNATIONAL
(2023)
Article
Multidisciplinary Sciences
Debora Tibbe, Yingzhou Edward Pan, Carsten Reissner, Frederike L. Harms, Hans-Juergen Kreienkamp
Summary: CASK, a member of the MAGUK family, serves various cellular functions depending on interactions with different partners, while alternative splicing affects the affinities between CASK isoforms and interaction partners.
Article
Multidisciplinary Sciences
Qin Gong, Kim Robinson, Chenrui Xu, Phuong Thao Huynh, Kelvin Han Chung Chong, Eddie Yong Jun Tan, Jiawen Zhang, Zhao Zhi Boo, Daniel Eng Thiam Teo, Kenneth Lay, Yaming Zhang, John Soon Yew Lim, Wah Ing Goh, Graham Wright, Franklin L. Zhong, Bruno Reversade, Bin Wu
Summary: NLRP1 and CARD8 are two recently characterized sensor proteins for the human inflammasome complex. The cryo-EM CARD filament structures of the NLRP1 and CARD8 activating domains reveal how NLRP1 and CARD8 discriminate between ASC and pro-caspase-1. The authors propose a two-step model for NLRP1 activation.
NATURE COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Patrick Mayrhofer, Monika Hunjadi, Renate Kunert
Summary: The spike protein of SARS-CoV-2 is essential for infection, and providing high-quality recombinant spike protein is crucial for studying virus-cell interactions. While most studies use truncated versions like the receptor-binding domain, additional mutant variants offer new insights into virus assembly and infection mechanisms.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Cell Biology
Yuan Song, Huey Yee Teo, Yonghao Liu, Xiaomeng Zhang, Jinmiao Chen, Yongliang Zhang, Haiyan Liu
Summary: This study found that IL-12/18 combination could significantly promote IFN-gamma secretion and cytotoxicity in human gamma delta T cells, but it also led to increased apoptosis and elevated expressions of co-inhibitory receptors. Further research demonstrated that IL-12/18-induced apoptosis of human gamma delta T cells was mediated by the activation of JNK pathway. Treatment with p-JNK inhibitor effectively revived human gamma delta T cells from the apoptosis induced by IL-12/18 and maintained their enhanced IFN-gamma production and cytotoxicity against tumor cells.
JOURNAL OF LEUKOCYTE BIOLOGY
(2022)
Article
Physiology
Nicolas Montalbetti, Aneta J. Przepiorski, Shujie Shi, Shaohu Sheng, Catherine J. Baty, Joseph C. Maggiore, Marcelo D. Carattino, Thitinee Vanichapol, Alan J. Davidson, Neil A. Hukriede, Thomas R. Kleyman
Summary: This study examines the functional expression of key ion channels in kidney organoids derived from pluripotent stem cells. It demonstrates the presence of large-conductance Ca2+-activated K+ (BKCa) channels, renal outer medullary K+ (ROMK, Kir1.1) channels, and epithelial Na+ channels (ENaC) in different cell populations within the organoids. These findings suggest that human kidney organoids can functionally produce important distal nephron K+ and Na+ channels.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Eric B. Nonnecke, Patricia A. Castillo, Malin E. Johansson, Edward J. Hollox, Bo Shen, Bo Lonnerdal, Charles L. Bevins
Summary: This study investigates the differences between ITLN2 and ITLN1 in terms of their structure and expression characteristics. It reveals that ITLN2 is a highly abundant Paneth cell-specific product in the small intestine, and its expression is downregulated in Crohn's disease. ITLN2 mRNA expression is undetectable in control colon tissue, but metaplastic Paneth cells express ITLN2 in both ulcerative colitis and colonic Crohn's disease.
Article
Biochemistry & Molecular Biology
Adam Mischler, Victoria Karakis, Jessica Mahinthakumar, Celeste K. Carberry, Adriana San Miguel, Julia E. Rager, Rebecca C. Fry, Balaji M. Rao
Summary: The trophectoderm layer of the blastocyst-stage embryo is the precursor for all trophoblast cells in the placenta. Human trophoblast stem (TS) cells have emerged as an attractive tool for studies on early trophoblast development. The derivation of TS cells from pluripotent stem cells will significantly enable construction of in vitro models for normal and pathological placental development.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Hematology
Cedric Rossi, Marie Tosolini, Pauline Gravelle, Sarah Pericart, Salim Kanoun, Solene Evrard, Julia Gilhodes, Don-Marc Franchini, Nadia Amara, Charlotte Syrykh, Pierre Bories, Lucie Oberic, Loic Ysebaert, Laurent Martin, Selim Ramla, Philippine Robert, Claire Tabouret-Viaud, Rene-Olivier Casasnovas, Jean-Jacques Fournie, Christine Bezombes, Camille Laurent
Summary: This study identified that baseline whole-body maximum standardized uptake (SUVmax) greater than 14.5 is associated with poorer progression-free survival (PFS) in follicular lymphoma (FL) patients. Immune T-cell infiltration and immune checkpoint expression were not associated with baseline PET metrics. However, FL samples with Ki-67 staining greater than or equal to 10% showed enrichment of cell cycle/DNA genes and significantly higher SUVmax values. High baseline SUVmax reflects FL tumor proliferation and can be used, along with Ki-67 proliferative index, to identify patients at risk of early relapse with rituximab chemotherapy.
Article
Allergy
Christoph Bergmann, Aurelie Poli, Ioana Agache, Rodolfo Bianchini, Heather J. Bax, Mariana Castells, Silvia Crescioli, David Dombrowicz, Denisa Ferastraoaru, Edda Fiebiger, Hannah J. Gould, Karin Hartmann, Elena Izquierdo, Galateja Jordakieva, Debra H. Josephs, Marek Jutel, Francesca Levi-Schaffer, Leticia de las Vecillas, Michael T. Lotze, Gabriel Osborn, Mariona Pascal, Frank Redegeld, David Rosenstreich, Franziska Roth-Walter, Carsten Schmidt-Weber, Mohamed Shamji, Esther H. Steveling, Michelle C. Turner, Eva Untersmayr, Erika Jensen-Jarolim, Sophia N. Karagiannis
Summary: This paper discusses the interactions between the immune system and several 'danger' signals, such as DAMPs, PAMPs, and AAMPs, and their roles in allergy and cancer. It focuses on their interactions with different immune cells and their contrasting clinical outcomes in allergy and cancer.
Editorial Material
Allergy
Laurent L'homme, David Dombrowicz
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Article
Oncology
Marcin Domagala, Loic Ysebaert, Laetitia Ligat, Frederic Lopez, Jean-Jacques Fournie, Camille Laurent, Mary Poupot
Summary: In chronic lymphocytic leukemia, M2-like nurse-like cells (NLC) provide protection against apoptosis for leukemic cells through the release of IL-10, while M1-like NLC do not have this effect. The balance between IL-10 and TNF plays an important role in maintaining the protective phenotype of NLCs and the survival of CLL cells.
Review
Endocrinology & Metabolism
Joanne A. Hoogerland, Bart Staels, David Dombrowicz
Summary: The incidence of non-alcoholic fatty liver disease (NAFLD) has significantly increased over the past two decades. NAFLD can progress to fibrosis and hepatocellular carcinoma (HCC) and the role of the immune system in hepatic physiology and NAFLD progression is increasingly recognized.
TRENDS IN ENDOCRINOLOGY AND METABOLISM
(2022)
Editorial Material
Hematology
Adeline Bertola, David Dombrowicz, Stoyan Ivanov
Article
Endocrinology & Metabolism
Arianna Marino, Laura Butruille, Denis A. Mogilenko, Arielle C. Nzoussi Loubota, Oscar Chavez-Talavera, Emilie Dorchies, Emmanuelle Vallez, Joel Haas, Bruno Derudas, Antonino Bongiovanni, Meryem Tardivel, Folkert Kuipers, Philippe Lefebvre, Sophie Lestavel, Anne Tailleux, David Dombrowicz, Sandrine Caron, Bart Staels
Summary: This study reveals the regulatory role of FXR in adipose tissue function in obese rats, improving glucose metabolism and systemic oxidative stress by inhibiting inflammatory responses and enhancing insulin sensitivity. Transcriptomic analysis identified the Gsta4 gene as the most upregulated gene in Ad-FXR-/- mouse adipocytes.
MOLECULAR METABOLISM
(2023)
Article
Endocrinology & Metabolism
Francesco Paolo Zummo, Alexandre Berthier, Celine Gheeraert, Manjula Vinod, Marie Bobowski-Gerard, Olivier Molendi-Coste, Laurent Pineau, Matthieu Jung, Loic Guille, Julie Dubois-Chevalier, David Dombrowicz, Bart Staels, Jerome Eeckhoute, Philippe Lefebvre
Summary: The liver plays a crucial role in maintaining organismal homeostasis through the function of nuclear receptors (NRs), which are transcription factors sensitive to external signals and regulated by intracellular signaling pathways. However, the understanding of NR functions in different liver cell populations is still limited. This study characterized the expression levels of NRs in the entire liver of mice under different time and diet conditions, and identified their cyclic expression patterns and cell-type specificity. Single-cell RNA sequencing analysis further revealed a zonation pattern of NRs in hepatocytes, liver sinusoidal cells, and stellate cells, highlighting the link between NR localization and liver functional specialization.
JOURNAL OF MOLECULAR ENDOCRINOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Sandro Ninni, David Dombrowicz, Tanya Kuznetsova, Rocio Vicario, Vance Gao, Olivier Molendi-Coste, Joel Haas, Eloise Woitrain, Augustin Coisne, Annette E. Neele, Koen Prange, Lisa Willemsen, Samy Aghezzaf, Stamatina Fragkogianni, Amine Tazibet, Laurent Pineau, James Robert White, Jerome Eeckhoute, Mohamed Koussa, Henri Dubrulle, Francis Juthier, Jerome Soquet, Andre Vincentelli, Jean-Louis Edme, Menno de Winther, Frederic Geissmann, Bart Staels, David Montaigne
Summary: This study aimed to evaluate the potential impact of hematopoietic somatic mosaicism (HSM) on preoperative blood and myocardial myeloid cells and explore its effect on postoperative outcomes. The results showed a significant association between HSM and the risk of atrial fibrillation, as well as an exaggerated inflammatory response following surgery. Therefore, assessing HSM may be beneficial in the personalized management of patients in the perioperative period.
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Taha Messelmani, Anne Le Goff, Fabrice Soncin, Francoise Gilard, Zied Souguir, Nathalie Maubon, Bertrand Gakiere, Cecile Legallais, Eric Leclerc, Rachid Jellali
Summary: Organ-on-chip technology is a promising in vitro approach for studying responses to drug exposure. This study used a metabolomic investigation to analyze a coculture of liver sinusoidal endothelial cells (LSECs) with hepatocytes using advanced organ-on-chip technology. The results demonstrated the potential of a metabolomic-on-chip strategy for pharmaco-metabolomic applications.
Article
Virology
Nathalie Garnier, Famara Sane, Layal Massara, Fabrice Soncin, Philippe Gosset, Didier Hober, Sabine Szunerits, Ilka Engelmann
Summary: miRNAs, small non-coding RNAs, play a role in regulating gene expression and may serve as potential biomarkers for predicting outcomes in severe COVID-19. This study aims to investigate the impact of SARS-CoV-2 infection on the expression of genes involved in miRNA biogenesis.
Article
Cell Biology
Nicolas Curdy, Olivia Lanvin, Juan-Pablo Cerapio, Frederic Pont, Marie Tosolini, Emeline Sarot, Carine Valle, Nathalie Saint-Laurent, Emeline Lhuillier, Camille Laurent, Jean-Jacques Fournie, Don-Marc Franchini
Summary: Stress granules (SGs) and processing bodies (PBs) are cytoplasmic assemblies that regulate mRNAs under environmental stress. This study characterizes the SGs and PBs in human T lymphocytes before and after stimulation using proteomic, transcriptomic, and immunofluorescence approaches. The findings reveal unexpected molecular and functional complementarity between SGs and PBs, although they maintain distinct spatial organizations and capacities to interact with mRNAs. This comprehensive characterization provides a valuable resource for future investigations on SGs and PBs in T lymphocytes.
Article
Nutrition & Dietetics
Lea Chantal Tran, Lucie Marousez, Marie De Lamballerie, Scott McCulloch, Emmanuel Hermann, Frederic Gottrand, Delphine Ley, Jean Lesage
Summary: The composition of the milk metabolome can be affected by different sterilization methods. High pressure sterilization has a greater impact on lipid compounds compared to pasteurization.
FRONTIERS IN NUTRITION
(2023)
Article
Microbiology
Amilcar Flores, Cristina Alonso-Vega, Emmanuel Hermann, Mary-Cruz Torrico, Nair Alaide Montano Villarroel, Faustino Torrico, Yves Carlier, Carine Truyens
Summary: Monocytes from newborns born to Trypanosoma cruzi infected mothers are more activated and capable of controlling T. cruzi infection better than monocytes from newborns born to uninfected mothers.
Article
Biology
Erwan Goy, Nathalie Martin, Claire Drullion, Laure Saas, Olivier Molendi-Coste, Laurent Pineau, David Dombrowicz, Emeric Deruy, Helene Bauderlique-Le-Roy, Olivier Samyn, Joe Nassour, Yvan de Launoit, Corinne Abbadie
Summary: Cellular senescence is a programmed cell state that is triggered as a response to various stresses, particularly those affecting genome integrity. Senescent cells accumulate in tissues with age and contribute to the development of several pathologies. The article describes a flow cytometry strategy for sorting senescent cells based on three senescence markers.