Article
Multidisciplinary Sciences
Yang Han, Lin Zhong, Fuli Ren
Summary: This study established a simple and broad-spectrum system for preparing phosphorylation-positive samples, which were then validated in cells from different species and tissues, demonstrating effectiveness in evaluating the quality of phosphorylation-specific antibodies.
Article
Multidisciplinary Sciences
Yang Han, Lin Zhong, Fuli Ren
Summary: This study established a simple and broad-spectrum system for evaluating the quality of phosphorylation-specific antibodies, which was validated in multiple experiments and shown to be effective and applicable in various biomedical fields.
Article
Multidisciplinary Sciences
Dhanashree Lokesh, P. S. N. Suresh, Rajagopal Kammara
Summary: This article describes the signal transduction pathway of prokaryotes, involving a peptidoglycan synthesis cluster and protein kinases. The authors developed a data repository of probiotic eSTKs, containing 830 STK sequences. This resource can be useful for sequence-based studies and drug development.
Article
Microbiology
Christian Seeger, Karl Dyrhage, Mayank Mahajan, Anna Odelgard, Sara Bergstrom Lind, Siv G. E. Andersson
Summary: Planctomycetes bacteria exhibit unique cell architectures with heavily invaginated membranes, as shown by three-dimensional models reconstructed from FIB-SEM images. The subcellular proteome analysis revealed notable differences compared to Escherichia coli, including unique sets of proteins and expanded protein families that may contribute to the evolution of cellular complexity.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Zixuan Wang, Chao Zhu, Youduo Wu, Wei Kang, Chaojun Wang, Ying Zhang, Chuang Xue
Summary: In this study, we identified and investigated six STK candidates in Clostridium beijerinckii. We found that the deletion of two STKs improved ABE production, while the deletion of other STKs significantly decreased sporulation frequencies. Additionally, overexpressing cbei0566 and cbei1929 demonstrated remarkable regulatory functions.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jui-Hung H. Weng, Phillip T. Aoto, Robin Lorenz, Jian Wu, Sven Schmidt, Jascha Manschwetus, Pallavi Kaila-Sharma, Steve W. Silletti, Sebastian S. Mathea, Deep Chatterjee, Stefan Knapp, Friedrich Herberg, Susan Taylor
Summary: This study generated a comprehensive dynamic allosteric portrait of the C-terminal domains of LRRK2 using HDX-MS and MD simulations. It identified two helices that regulate LRRK2 conformation and function, providing potential sites for pharmacological intervention.
Article
Chemistry, Medicinal
Yu Han, Huimin Zhang, Shuxiang Wang, Bo Li, Kun Xing, Yuntao Shi, Hongxue Cao, Jian Zhang, Tong Tong, Jie Zang, Lihong Guan, Xiaoxiao Gao, Yuetong Wang, Dan Liu, Min Huang, Yongkui Jing, Linxiang Zhao
Summary: A novel compound 21o with selective inhibition of MNKs and PIMs was developed, inhibiting the growth of leukemia cells without causing evident toxicity.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Vadim M. Gumerov, Ekaterina P. Andrianova, Miguel A. Matilla, Karen M. Page, Elizabet Monteagudo-Cascales, Annette C. Dolphin, Tino Krell, Igor B. Zhulin
Summary: This study identifies a common amino acid recognition mechanism in bacteria, archaea, and humans, which may lead to improvements in drug targeting pain and neurobiological disorders.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Mahmoud I. Khalil, Heba M. Ismail, Ganna Panasyuk, Anna Bdzhola, Valeriy Filonenko, Ivan Gout, Olivier E. Pardo
Summary: Ribosomal S6 kinases (S6Ks) play crucial roles in cell growth, homeostasis, and survival, and dysregulation of these kinases is implicated in various malignancies. While S6K1 has been extensively studied, the involvement of S6K2 in cancer progression has been overlooked. This study reveals that p54-S6K2 undergoes asymmetric dimethylation at Arg-475 and Arg-477, mediated by methyltransferases PRMT1, PRMT3, and PRMT6. This methylation event leads to nuclear localization of S6K2, which is crucial for its pro-survival effects during starvation-induced cell death. These findings highlight a novel post-translational modification that regulates p54-S6K2 function, and its relevance in cancer progression where general arginine methylation is often elevated.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Naresh Kumar, Claudina Perez-Novo, Priyanka Shaw, Emilie Logie, Angela Privat-Maldonado, Sylvia Dewilde, Evelien Smits, Wim Vanden Berghe, Annemie Bogaerts
Summary: Despite modern therapeutic advances, the survival prospects for pancreatic cancer patients remain poor. This study demonstrates that plasma treated water-derived oxidants sensitize pancreatic cancer cells to ferroptotic cell death by targeting a NRF2-HMOX1-GPX4 specific kinase signaling network. The novel kinome activity mapping approach identified vulnerable kinase dependencies for therapy sensitization by physical plasma.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
A. A. Zorina, G. V. Novikova, N. B. Gusev, A. V. Leusenko, D. A. Los, O. I. Klychnikov
Summary: Twelve potential serine-threonine protein kinases (STPKs) in Synechocystis sp. PCC 6803 genome were divided into two clusters: PKN2-type kinases and ABC1-type kinases. This study demonstrated that SpkH of Synechocystis is a true active serine protein kinase with substrate specificity similar to casein kinases and sensitivity to certain activity effectors.
Article
Oncology
Fizzah Abid, Talha Iqbal, Khushbukhat Khan, Yasmin Badshah, Janeen H. Trembley, Naeem Mahmood Ashraf, Maria Shabbir, Tayyaba Afsar, Ali Almajwal, Suhail Razak
Summary: This study identified an association between PKC gamma SNP rs1331262028 and HCV-induced hepatocellular carcinoma. The SNP was found to decrease protein stability and induce HCC. Genotype analysis revealed a higher correlation between the homozygous wild type AA and the disease, while patients with genotype AG had higher viral load.
BIOMARKER RESEARCH
(2022)
Article
Multidisciplinary Sciences
Andrea Piserchio, Kimberly J. Long, Luke S. Browning, Amanda L. Bohanon, Eta A. Isiorho, Kevin N. Dalby, Ranajeet Ghose
Summary: Protein translation, a high-energy process, requires robust regulation under energy-deprived conditions. The eEF-2K plays a crucial role in this regulation by suppressing translational elongation through phosphorylation of eEF-2. It has been suggested that eEF-2K's response to energy levels is mediated by AMPK and the mTOR pathway.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Zijun Wang, Brian K. Kennedy, Lik-Wei Wong, Sreedharan Sajikumar
Summary: Age-induced impairments in learning and memory are caused by changes in hippocampal synaptic plasticity. The p75 neurotrophin receptor (p75(NTR)) and mechanistic target of rapamycin (mTOR) are involved in synaptic plasticity processes. The interaction between p75(NTR) and mTOR mediates age-induced impairment of hippocampal synaptic plasticity. However, the specific effects of this interaction and the role of mTOR in age-induced cognitive decline are still unclear.
Article
Andrology
Lian-Zhong Wu, Yi-Qin Weng, Yi-Xin Ling, Shu-Juan Zhou, Xiao-Kai Ding, Si-Qi Wu, Kang Yu, Song-Fu Jiang, Yi Chen
Summary: Through scientometric analysis, researchers have explored the research progress and future trends of the mTOR pathway in kidney disease. The study identified diabetic nephropathy, kidney transplantation, autosomal dominant polycystic kidney disease, tuberous sclerosis complex, renal cell carcinoma and autophagy as the future research hot spots.
TRANSLATIONAL ANDROLOGY AND UROLOGY
(2021)