Article
Microbiology
Dana Duncan, John Duncan, Bastian Kramer, Alex Y. Nilsson, Betiel Haile, Ann Butcher, Shikha Chugh, Paul Baum, Grace M. Aldrovandi, Stephen Young, Ann K. Avery, Karen Tashima, Alexandra Valsamakis, Joseph D. Yao, Ming Chang, Robert W. Coombs
Summary: The diagnostic testing algorithms for HIV-1 and HIV-2 involve up to three tests and rely on detection of viral antigen and host antibody responses. Alternative algorithms using HIV-1/HIV-2 qualitative NAT show promising results in discriminating between the two types of HIV.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Virology
Bonolo Rankotsane Mashishi, Zinhle Makatini, Clement Gascua Adu-Gyamfi
Summary: HIV-1/2 testing is crucial for diagnosing and managing HIV-infected individuals. Suboptimal testing algorithms can lead to increased rates of misdiagnosis of HIV infection. The World Health Organization recommends high burden countries to revise their testing algorithm from a 2 to 3-test strategy, with the new algorithm needing to balance operational feasibility, patient outcomes, and cost-effectiveness.
JOURNAL OF CLINICAL VIROLOGY
(2021)
Article
Biochemical Research Methods
Ian Dardani, Benjamin L. Emert, Yogesh Goyal, Connie L. Jiang, Amanpreet Kaur, Jasmine Lee, Sara H. Rouhanifard, Gretchen M. Alicea, Mitchell E. Fane, Min Xiao, Meenhard Herlyn, Ashani T. Weeraratna, Arjun Raj
Summary: clampFISH 2.0 is an efficient method for detecting multiple RNA species and amplifying signals simultaneously, enabling the detection of 10 different RNA species in over 1 million cells and also applicable to tissue sections.
Review
Infectious Diseases
Basilua Andre Muzembo, Kei Kitahara, Anusuya Debnath, Keinosuke Okamoto, Shin-Ichi Miyoshi
Summary: This study systematically evaluated the accuracy of cholera rapid diagnostic tests (RDTs) and found that they have moderate sensitivity and specificity, especially when enriched with alkaline peptone water (APW). Although they are less useful for clinical management, the current generation of RDTs can be valuable for surveillance efforts if used in a principled manner.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Review
Immunology
Bridget Mugisa, Ahmed Sabry, Yvan Hutin, Joumana Hermez
Summary: This study reviewed the HIV epidemic in the WHO Eastern Mediterranean region and found that inefficient testing and suboptimal treatment have led to underdiagnosis, ongoing transmission, delayed treatment, and increased mortality. To accelerate the response, a change in thinking is needed to target high-risk populations for testing, transition to optimized treatment, and provide patient-centered services.
Article
Immunology
Beatrice N. Vetter, Elena Ivanova Reipold, Stefano Ongarello, Rosemary Audu, Fehintola A. Ige, Maia Alkhazashvili, Nazibrola Chitadze, Fien Vanroye, Anja De Weggheleire, Sokkab An, Katrien Fransen
Summary: This study evaluated the performance of 13 rapid diagnostic tests (RDTs) for detecting hepatitis C virus antibodies. The sensitivity and specificity were high in HIV-uninfected plasma samples, but sensitivity was markedly reduced in HIV-infected samples.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Review
Public, Environmental & Occupational Health
Basilua Andre Muzembo, Kei Kitahara, Ayumu Ohno, Ngangu Patrick Ntontolo, Nlandu Roger Ngatu, Keinosuke Okamoto, Shin-Ichi Miyoshi
Summary: The pooled sensitivity and specificity of rapid diagnostic tests for Ebola virus were found to be 86% and 95% respectively, with sensitivity decreasing to 83% after removing outliers. When studies using RT-PCR from altona Diagnostics as the gold standard were analyzed, pooled sensitivity increased to 90%.
BULLETIN OF THE WORLD HEALTH ORGANIZATION
(2021)
Article
Plant Sciences
Tammy Stackhouse, Sarah L. Boggess, Denita Hadziabdic, Robert N. Trigiano, Matthew D. Ginzel, William E. Klingeman
Summary: Thousand cankers disease, caused by the fungal pathogen Geosmithia morbida and vectored by the walnut twig beetle, results in tree decline and economic losses. This study presents simplified and inexpensive processes for diagnostic confirmation of the disease, utilizing conventional gel electrophoresis or TaqMan molecular probes for detection of the fungal and insect DNA.
Article
Immunology
Fernando Echegaray, Peter Laing, Samantha Hernandez, Sully Marquez, Amanda Harris, Ian Laing, Adam Chambers, Neil McLennan, Victor A. Sugiharto, Hua-Wei Chen, Sandra Vivero Villagran, Abigail Collingwood, Magelda Montoya, Fausto Bustos Carrillo, Mark P. Simons, Philip J. Cooper, Andrea Lopez, Gabriel Trueba, Joseph Eisenberg, Shuenn-Jue Wu, William Messer, Eva Harris, Josefina Coloma, Leah C. Katzelnick
Summary: The study showed that both existing and newly developed anti-flavivirus rapid diagnostic tests can effectively screen for prior DENV infection to reduce the risk of severe dengue induced by vaccination, and quantitative evaluation can further optimize the rapid diagnostic tests for this purpose.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Erica Frew, Douglas Roberts, Shelly Barry, Matthew Holden, Amanda Restell Mand, Emily Mitsock, Enqing Tan, Wei Yu, Johan Skog
Summary: To address the challenges posed by the novel coronavirus, a cost-effective and rapid SARS-CoV-2 antigen test has been developed, capable of identifying the presence of the virus in patient anterior nares swab samples with high sensitivity and no requirement for equipment or laboratory infrastructure.
SCIENTIFIC REPORTS
(2021)
Article
Pathology
Isabela Medeiros, Charitha Vadlamudi, Katie Doonan, Marisa C. Nielsen, Nancy S. Miller
Summary: Boston Medical Center has implemented a new HIV-1/HIV-2 Qualitative RNA PCR test to replace antibody discrimination testing and diagnose suspected seronegative acute HIV infection. The test algorithm based on this new test may be applicable to other institutions, according to the findings from a 3-month production monitor.
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
(2023)
Article
Virology
Nino Guy Cassuto, Anne Gravier, Mathilda Colin, Aurelie Theillay, Daniela Pires-Roteira, Sandra Pallay, Raphael Serreau, Laurent Hocqueloux, Thierry Prazuck
Summary: The study validates the high sensitivity and specificity of the COVID-VIRO (R) antigen test combined with nasal sampling method, as well as its usability in the general population.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Infectious Diseases
Siau Ek Hwee, Norah Htet Htet, Cho Naing, Wong Siew Tung, Joon Wah Mak
Summary: Leptocheck-WB is a diagnostic test for the detection of acute leptospirosis with moderate sensitivity and specificity. The methodological quality and study design may affect the accuracy of the test.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2022)
Review
Infectious Diseases
D. Bouzid, M. -C. Zanella, S. Kerneis, B. Visseaux, L. May, J. Schrenzel, V. Cattoir
Summary: This study provides an overview of currently available RDTs for infectious diseases in the emergency department setting and their impact on patient care. It categorizes commercially available FDA- or CE-approved assays based on clinical syndromes and describes their performance in clinical validation studies.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Nanoscience & Nanotechnology
Pojchanun Kanitthamniyom, Pei Yun Hon, Aiwu Zhou, Mohammad Yazid Abdad, Zhi Yun Leow, Nurhidayah Binte Mohamed Yazid, Vanessa Lim Wei Xun, Shawn Vasoo, Yi Zhang
Summary: MDM Carba is a magnetic digital microfluidic platform developed for rapid identification of Carbapenemase-producing Enterobacteriaceae (CPE). It offers simple operation, automation, and the potential for manual operation in resource-limited settings for point-of-care testing. The platform showed comparable sensitivity and specificity to the widely used Carba NP test, indicating promising potential for improving diagnostics for antibiotic resistance in clinical settings.
MICROSYSTEMS & NANOENGINEERING
(2021)
Letter
Medicine, General & Internal
Laura E. Maddy, Daniel P. O'Brien, Philip J. Campbell
INTERNAL MEDICINE JOURNAL
(2018)
Article
Public, Environmental & Occupational Health
Cecilia Ferreyra, Daniel O'Brien, Beatriz Alonso, Abdulbasset Al-Zomour, Nathan Ford
CONFLICT AND HEALTH
(2018)
Review
Surgery
Daniel P. O'Brien, Peter Callan, N. Deborah Friedman, Eugene Athan, Andrew Hughes, Anthony McDonald
ANZ JOURNAL OF SURGERY
(2019)
Article
Infectious Diseases
James W. Wynne, Timothy P. Stinear, Eugene Athan, Wojtek P. Michalski, Daniel P. O'Brien
PLOS NEGLECTED TROPICAL DISEASES
(2018)
Article
Infectious Diseases
Arvind Yerramilli, Ee Laine Tay, Andrew J. Stewardson, Janet Fyfe, Daniel P. O'Brien, Paul D. R. Johnson
PLOS NEGLECTED TROPICAL DISEASES
(2018)
Review
Public, Environmental & Occupational Health
D. P. O'Brien, I. Jeanne, K. Blasdell, M. Avumegah, E. Athan
EPIDEMIOLOGY AND INFECTION
(2019)
Editorial Material
Medicine, General & Internal
Daniel P. O'Brien, Maria Globan, Janet M. Fyfe, Caroline J. Lavender, Adrian Murrie, Damian Flanagan, Peter Meggyesy, Jonathan T. Priestley, David Leslie
MEDICAL JOURNAL OF AUSTRALIA
(2019)
Article
Infectious Diseases
Daniel P. O'Brien, Adrian Murrie, Peter Meggyesy, Jonathan Priestley, Avinash Rajcoomar, Eugene Athan
PLOS NEGLECTED TROPICAL DISEASES
(2019)
Review
Immunology
Daniel P. O'Brien, Nathan Ford, Amadou G. Djirmay, Alexandra Calmy, Marco Vitoria, Tomas O. Jensen, Vanessa Christinet
JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES
(2019)
Review
Public, Environmental & Occupational Health
Kai Hodgkin, Grace Joshy, Jenny Browne, Istri Bartini, Terence H. Hull, Kamalini Lokuge
REPRODUCTIVE HEALTH
(2019)
Article
Health Care Sciences & Services
Mafruha Alam, Cathy Banwell, Anna Olsen, Kamalini Lokuge
JMIR MHEALTH AND UHEALTH
(2019)
Article
Immunology
Josephine Davies, Kamalini Lokuge, Kathryn Glass
Article
Health Care Sciences & Services
Mafruha Alam, Cathy Banwell, Kamalini Lokuge
JMIR MHEALTH AND UHEALTH
(2020)
Review
Health Care Sciences & Services
Nicole Rendell, Alexander Rosewell, Kamalini Lokuge, Emma Field
Summary: This study aims to investigate the selection processes of health system performance indicators. The common features of indicator selection processes include literature review, consensus building with stakeholders, structuring indicators into categories, and focus on validity and feasibility. The evidence on field testing, utility, and consideration of reporting burden is less clear.
INTERNATIONAL JOURNAL OF HEALTH POLICY AND MANAGEMENT
(2022)
Article
Immunology
Alex Y. C. Tai, Eugene Athan, N. Deborah Friedman, Andrew Hughes, Aaron Walton, Daniel P. O'Brien
EMERGING INFECTIOUS DISEASES
(2018)