Article
Cell Biology
Jingsi Yang, Nan Ding, Dandan Zhao, Yunsheng Yu, Chunlai Shao, Xuan Ni, Zhen-Ao Zhao, Zhen Li, Jianquan Chen, Zheng Ying, Miao Yu, Wei Lei, Shijun Hu
Summary: The study developed a new strategy of using EBSS treatment to mimic in vivo starvation event, promoting the maturation of hESC-CMs. Intermittent starvation through EBSS significantly enhanced structural, metabolic, and electrophysiological maturity of hESC-CMs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Xianji Liu, Rui Zhao, Qianqian Ding, Xiaoqiang Yao, Suk Ying Tsang
Summary: TRPC7 regulates the automaticity of mESC-CMs through two mechanisms: by positively regulating the intracellular Ca2+ clock through the regulation of activities of RyR2 and SERCA, and by positively regulating the membrane clock via its influence on NCX activity. The expression level of TRPC7 affects the frequency of Ca2+ transients, local Ca2+ releases, and action potentials.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Chemistry, Multidisciplinary
Jia-dan Wang, Ying Shao, Dan Liu, Nuo-ya Liu, Dan-yan Zhu
Summary: In this study, we elucidated the mechanisms of mitochondrial damage in ESC-CMs after knockdown of Rictor using a model of cardiomyocyte differentiation from mouse ES cells. Knockdown of Rictor led to swollen and ruptured mitochondria, decreased ATP production and mitochondrial transmembrane potential, inhibited mitochondrial respiratory chain complex activities, and hindered Cx43 translocation into mitochondria, ultimately resulted in mitochondrial dysfunction in ESC-CMs.
ACTA PHARMACOLOGICA SINICA
(2021)
Article
Cell & Tissue Engineering
Beatrice Xuan Ho, Hongbing Yu, Jeremy Kah Sheng Pang, Jin-Hui Hor, Lee Chuen Liew, Piotr Szyniarowski, Christina Ying Yan Lim, Omer An, Henry He Yang, Colin L. Stewart, Woon Khiong Chan, Shi-Yan Ng, Boon-Seng Soh
Summary: The study found that treatment with IFN-g can promote the maturation of human pluripotent stem cell-derived cardiomyocytes, enhancing their electrophysiological characteristics and sarcomeric structure, and this effect can be nullified with a JAK-STAT pathway inhibitor.
Article
Biology
Mariangela Scalise, Fabiola Marino, Luca Salerno, Teresa Mancuso, Donato Cappetta, Antonella Barone, Elvira Immacolata Parrotta, Annalaura Torella, Domenico Palumbo, Pierangelo Veltri, Antonella De Angelis, Liberato Berrino, Francesco Rossi, Alessandro Weisz, Marcello Rota, Konrad Urbanek, Bernardo Nadal-Ginard, Daniele Torella, Eleonora Cianflone
Summary: The study found that murine adult cardiac stem cells differentiated into contracting cardiomyocytes in vitro, showing miRNA/mRNA expression patterns similar to that of adult cardiomyocytes. miRNAs regulate cardiomyocyte differentiation by targeting cell cycle and DNA replication mRNAs and promoting myogenic commitment. The networks of miRNA/mRNAs in differentiated cardiomyocytes closely resemble those of adult cardiomyocytes.
COMMUNICATIONS BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Julius Niehoff, Matthias Matzkies, Filomain Nguemo, Juergen Hescheler, Michael Reppel
Summary: This study is the first to demonstrate the influence of light on the beating rhythm of heart tissue in vitro. The results indicate that especially the infrared spectrum of light alters the BRV. These findings could be of interest for clinical applications such as the field of optical pacing as well as in neonatal patient care.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Review
Cell & Tissue Engineering
Hao Yang, Yuan Yang, Fedir N. Kiskin, Mengcheng Shen, Joe Z. Zhang
Summary: In recent years, hiPSC-CM cell therapy has gained attention as a potential treatment for heart injuries. However, the mass production of hiPSC-CMs remains challenging and improvements are needed in their proliferation and maturation for clinical applications.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Xiang Zhao, Bethany N. Radford, Mark Ungrin, Wendy Dean, Myriam Hemberger
Summary: Normal developmental progression relies on close interactions between the embryonic and extraembryonic lineages in the pre- and peri-gastrulation stage conceptus. Trophoblast cells of the extraembryonic ectoderm play a role in delaying cardiomyocyte differentiation dynamics and prolonging pluripotency in embryonic stem cells (ESCs). These findings provide insights into the intricate crosstalk between different compartments of the early conceptus and have implications for synthetic embryo research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Qianqian Ding, Xianji Liu, Yanxiang Qi, Xiaoqiang Yao, Suk Ying Tsang
Summary: The study investigated the role of transient receptor potential ankyrin 1 (TRPA1) channel in regulating the maturation of embryonic stem cell-derived cardiomyocytes (ESC-CMs). Results showed that TRPA1 promotes the maturation of cardiomyocytes, and its knockdown impairs maturation. Mechanistically, TRPA1 regulates ESC-CMs maturation through the MKP-1-p38 MAPK-PGC-1a pathway. Activation of TRPA1 provides a novel and straightforward strategy for improving the maturation of pluripotent stem cell-derived cardiomyocytes.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Cell Biology
Seung-Cheol Choi, Ha-Rim Seo, Long-Hui Cui, Myeong-Hwa Song, Ji-Min Noh, Kyung-Seob Kim, Ji-Hyun Choi, Jong-Ho Kim, Chi-Yeon Park, Hyung Joon Joo, Soon Jun Hong, Tae Hee Ko, Jong-Il Choi, Hyo Jin Kim, Jong-Hoon Kim, Se-Hwan Paek, Ji-Na Park, Dong-Hyung Kim, Yongjun Jang, Yongdoo Park, Do-Sun Lim
Summary: The study demonstrates that FGF4 and ascorbic acid can induce differentiation of human embryonic stem cell-cardiogenic mesoderm cells into mature cardiomyocytes, providing a model for adult-onset cardiac disease and drug discovery. These mature cardiomyocytes release acute myocardial infarction biomarkers in response to hypoxic injury, with potential for diagnosis and prognosis in coronary artery diseases based on transcriptome analyses.
Article
Cell Biology
So Young Kim, Jeoung Eun Lee, Sung Hun Kang, So Min Lee, Jiwon Jeon, Dong Ryul Lee
Summary: The study demonstrated that mesenchymal stem cells have protective effects in rats with noise-induced hearing loss by preserving hearing levels and attenuating cell apoptosis.
Review
Biology
Afshin Zare, Aria Salehpour, Arezoo Khoradmehr, Shabnam Bakhshalizadeh, Vahid Najafzadeh, Sahar Almasi-Turk, Mahdi Mahdipour, Reza Shirazi, Amin Tamadon
Summary: More research is being conducted on using stem cell lines to develop into cardiomyocytes for myocardial cell treatments. Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) have shown promise in treating various cardiac illnesses. This study explores the differentiation of these cells into cardiomyocytes from an epigenetic perspective, including histone acetylation, methylation, DNA alterations, RNA methylation, and mitochondrial mutations.
Article
Medicine, Research & Experimental
Julius Niehoff, Matthias Matzkies, Filomain Nguemo, Juergen Hescheler, Michael Reppel
Summary: The study found that melatonin can reduce the beating frequency of the heart, increase beat rate variability, and potentially exhibit toxic effects in an in vitro model. These findings provide important insights into the impact of melatonin on the development of circadian rhythms during the embryonic phase.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Review
Cell & Tissue Engineering
Jessica C. Garbern, Richard T. Lee
Summary: Current methods to differentiate cardiomyocytes from human pluripotent stem cells inadequately recapitulate complete development, resulting in cardiomyocytes with an immature or fetal-like phenotype. Metabolic changes and mitochondrial dynamics play key roles in the maturation of cardiomyocytes, with the transitions in mitochondria responding to metabolic signals throughout development.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell Biology
Rui Zhao, Xianji Liu, Zenghua Qi, Xiaoqiang Yao, Suk Ying Tsang
Summary: The study found that TRPV1 channels regulate the electrophysiological characteristics of ESC-CMs by controlling calcium release from the sarcoplasmic reticulum (SR) and stimulating Na+/Ca2(+) exchanger (NCX) activity. These novel findings reveal an important interplay between TRPV1 and NCX in regulating the physiological functions of ESC-CMs.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)