Article
Biochemistry & Molecular Biology
Asami Nishikori, Yoshito Nishimura, Rei Shibata, Koh-ichi Ohshima, Yuka Gion, Tomoka Ikeda, Midori Filiz Nishimura, Tadashi Yoshino, Yasuharu Sato
Summary: AID expression in IgG4-related diseases is influenced by various factors, and AID may play a key role in driving fibrosis and oncogenesis in ocular adnexal organs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Takashi Iizuka, Kousho Wakae, Masanori Ono, Takuma Suzuki, Yasunari Mizumoto, Kouichi Kitamura, Shin-ichi Horike, Masamichi Muramatsu, Hiroshi Fujiwara
Summary: The study revealed that AID plays a significant role in regulating the expression of GDF-9 and SCF in the ovary, possibly through a GDF-9 and SCF feedback system to modulate communication between oocytes and granulosa cells.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Xia Xie, Tingting Gan, Bing Rao, Weiwei Zhang, Rohit A. Panchakshari, Dingpeng Yang, Xiong Ji, Yu Cao, Frederick W. Alt, Fei-Long Meng, Jiazhi Hu
Summary: The mutant AID-delC protein fails to efficiently target antibody genes, forms condensates, and may exert a dominant-negative effect on wild-type AID.
Review
Genetics & Heredity
Tony M. Mertz, Christopher D. Collins, Madeline Dennis, Margo Coxon, Steven A. Roberts
Summary: The occurrence and development of cancer are closely related to mutations, and dysregulated activity of APOBECs can lead to mutations. The study of mutation signatures helps us understand the patterns and processes of mutations. In humans, APOBEC-generated genetic heterogeneity plays an important role in cancer development, metastasis, and resistance to therapy.
ANNUAL REVIEW OF GENETICS
(2022)
Article
Biochemical Research Methods
Guiyue Tang, Bingran Xie, Xiangna Hong, Hang Qin, Jingfang Wang, Hai Huang, Pei Hao, Xuan Li
Summary: The study successfully separated the dual activities of A3A and created novel human A3A variants with RNA-specific cytidine deaminase activity through the development of a reporter system and rational mutagenesis design.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Medicine, General & Internal
Shohei Kikuchi, Akinori Wada, Yusuke Kamihara, Yoshimi Nabe, Tomoki Minemura, Jun Murakami, Nam H. H. Dang, Tsutomu Sato
Summary: Biclonal gammopathy is a rare phenomenon characterized by the detection of 2 M proteins in the same patient. The etiology of this condition remains unclear. In this study, we found double positive cells in two cases of B-cell malignancy with biclonal gammopathy, suggesting a possible involvement of cell transition and class switch recombination. Immunostaining revealed the expression of activation induced cytidine deaminase in these cells, indicating its potential role in the pathogenesis of biclonal gammopathy.
Article
Biotechnology & Applied Microbiology
Baole Qu, Yun Zhao, Lili An, Haiying Hang
Summary: This study demonstrates that adenine deaminase (ADA) can efficiently induce random A/T to G/C mutations on the target gene in CHO cell display, and can be applied in affinity maturation to improve antibody affinity. The combined use of ADA and activation-induced cytidine deaminase (AID) provides more mutant types, offering an opportunity to acquire new mutants with higher affinities than those obtained by only using AID.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Feixuan Li, Xiao-Yu Liu, Lei Ni, Fan Jin
Summary: Researchers developed a method called AIDmut-Seq to detect transcription factor targets on the genome. It involves simple steps and has been validated for various transcriptional activators. However, it has lower efficiency for some small transcriptional repressors. Despite potential false-positive and false-negative results, AIDmut-Seq shows promise as a complementary tool for studying protein-DNA interactions.
MICROBIOLOGY SPECTRUM
(2023)
Review
Immunology
Junna Jiao, Zhuangwei Lv, Yurong Wang, Liye Fan, Angang Yang
Summary: Activation-induced cytidine deaminase (AID) plays a crucial role in promoting B cell diversification through somatic hypermutation (SHM) and class switch recombination (CSR). Apart from its physiological function of humoral immune response, AID has been linked to the initiation and progression of lymphomas. This review proposes an alternative role for AID and explores its off-target effects in regulating tumorigenesis.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Junna Jiao, Zhuang Qian, Yurong Wang, Mei Liu, Liye Fan, Mengqing Liu, Zichen Hao, Junrong Jiao, Zhuangwei Lv
Summary: Sonodynamic therapy using MWO4-PEG NPs shows promising inhibitory effects on activation-induced cytidine deaminase (AID) in diffuse large B-cell lymphoma. The interaction between reactive oxygen species and AID was investigated, and the NPs were found to effectively neutralize the extensive carcinogenesis caused by AID. Cell apoptosis and ROS generation in DLBCL cells treated with these NPs were observed to confirm their sonosensitivity and toxicity, indicating their potential as a treatment for AID-positive hematopoietic cancers.
Article
Medicine, General & Internal
Ege Sinan Torun, Aynur Daglar Aday, Meliha Nalcaci
Summary: The study found that AID expression is significantly increased in the peripheral blood of MDS patients compared to healthy controls. However, there was no significant difference in AID expression in lower risk and higher risk subgroups, as well as in patients treated with hypomethylating agents. This suggests that increased AID expression may be an early step in MDS pathogenesis.
TURKISH JOURNAL OF MEDICAL SCIENCES
(2021)
Article
Cell Biology
Marc Descatoire, Remi Fritzen, Samuel Rotman, Genevieve Kuntzelman, Xavier Charles Leber, Stephanie Droz-Georget, Adrian J. Thrasher, Elisabetta Traggiai, Fabio Candotti
Summary: A main feature of Wiskott-Aldrich syndrome is increased susceptibility to autoimmunity, with WAS protein playing a key role in controlling peripheral tolerance in germinal center B cells, leading to the development of self-reactive antibodies and kidney pathology.
Article
Oncology
Grant Godsmark, Leonardo Alves De Souza Rios, Shaheen Mowla
Summary: The study demonstrated that AID is highly specific to B-cell-derived cancers, with ectopic overexpression leading to rapid cell death, and silencing AID significantly impacting genomic stability, proliferation, migration, and drug resistance. AID is identified as an important driver of lymphoma, affecting multiple cellular events, and potentially a strong candidate for targeted therapy in lymphoma.
ANTICANCER RESEARCH
(2021)
Article
Oncology
Sarrah L. Hasanali, Daley S. Morera, Ronny R. Racine, Martin Hennig, Santu Ghosh, Luis E. Lopez, Marie C. Hupe, Diogo O. Escudero, Jiaojiao Wang, Huabin Zhu, Semih Sarcan, Ijeoma Azih, Michael Zhou, Andre R. Jordan, Martha K. Terris, Markus A. Kuczyk, Axel S. Merseburger, Vinata B. Lokeshwar
Summary: This study identified that the splice variant V1 of HYAL-4 is upregulated in bladder cancer and drives a malignant phenotype, as well as chemotherapy resistance to gemcitabine. While V1 does not affect gemcitabine influx, it increases drug metabolism and efflux by upregulating CD44-JAK2/STAT3 signaling, leading to resistance.
CLINICAL CANCER RESEARCH
(2021)
Article
Cell Biology
Lin Sun, Xiaohong Zhao, Xindong Liu, Bo Zhong, Hong Tang, Wei Jin, Hans Clevers, Hui Wang, Xiaohu Wang, Chen Dong
Summary: The transcription factor Ascl2 is upregulated in GC B cells and promotes their development, antibody production, and affinity maturation. Deletion of Ascl2 impairs GC response, but ectopic expression of AID in Ascl2-deficient B cells can rescue antibody defects. Thus, Ascl2 regulates AID transcription and enhances GC B cell responses.
Article
Multidisciplinary Sciences
Yosuke Kurashima, Takaaki Kigoshi, Sayuri Murasaki, Fujimi Arai, Kaoru Shimada, Natsumi Seki, Yun-Gi Kim, Koji Hase, Hiroshi Ohno, Kazuya Kawano, Hiroshi Ashida, Toshihiko Suzuki, Masako Morimoto, Yukari Saito, Ai Sasou, Yuki Goda, Yoshikazu Yuki, Yutaka Inagaki, Hideki Iijima, Wataru Suda, Masahira Hattori, Hiroshi Kiyono
Summary: The study demonstrates that pancreatic GP2 can protect the intestinal epithelial barrier from bacterial invasion and is linked to prevention of Crohn's disease. Pancreatic GP2 is the source of intestinal luminal GP2, binding bacteria to prevent their attachment to the epithelium, which also reduces pathology in a DSS colitis mouse model.
NATURE COMMUNICATIONS
(2021)
Article
Urology & Nephrology
Federica Barutta, Shunsuke Kimura, Koji Hase, Stefania Bellini, Beatrice Corbetta, Alessandro Corbelli, Fabio Fiordaliso, Antonella Barreca, Mauro Giulio Papotti, Gian Marco Ghiggeri, Gennaro Salvidio, Dario Roccatello, Valentina Audrito, Silvia Deaglio, Roberto Gambino, Stefania Bruno, Giovanni Camussi, Miriam Martini, Emilio Hirsch, Marilena Durazzo, Hiroshi Ohno, Gabriella Gruden
Summary: The study found that the M-Sec?TNT system plays an important protective role in podocytes by rescuing them via mitochondrial horizontal transfer. Overexpression of M-Sec in FSGS suggests a cytoprotective role, while M-Sec deletion leads to progressive FSGS development. These findings provide insight into potential therapeutic targets for FSGS and suggest regenerative cell-based therapy options through TNT-mediated organelle transfer.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2021)
Article
Multidisciplinary Sciences
Seiga Komiyama, Takahiro Yamada, Nobuyuki Takemura, Norihiro Kokudo, Koji Hase, Yuki Kawamura
Summary: Liver cancer is the fourth leading cause of cancer-related death, with patients exhibiting distinct tumor microbiota composition that may play a role in the pathogenesis of HCC.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Jianshi Jin, Reiko Yamamoto, Tadashi Takeuchi, Guangwei Cui, Eiji Miyauchi, Nozomi Hojo, Koichi Ikuta, Hiroshi Ohno, Katsuyuki Shiroguchi
Summary: The study develops a method called Barcoding Bacteria for Identification and Quantification (BarBIQ) that allows for the classification and quantification of individual bacterial cells in the microbiota. It is found that BarBIQ reveals location-dependent differences in the microbiota, which cannot be shown by conventional 16S rRNA gene-amplicon sequencing methods.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Goo-Young Seo, Daisuke Takahashi, Qingyang Wang, Zbigniew Mikulski, Angeline Chen, Ting-Fang Chou, Paola Marcovecchio, Sara McArdle, Ashu Sethi, Jr-Wen Shui, Masumi Takahashi, Charles D. Surh, Hilde Cheroutre, Mitchell Kronenberg
Summary: This study reveals the important role of intestinal epithelial cells and basement membrane in the survival and function of Intraepithelial T cells (IETs). The binding of the ligand LIGHT to the herpes virus entry mediator (HVEM) expressed by epithelial cells promotes the survival of IETs. Additionally, epithelial cells increase the synthesis of basement membrane protein collagen IV, which interacts with beta(1) integrins expressed by IETs. These interactions are crucial for the maintenance and protective function of IETs in mucosal immunity.
SCIENCE IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tamotsu Kato, Masaharu Kagawa, Wataru Suda, Yuuri Tsuboi, Sayo Inoue-Suzuki, Jun Kikuchi, Masahira Hattori, Toshiko Ohta, Hiroshi Ohno
Summary: After fructo-oligosaccharide (FOS) supplementation, changes in the gut ecosystem, immune system, and metabolome were observed, showing individual variability. Studying FOS could help accumulate personalized knowledge of the gut ecosystem, potentially offering more effective health guidance for prebiotic consumption in the future.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Ryuji Ohue-Kitano, Hazuki Nonaka, Akari Nishida, Yuki Masujima, Daisuke Takahashi, Takako Ikeda, Akiharu Uwamizu, Miyako Tanaka, Motoyuki Kohjima, Miki Igarashi, Hironori Katoh, Tomohiro Tanaka, Asuka Inoue, Takayoshi Suganami, Koji Hase, Yoshihiro Ogawa, Junken Aoki, Ikuo Kimura
Summary: Medium-chain triglycerides (MCTs) are unique forms of dietary fat that have various health benefits. A receptor called G protein-coupled 84 (GPR84) acts as a receptor for medium-chain fatty acids (MCFAs), but its role in nutritional signaling is still unclear. This study found that GPR84 plays a protective role in hepatic functions by sensing increases in MCFAs and suppressing lipotoxicity-induced macrophage overactivation. Additionally, stimulating GPR84 or administering MCTs or MCFAs improved nonalcoholic steatohepatitis (NASH) in mouse models, suggesting a potential strategy for treating NASH.
Article
Genetics & Heredity
Hiroshi Mori, Tamotsu Kato, Hiroaki Ozawa, Mitsuo Sakamoto, Takumi Murakami, Todd D. Taylor, Atsushi Toyoda, Moriya Ohkuma, Ken Kurokawa, Hiroshi Ohno
Summary: To understand the biases in human gut microbiome studies, a reference microbiome composed of an artificial mock community is crucial. However, there is a lack of suitable mock communities that accurately represent the human gut microbiome. In this study, we created a novel mock community consisting of 18 major bacterial species found in the human gut and examined the impact of experimental and bioinformatics procedures on 16S rRNA gene and shotgun metagenomic sequencing. Our findings revealed that DNA extraction methods significantly influenced DNA yields and taxonomic composition of sequenced reads and certain commonly used primers for 16S rRNA genes led to underestimation of certain gut commensal taxa. Additionally, we demonstrated that the binning of shotgun metagenomic sequences using MetaBAT2 produced consistent, less-contaminated bins with varying completeness. The use of multiple binning tools with MetaWRAP improved completeness but sometimes increased contamination rate. This benchmark study provides a crucial foundation for interpreting human gut microbiome data, allowing for standardization among different methodologies, and facilitating the advancement of analytical methods.
Article
Biotechnology & Applied Microbiology
Futo Asano, Arisa Tsuboi, Shigeharu Moriya, Tamotsu Kato, Naoko Tsuji, Teruno Nakaguma, Hiroshi Ohno, Hirokuni Miyamoto, Hiroaki Kodama
Summary: The study found that incorporating thermophilic bacteria-fermented compost derived from marine animals into the humus can enhance the growth of Hercules beetle larvae, especially the females. This compost alters the gut microflora of the larvae, leading to improved growth.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Review
Immunology
Eiji Miyauchi, Chikako Shimokawa, Alex Steimle, Mahesh S. Desai, Hiroshi Ohno
Summary: The prevalence of autoimmune diseases has increased rapidly worldwide. Besides traditional risk factors, alterations in gut microbiota have been identified as important factors influencing autoimmune responses and disease outcome in extra-intestinal autoimmune diseases.
NATURE REVIEWS IMMUNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Wei Zhe, Naomi Hoshina, Yukihiro Itoh, Toshifumi Tojo, Takayoshi Suzuki, Koji Hase, Daisuke Takahashi
Summary: This study evaluated the therapeutic effect of a novel selective HDAC1 inhibitor TTA03-107 in arthritis models and found that it can attenuate the severity of autoimmune arthritis. The results demonstrate that TTA03-107 can slow down the development of arthritis by inhibiting the differentiation and activation of macrophages and Th17 cells.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2022)
Article
Biochemistry & Molecular Biology
Takaaki Miyata, Takayasu Mizushima, Nobuyuki Miyamoto, Takahiro Yamada, Koji Hase, Michihiro Fukushima, Naomichi Nishimura, Shingo Hino, Tatsuya Morita
Summary: Dietary factors can affect the abundance of Akkermansia muciniphila in the colon, with foods high in sulfated sugars and threonine promoting the proliferation of the bacteria.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2022)
Article
Immunology
Yingqian Li, Yue Tang, Jun Liu, Xin Meng, Ying Wang, Qing Min, Rongjian Hong, Takeshi Tsubata, Koji Hase, Ji-Yang Wang
Summary: The study reveals the important role of GMFG in S1P-induced MZB chemotaxis, with GMFG acting as an effector downstream of S1P receptors. Serine phosphorylation of GMFG plays a crucial role in this process. Gmfg(-/-) mice show elevated levels of IgM specific to the T-I antigen NP-Ficoll, along with dysregulated MZB localization.
INTERNATIONAL IMMUNOLOGY
(2022)
Review
Immunology
Daisuke Takahashi, Shunsuke Kimura, Koji Hase
Summary: The intestinal immune system collaborates with various immune cell subsets to maintain intestinal homeostasis with the help of bacterial components, metabolites, and nutritional signals. Specialized M cells in GALT facilitate immune surveillance on the mucosal surface, but there is a self-regulatory mechanism to control their hyperplasia to prevent excessive immune response.
INTERNATIONAL IMMUNOLOGY
(2021)