Review
Medicine, General & Internal
Jing Hong Loo, Zhaoran Wang, Rachel S. S. Chong
Summary: Caveolin-1 (Cav-1) is an important membrane protein found in most cell types. It plays a significant role in ocular function and its mutations are associated with a genetic risk of glaucoma development. Cav-1 is involved in both IOP-related and independent mechanisms, affecting vascular dysregulation and leading to impaired neurovascular coupling in glaucoma.
FRONTIERS IN MEDICINE
(2023)
Article
Multidisciplinary Sciences
Danielle L. Michell, Waled A. Shihata, Karen L. Andrews, Nurul Aisha Zainal Abidin, Ann-Maree Jefferis, Amanda K. Sampson, Natalie G. Lumsden, Olivier Huet, Marie-Odile Parat, Garry L. Jennings, Robert G. Parton, Kevin J. Woollard, David M. Kaye, Jaye P. F. Chin-Dusting, Andrew J. Murphy
Summary: High intraluminal pressure can lead to vascular inflammation and subsequent hypertension development; Studies have shown that deficiency of caveolin-1 can reduce pressure-induced inflammatory responses and leukocyte adhesion; These findings demonstrate that Cav1 plays an important role in pressure-induced inflammation.
SCIENTIFIC REPORTS
(2021)
Review
Pharmacology & Pharmacy
Hui Li, Shiyu Du, Panpan Niu, Xiaosong Gu, Jun Wang, Ying Zhao
Summary: VAP-1 plays a role in atherosclerosis, sVAP-1 has potential value for the diagnosis and prognosis of cardiovascular diseases, and VAP-1 inhibitors show promise in slowing the progression of atherosclerosis.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zhenzhu Hou, Wanyan Shi, Jinan Feng, Wei Wang, Enrun Zheng, Hanbin Lin, Cheng Yu, Lisheng Li
Summary: Some deubiquitinating enzymes can regulate their own protein stability in an enzymatic activity- and homomeric interaction-dependent manner, such as USP29 which deubiquitinates itself to protect against degradation, with the N-terminal region critical for its stability. This provides new insights into the diverse regulation of DUBs.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Oncology
Andrej Panic, Henning Reis, Alina Wittka, Christopher Darr, Boris Hadaschik, Verena Jendrossek, Diana Klein
Summary: High expression of CAV1 within penile tumor cells and low expression within the stromal compartment are associated with decreased overall survival of penile cancer patients. This indicates that the shift in stromal-epithelial CAV1 is functionally relevant to tumor progression in penile squamous cell carcinomas.
FRONTIERS IN ONCOLOGY
(2021)
Review
Medical Laboratory Technology
Kai Hou, Shuai Li, Meng Zhang, Xuping Qin
Summary: Caveolin-1 is a significant pathophysiological factor in atherosclerosis, potentially influencing autophagy activation. Understanding the exact mechanisms and roles of caveolin-1 in autophagic flux can lead to novel therapeutic approaches for atherosclerosis.
CLINICA CHIMICA ACTA
(2021)
Article
Multidisciplinary Sciences
Ying Zhao, Wusheng Zhu, Ting Wan, Xiaohao Zhang, Yunzi Li, Zhenqian Huang, Pengfei Xu, Kangmo Huang, Ruidong Ye, Yi Xie, Xinfeng Liu
Summary: This study reveals that vascular abnormality may compromise myelin maintenance and regeneration through the loss of endothelial Cav-1, providing a potential intercellular mechanism in ischemic demyelination.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Shuai Wang, Xiaoning Liu, Zeqi Meng, Qi Feng, Yanling Lin, Honglin Niu, Chao Yu, Yanhong Zong, Lingling Guo, Weiwei Yang, Yuehua Ma, Wenjun Zhang, Chenyang Li, Yunran Yang, Wenjuan Wang, Xurui Gao, Yaxin Hu, Chao Liu, Lei Nie
Summary: DCBLD2 is a transmembrane protein that regulates PDGFR-beta endocytosis, and its inhibition of receptor endocytosis can regulate the phenotypic conversion of VSMCs and arterial wall proliferation, making it a potential therapeutic target for cardiovascular diseases.
Article
Biochemistry & Molecular Biology
Sunday N. Okafor, Abigail Meyer, Jay Gadsden, Fadi Ahmed, Lilian Guzman, Hashim Ahmed, Jose A. Fernandez Romero, Pavimol Angsantikul
Summary: This study screened FDA-approved and investigational drugs for their potential as HIV-1 protease inhibitors. The findings revealed that CBR003PS and CBR013PS exhibited significant binding affinity to the HIV-1 protease with high stability, showing potential for repurposing as HIV-1 protease inhibitors.
Article
Multidisciplinary Sciences
Qiankun Wang, Hongbo Gao, Kolin M. Clark, Christian Shema Mugisha, Keanu Davis, Jack P. Tang, Gray H. Harlan, Carl J. DeSelm, Rachel M. Presti, Sebla B. Kutluay, Liang Shan
Summary: HIV-1's rapid evolution and high mutation rates enable it to evade the host immune system, but intervention targeting the protease activity with the inflammasome sensor CARD8 has shown promise in clearing latent HIV-1 infection.
Article
Biotechnology & Applied Microbiology
Feng Luo, Jinbang Li, Jihong Liu, Kunping Liu
Summary: This study evaluated the effects and mechanisms of the WNT/beta-catenin signaling pathway on chemoresistance in colorectal cancer cells. Inhibition of the WNT/beta-catenin/TCF12/caveolin-1 pathway provides a promising therapeutic strategy for colorectal cancer treatment.
CANCER GENE THERAPY
(2022)
Review
Pharmacology & Pharmacy
Shilu Luo, Ming Yang, Hao Zhao, Yachun Han, Na Jiang, Jinfei Yang, Wei Chen, Chenrui Li, Yan Liu, Chanyue Zhao, Lin Sun
Summary: Caveolin-1 (Cav-1) plays a crucial role in regulating cellular endocytosis, stress responses, and signal transduction. It is involved in mediating the endocytosis of albumin, cholesterol, and glucose, participating in cellular metabolic reprogramming, and contributing to the progression of kidney disease. Cav-1 also serves as a potential therapeutic target for the treatment of kidney disease.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Food Science & Technology
Yu Wang, Jialei Sun, Yingwang Deng, Yuanqiang Tu, Haiyue Niu, Wenjing Cai, Xue Han
Summary: The heat-resistant protease secreted by Pseudomonas in raw milk is a key factor affecting the quality of dairy foods. The protease from P. fluorescens W3 is highly active in milk, with whey protein content influencing its activity. AprX is the main protease secreted by P. fluorescens W3, with the ability to hydrolyze casein. Whey protein can enhance the expression of the aprX gene by P. fluorescens W3.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Review
Multidisciplinary Sciences
Yan Shu, Si Jin
Summary: Atherosclerosis (AS) is a chronic vascular disease characterized by lipid accumulation and inflammation, and it is the leading cause of death nationwide. Endothelial cell activation and dysfunction, triggered by pro-inflammatory agonists, oxidized lipoproteins, and biomechanical stimuli, play a crucial role in AS development. The membrane protein Cav1, highly expressed in endothelial cells, regulates lipid transport, inflammatory responses, and cellular signaling pathways, and has been implicated in atherogenesis. This review summarizes recent studies on Cav1's structure and functions, and its potential mechanisms in AS development, providing insights for AS prevention and therapeutic strategies.
Article
Biochemistry & Molecular Biology
Dongdong Fu, Shuai Wu, Xiangfu Jiang, Tingyu You, Yu Li, Jiao Xin, Xiaowen Feng, Jiagen Wen, Yan Huang, Chengmu Hu
Summary: The study aimed to observe the effects of APAP on the vasculature in NAFLD and determine if CAV1 could alleviate vascular oxidative stress and inflammation by targeting Ang II or its downstream pathways. The results showed that CAV1 exerts a protective effect against vascular injury by inhibiting oxidative stress and inflammation through the PKC/MAPK pathway.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Oncology
Sonja Levy, Aoife B. Kilgallen, Catharina M. Korse, Marish I. F. J. Oerlemans, Joost P. G. Sluijter, Linda W. van Laake, Gerlof D. Valk, Margot E. T. Tesselaar
Summary: This study validated NT-proBNP as the superior biomarker for CHD. Patients with elevated serotonin levels and NT-proBNP levels between 6.5 and 27 pmol/L, and specifically >27 pmol/L, should be monitored closely for the development of CHD.
Article
Biology
Joseph Wayne M. Fowler, Rong Zhang, Bo Tao, Nabil E. Boutagy, William C. Sessa
Summary: The relationship between cholesterol homeostasis and immunity in leukocytes has been extensively studied, but not much is known about this relationship in vascular endothelium cells. This study reveals the role of cholesterol and the transcription regulator SREBP2 in the inflammatory response of endothelial cells.
Article
Biochemistry & Molecular Biology
Max J. M. Silvis, Evelyne J. J. Demkes, Leo Timmers, Fatih Arslan, Saskia C. A. de Jager, Joost P. G. Sluijter, Arend Mosterd, Dominique P. V. de Kleijn, Lena Bosch, Gerardus P. J. van Hout
Summary: This study investigated the effects of the NLRP3-inflammasome inhibitor IZD334 on cardiac damage in a pig model of myocardial infarction. The results showed that IZD334 successfully reduced IL-1 beta release from stimulated porcine blood cells in vitro, but did not attenuate the inflammatory response or improve cardiac function in vivo.
Article
Biology
Patricia van den Hoogen, Manon M. H. Huibers, Floor W. van den Dolder, Roel de Weger, Erica Siera-de Koning, Marish I. F. Oerlemans, Nicolaas de Jonge, Linda W. van Laake, Pieter A. Doevendans, Joost. P. G. Sluijter, Aryan Vink, Saskia C. A. de Jager
Summary: Heart transplantation is the gold standard for patients with end-stage heart failure, but immune responses to the transplanted heart can lead to complications and graft failure. Elevated levels of antibodies prior to transplantation are associated with poor outcomes after transplantation. Specifically, higher levels of IgG1 and IgG2 antibodies are associated with increased rejection of the transplanted heart and remain elevated after transplantation. This suggests that heart transplant patients with a more active antibody-mediated immune response might benefit from expanded immunosuppressive therapy.
Article
Engineering, Biomedical
Madison Jade Ainsworth, Nino Chirico, Mylene de Ruijter, Andrei Hrynevich, Inge Dokter, Joost P. G. Sluijter, Jos Malda, Alain van Mil, Miguel Castilho
Summary: In order to develop thick engineered cardiac tissue construct with a pre-vascularization pathway, a combination of extrusion-based bioprinting and melt electrowriting techniques was investigated. Gelatin methacryloyl (GelMA) was used as a base hydrogel and collagen, Matrigel, and fibrinogen were utilized as interpenetrating polymers to support myocardial functionality. An anatomically inspired vascular pathway was implemented in a dual extrusion-based bioprinting setup. The optimized bioinks effectively filled the microfiber mesh and maintained cell viability, and distinct cell populations were visualized in their respective patterns up to D14.
Article
Biology
Carlos Castrillon, Lea Simoni, Theo van den Broek, Cees van der Poel, Elliot H. Akama-Garren, Minghe Ma, Michael C. Carroll
Summary: This study characterized the post-germinal center B cell compartment in a new mouse model of autoimmunity using single-cell transcriptomics and antibody repertoire analysis. The results revealed multiple subclusters of antibody-secreting cells and memory B cells, each with distinct characteristics and in vivo localization. Additionally, a clonal redundancy was observed within these subsets, suggesting a potential mechanism for the perpetuation of self-reactivity in autoimmune diseases.
Review
Developmental Biology
Renee G. C. Maas, Floor W. van den Dolder, Qianliang Yuan, Jolanda van der Velden, Sean M. Wu, Joost P. G. Sluijter, Jan W. Buikema
Summary: Developmental research has revealed the key roles of Wnt and Hippo signaling in heart growth, but there are currently no clinically relevant therapies for cardiomyopathies. Understanding the decline in proliferation in adult hearts and finding new strategies for cardiac regeneration remain important. Multiple signaling pathways that regulate cardiomyocyte proliferation have been identified and upregulated in injured hearts. This review highlights the interaction of signaling pathways in heart development and their translation into current technologies for cardiomyocyte production.
Article
Biochemistry & Molecular Biology
Joseph Wayne M. Fowler, Nabil E. Boutagy, Rong Zhang, Daiki Horikami, Michael B. Whalen, Casey E. Romanoski, William C. Sessa
Summary: The transcription factor SREBP2 is the main regulator of cholesterol homeostasis and has been implicated in leukocyte immune responses. In this study, the role of SREBP2 in endothelial cells was investigated, revealing its impact on inflammatory chemokine production and interferon response genes.
JOURNAL OF LIPID RESEARCH
(2023)
Article
Engineering, Biomedical
Tom C. L. Bracco Gartner, Ye Wang, Laurynas Leiteris, Iris van Adrichem, Judith Marsman, Marie Jose Goumans, Carlijn V. C. Bouten, Joost P. G. Sluijter, Jaap M. J. den Toonder, Willem J. L. Suyker, Jesper Hjortnaes
Summary: Cardiac fibroblasts in the ever-beating human heart remain quiescent due to the antifibrotic effect of cyclic strain conditions revealed by a novel platform for studying cardiac fibrosis-on-a-chip. This study provides insights into the mechanosensitive pathways and genes involved in the fibrogenic process, which can contribute to the development of new therapies against cardiac fibrosis.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2023)
Article
Multidisciplinary Sciences
Wenping Zhou, Wenxue Li, Shisheng Wang, Barbora Salovska, Zhenyi Hu, Bo Tao, Yi Di, Ujwal Punyamurtula, Benjamin E. E. Turk, William C. C. Sessa, Yansheng Liu
Summary: The authors used optogenetics and mass spectrometry-based phosphoproteomics to study the effects of different intensities, durations, and patterns of Akt1 activation on phosphorylation circuits, revealing downstream signaling outcomes of Akt1. The study showed that different intensities, durations, and patterns of Akt1 activation lead to distinct phosphorylation profiles in vascular endothelial cells, and also revealed the interaction between Akt1 signaling and growth factor signaling in these cells. The resulting dataset provides an important resource for future studies on AKT signaling and dynamics.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Ye Zeng, Mariona Estape Senti, M. Clara I. Labonia, Panagiota Papadopoulou, Maike A. D. Brans, Inge Dokter, Marcel H. Fens, Alain van Mil, Joost P. G. Sluijter, Raymond M. Schiffelers, Pieter Vader, Alexander Kros
Summary: This study demonstrates the efficient delivery of mRNA-LNPs to difficult-to-transfect cardiomyocytes using fusogenic coiled-coil peptides. Local administration via intramyocardial injection significantly enhances mRNA delivery and protein expression. These findings have the potential to advance regenerative therapies for heart failure.
Article
Cardiac & Cardiovascular Systems
Vera Loen, Agnieszka Smoczynska, Alfonso Aranda Hernandez, Coert O. S. Scheerder, Britt H. R. van der Linde, Henriette D. M. Beekman, Aina Cervera-Barea, Gerard J. J. Boink, Joost P. G. Sluijter, Marcel A. G. van der Heyden, Mathias Meine, Marc A. Vos
Summary: This study investigated the relationship between automated determination of short-term variability (STV-ARIauto) of repolarization on intracardiac electrograms and arrhythmias. The results showed that STV-ARIauto was able to predict the occurrence of arrhythmias in a porcine model of acute myocardial infarction. Additionally, the study found that high-rate pacing did not improve arrhythmic outcomes. The findings highlight the potential of using STV-ARIauto for monitoring arrhythmic risk and initiating preventive strategies.
Review
Gastroenterology & Hepatology
Michele A. Rodrigues, Dawidson A. Gomes, Romina Fiorotto, Mateus T. Guerra, Jittima Weerachayaphorn, Tao Bo, William C. Sessa, Mario Strazzabosco, Michael H. Nathanson
Summary: Fluid and bicarbonate secretion in cholangiocytes is regulated by ITPR3, which localizes to the apical region. This localization is dependent on intact lipid rafts and interactions with CAV1 and MYH9. Disruption of lipid rafts or knockdown of CAV1 or MYH9 impairs ITPR3 localization and secretion.
HEPATOLOGY COMMUNICATIONS
(2022)
Review
Medicine, Research & Experimental
Nabil E. Boutagy, Abhishek K. Singh, William C. Sessa
Summary: Obesity is a major contributor to insulin resistance and type 2 diabetes, increasing the risk of cardiovascular disease. Metabolic changes caused by obesity negatively affect the vasculature, exacerbating disease progression. Understanding the cellular and molecular processes is crucial for optimizing medical therapies.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Cardiac & Cardiovascular Systems
Qianliang Yuan, Renee G. C. Maas, Ellen C. J. Brouwer, Jiayi Pei, Christian Snijders Blok, Marko A. Popovic, Nanne J. Paauw, Niels Bovenschen, Jesper Hjortnaes, Magdalena Harakalova, Pieter A. Doevendans, Joost P. G. Sluijter, Jolanda van der Velden, Jan W. Buikema
Summary: This study investigates the disassembly of sarcomeres during mitosis in cardiomyocytes and the effects of CHIR99021 on hiPSC-CMs. The study reveals the activation of Wnt and Hippo signaling in proliferative hiPSC-CMs and the increased efficiency of non-viral vector incorporation by CHIR99021. The findings provide a valuable tool for gene manipulation studies in hiPSC-CMs and engineered cardiac tissue.
JOURNAL OF CARDIOVASCULAR DEVELOPMENT AND DISEASE
(2022)