Article
Cell Biology
Ching-Fang Chang, Kari M. Brown, Yanfen Yang, Samantha A. Brugmann
Summary: Primary cilia, microtubule-based cellular organelles, play a critical role in cellular function and their dysfunction is associated with various disorders. The C2cd3 protein is essential for ciliogenesis and mutations in this gene are linked to certain ciliopathies. Genetic background was found to influence the phenotypic variation in mice models, suggesting a complex interplay between genetic factors and disease manifestation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Genetics & Heredity
Tomas J. Steeman, Juan A. Rubiolo, Laura E. Sanchez, Nora B. Calcaterra, Andrea M. J. Weiner
Summary: The neural crest is a multipotent cell population that migrates extensively and differentiates into various tissues in vertebrate embryos. Studies suggest a potential role for microRNA-145 (miR-145) in controlling Sox9b expression during zebrafish chondrocyte differentiation.
Article
Biology
Justin Ma, Lian Bi, James Spurlin, Peter Lwigale, Carole LaBonne
Summary: During cornea development, the upregulation of nephronectin (Npnt) plays a crucial role in promoting cell migration into the cornea extracellular matrix by functioning as a substrate for integrin alpha 8-positive periocular neural crest (pNC) cells.
Article
Neurosciences
Gang Feng, Yuhua Sun
Summary: The study reveals that the PRC1 complex plays an important role in the development of the central nervous system and enteric nervous system in vertebrates. Loss of function of the rnf2 gene, which encodes the enzymatic component of PRC1, leads to abnormal migration and differentiation of neural precursor cells, as well as defective development of ENS and CNS in zebrafish.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Developmental Biology
Andre L. P. Tavares, Karyn Jourdeuil, Karen M. Neilson, Himani D. Majumdar, Sally A. Moody
Summary: The research identified that Sobp may interact with Six1 and play a crucial role in vertebrate craniofacial development. Sobp interferes with transcriptional activation of Six1+Eya1 target genes and altering Sobp levels in Xenopus embryos disrupts otic vesicle development and causes craniofacial cartilage defects. These findings suggest that Sobp modifies Six1 function and is a potential candidate gene for BOR.
Article
Cell Biology
Karyn Jourdeuil, Karen M. Neilson, Helene Cousin, Andre L. P. Tavares, Himani D. Majumdar, Dominique Alfandari, Sally A. Moody
Summary: Zmym4 plays an important role in regulating embryonic cranial gene expression, and its loss results in altered early cranial gene expression as well as abnormal craniofacial cartilage morphology.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biology
Wenghong Wong, Yike Huang, Zhuanbin Wu, Yu Kong, Jing Luan, Qiaoan Zhang, Jiewen Pan, Kexiang Yan, Zhenghua Zhang
Summary: The mvda gene plays an essential role in the early development of zebrafish, with its deficiency leading to abnormalities in epidermis, angiogenesis, and apoptosis. This study provides insights into the biological function of the human MVD gene.
BIOLOGICAL RESEARCH
(2021)
Article
Multidisciplinary Sciences
Swapnalee Sarmah, Matthew R. Hawkins, Priyadharshini Manikandan, Mark Farrell, James A. Marrs
Summary: The E26 transformation specific (ETS) family transcription factor ELF3 plays a critical role in the development of epidermal, mesenchymal, and neural tissues in zebrafish. Loss of Elf3 function leads to defects in notochord, craniofacial cartilage, and fin development, as well as neural abnormalities. Disruption of extracellular matrix organization and dysregulation of genes encoding ECM proteins and matrix metalloproteases (MMP) may contribute to the observed phenotypes in Elf3-morphants.
Article
Cell Biology
Aubrey G. A. Howard, Aaron C. Nguyen, Joshua Tworig, Priya Ravisankar, Eileen W. Singleton, Can Li, Grayson Kotzur, Joshua S. Waxman, Rosa A. Uribe
Summary: Neural crest cells (NCCs) are migratory and multipotent stem cells essential to vertebrate embryonic development. This study identifies the gene Hoxb5b as a regulator of NCC development, demonstrating that elevated Hoxb5b levels promote expansion of zebrafish NCCs and specifically expand expression domains of vagal NCC markers. The study also shows that early increase in vagal NCCs leads to supernumerary enteric neural progenitors, but these fail to properly expand into enterically fated neurons in the gut tissue.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Xiaolin Jin, Junjun Liu, Shuo Wang, Jiale Shi, Chengtian Zhao, Haibo Xie, Yunsi Kang
Summary: The multifunctional transcription factor E2f4 plays an important role in zebrafish development, particularly in otolith formation and ciliary beating. Its absence leads to abnormal expression of ciliary motility genes, growth retardation, intestinal microvilli damage, reduced cell proliferation, and increased cell apoptosis. These findings challenge the traditional notion of E2f4 as a transcription repressor.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Developmental Biology
Stephanie Keer, Helene Cousin, Karyn Jourdeuil, Karen M. Neilson, Andre L. P. Tavares, Dominique Alfandari, Sally A. Moody
Summary: Mcrs1 is crucial for various cellular processes and its loss results in altered gene expression and defects in the development of cranial tissues associated with BOR syndrome.
DEVELOPMENTAL BIOLOGY
(2022)
Article
Biology
Erica J. Hutchins, Shashank Gandhi, Jose Chacon, Michael Piacentino, Marianne E. Bronner
Summary: This study suggests that Elavl1, an RNA-binding protein, plays a critical role in the maintenance of cranial neural crest specification by stabilizing Draxin mRNA. This finding highlights the importance of post-transcriptional regulation in modulating the neural crest specification gene regulatory network.
Article
Biology
Eric Paulissen, Nicholas J. Palmisano, Joshua S. Waxman, Benjamin L. Martin
Summary: This study reveals the critical role of somite morphogenesis in organizing surrounding tissues to facilitate notochord positioning and angioblast migration, ultimately leading to the formation of a functional cardiovascular system in zebrafish embryos.
Article
Multidisciplinary Sciences
Aya Mikdache, Marie-Jose Boueid, Lorijn van der Spek, Emilie Lesport, Brigitte Delespierre, Julien Loisel-Duwattez, Cindy Degerny, Marcel Tawk
Summary: Rgs4 plays a crucial role in neural development, particularly in axon outgrowth and neuronal numbers, by regulating signaling pathways such as Akt, Erk, and mTOR.
SCIENTIFIC REPORTS
(2021)
Article
Endocrinology & Metabolism
Francesca Tonelli, Laura Leoni, Valentina Daponte, Roberta Gioia, Silvia Cotti, Imke A. K. Fiedler, Daria Larianova, Andy Willaert, Paul J. Coucke, Simona Villani, Bjoern Busse, Roberta Besio, Antonio Rossi, P. Eckhard Witten, Antonella Forlino
Summary: This study used zebrafish to investigate the role of TRIC-B in skeletal tissue. The researchers found that the tmem38b gene is expressed at early developmental stages in zebrafish, while tmem38a gene is not. Mutations in tmem38b resulted in under-modified collagen type I and intracellular retention. These findings contribute to our understanding of the importance of TRIC-B in bone cell differentiation.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Engineering, Biomedical
Sung-Min Kim, Daniel Ward Long, Michael Wai Kok Tsang, Yadong Wang
Article
Biochemistry & Molecular Biology
Wes Brown, Jihe Liu, Michael Tsang, Alexander Deiters
Article
Developmental Biology
Maria A. Missinato, Manush Saydmohammed, Daniel A. Zuppo, Krithika S. Rao, Graham W. Opie, Bernhard Kuhn, Michael Tsang
Article
Dentistry, Oral Surgery & Medicine
Erika Calvano Kuchler, Lea Assed da Silva, Paulo Nelson-Filho, Ticiana M. Saboia, Angela M. Rentschler, Jose Mauro Granjeiro, Driely Oliveira, Patricia N. Tannure, Raquel Assed da Silva, Leonardo Santos Antunes, Michael Tsang, Alexandre R. Vieira
JOURNAL OF APPLIED ORAL SCIENCE
(2018)
Article
Biochemistry & Molecular Biology
Manush Saydmohammed, Laura L. Vollmer, Ezenwa O. Onuoha, Taber S. Maskrey, Gregory Gibson, Simon C. Watkins, Peter Wipf, Andreas Vogt, Michael Tsang
Article
Multidisciplinary Sciences
Manush Saydmohammed, Hisato Yagi, Michael Calderon, Madeline J. Clark, Timothy Feinstein, Ming Sun, Donna B. Stolz, Simon C. Watkins, Jeffrey D. Amack, Cecilia W. Lo, Michael Tsang
NATURE COMMUNICATIONS
(2018)
Article
Cell Biology
Lauren Brilli Skvarca, Hwa In Han, Eugenel B. Espiritu, Maria A. Missinato, Elizabeth R. Rochon, Michael D. McDaniels, Abha S. Bais, Beth L. Roman, Joshua S. Waxman, Simon C. Watkins, Alan J. Davidson, Michael Tsang, Neil A. Hukriede
DISEASE MODELS & MECHANISMS
(2019)
Review
Cell Biology
D. A. Zuppo, M. Tsang
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Elizabeth R. Rochon, Maria Azzurra Missinato, Jianmin Xue, Jesus Tejero, Michael Tsang, Mark T. Gladwin, Paola Corti
ANTIOXIDANTS & REDOX SIGNALING
(2020)
Article
Chemistry, Multidisciplinary
Wenyuan Zhou, Wes Brown, Anirban Bardhan, Michael Delaney, Amber S. Ilk, Randy R. Rauen, Shoeb I. Kahn, Michael Tsang, Alexander Deiters
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2020)
Article
Anatomy & Morphology
Maria A. Missinato, Daniel A. Zuppo, Simon C. Watkins, Marcel P. Bruchez, Michael Tsang
Summary: In zebrafish, cardiac regeneration following chemoptogenetic cardiomyocyte ablation does not activate the epicardium and endocardium as seen in other injury models, but still leads to macrophage infiltration and compromised heart function. However, the spared cardiomyocytes undergo proliferation and restore heart structure and function within 45-60 days.
DEVELOPMENTAL DYNAMICS
(2021)
Article
Biochemistry & Molecular Biology
Taylor M. Courtney, Kristie E. Darrah, Trevor J. Horst, Michael Tsang, Alexander Deiters
Summary: A new caged rapamycin analog has been developed with minimal background activity for protein dimerization and easy integration with established FKBP/FRB systems. It has been successfully used for optical control of protein function and signaling pathways.
ACS CHEMICAL BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Kristie Darrah, Joshua Wesalo, Bradley Lukasak, Michael Tsang, James K. Chen, Alexander Deiters
Summary: Conditionally activated caged morpholino antisense agents (cMOs) are tools that allow for investigation of gene expression, regulation, and function during embryonic development. The synthesis of small molecule-responsive cMOs with a flexible linker design enables rapid decaging via Staudinger reduction, leading to gene knockdown in zebrafish embryos. This represents a novel bioorthogonal approach to gene knockdown.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Developmental Biology
Daniel A. Zuppo, Maria A. Missinato, Lucas Santana-Santos, Guang Li, Panayiotis Benos, Michael Tsang
Summary: The poor regenerative capacity of the mammalian heart may be due to the inability of adult cardiomyocytes to proliferate at sufficient levels. However, research has shown that the transcription factor Foxm1 can induce cardiomyocyte proliferation after injury through regulation of cell cycle genes.
Meeting Abstract
Cardiac & Cardiovascular Systems
Elizabeth R. Rochon, Jianmin Xue, Manush Saydmohammed, Cecilia W. Lo, Michael Tsang, Mark T. Gladwin, Paola Corti
