Article
Cell Biology
Ian S. Hitchcock, Maximillian Hafer, Veena Sangkhae, Julie A. Tucker
Summary: Thrombopoietin (TPO) and its receptor MPL play essential roles in platelet production and hematopoietic stem cell maintenance, with dysregulation contributing to hematological disorders; abnormalities in the TPO-MPL axis can lead to bone marrow failure or pathological myeloproliferation; recent resolution of the debate over how TPO binding activates MPL holds potential for better-targeted therapies in blood disorders.
Article
Hematology
Qiuhong Wang, Haocai Chang, Qi Shen, Yonghua Li, Da Xing
Summary: Photobiomodulation therapy (PBMT) prevents chemotherapy-induced thrombocytopenia by increasing platelet production and upregulating hepatic TPO expression. PBMT activates Src protein through reactive oxygen species, leading to the promotion of megakaryocytopoiesis and thrombopoiesis. This research suggests that PBMT is a promising therapeutic strategy for the treatment of CIT.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2021)
Review
Biochemistry & Molecular Biology
Divya M. Teli, Anuradha K. Gajjar
Summary: Elevated glucose level due to β-cell dysfunction is a key marker of Type-II diabetes. GSK-3, an enzyme involved in glycogen metabolism control, has been explored for its potential as a target for diabetes treatment. This review article examines the structural analysis and molecular modeling of GSK-3, as well as advancements in the development of GSK-3 inhibitors as potential therapeutics for Type II diabetes.
BIOORGANIC & MEDICINAL CHEMISTRY
(2023)
Article
Fisheries
Xiaotong Sun, Hongtao Nie, Xiwu Yan
Summary: This study identified and investigated three novel glycogen metabolism-related proteins in Manila clam, suggesting their potential involvement in innate immunity and important role in immune defense.
Review
Biochemistry & Molecular Biology
Chia-Ling Chen, Po-Chun Tseng, Rahmat Dani Satria, Thi Thuy Nguyen, Cheng-Chieh Tsai, Chiou-Feng Lin
Summary: GSK-3 is a vital regulator of glycogen synthesis that plays a crucial role in cellular bioregulation. Abnormal GSK-3 activation and inactivation can lead to liver damage. Targeting GSK-3 with drugs is a potential therapeutic approach for liver protection. Additionally, blocking GSK-3 has a protective effect in IFN-gamma-mediated immune hepatitis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Parisa Badiee, Michelle F. Maritz, Benjamin Thierry
Summary: A nanoformulation of a small molecule GSK3 inhibitor has been developed as a potential alternative to antibody-based checkpoint inhibition. The nanoformulation efficiently inhibits PD-1 expression and improves the survival and proliferation of CAR-T cells, while also increasing the population of memory T cells.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Multidisciplinary Sciences
Long He, Jennifer Endress, Sungyun Cho, Zhongchi Li, Yuxiang Zheng, John M. Asara, John Blenis
Summary: Serine/one-carbon metabolism plays a critical role in cancer cell growth, but the regulatory mechanisms are not well understood. This study reveals that glycogen synthase kinase 3 (GSK3) plays a key role in regulating the expression of serine/one-carbon metabolic enzymes. Inhibition of nuclear GSK3 signaling enhances the efficacy of serine/one-carbon metabolism inhibitors in suppressing cancer cell proliferation.
Review
Cell Biology
Cedric Garcia, Baptiste Compagnon, Michael Poette, Marie-Pierre Gratacap, Francois-Xavier Lapebie, Sophie Voisin, Vincent Minville, Bernard Payrastre, Fanny Vardon-Bounes, Agnes Ribes
Summary: Platelets play a crucial role in hemostasis and thrombosis, but they are also involved in mechanisms associated with inflammation, thrombosis, and the immune system in various diseases. During sepsis, platelets enhance the recruitment and activation of immune cells and contribute to pathogen elimination. However, these mechanisms can also lead to severe organ dysfunction.
Review
Cell Biology
Ryan T. McCallum, Melissa L. Perreault
Summary: This study discussed the widespread multi-system involvement of GSK-3 in contributing to the neuropathology of MDD, emphasizing the feed-forward mechanistic links between all major neuronal signaling pathways.
Article
Biochemistry & Molecular Biology
Chantalle Moolman, Rencia van der Sluis, Richard M. Beteck, Lesetja J. Legoabe
Summary: Plasmodium falciparum glycogen synthase kinase-3 (PfGSK-3) has been identified as a potential target for the development of novel drugs against multi-drug resistant malaria. A series of benzofuran-based compounds were synthesized and evaluated as inhibitors of PfGSK-3 and human glycogen synthase kinase-3 beta (HsGSK-3 beta). Among these compounds, five preferentially inhibited PfGSK-3, with four exhibiting IC50 values in the sub-micromolar range. Evaluation of the structure-activity relationships revealed potential for future design of PfGSK-3 selective inhibitors using chalcone-based scaffolds like benzofurans.
BIOORGANIC CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Veerabahu Subbukutti, Ethirajulu Sailatha, Sethu Gunasekaran, Subramaniyan Manibalan, Kumba Janarthanan Uma Devi, Koteeswaran Bhuvaneshwari, Rajendran Suvedha
Summary: The wound-healing process can be accelerated by inhibiting proteins that slow down the healing pathway. Catnenin, an active protein, is involved in enhancing healing at the nuclear level and in gene expression. A medicated wound dressing transdermal patch with fusion of bio wastes was analyzed against GSK3 beta to enhance healing. The stability and efficiency of the patch in accelerating wound healing process were confirmed through molecular dynamics simulation studies.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
David Cabezas, Guido Mellado, Nicolas Espinoza, Jose Antonio Garate, Cesar Morales, Alejandro Castro-Alvarez, Maria J. Matos, Marco Mellado, Jaime Mella
Summary: Glycogen synthase kinase 3 (GSK-3) plays a role in various diseases and inhibiting GSK-3 could have pro-insulin effects. Aminopyrimidine derivatives have already proven to be promising GSK-3 inhibitors. In this study, CoMFA and CoMSIA models were developed to design new compounds with antidiabetic activity.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Neurosciences
Shaohui Wang, Yao Jiang, Yabo Liu, Qianhui Liu, Hongwei Sun, Mengjie Mei, Xiaomei Liao
Summary: This study demonstrates that ferroptosis promotes abnormal aggregation of tau protein and may serve as a promising therapeutic target for tauopathies.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Biochemical Research Methods
Kevin W. Cormier, Brett Larsen, Anne-Claude Gingras, James R. Woodgett
Summary: The functional differences between the two isoforms of glycogen synthase kinase-3 (GSK-3), a protein-serine/threonine kinase, are still not fully understood. While the isoforms are structurally similar and redundant, there is evidence for specific roles. This study aimed to identify novel GSK-3 interaction partners and compare the interactomes of the two isoforms using affinity purification and proximity-dependent biotinylation mass spectrometry methods. The results showed that the interactomes of the isoforms were highly similar in HEK293 cells, but there were differences in HeLa cells, suggesting isoform-specific associations with certain proteins.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemical Research Methods
Kevin W. Cormier, Brett Larsen, Anne-Claude Gingras, James R. Woodgett
Summary: This study investigates the functional differentiation of two isoforms of GSK-3 and identifies novel interaction partners using affinity purification and proximity-dependent biotinylation mass spectrometry methods. The interactomes of the two isoforms are highly similar in HEK293 cells, but there are isoform-specific interactions in HeLa cells. Understanding the differences in interactions between the isoforms may provide insights into their distinct functions and regulation, and open up possibilities for isoform-specific strategies.
JOURNAL OF PROTEOME RESEARCH
(2023)