Article
Engineering, Biomedical
S. Kotagudda Ranganath, M. Schlund, Jerome Delattre, J. Ferri, F. Chai
Summary: Most existing preclinical models for evaluating the biosafety and bone-regeneration efficacy of bone substitute materials (BSMs) or tissue engineering (TE) constructs have limitations. This study developed a novel double-site critical-sized defect (CSD) model in one rabbit, which reduces the number of animals needed and avoids the influence of individual differences and evaluation bias.
MATERIALS TODAY BIO
(2022)
Article
Chemistry, Physical
Dirk Waehnert, Julian Koettnitz, Madlen Merten, Daniel Kronenberg, Richard Stange, Johannes F. W. Greiner, Christian Kaltschmidt, Thomas Vordemvenne, Barbara Kaltschmidt
Summary: Comparing different bone substitute materials in rat calvarial critical size defects, transplantation of Spongostan(TM) resulted in superior regeneration, indicating its potential as an alternative for defects in the maxillary region.
Article
Engineering, Biomedical
Xueqin Gao, Mintai P. Hwang, Nathaniel Wright, Aiping Lu, Joseph J. Ruzbarsky, Matthieu Huard, Haizi Cheng, Michael Mullen, Sudheer Ravuri, Bing Wang, Yadong Wang, Johnny Huard
Summary: This study compared the effectiveness of 5 bone morphogenetic proteins (BMPs) in promoting bone defect healing using a sustained-release platform. BMP2 and BMP7 were found to have the best bone regeneration capabilities.
Article
Cell & Tissue Engineering
Zoe M. Johnson, Yuan Yuan, Xiangjia Li, Tea Jashashvili, Michael Jamieson, Mark Urata, Yong Chen, Yang Chai
Summary: Craniofacial bones play vital roles in protecting organs and shaping facial features. Critical-size defects in calvarial bones present a major challenge due to their causes and treatment complexities. Utilizing mesenchymal stem cells with a 3D-printed osteoconductive scaffold offers a promising approach for high-quality bone regeneration in calvarial defects.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Ru-Lin Huang, Rao Fu, Yuxin Yan, Chuanqi Liu, Jing Yang, Yun Xie, Qingfeng Li
Summary: Developmental engineering of hypertrophic cartilaginous constructs engineered from adipose tissue can effectively heal large bone defects, exhibiting superior regenerative capacity and tissue integration.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2022)
Article
Engineering, Biomedical
Sanika Suvarnapathaki, Xinchen Wu, Tengfei Zhang, Michelle A. Nguyen, Anastasia A. Goulopoulos, Bin Wu, Gulden Camci-Unal
Summary: Recent innovations in bone tissue engineering have introduced biomaterials that generate oxygen to substitute vasculature. This study demonstrates a novel oxygen-generating tissue scaffold with predictable oxygen release kinetics and modular material properties. The scaffolds showed consistent tissue viability, metabolic activity, and osteogenic differentiation in both in vitro and in vivo experiments.
BIOACTIVE MATERIALS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Tingting Guo, Xiaohong Yuan, Xin Li, Yi Liu, Jian Zhou
Summary: The study aimed to assess the feasibility of using marrow-derived mesenchymal stem cells (BMSCs) cell sheets co-expressing bone morphogenetic proteins 2 (BMP2) and vascular endothelial growth factor (VEGF) for repairing critical-sized calvarial defects. The results showed that treatment with BMP2/VEGF cell sheets significantly promoted bone regeneration in critical-sized calvarial bone defects compared to single-gene transduction or vehicle controls. This study demonstrates that BMSCs cell sheets expressing BMP2/VEGF provide a functional bioactive scaffold for critical-size bone reconstruction.
JOURNAL OF DENTAL SCIENCES
(2023)
Article
Materials Science, Multidisciplinary
Shivaji Kashte, Rohit Dhumal, Pradip Chaudhary, R. K. Sharma, Vikas Dighe, Sachin Kadam
Summary: This study compared the potential of four different scaffolds in healing critical size calvarial bone defects in rat models, with PCL-GO-CQ-hUCMSCs scaffolds exhibiting the highest bone regeneration after 12 weeks. The unique combination of this scaffold proved beneficial in aiding bone regeneration and nearly complete healing of the defect site.
MATERIALS TODAY COMMUNICATIONS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Chengwei Tu, Aisha Bajwa, Andi Shi, Gang Wu, Jingxiao Wang
Summary: The results of this study showed that the combination of deproteinized bovine bone and autologous bone grafts stimulated calvarial bone defect regeneration, while the addition of fibrin glue did not accelerate new bone formation.
CLINICAL ORAL INVESTIGATIONS
(2022)
Article
Chemistry, Physical
Mindaugas Pranskunas, Egidijus Simoliunas, Milda Alksne, Victor Martin, Pedro Sousa Gomes, Algirdas Puisys, Algirdas Kaupinis, Gintaras Juodzbalys
Summary: The study evaluated the functionality of secretome isolated from periosteum-derived MSCs in healing critical size calvarial bone defects in rabbits, showing that osteogenic-induced secretome enhanced new bone formation process and had the highest bone tissue formation. The findings suggest that MSC secretome, particularly from osteogenic-induced populations, may be an effective therapeutic approach to enhance bone tissue healing and regeneration.
Article
Cell Biology
Marie Dubus, Loic Scomazzon, Charlotte Ledouble, Julien Braux, Abdelilah Beljebbar, Laurence Van Gulick, Adrien Baldit, Caroline Gorin, Halima Alem, Nicole Bouland, Marissa Britton, Jessica Schiavi, Ted J. Vaughan, Cedric Mauprivez, Halima Kerdjoudj
Summary: The functionalization of bone-side collagen membrane with dicalcium phosphate dihydrate, chitosan, and hyaluronic acid led to efficient repair of critical-sized bone defects in a rat model, with the formation of new bone exhibiting physiological matrix composition and structural organization. Thorough multi-scale characterization is crucial in assessing biomaterial outcomes in animal models.
Article
Surgery
Malik Hudieb, Adeeb Haddad, Mohammad Bakeer, Ahmad Alkhazaaleh, Mustafa AlKhader, Dafi Taani, Shohei Kasugai
Summary: The study confirmed that a 5mm defect is considered a critical size for calvarial bone defects in young adult rats, while a 4mm defect might be considered critical size for the aged rats after 8 weeks.
JOURNAL OF CRANIOFACIAL SURGERY
(2021)
Article
Chemistry, Multidisciplinary
Jie Zhang, Boyou Zhang, Zefeng Zheng, Qingyun Cai, Jingcheng Wang, Qiang Shu, Lijia Wang
Summary: The study introduces a novel photothermal tissue-engineered bone (PTEB) by seeding mesenchymal stem cells and osteo-induction extracellular matrix in a specific scaffold, promoting bone regeneration under near-infrared radiation. Results demonstrate high biocompatibility and osteo-induction ability of PTEB both in vitro and in vivo.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Polymer Science
Chong Teng, Zhicheng Tong, Qiulin He, Huangrong Zhu, Lu Wang, Xianzhu Zhang, Wei Wei
Summary: In this study, biocompatible hydrogel microspheres were fabricated to promote the osteogenic differentiation of stem cells and enhance bone regeneration. The results from in vivo experiments demonstrated the superiority of the BMSC/HM system in inducing bone regeneration. This study provides a simple and effective research idea for bone defect repair, and the optimization of hydrogel microspheres holds promise for future tissue engineering applications.
Article
Biochemistry & Molecular Biology
Carlos Humberto Valencia-Llano, Diego Lopez-Tenorio, Marcela Saavedra, Paula A. Zapata, Carlos David Grande-Tovar
Summary: Autologous bone is the standard for bone regeneration, but finding alternative materials is still important. This study used bovine xenografts to study critical-size bone defects, and found that these grafts can induce new bone formation, showing potential clinical application in bone regeneration.