期刊
PLASMID
卷 71, 期 -, 页码 16-22出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.plasmid.2014.01.001
关键词
Trichoderma reesei; Alkaline serine protease; Alkaline endoglucanase
资金
- National Basic Research Program of China (973) [2012CB721103]
- National High Technology Research and Development Program of China (863) [2012AA022206]
- Fundamental Research Funds of the Central Universities
The filamentous fungus Trichoderma reesei has received attention as a host for heterologous protein production because of its high secretion capacity and eukaryotic post-translational modifications. However, the heterologous production of proteins in T. reesei is limited by its high expression of proteases. The pH control strategies have been proposed for eliminating acidic, but not alkaline, protease activity. In this study, we verified the expression of a relatively major extracellular alkaline protease (GenBank accession number: EGR49466.1, named spw in this study) from 20 candidates through real-time polymerase chain reaction. The transcriptional level of spw increased about 136 times in response to bovine serum albumin as the sole nitrogen source. Additionally, extracellular protease activity was reduced by deleting the spw gene. Therefore, using this gene expression system, we observed enhanced production and stability of the heterologous alkaline endoglucanase EGV from Humicola insolens using the Delta spw strain as compared to the parental strain RUT-C30. (C) 2014 Elsevier Inc. All rights reserved.
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