期刊
PLASMID
卷 69, 期 2, 页码 172-179出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.plasmid.2012.12.001
关键词
Bacteroides; CTnDOT; Xis2c; Xis2d; Exc; Gene regulation
资金
- National Institute of Health [AI/GM 22383, GA28717]
The Bacteroides conjugative transposon, CTnDOT, is an integrated conjugative element (ICE), found in many human colonic Bacteroides spp. strains. It has a complex regulatory system for both excision from the chromosome and transfer and mobilization into a new host. It was previously shown that a cloned DNA segment encoding the xis2c, xis2d, orf3, and exc genes was required for tetracycline dependent activation of the P-tra promoter. The Xis2c and Xis2d proteins are required for excision while the Exc protein stimulates excision. We report here that neither the Orf3 nor the Exc proteins are involved in activation of the P-tra promoter. Deletion analysis and electromobility shift assays showed that the Xis2c and Xis2d proteins bind to the P-tra promoter to activate the tra operon. Thus, the recombination directionality factors of CTnDOT excision also function as activator proteins of the P-tra promoter. Published by Elsevier Inc.
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