Article
Microbiology
Pierre-Etienne Marcoux, Sarah B. Girard, Kim C. Fournier, Catherine A. Tardif, Ariane Gosselin, Steve J. Charette
Summary: This study reveals the fusion process between the pAsa5 plasmid and the complete version of the pAsa8 plasmid. The insertion of the pAsa8 transposon into pAsa5 in a certain strain appears to be an abnormal event compared to the fusion of the two full-length plasmids. The fusion is mediated by a 22-nucleotide sequence present in both plasmids. Furthermore, the study shows that pAsa8 can be introduced into new strains through conjugation and fusion with pAsa5 can be detected.
Review
Multidisciplinary Sciences
Antony T. Vincent, Nava Hosseini, Steve J. Charette
Summary: This literature review provides an overview of the plasmidome of Aeromonas salmonicida, focusing on antibiotic resistance genes, virulence factors, and cryptic plasmids with unknown biological functions.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2021)
Article
Infectious Diseases
Kim C. Fournier, Valerie E. Paquet, Sabrina A. Attere, Judith Farley, Helene Marquis, Hubert Gantelet, Christian Ravaille, Antony T. Vincent, Steve J. Charette
Summary: New variants of the pRAS3 plasmid were discovered, along with a genetic element similar to one found in a swine pathogen. Despite differences between plasmids, no specific trend in tetracycline resistance was observed. Furthermore, Blast analysis revealed the widespread distribution of pRAS3 plasmids in other bacterial species.
Article
Infectious Diseases
Pierre-Etienne Marcoux, Sabrina A. Attere, Valerie E. Paquet, Maude F. Paquet, Sarah B. Girard, Judith Farley, Michel Frenette, Antony T. Vincent, Steve J. Charette
Summary: We discovered a plasmid in Aeromonas salmonicida subsp. salmonicida, called pAsa-2939, which carries antibiotic resistance genes. This 67 kb plasmid displays unique features and contains a transposon with an unusual organization. The transposon carries a catB3 gene that has not been found in this bacterial species before. The plasmid can be transferred into Aeromonas hydrophila, but not into Escherichia coli.
Article
Biotechnology & Applied Microbiology
Sabrina A. Attere, Cynthia Gagne-Thivierge, Valerie E. Paquet, Gabrielle R. Leduc, Antony T. Vincent, Steve J. Charette
Summary: Except for Aeromonas salmonicida, which includes both psychrophilic and mesophilic subspecies, all 36 known species of the genus Aeromonas are mesophilic. In recent years, an increasing number of mesophilic A. salmonicida strains have been discovered, with only A. salmonicida subsp. pectinolytica being officially classified as a mesophilic subspecies. Most mesophiles have been isolated in hot countries. This study presents the characterization of two new mesophilic isolates from Quebec, Canada, which is the first time mesophilic strains from different countries are found to be so similar.
Article
Virology
Nava Hosseini, Valerie E. Paquet, Mahdi Chehreghani, Sylvain Moineau, Steve J. Charette
Summary: Aquaculture is facing antibiotic resistance issues in fighting pathogenic bacteria, with phage therapy being a potential alternative. A specific combination of four phages has shown effectiveness against A. salmonicida subsp. salmonicida, but strains with Prophage 3 exhibit reduced sensitivity. This highlights the impact of prophage content on the efficacy of phage therapy.
Article
Biochemistry & Molecular Biology
Saqr Abushattal, Ana Vences, Carlos R. Osorio
Summary: The unstable plasmid pPHDPT3, encoding a type III secretion system, is a major virulence factor of the marine bacterium Photobacterium damselae subsp. piscicida (Pdp). However, pPHDPT3 undergoes curing upon in vitro cultivation, resulting in a significant reduction in Pdp's virulence for fish.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Microbiology
Steve J. Charette
Summary: The bacterial species Aeromonas salmonicida is a fish pathogen known for its more diverse characteristics, including different growth temperatures and host ranges. This diversity is partly explained by the presence of numerous mobile genetic elements within the species.
Article
Virology
Nava Hosseini, Valerie E. Paquet, Pierre-Etienne Marcoux, Charles-Antoine Alain, Maude F. Paquet, Sylvain Moineau, Steve J. Charette
Summary: The isolation and characterization of the highly specific phage MQM1 provides a solution for the antibiotic resistance issue in furunculosis caused by A. salmonicida subsp. salmonicida. It may be a valuable addition to future phage cocktails against this fish disease.
Article
Microbiology
Pierre-Etienne Marcoux, Antony T. Vincent, Marie-Ange Massicotte, Valerie E. Paquet, Emilie J. Doucet, Nava Hosseini, Melanie V. Trudel, Gabriel Byatt, Mathilde Laurent, Michel Frenette, Steve J. Charette
Summary: European strains of Aeromonas salmonicida subsp. salmonicida are more prone to losing the TTSS locus compared to Canadian strains. A cluster of genes encoding putative proteins with DNA binding capacity and phage proteins was found to be almost perfectly correlated with the susceptibility of different strains to lose the locus. This discovery opens up new opportunities for studying the thermosensitivity of pAsa5.
Article
Fisheries
Mengmeng Yi, Huanda Lu, Yishuai Du, Guoxiang Sun, Ce Shi, Xian Li, Huiqin Tian, Ying Liu
Summary: This study investigated the influence of Aeromonas salmonicida infection on the body color changes of Atlantic salmon and the levels of cortisol, MSH, and MCH. The results showed that A. salmonicida infection led to a decrease in body color contrast and an increase in cortisol, MSH, and MCH levels.
AQUACULTURE REPORTS
(2021)
Article
Veterinary Sciences
Jiajia Wang, Lijun Xiu, Ying Qiao, Youyu Zhang
Summary: It was previously believed that psychrophilic Aeromonas salmonicida could not infect mammals and humans due to its inability to grow above 25 degrees C. However, a new study found that a specific strain of mesophilic A. salmonicida isolated from Epinephelus coioides was able to cause furunculosis. The researchers discovered that the Zn2+ uptake related genes znuA, znuB, and znuC might play a role in the virulence regulation of A. salmonicida SRW-OG1. Silencing these genes resulted in restricted growth under Fe2+ starvation and decreased motility, biofilm formation, adhesion, and hemolysis. The expression of znuABC was also found to be affected by different growth conditions and stressors.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Diego Rey-Varela, Miguel Balado, Manuel L. Lemos
Summary: Aeromonas salmonicida subsp. salmonicida (A. salmonicida), a Gram-negative bacterium causing furunculosis in fish, produces siderophores acinetobactin and amonabactins to extract iron, but the regulation pathways and conditions for their production are not clear.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Fisheries
Sarah B. Girard, Valerie E. Paquet, Steve J. Charette
Summary: Aeromonas salmonicida subspecies salmonicida, a fish pathogen, expresses various virulence factors such as an A-layer, lipases and proteases during infection. This study optimized protocols for liquid tests to quickly and accurately determine the presence of these virulence factors. The new protocols provide opportunities for quicker characterization and more precise results for A. salmonicida subsp. salmonicida strains.
JOURNAL OF FISH DISEASES
(2022)
Article
Fisheries
Yanyun Liu, Xiaohao Li, Yuqing Xia, Jianxin Cheng, Cheng Zhou, Peng-fei Liu
Summary: This study found that the bacterium A. salmonicida can change the structure and metabolism of gut microbiota in Atlantic salmon, affecting their resistance to infection. The relative abundance of certain gut bacteria significantly increased in the infection groups, which was closely related to differential metabolites. These differential metabolites are involved in important processes such as bile secretion, antibiotic biosynthesis, inflammatory mediator regulation, and tryptophan metabolism.
Article
Biochemistry & Molecular Biology
Marta A. Lages, M. Carmen de la Fuente, Lucia Ageitos, Diana Martinez-Matamoros, Jaime Rodriguez, Miguel Balado, Carlos Jimenez, Manuel L. Lemos
Summary: The study confirmed the importance of FrpA in mediating ferri-Pcb uptake by analyzing mutants lacking FrpA. Additionally, the authors evaluated the internalization ability of several Pcb analogues, and concluded that maintaining a hydroxyl group at position C-13 is crucial for chelating Fe3+ ions.
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Saqr Abushattal, Ana Vences, Carlos R. Osorio
Summary: The unstable plasmid pPHDPT3, encoding a type III secretion system, is a major virulence factor of the marine bacterium Photobacterium damselae subsp. piscicida (Pdp). However, pPHDPT3 undergoes curing upon in vitro cultivation, resulting in a significant reduction in Pdp's virulence for fish.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Javier Cisneros-Sureda, Diego Rey-Varela, Jaime Rodriguez, Miguel Balado, Manuel L. Lemos, Carlos Jimenez
Summary: This study reports the design and synthesis of a new fluorescent probe based on amonabactins, which can label Aeromonas bacteria through their specific transport proteins. The probe also has the potential for selective labeling of other Aeromonas species and can be used for detection of Aeromonas in water and fish farming samples.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Marta A. Lages, Lucia Ageitos, Jaime Rodriguez, Carlos Jimenez, Manuel L. Lemos, Miguel Balado
Summary: This study identified key functions encoded by irp-HPI that are necessary for piscibactin production and transport. Irp4 was found to be an essential thioesterase for piscibactin release, while Irp8 was identified as a crucial protein for piscibactin secretion. FrpBC was shown to be responsible for the internalization of ferri-piscibactin. These findings provide valuable insights for the design of novel antimicrobials against pathogenic vibrios.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Inorganic & Nuclear
M. Carmen de la Fuente, Lucia Ageitos, Marta A. Lages, Diana Martinez-Matamoros, Abel M. Forero, Miguel Balado, Manuel L. Lemos, Jaime Rodriguez, Carlos Jimenez
Summary: This study successfully performed stereo-selective total synthesis of Pcb analogues, which are siderophores produced by pathogenic Gram-negative bacteria. The replacement of the acid-sensitive α-methylthiazoline moiety with a more stable thiazole ring and varying the OH group configuration at C-13 resulted in different metal coordination abilities. The study also confirmed the stereochemistry of the Ga3+ complex and proposed the scaffold's potential use in designing new chelating agents or antibacterials.
INORGANIC CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Diego Rey-Varela, Miguel Balado, Manuel L. Lemos
Summary: Aeromonas salmonicida subsp. salmonicida (A. salmonicida), a Gram-negative bacterium causing furunculosis in fish, produces siderophores acinetobactin and amonabactins to extract iron, but the regulation pathways and conditions for their production are not clear.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
M. Pilar Escribano, Miguel Balado, Alicia E. Toranzo, Manuel L. Lemos, Beatriz Magarinos
Summary: This study analyzed the prevalence of extracellular proteolytic and lipolytic activities related to virulence in Tenacibaculum maritimum strains. They found a great intra-specific heterogeneity in enzymatic capacity, particularly within serotype O4. It was also discovered that OMVs, specifically enriched in certain proteins, played a key role in virulence and biofilm formation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Alba V. Barca, Ana Vences, Mateus S. Terceti, Ana do Vale, Carlos R. Osorio
Summary: This study investigated the transcriptional response of the facultative marine pathogen Photobacterium damselae subsp. damselae to changes in salinity. It was found that low salinity conditions triggered a virulence gene expression profile, while growth at high salinity upregulated genes involved in energy production and metabolism. This study enhances our understanding of the salinity-responsive adaptations of a generalist marine pathogen.
Article
Microbiology
Marta A. Lages, Ana do Vale, Manuel L. Lemos, Miguel Balado
Summary: This study found that PbtA not only interacts with irp-HPI promoters but also regulates the expression of many genes in V. anguillarum, including genes involved in metabolism, immunity, and virulence. These results are significant for understanding the regulatory mechanisms of irp-HPI island and its pathogenicity.
Article
Immunology
A. Rodriguez-Pedrouzo, J. Cisneros-Sureda, D. Martinez-Matamoros, D. Rey-Varela, M. Balado, J. Rodriguez, M. L. Lemos, M. Folgueira, C. Jimenez
Summary: Zebrafish is an excellent model for studying bacterial infections in fish. This study used zebrafish as a model to investigate the infection process of Aeromonas salmonicida in fish and evaluated the use of fluorescence microscopy to track the infection in vivo.
MICROBIAL PATHOGENESIS
(2023)