期刊
PLASMA PROCESSES AND POLYMERS
卷 7, 期 9-10, 页码 794-801出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/ppap.201000020
关键词
contact angle; films; honeycomb structure; mesenchymal stem cell; polycaprolactone; surfaces; surface discharges
资金
- Ministries of Science and Education of Italy and Spain [HI2005-0029]
Polycaprolactone (PCL) scaffolds with a honeycomb (hc) microstructure have been prepared, modified at surface level by plasma processes and evaluated with human mesenchymal stem cells (hMSCs). Thin scaffolds were fabricated by self-assembly of PCL in the presence of an amphiphilic copolymer under controlled humidity. The surface of the so formed PCLhc scaffolds was modified by Ar and Ar-O-2 plasma discharges and by plasma deposition of allylamine (ALA). Such modification induces composition changes and microstructure modifications as determined by Fourier transformed infrared spectroscopy and scanning electron microscopy. As a consequence of these treatments, the hydrophobic character of PCLhc scaffolds is rectified as derived from water contact angle measurements. The hMSC cytocompatibility of these scaffolds was initially assayed under proliferation conditions evaluating surface cell interactions by fluorescence and confocal microscopy. Most relevant behaviour was observed for hMSCs cultured onto ALA modified scaffolds in view of the cytoskeleton polarization. These modified surfaces were further explored for differentiation of hMSCs in the presence of vitamin D, giving rise to secreting vesicles and increased extension of the cytoskeleton. These results are promising for the development of cell monolayer tissue regenerating platforms.
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