Article
Plant Sciences
Xuejie Qu, Shanqun Hu, Tong Li, Jiaqi Zhang, Baoshun Wang, Changli Liu
Summary: In this study, the two varieties of Bupleurum were analyzed for their metabolite composition and clinical efficacy. Significant differences were found in the active components and other pharmacological metabolites between the two varieties, which may contribute to the differences in clinical efficacy. Furthermore, differences in metabolites between the roots and aboveground parts were observed.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Chemistry, Analytical
Hua Zhao, Xiaohui Wang, Yuli Zhang, Xiaowan Huang, Yaling Jiang, Huan Ma, Li An, Xujin Wu, Qiang Wang
Summary: A quantitative proton nuclear magnetic resonance method was developed for the evaluation of saikosaponins in Bupleurum, showing potential as a reliable quality assessment technique.
ANALYTICAL SCIENCES
(2021)
Article
Plant Sciences
Ma Yu, Hua Chen, Qian Liu, Jing Huang, Kassa Semagn, Dan Liu, Yuchan Li, Bin Yang, Yilian He, Chun Sui, Dabin Hou, Jianhe Wei
Summary: By comparing the transcriptome profiles of Bupleurum chinense and B. scorzonerifolium, we identified IAA13 as a gene negatively associated with lateral root number. This study suggests that IAA13 may play a crucial role in regulating lateral root development in these two species.
Article
Pharmacology & Pharmacy
Congcong Chen, Wenxia Gong, Junshen Tian, Xiaoxia Gao, Xuemei Qin, Guanhua Du, Yuzhi Zhou
Summary: This study investigated the effects of Radix Paeoniae Alba (RPA) on Radix Bupleuri (RB)-induced hepatotoxicity. The results showed that RB had an antidepressant effect, but it also caused liver injury. However, RPA effectively reversed RB-induced hepatotoxicity by modulating gut microbiota and promoting the conversion of saikosaponins. It was found that saikosaponin A and saikosaponin D inhibited GSH synthase activity in the liver, leading to liver injury through oxidative stress and the NF-KB/NLRP3 pathway. RPA attenuated the inhibitory effect of saikosaponins on GSS activity, reshaped the liver redox balance, and reversed the liver inflammatory response caused by RB.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2023)
Article
Multidisciplinary Sciences
Ping Wang, Jiqing Bai, Xue Li, Tiantian Liu, Yumeng Yan, Yichang Yang, Huaizhu Li
Summary: In this study, the phylogeny of Chaihu germplasm in China was reconstructed using genome skimming, and potential molecular markers for origin authentication were identified. The results showed that the plastid genomes of Chaihu species were highly conserved, while the noncoding regions had high variability and could serve as promising DNA barcodes for Chaihu authentication. In addition, introgressive hybridization was observed among the Chaihu germplasms. These findings are important for phylogenetic investigation, germplasm authentication, and molecular breeding of Chaihu species.
Article
Plant Sciences
Yuan Kang, Yuan Gao, Ximeng Li, Xinwei Guo, Zhuangzhuang Liu, Wenjing Li, Jianhe Wei, Yun Qi
Summary: This study aims to elucidate the antipyretic characteristics and mechanisms of the traditional Chinese medicine "Bupleuri Radix" (BC). It was found that the water extract of BC can reduce fever in rats and suppress the production of TNF-alpha. Additionally, BC can also inhibit complement activation both in vitro and in vivo. Through these mechanisms, BC may exert its antipyretic effects.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Plant Sciences
Dan-Qi Li, Zi-Wei Zhao, Fan Jiang, Da Liu, Xiao-Jing Wu, Xue-Gui Liu, Pin-Yi Gao
Summary: In this study, researchers identified and characterized 15 triterpenoids from the ethanol extract of Bupleurum scorzonerifolium using various chromatography techniques and spectroscopic methods. Two new compounds were found. These compounds showed significant cytotoxic activities against selected cancer cell lines.
PHYTOCHEMISTRY LETTERS
(2023)
Article
Multidisciplinary Sciences
Hefang Wan, Lei Zhou, Bin Wu, Wenjing Han, Chun Sui, Jianhe Wei
Summary: This study investigated the root metabolome and transcriptome of two cultivars of B. chinense and one of B. scorzonerifolium. The results showed differential metabolites and expression genes between the cultivars, including fatty acyls, organooxygen metabolites, phenolic acids, and lipids. The abundance of flavonoids and saikosaponins varied between the two species, with pinobanksin being the major difference. The expression of chalcone synthase gene was significantly different and correlated with pinobanksin biosynthesis. This study provides a foundation for further research on the biosynthesis of flavonoids and terpenoids in Bupleurum L.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Zhenhuan Wang, Lu Tian, Yusheng Xiao, Mengya Zhao, Yanyan Chang, Yujiang Zhou, Shuying Liu, Huanxi Zhao, Yang Xiu
Summary: Bupleurum chinense DC. and Bupleurum scorzonerifolium Willd. have different clinical efficacies, which are closely related to their complex chemical composition. This study extracted and analyzed the saponins and volatile oils in these two varieties using HPLC and GC-MS, and accurately quantified the absolute contents of five saikosaponins. The results showed significant differences in saikosaponin content between the two varieties, as well as distinct differences in saponins and volatile oils. The study also identified specific compounds contributing to the significant difference between the two varieties.
Article
Biochemistry & Molecular Biology
Wenjing Han, Jiao Xu, Hefang Wan, Lei Zhou, Bin Wu, Jianping Gao, Xinwei Guo, Chun Sui, Jianhe Wei
Summary: BcERF3 is a transcription factor in Chaihu, involved in the biosynthesis of saikosaponins. It is expressed at higher levels in the roots and localized in the nucleus when transiently expressed in tobacco leaves. Overexpression of BcERF3 increases saikosaponin content in Chaihu and upregulates the expression of the key enzyme-encoding gene beta-AS involved in its biosynthesis.
Article
Pharmacology & Pharmacy
Mingming Zhao, Linxuan Xiao, Ke-Gang Linghu, Guanding Zhao, Qiling Chen, Liyu Shen, Parsa Dar, Meiwan Chen, Yuan Hu, Jinming Zhang, Hua Yu
Summary: The anti-inflammatory effects and chemical compositions of Bupleuri chinense DC. (Beichaihu, BCH) and B. scorzonerifolium Willd. (Nanchiahu, NCH) were compared in this study. It was found that NCH exhibited stronger anti-inflammatory effects and had different chemical compositions compared to BCH. Metabolomics and chemometric approaches were used to identify chemical markers for the species discrimination of BCH and NCH.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Wenxi Liu, Xianlong Cheng, Rong Kang, Yadan Wang, Xiaohan Guo, Wenguang Jing, Feng Wei, Shuangcheng Ma
Summary: Saikosaponins are a class of chemical components found in the Bupleurum species with significant biological activities, but their identification is challenging due to structural complexity. This study systematically summarized the identification process of saikosaponins and successfully identified or tentatively characterized 109 saikosaponins from Bupleurum marginatum var. stenophyllum. The study provides important insights for the basic research of Bupleurum species and resource utilization of Radix Bupleuri.
FRONTIERS IN CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Ning Wang, Qian Li
Summary: Saikosaponins from Bupleuri Radix were extracted using ultrasound-assisted extraction and their yields were determined. The antioxidant effect of saikosaponins was investigated and the mechanism was clarified through network pharmacological analysis. The study provided an efficient method for the extraction of saikosaponins and a theoretical basis for their rational utilization and medicinal value.
Article
Biochemical Research Methods
Jiawei Shang, Jianxin Wang, Pengfei Yan, Chengye Yan, Jiaxi Li, Jiahao Li, Xin Yong, Qiao Wang, Xue Xiong, Huijun Xu
Summary: A strategy for extensive metabolite identification in Radix Bupleuri (RB) was proposed to distinguish four commercially available RB species. This strategy includes spectral library search and molecular network analysis, deducing possible structures based on characteristic fragment ions, matching information with reported RB components, and identifying chemical markers using chemometrics. This study successfully identified and distinguished different components of RB and laid a good theoretical foundation for regulating the RB market.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biology
Yanping Mao, Yuping Yang, Yuchan Li, Yiguan Zhang, Ping Wei, Hua Chen, Dabin Hou
Summary: In this study, it was revealed that methyl jasmonate (MeJA) significantly increased the accumulation of saikosaponins (SSs) in Bupleurum chinense DC. roots. A comparative transcriptome analysis identified 104,057 unigenes, of which 4053 were differentially expressed genes (DEGs). The majority of DEGs showed downregulated expression after MeJA treatment, and functional annotation indicated their involvement in stress responses and development. Furthermore, 88 DEGs encoding enzymes in the SS synthesis pathway and 99 DEGs encoding transcription factors (TFs) with different expression patterns were identified. Integrative analysis revealed that 20 DEGs involved in the SS synthesis pathway and 12 DEGs encoding TFs exhibited strong correlations with SS contents, suggesting their importance in SS biosynthesis and regulation.
Article
Plant Sciences
Martine Huberty, Katja Steinauer, Robin Heinen, Renske Jongen, S. Emilia Hannula, Young Hae Choi, T. Martijn Bezemer
Summary: This study investigates the influence of time of conditioning on soil microbiome composition, plant growth, and metabolomics, and plant-insect interactions. The results show that the biomass response of the test plants to soil conditioning remained consistent throughout the year, even though both the soil microbiome and leaf metabolomic responses to conditioned soil varied greatly over time. These soil-induced changes in the metabolome of plants over time can be an important driver of above-ground multitrophic interactions in nature.
JOURNAL OF ECOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Maciej Strzemski, Slawomir Dresler, Beata Podkoscielna, Kamil Skic, Ireneusz Sowa, Daniel Zaluski, Rob Verpoorte, Sylwia Zielinska, Pawel Krawczyk, Magdalena Wojciak
Summary: This study evaluated the potential of using volatile natural deep eutectic solvents (VNADESs) for the efficient and environmentally friendly extraction of Chelidonium alkaloids. The results showed that the use of appropriate VNADESs formulations led to higher extraction yields compared to traditional methods.
Article
Plant Sciences
Orchidea Rachmaniah, Erica G. Wilson, Young Hae Choi, Geert-Jan Witkamp, Robert Verpoorte
Summary: This study developed a natural deep eutectic solvent-based extraction method for galanthamine and found that some acidic and sugar mixtures showed the best results. The natural deep eutectic solvent was more selective for galanthamine compared to methanol extraction.
Review
Multidisciplinary Sciences
Novi Safriani, Fransisca Rungkat Zakaria, Endang Prangdimurti, Suwarti, Robert Verpoorte, Nancy Dewi Yuliana
Summary: Many edible plants have been found to exhibit immunomodulatory activities, but the bioactive compounds responsible for these effects have not been identified. Metabolomics offers a key tool for identifying these compounds in plant extracts. However, its application to the study of immunomodulatory effects of food plants is still underdeveloped.
Article
Biochemistry & Molecular Biology
Nunzio Del Gaudio, Antonella Di Costanzo, Ning Qing Liu, Lidio Conte, Carmela Dell'Aversana, Guglielmo Bove, Rosaria Benedetti, Liliana Montella, Fortunato Ciardiello, Vincenzo Carafa, Concetta Ambrosino, Valeria Tucci, Mariarosaria Conte, Joost H. A. Martens, Hendrik G. Stunnenberg, Angela Nebbioso, Lucia Altucci
Summary: CBX2 plays a crucial role in leukemia progression, and its silencing can inhibit cell proliferation and induce apoptosis, as well as alter cell transcriptional programs. CBX2 suppression also increases chromatin accessibility, alters gene expression, and results in enrichment of cell death pathways and downregulation of survival genes. CBX2 silencing also induces epigenetic reprogramming at p38 MAPK-associated regulatory sites, leading to dysregulation of gene expression.
Article
Biochemistry & Molecular Biology
Tom van Schaik, Stefano G. Manzo, Athanasios E. Vouzas, Ning Qing Liu, Hans Teunissen, Elzo de Wit, David M. Gilbert, Bas van Steensel
Summary: Through studying human cell lines, researchers have discovered that Ki-67 is associated with large genomic domains, particularly those involved in late DNA replication. The distribution pattern of Ki-67 on chromosomes varies throughout different stages of the cell cycle. These findings highlight the important roles that Ki-67 plays in genome interactions and heterochromatin organization.
Article
Geriatrics & Gerontology
Xiaojie Liu, Xiaoling Wu, Di Zhao, Sijun Zhao, Zhenyu Li, Xuemei Qin
Summary: This study found a close relationship between gut microbiota and aging as well as constipation, possibly through metabolism. The composition of gut microbiome was similar in both aging and constipation, and key gut bacteria were clustered together. Gut microbial metabolites played important roles as signaling molecules in aging and constipation, and the occurrence and development of both conditions were influenced by multiple factors.
EXPERIMENTAL GERONTOLOGY
(2022)
Article
Cell Biology
Thom M. Molenaar, Muddassir Malik, Joana Silva, Ning Qing Liu, Judith H. Haarhuis, Christina Ambrosi, Eliza Mari Kwesi-Maliepaard, Tibor van Welsem, Tuncay Baubec, William J. Faller, Fred van Leeuwen
Summary: Cell size is tightly regulated and changes in cell size are frequently observed in cancer. In this study, we found that SETD2, a lysine methyltransferase protein, plays a role in regulating cell size by negatively regulating global protein synthesis rates. Overexpression of the H3K36 demethylase KDM4A or the oncohistone H3.3K36M also led to an increase in cell size, suggesting the importance of H3K36 methylation in cell size regulation.
JOURNAL OF CELL SCIENCE
(2022)
Review
Plant Sciences
Cristiano S. Funari, Daniel Rinaldo, Vanderlan S. Bolzani, Robert Verpoorte
Summary: This review article investigates the response of phytochemists to green chemistry insights since the launch of the Pollution Prevention Act in 1990. Highly cited phytochemistry papers from each year between 1990 and 2019 were sampled to assess the greenness of experimental procedures, particularly regarding the use of solvents. Progress in sustainability was observed in the extraction stage, but not in purification procedures, where hazardous solvents like chloroform and dichloromethane were still commonly used. Potential alternative approaches for more sustainable purification procedures are discussed, along with initiatives for a broader sustainable phytochemical research.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Biochemistry & Molecular Biology
Purin Candra Purnama, Leonardo Castellanos Hernandez, Robert Verpoorte
Summary: The presence and significance of endophytes in plant biosynthetic pathways is still unclear. Fungicide treatments have varying effects on plants, such as decreasing taxol content in Taxus and causing the disappearance of ergot alkaloids in Ipomoea asarifolia. Fungicides also affect the primary and secondary metabolism of plants, as observed in Narcissus pseudonarcissus cv. Carlton and Jacobaea.
Article
Geriatrics & Gerontology
Xiaojie Liu, Ziyu Zhao, Yuhui Fan, Di Zhao, Yaze Wang, Meng Lv, Xuemei Qin
Summary: This study aims to reveal the possible correlations between depression and constipation from the perspectives of gut microbiome and plasma metabolome. Through constructing depressed and constipated rat models, locomotor activity and gastrointestinal functions were measured. Plasma metabolomics and gut microbiota sequencing were used to identify shared and unique metabolites between depression and constipation. The findings show that depression and constipation significantly affect locomotor activity, gastrointestinal functions, and gut microbiota compositions of rats, with perturbations in energy metabolism and amino acid metabolism.
Article
Plant Sciences
Muhammad Jahangir, Atta-ur-Rehman, Ibrahim Bayoumi Abdel Farid, Robert Verpoorte, Imran Khan, Jiangnan Peng
Summary: Adhatoda vasica is a widely distributed plant in Asia used in traditional medicine to treat various infections and health disorders. This study identified a range of compounds, including fatty acids, amino acids, sugars, and flavonoids, using NMR spectroscopy-based metabolomics. Analysis of leaves from different regions showed significant variability in metabolite composition, with geographical factors influencing the profiles.
Article
Plant Sciences
Xiaojie Liu, Xiaoling Wu, Senyan Wang, Xuemei Qin
Summary: This study aimed to reveal the compatibility of Xiaoyaosan (XYS) in anti-depression through the perspective of the gut-liver-kidney axis. Results showed that XYS and its efficacy groups significantly regulated the abnormalities of behaviors, kidney metabolism, and intestinal disorders in depressed rats. XYS exhibited the strongest regulation, followed by the Shugan (SG) and Jianpi (JP) groups. The findings provide innovative insights into the anti-depression effects and compatibility of XYS, as well as a new perspective and methodology for studying complex diseases and traditional Chinese medicine (TCM) compatibility.
Article
Biotechnology & Applied Microbiology
Tom van Schaik, Ning Qing Liu, Stefano G. Manzo, Daan Peric-Hupkes, Elzo de Wit, Bas van Steensel
Summary: CTCF and cohesin are not primary determinants of LAD patterns, but rather locally modulate nuclear lamina interactions.
Article
Biochemistry & Molecular Biology
Xiaojie Liu, Chen Jian, Mengyu Li, Fuxiao Wei, Huanle Liu, Xuemei Qin
Summary: This study investigated the impact of constipation in an aged rat model and the effects of Cistanche deserticola polysaccharide (CDPS) on constipation. The study found that CDPS significantly regulated behavioral indexes, gut microbiota, and fecal metabolites in constipated rats. It increased beneficial bacteria and decreased potentially pathogenic bacteria. 16 potential biomarkers of constipation in aged rats were identified. The findings deepen our understanding of constipation in the elderly and provide a foundation for using polysaccharides to treat constipation and improve the quality of life of the elderly.
