Review
Plant Sciences
Gary P. Munkvold, Robert H. Proctor, Antonio Moretti
Summary: Fusarium is a significant genus of plant-pathogenic fungi and one of the most important mycotoxin-producing genera in the world. Understanding the mycotoxin potential of individual species is crucial for assessing toxicological risks, but efforts to summarize mycotoxin production have been complicated by various classification systems.
ANNUAL REVIEW OF PHYTOPATHOLOGY, VOL 59, 2021
(2021)
Article
Plant Sciences
Hosung Jeon, Jung-Eun Kim, Jung-Wook Yang, Hokyoung Son, Kyunghun Min
Summary: Plant pathogenic fungi cause significant losses in crop yield and mycotoxin contamination. The conventional method of DNA extraction for fungal identification is time-consuming. This study presents a direct PCR approach that bypasses the DNA extraction steps and simplifies the molecular identification of fungal species.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Food Science & Technology
Qinggang Guo, Shixin Li, Lihong Dong, Zhenhe Su, Peipei Wang, Xiaomeng Liu, Ping Ma
Summary: This study established a screening method for biocontrol agents against Fusarium wilt based on tolerance to Fusaric acid and antagonism against F. oxysporum. Three promising biocontrol bacteria were obtained, named B31, F68, and 30833, which successfully controlled tomato, watermelon, and cucumber Fusarium wilt. Phylogenetic analysis confirmed these strains as B. velezensis. Further experiments showed that strains B31, F68, and 30833 exhibited significantly greater tolerance to Fusaric acid compared to strain FZB42.
Article
Food Science & Technology
Ting Dong, Shouning Qiao, Jianhong Xu, Jianrong Shi, Jianbo Qiu, Guizhen Ma
Summary: The study examined how interacting conditions, such as water activity, temperature, and incubation time, affect the growth rate, toxin production, and expression of biosynthetic genes in Fusarium fujikuroi species complex strains. The results showed that high temperature and water availability promoted fungal growth and increased toxin accumulation. The study also identified specific genes associated with the production of certain mycotoxins. This research provides valuable information for monitoring and preventing the contamination of maize with these toxins.
Article
Plant Sciences
Yixue Bao, Sehrish Akbar, Wei Yao, Yuzhi Xu, Jianlong Xu, Charles A. Powell, Baoshan Chen, Muqing Zhang
Summary: This study investigated the diversity of Fusarium spp. associated with PBD in China. Through the collection of 320 leaf samples over 10 years from six sugarcane-growing regions, four FFSC species were identified: F. sacchari, F. proliferatum, F. verticillioides, and F. andiyazi. The results contribute to accurately identifying Fusarium spp. associated with the sugarcane crop.
Article
Biochemistry & Molecular Biology
Giha Song, Seungmin Son, Suhyeon Nam, Eun-Jung Suh, Soo In Lee, Sang Ryeol Park
Summary: Invasive pathogens cause significant damage to crops annually, and identifying genes that confer broad-spectrum resistance is crucial for plant breeding. This study reveals that OsWRKY114 enhances innate immunity in rice against the fungal pathogen Fusarium fujikuroi, in addition to its previously known role against the bacterial pathovar Xanthomonas oryzae pv. oryzae. Overexpression of OsWRKY114 in rice reduces the expression of susceptibility-related genes and increases resistance against F. fujikuroi.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Multidisciplinary
Syafiqa Pramunadipta, Ani Widiastuti, Arif Wibowo, Haruhisa Suga, Achmadi Priyatmojo
Summary: The Fusarium incarnatum-equiseti species complex (FIESC) and Fusarium fujikuroi species complex (FFSC) are two complexes consisting of multiple species. Two primer pairs were identified to be able to identify specific species within these complexes, and different restriction enzymes were used to further differentiate the species. Accurate identification of these pathogens is crucial for disease control in the field.
PLANT PATHOLOGY JOURNAL
(2022)
Article
Microbiology
Claudette Dewing, Magrieta A. Van der Nest, Quentin C. Santana, Robert H. Proctor, Brenda D. Wingfield, Emma T. Steenkamp, Lieschen De Vos
Summary: This study used comparative genomics to elucidate the mechanisms underlying the ability of grass-associated and pine-associated Fusarium species to colonize their respective plant hosts. The results showed that the genes associated with host range originated and were retained through various mechanisms during the evolution of Fusarium. These host-range-associated genes tended to be located towards the subtelomeric regions of chromosomes.
Article
Food Science & Technology
Ana Belen Corallo, Agustina del Palacio, Maria Oliver, Susana Tiscornia, Macarena Simoens, Jaqueline Cea, Ines de Aurrecoechea, Ines Martinez, Alicia Sanchez, Silvina Stewart, Dinorah Pan
Summary: This study found that grain mold and stalk rot in sorghum are caused by different Fusarium spp., resulting in significant yield losses and mycotoxin contamination, posing a risk to consumers.
Article
Materials Science, Multidisciplinary
Yaling Lin, Xixiang Feng, Wei Zhang, Rui Li, Anqiang Zhang
Summary: In order to effectively detect and determine banana Fusarium wilt caused by Foc4, researchers designed MIPCFA based on fusaric acid and MIPC technology. This method can quickly detect FA in minute scale, and can be used for detection in Foc4-containing medium and banana plants, making it an effective and fast method for field application.
ACS APPLIED POLYMER MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
Haonan Wang, Xia Ke, Rui Jia, Lianggang Huang, Zhiqiang Liu, Yuguo Zheng
Summary: In this study, a high-yield GA(3) producing strain of Fusarium fujikuroi was constructed using metabolic modification, transcriptome analysis, and promoter engineering. Over-expression of AreA and Lae1 genes resulted in an initial strain with GA(3) production of 2.78 g L-1. By employing a nitrogen-responsive bidirectional promoter, the production of GA(3) was further increased to 3.02 g L-1. The top 20 upregulated genes in GA(3) over-production were characterized, providing potential targets for further strain development. This work offers an efficient microbial development platform for industrial GA(3) production.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yu-Ke Cen, Ming-han Li, Qi Wang, Jia-Meng Zhang, Jia-Chen Yuan, Yuan-Shan Wang, Zhi-Qiang Liu, Yuguo Zheng
Summary: The industrially applied F. fujikuroi strains are mainly obtained through spontaneous mutagenesis, but their yield is still low. We designed an efficient strain improvement strategy by amphotericin B resistance prescreening and evolutionary engineering, and obtained a strain with a 25% increase in yield and high genetic stability for GAs production.
PROCESS BIOCHEMISTRY
(2023)
Article
Agricultural Engineering
Hao-Nan Wang, Xia Ke, Rui Jia, Liang-Gang Huang, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: In this study, multivariate modular metabolic engineering (MMME) was used to reconstitute the metabolic balance in Fusarium fujikuroi for enhanced production of gibberellic acid (GA(3)). By optimizing three modules including the precursor pool, cluster-specific channel, and P450-mediated oxidation, the production of GA(3) was successfully improved. This study presents a promising approach for efficient microbial transformation in agro-industrial applications.
BIORESOURCE TECHNOLOGY
(2022)
Article
Plant Sciences
Gandhi Kannan, Marimuthu Somasundaram Saraswathi, Raman Thangavelu, Parasuraman Subesh Kumar, Murugesan Bathrinath, Subbaraya Uma, Suthanthiram Backiyarani, Arumugam Chandrasekar, Thumballi R. Ganapathi
Summary: Induced mutagenesis combined with toxin based screening is an effective method to create Fusarium wilt resistant mutants in banana. Global proteomics revealed the molecular mechanism behind resistance.
Article
Plant Sciences
Cui Yang, Jing Sun, Zhaoqun Wu, Maozhu Jiang, Dayong Li, Xinjie Wang, Chunxiang Zhou, Xuecheng Liu, Zhiguo Ren, Jun Wang, Manli Sun, Wenxian Sun, Jie Gao
Summary: In this study, the FoRSR1 gene encoding a Ras-related small GTPase in F. oxysporum f. sp. ginseng was identified and characterized. Disruption of FoRSR1 affected mycelial dry weight, hyphal morphology, conidiation, chlamydospore formation, and virulence in ginseng roots. Deletion of FoRSR1 also had impacts on pigmentation, autophagy, and secondary metabolism. The study demonstrated the important roles of FoRSR1 in various biological processes and pathogenesis in F. oxysporum f. sp. ginseng.
Article
Chemistry, Multidisciplinary
Trong D. Tran, Laura K. Cartner, Heidi R. Bokesch, Curtis J. Henrich, Xin W. Wang, Chulabhorn Mahidol, Somsak Ruchirawat, Prasat Kittakoop, Barry R. O'Keefe, Kirk R. Gustafson
Summary: This study presents two novel alkaloids named damirines A and B from a marine sponge, which feature a unique carbon-nitrogen framework. One of the compounds demonstrated selective cytotoxic properties towards six different cell lines in the NCI-60 cancer screen.
MAGNETIC RESONANCE IN CHEMISTRY
(2021)
Article
Chemistry, Organic
Tanja Grkovic, Somsak Ruchirawat, Prasat Kittakoop, Paul G. Grothaus, Jason R. Evans, John R. Britt, David J. Newman, Chulabhorn Mahidol, Barry R. O'Keefe
Summary: In this study, two bispyrroloiminoquinone alkaloids, wakayin and a new natural product 16-hydroxy-17-oxindole wakayin, were isolated from a Thai ascidian Clavelina sp. The new natural product was structurally elucidated, and its cytotoxic activity was compared to known analogue wakayin and a series of other related pyrroloiminoquinones, suggesting potential medicinal value.
ASIAN JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Sanya Sureram, Irene Arduino, Reiko Ueoka, Massimo Ritta, Rachele Francese, Rattanaporn Srivibool, Dhanushka Darshana, Jorn Piel, Somsak Ruchirawat, Luisa Muratori, David Lembo, Prasat Kittakoop, Manuela Donalisio
Summary: The peptide A-3302-B isolated from a marine bacterium, Micromonospora sp., strain MAG 9-7, showed specific inhibitory activity against HSV-2 by reducing cell-to-cell virus spread and transmission of extracellular free virus. It did not affect viral protein expression. The peptide also demonstrated anti-inflammatory activity and efficacy against an acyclovir-resistant HSV-2 strain.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Nurul Q. Fadilah, Akanitt Jittmittraphap, Pornsawan Leaungwutiwong, Patcharee Pripdeevech, Darshana Dhanushka, Chulabhorn Mahidol, Somsak Ruchirawat, Prasat Kittakoop
Summary: This study explored the composition of a crude extract of Citrus x aurantium peels and found that limonene was the major compound. The essential oil of C. x aurantium peels exhibited antiviral activity against influenza A virus, especially D- and L-limonene. This work demonstrated the antiviral activity of L-limonene for the first time and suggested that D- or L-limonene at certain concentrations could be used as potential disinfectants to prevent airborne transmission of viruses.
NATURAL PRODUCT COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Chomphunuch Songsiriritthigul, Chayanin Hanwarinroj, Bongkochawan Pakamwong, Potjanee Srimanote, Nitima Suttipanta, Sanya Sureram, Khomson Suttisintong, Pharit Kamsri, Auradee Punkvang, James Spencer, Prasat Kittakoop, Pornpan Pungpo
Summary: The study demonstrates that 3-nitropropanoic acid (3NP) inhibits the 2-trans-enoyl-acyl carrier protein reductase (InhA) from Mycobacterium tuberculosis with an IC50 value of 71 μM. The crystal structure of the ternary InhA-NAD(+)-3NP complex reveals the binding mode of 3NP, providing opportunities for improving inhibitory activity towards InhA. The identified interactions between 3NP and InhA substrate-binding loop suggest potential for optimizing inhibitor design.
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2022)
Article
Chemistry, Multidisciplinary
Anuchit Phanumartwiwath, Chatchai Kesornpun, Sanya Sureram, Poonpilas Hongmanee, Pornpan Pungpo, Pharit Kamsri, Auradee Punkvang, Chatchakorn Eurtivong, Prasat Kittakoop, Somsak Ruchirawat
Summary: The isoxazoline derivatives of natural products were evaluated for their antitubercular and antibacterial activities, showing strong inhibitory effects against Bacillus cereus and Mycobacterium tuberculosis. Molecular modelling predicted interactions between the isoxazolines and specific binding sites.
JOURNAL OF CHEMICAL RESEARCH
(2021)
Article
Food Science & Technology
Maharani A. Astiti, Akanitt Jittmittraphap, Pornsawan Leaungwutiwong, Nopporn Chutiwitoonchai, Patcharee Pripdeevech, Chulabhorn Mahidol, Somsak Ruchirawat, Prasat Kittakoop
Summary: A novel method was employed to isolate α-glucosidase inhibitors from C. grandis leaves, resulting in the identification of seven flavonoid glycosides with remarkable inhibitory activity. These compounds also showed significant virucidal activity against influenza A virus H1N1, being 4.4-10.3 times more potent than acarbose.
Article
Chemistry, Medicinal
Chayanin Hanwarinroj, Nareudon Phusi, Bundit Kamsri, Pharit Kamsri, Auradee Punkvang, Sombat Ketrat, Patchreenart Saparpakorn, Supa Hannongbua, Khomson Suttisintong, Prasat Kittakoop, James Spencer, Adrian J. Mulholland, Pornpan Pungpo
Summary: This study used in silico screening approaches to discover four novel InhA inhibitors with potential activity against Mycobacterium tuberculosis. The binding interactions and binding energy of the candidate compounds were investigated using molecular mechanics calculations. These compounds showed suitable physicochemical, pharmacokinetic, and antibacterial properties, making them promising hit compounds for further experimental studies.
FUTURE MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Medicinal
Bongkochawan Pakamwong, Paptawan Thongdee, Bundit Kamsri, Naruedon Phusi, Pharit Kamsri, Auradee Punkvang, Sombat Ketrat, Patchreenart Saparpakorn, Supa Hannongbua, Kanchiyaphat Ariyachaokun, Khomson Suttisintong, Sanya Sureram, Prasat Kittakoop, Poonpilas Hongmanee, Pitak Santanirand, James Spencer, Adrian J. Mulholland, Pompan Pungpo
Summary: This study identified two potential inhibitors of M. tuberculosis DNA gyrase ATPase activity through virtual screening and subsequent biological assays. These compounds showed good antitubercular activity and likely exert their effects by binding to DNA gyrase. Molecular dynamics simulations and pharmacophore mapping analysis revealed the interaction mechanism of these compounds with the target enzyme, providing valuable insights for optimizing antitubercular agents.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2022)
Article
Biochemical Research Methods
Chayanin Hanwarinroj, Paptawan Thongdee, Darunee Sukchit, Somjintana Taveepanich, Pharit Kamsri, Auradee Punkvang, Sombat Ketrat, Patchreenart Saparpakorn, Supa Hannongbua, Khomson Suttisintong, Prasat Kittakoop, James Spencer, Adrian J. Mulholland, Pornpan Pungpo
Summary: This study used quantitative structure-activity relationship methods to investigate the inhibitory effects of quinazoline derivatives on PknB. Molecular dynamics simulations and binding energy calculations revealed that the quinazoline core and overall hydrophobicity were major contributors to the affinity of PknB. Additional quinazoline derivatives were designed and evaluated, and predictive models identified sixteen compounds with superior PknB binding and other desirable properties.
JOURNAL OF MOLECULAR GRAPHICS & MODELLING
(2022)
Article
Chemistry, Medicinal
Paptawan Thongdee, Chayanin Hanwarinroj, Bongkochawan Pakamwong, Pharit Kamsri, Auradee Punkvang, Jiraporn Leanpolchareanchai, Sombat Ketrat, Patchreenart Saparpakorn, Supa Hannongbua, Kanchiyaphat Ariyachaokun, Khomson Suttisintong, Sanya Sureram, Prasat Kittakoop, Poonpilas Hongmanee, Pitak Santanirand, Galina Mukamolova, Rosemary A. Blood, Yuiko Takebayashi, James Spencer, Adrian J. Mulholland, Pornpan Pungpo
Summary: In this study, virtual screening and biological validation were used to identify candidate inhibitors of Mycobacterium tuberculosis protein kinase B (PknB). Three indole compounds were found to inhibit the growth of M. tuberculosis with minimal inhibitory concentrations. Two compounds also showed inhibition of PknB activity in vitro, while another compound exhibited anti-tuberculosis activity without inhibiting PknB. Molecular dynamics simulations were used to analyze the binding of compounds to PknB, confirming their affinity. These findings provide starting points for the development of new anti-tubercular agents.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2022)
Article
Biochemical Research Methods
Narumol Jariyasopit, Suphitcha Limjiasahapong, Alongkorn Kurilung, Sitanan Sartyoungkul, Pattipong Wisanpitayakorn, Narong Nuntasaen, Chutima Kuhakarn, Vichai Reutrakul, Prasat Kittakoop, Yongyut Sirivatanauksorn, Sakda Khoomrung
Summary: The combination of ion mobility mass spectrometry and chromatography is a powerful tool for identifying compounds in natural products. In this study, a comprehensive database for plant specialized metabolites was established and validated using an ultra-performance liquid chromatography system coupled with a high-resolution quadrupole/traveling wave ion mobility spectrometry/time-of-flight mass spectrometry. The database included both commercially available compounds and those that were isolated and purified in-house, providing accurate information on molecular weight, retention times, fragment ions, and ion mobility-derived collision cross section values. The database was successfully applied to extend the metabolite coverage of Ventilago harmandiana Pierre.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Wantanwa Krongrawa, Sontaya Limmatvapirat, Mont Kumpugdee Vollrath, Prasat Kittakoop, Supachai Saibua, Chutima Limmatvapirat
Summary: This study aimed to develop a Kaempferia parviflora (KP) extract-based delivery system using electrospun shellac fibers. The optimal parameters for fiber diameter and bead-to-fiber ratio were determined using a Box-Behnken design. The optimized fibers had a small diameter (574 nm) and a lower bead-to-fiber ratio (0.48 beads per fiber) by combining shellac and KP extract at specific concentrations, solution feed rate, and electrical voltage. The KP extract was dispersed evenly in the electrospun shellac fibers, and in vitro dissolution tests showed sustained release within 10 hours. The KP-loaded shellac fibers also demonstrated antibacterial activity.
Article
Multidisciplinary Sciences
Pornsuda Chawengrum, Natthaorn Luepongpatthana, Sanit Thongnest, Jitnapa Sirirak, Jutatip Boonsombat, Kriengsak Lirdprapamongkol, Siriporn Keeratichamroen, Patcharin Kongwaen, Phreeranat Montatip, Prasat Kittakoop, Jisnuson Svasti, Somsak Ruchirawat
Summary: Anticopalic acid (ACP), together with its derivatives, showed cytotoxic activity against cancer cell lines. Compound 4p exhibited the highest activity against leukemia cell lines, while compound 4l showed activity against triple-negative breast cancer cell line MDA-MB-231. Mechanistic studies revealed that compound 4l induced cell death through non-apoptotic regulated cell death and decreased the phosphorylation of FAK protein.
SCIENTIFIC REPORTS
(2023)
Article
Food Science & Technology
Dhanushka Darshana, Nopporn Chutiwitoonchai, Atchara Paemanee, Sanya Sureram, Chulabhorn Mahidol, Somsak Ruchirawat, Prasat Kittakoop
Summary: This study explored the bioactive compounds present in avocado fruit pulp and identified polyhydroxylated fatty alcohols with potential antiviral activities. Several polyols exhibited virucidal and antiviral activities against a surrogate of SARS-CoV-2, with avocadinin, avocadenol D, and avoenin showing the strongest antiviral effects. Avocado fruit pulp has the potential to be used in the development of antiviral agents.
ACS FOOD SCIENCE & TECHNOLOGY
(2023)
