期刊
PLANTA MEDICA
卷 76, 期 15, 页码 1784-1786出版社
GEORG THIEME VERLAG KG
DOI: 10.1055/s-0030-1249806
关键词
DNA barcoding; species identification; Euphorbiaceae; intra-/interspecific genetic divergence
资金
- Ministry of Science and Technology of China [2007DFA30990]
- Ministry of Health [200802043]
In this study, we tested the applicability of four DNA regions (rbcL, matK, ITS, and ITS2) as barcodes for identifying species within Euphorbiaceae. Based on assessments of the specific genetic divergence, the DNA barcoding gap, and the ability for species discrimination, the present results affirmed that ITS/ITS2 is a potential barcode for the Euphorbiaceae species. This study also provided a large-scale test to evaluate the effectiveness of ITS/ITS2 for differentiating species within Euphorbiaceae. Of the 1183 plant samples collected from 871 species in 66 diverse genera, ITS/ITS2 successfully identified >90% and 100% of them at the species and genus levels, respectively. Therefore, our research indicates that use of the ITS/ITS2 region is a powerful technique for Euphorbiaceae identification.
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