期刊
PLANTA
卷 238, 期 5, 页码 937-953出版社
SPRINGER
DOI: 10.1007/s00425-013-1944-7
关键词
Banana; bHLH; Chilling tolerance; ICE; Protein-protein interaction
资金
- Department of Education of Guangdong Province [2012CXZD0014]
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University [KSL-CUSAb-2012-08]
- National Natural Science Foundation of China [30972068]
- National Public Benefit of Agricultural Research Foundation of China [200903044-5]
- China Agriculture Research System [CARS-32-02A]
Basic helix-loop-helix (bHLH) transcription factors (TFs) are ubiquitously involved in the response of higher plants to various abiotic stresses. However, little is known about bHLH TFs involved in the cold stress response in economically important fruits. Here, five novel full-length bHLH genes, designated as MabHLH1-MabHLH5, were isolated and characterized from banana fruit. Gene expression profiles revealed that MabHLH1/2/4 were induced by cold stress and methyl jasmonate (MeJA) treatment. Transient assays in tobacco BY2 protoplasts showed that MabHLH1/2/4 promoters were activated by cold stress and MeJA treatments. Moreover, protein-protein interaction analysis demonstrated that MabHLH1/2/4 not only physically interacted with each other to form hetero-dimers in the nucleus, but also interacted with an important upstream component of cold signaling MaICE1, with different interaction domains at their N-terminus. These results indicate that banana fruit cold-responsive MabHLHs may form a big protein complex in the nucleus with MaICE1. Taken together, our findings advance our understanding of the possible involvement of bHLH TFs in the regulatory network of ICE-CBF cold signaling pathway.
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