期刊
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
卷 31, 期 8, 页码 1195-1209出版社
SPRINGER
DOI: 10.1007/s11274-015-1867-5
关键词
Escherichia coli; Functional screening; Piriformospora indica; Root endophytic fungus; Salt tolerance conferring genes
资金
- University Grants Commission
- Council of Scientific and Industrial Research
- Indian Council of Medical Research, Govt. of India, New Delhi, India
- Jawaharlal Nehru University, New Delhi, India
Piriformospora indica, a root endophytic fungus identified in the Indian Thar desert, colonizes the roots of plants and provides resistance towards biotic stress as well as tolerance to abiotic stress in the plants. Despite its positive impact on the host, little is known about the P. indica genes that are involved in salt stress tolerance. Therefore this study was conducted to identify and isolate high salinity-tolerance genes from P. indica. Thirty-six salinity-tolerance genes were obtained by functional screening, based on random over expression of a P. indica cDNA library in Escherichia coli grown on medium supplemented with 0.6 M NaCl. The salinity tolerance conferred by these 36 genes in bacteria was further confirmed by using another strain of E. coli (DH5 alpha) transformants. However when the expression of these 36 genes was analysed in P. indica using quantitative RT-PCR, we found only six genes were up-regulated by salt stress. These six genes are involved in different cellular processes, such as metabolism, energy and biosynthetic processes, DNA repair, regulation of protein turnover, transport and salt stress tolerance. This work presents the basis for further molecular analyses of the mechanisms of salt tolerance in P. indica and for the use of this endophyte to confer salt tolerance to plants.
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