Article
Plant Sciences
Sanjana Negi, Steffi Madari, Himanshu Tak, Subham Bhakta, T. R. Ganapathi
Summary: The study functionally analyzed the 5'-regulatory region of the chalcone isomerase gene in banana, finding that various stress conditions and signaling molecules altered its expression. Transgenic tobacco plants harboring the gene displayed specific GUS staining in vascular region and guard cells, indicating activation of the gene under drought, salinity, and other stress conditions. Detection of multiple stress-related cis-elements in the gene region correlates with the alteration of transcript levels in banana under stress conditions.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Piotr Szymczyk, Grazyna Szymanska, Lukasz Kuzma, Agnieszka Jelen, Ewa Balcerczak
Summary: This study characterized the 5' regulatory region of the SmMEC gene and identified MeJa-responsive cis-active motifs. Co-expression studies and RT-PCR data confirmed the significant role of MeJa in SmMEC gene regulation. Furthermore, the experiment with S. miltiorrhiza solid callus cultures showed biphasic tanshinone accumulation after MeJa treatment.
Article
Plant Sciences
Yanan Jin, Xihan Ding, Jianbo Li, Zhifu Guo
Summary: This study analyzed the expression of TaIRI genes in wheat under cold stress and found that the expression was induced and accelerated by exogenous methyl jasmonate (MeJA). The promoters of TaIRI genes were isolated and their activity was confirmed through expression experiments in tobacco and Arabidopsis thaliana.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2022)
Article
Biochemistry & Molecular Biology
Sara Lago, Matteo Nadai, Emanuela Ruggiero, Martina Tassinari, Maja Marusic, Beatrice Tosoni, Ilaria Frasson, Filippo M. Cernilogar, Valentina Pirota, Filippo Doria, Janez Plavec, Gunnar Schotta, Sara N. Richter
Summary: Well-differentiated liposarcoma is a malignant neoplasm characterized by amplification of the MDM2 gene region, and targeting the MDM2 G-quadruplex can promote apoptosis in cancer cells. The research suggests that this approach may be beneficial for treating WDLPS and other tumors seeking to restore wild-type p53.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Plant Sciences
Yongil Yang, Yuanhua Shao, Timothy A. Chaffin, Jun Hyung Lee, Magen R. Poindexter, Amir H. Ahkami, Eduardo Blumwald, C. Neal Stewart Jr
Summary: Generating resistant crops to abiotic stresses is crucial for sustainable bioenergy crop production. This study developed synthetic drought stress-inducible promoters to control the precise expression of stress resistance genes in transgenic poplar, and found that shorter synthetic promoters can be used for versatile control of gene expression.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Qian Gong, Yunjing Wang, Linfang He, Fan Huang, Danfeng Zhang, Yan Wang, Xiang Wei, Meng Han, Haiteng Deng, Lan Luo, Feng Cui, Yiguo Hong, Yule Liu
Summary: This study reveals the mechanism of airborne defense (AD) against aphids and viruses. The volatile compound MeSA is perceived by neighboring plants and converted into salicylic acid, which activates a signaling cascade to induce plant anti-aphid immunity and reduce virus transmission. Aphid-transmitted viruses encode proteins that suppress AD, making plants less repellent to aphids and more suitable for aphid survival, infestation, and viral transmission.
Article
Biochemical Research Methods
Elham R. S. Soliman, Peter Meyer
Summary: The use of the RRTF1 promoter to drive the activity of the SHO gene in transgenic plants resulted in improved seed germination and survival rates under salt stress, as well as faster recovery rates, without affecting plant morphology.
PLANT MOLECULAR BIOLOGY REPORTER
(2021)
Article
Biochemistry & Molecular Biology
Wenbin Li, Pingjuan Zhao, Jianbo Sun, Xiaoling Yu, Liangping Zou, Shuxia Li, Rong Di, Mengbin Ruan, Ming Peng
Summary: The lncRNA Malnc2310 is induced by Fusarium in banana roots. Transgenic Arabidopsis with 35S::Malnc2310 accumulate more anthocyanin under stress, indicating the involvement of Malnc2310 in the flavonoid pathway. This study provides new insights into the role of Malnc2310 in mediating plant stress adaptation.
PLANT MOLECULAR BIOLOGY
(2023)
Article
Plant Sciences
Paal Krokene, Ketil Kohmann, Ngan Bao Huynh, Melissa H. Mageroy
Summary: The large pine weevil is a major pest in commercial forestry and traditional pesticide application is being phased out. This study explored alternative plant protection strategies and found that applying methyl jasmonate on 2-year-old Norway spruce plants can significantly enhance inducible defenses and inhibit sapwood growth. The effect of methyl jasmonate was stronger compared to salicylic acid and oxalic acid.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Environmental Sciences
Gagandeep Singh Dhillon, Palligarnai T. Vasudevan
Summary: The enhancement of biodiesel fuel properties through modifying/optimizing fatty ester composition is an area of ongoing research, with catalytic hydrodeoxygenation being a key method. The study conducted in this paper found that using tungsten as a catalyst supported on γ-Al(2)O(3) can effectively convert methyl oleate and biodiesel into green diesel, with factors such as reduction temperature and hydrogen flowrate influencing the activity of the catalyst.
WASTE AND BIOMASS VALORIZATION
(2021)
Article
Plant Sciences
Aysha Jameel, Toi Ketehouli, Yifan Wang, Fawei Wang, Xiaowei Li, Haiyan Li
Summary: This study utilized an ensemble approach to identify cis motifs in drought-inducible soybean genes and constructed synthetic modules to drive gene expression. The results showed that the synthetic promoters were active in responding to stress, providing tools for enhancing drought resistance in crops and advancing synthetic biology research.
FUNCTIONAL PLANT BIOLOGY
(2022)
Article
Plant Sciences
Shuang Wang, Bingbing Lv, Anhu Wang, Jianping Hu, Qi Wu, Chenglei Li
Summary: The expression of genes related to abiotic stress in plants is regulated by the transcription factor FtWRKY29, which binds to the W-box elements in their promoters. FtWRKY29 enhances the transcription of target genes and plays a crucial role in the tolerance of Tartary buckwheat to phosphorus deficiency-induced stress.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Yinzhu Cao, Guixiang Li, Xia Wang, Renwei Huang, Jianghui Luo, Mingyang Li, Daofeng Liu, Shunzhao Sui
Summary: In this study, a senescence-related gene CpSRG1 was characterized from wintersweet, showing its potential role in promoting growth and flowering while delaying senescence in transgenic Arabidopsis. The expression pattern analysis indicated that CpSRG1 was highly expressed in flower organs, especially in petals and stamens. These findings contribute to our understanding of the 2OG-Fe(II) dioxygenase superfamily and lay a foundation for further analysis of senescence-related genes in wintersweet and other wood plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Tomasz Kowalczyk, Przemyslaw Sitarek, Anna Merecz-Sadowska, Monika Szyposzynska, Aleksandra Splawska, Leslaw Gorniak, Michal Bijak, Tomasz Sliwinski
Summary: Senna obtusifolia has been used in medicine since ancient times due to its valuable compounds, such as betulinic acid, which has various health properties. This study successfully increased the accumulation of betulinic acid in hairy root cultures through transgenic cultivation with methyl jasmonate elicitation. The plant extracts obtained showed a stronger cytotoxic effect on glioblastoma cell lines and demonstrated various apoptosis-inducing activities.
Article
Genetics & Heredity
Yufei Wang, Zhiling Li, Naveed Ahmad, Xiaoxiao Sheng, Babar Iqbal, Muhammad Naeem, Nan Wang, Fengwei Li, Na Yao, Xiuming Liu
Summary: The cytochrome P450 superfamily of monooxygenases plays a crucial role in the evolution and diversification of plant natural products. This study aimed to understand the regulatory mechanism of a putative CtCYP82G24-encoding gene in safflower and its role in methyl jasmonate-induced flavonoid accumulation. The results showed that CtCYP82G24 expression increased progressively with methyl jasmonate treatment, leading to higher flavonoid and anthocyanin accumulation in transgenic plants. Virus-induced gene silencing of CtCYP82G24 resulted in reduced flavonoid accumulation and decreased expression of key flavonoid biosynthetic genes. These findings confirm the importance of CtCYP82G24 in MeJA-induced flavonoid accumulation in safflower.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2023)