Article
Plant Sciences
Aimei Tian, Hui Yu, Zhuoyue Cui
Summary: Pollen development is crucial for the sexual reproduction of seed-type plants, and protein ubiquitination is an important post-translational modification process. In this study, a gene called Bra015092 was identified and found to have higher expression in the inflorescence of Chinese cabbage. Transgenic analysis revealed that Bra015092 plays a significant role in pollen development.
PHYSIOLOGIA PLANTARUM
(2022)
Article
Environmental Sciences
Jin Chen, Pan Jin, Shimin Huang, Yeshan Guo, Fengxiao Tan, Jianwu Wang, Yinghua Shu
Summary: The study found significant differences in Cd absorption, conversion, and toxicity between two cultivars of Chinese flowering cabbage in polluted soil. The presence of the low-Cd cultivar, Lvbao701, reduced Cd accumulation and toxicity in plants and insects, potentially lowering the risks of Cd entering the food chain.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Plant Sciences
Zhidong Li, Ying Li, Tongkun Liu, Changwei Zhang, Dong Xiao, Xilin Hou
Summary: In this study, a comprehensive database called NHCCDB was developed using the high-quality genome sequence of Brassica campestris ssp. chinensis NHCC001. The database provides access to genome data, tools, and graphs, making it a valuable resource for non-heading Chinese cabbage research and breeding.
Article
Biotechnology & Applied Microbiology
Fengyan Shi, Hezi Xu, Chuanhong Liu, Chong Tan, Jie Ren, Xueling Ye, Hui Feng, Zhiyong Liu
Summary: This study aimed to identify the global landscape of mRNAs, miRNAs, lncRNAs, and circRNAs related to vernalization in Chinese cabbage. A competitive endogenous RNA network was constructed, revealing potential mRNA and non-coding RNAs involved in vernalization. Differentially expressed mRNAs, lncRNAs, circRNAs, and miRNAs related to vernalization were identified, along with important genes involved in the regulation of flowering time in Chinese cabbage.
Article
Horticulture
Chuanhong Liu, Gengxing Song, Nan Wang, Shengnan Huang, Yue Gao, Wei Fu, Meidi Zhang, Hui Feng
Summary: In this study, a wax deficiency mutant cer1 was obtained from Chinese cabbage, with genetic analysis revealing that the phenotype was controlled by a single recessive nuclear gene. The potential causal gene was identified as BraA09g066480.3C, which encodes aldehyde decarboxylase. The study shows that SNP can be effectively used for marker-assisted selection of wax deficient Chinese cabbage, facilitating the breeding of new varieties with this desirable trait.
SCIENTIA HORTICULTURAE
(2021)
Article
Microbiology
Xiaoliao Wei, Tianling Fu, Guandi He, Zhuoyan Zhong, Mingfang Yang, Fei Lou, Tengbing He
Summary: Understanding the rhizosphere soil microbial community and its relationship with the bulk soil microbial community is crucial for maintaining soil health and fertility in Karst regions. This study examined the microbial communities in the rhizosphere and bulk soils of a Chinese cabbage plantation in a Karst region and found that the rhizosphere soil had higher organic matter content and nutrient richness compared to the bulk soil. Additionally, there were differences in bacterial and fungal diversity and composition between the two types of soil.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Plant Sciences
Limin Mi, Aowei Mo, Jiange Yang, Hui Liu, Ding Ren, Wanli Chen, Haifei Long, Ning Jiang, Tian Zhang, Pingli Lu
Summary: By identifying a positive regulator, AtNMDM1, this study reveals the importance of cell wall modification in pollen intine development and male fertility regulation. The abnormal cellulose distribution in the intine was found to be associated with pollen defects, shedding light on the molecular mechanisms involved in pollen intine development.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Muhammad Ikram, Jingfang Chen, Yanshi Xia, Ronghua Li, Kadambot H. M. Siddique, Peiguo Guo
Summary: This study examined the gene expression profiles of heat-sensitive and heat-tolerant varieties of flowering Chinese cabbage under heat stress. The results revealed differential gene expression in several pathways, including MAPK signaling, plant-pathogen interactions, and plant hormone signal transduction. Additionally, several heat stress-related genes were identified, providing important insights into the heat tolerance mechanisms of flowering Chinese cabbage.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Fengyan Shi, Zifan Zhao, Yang Jiang, Song Liu, Chong Tan, Chuanhong Liu, Xueling Ye, Zhiyong Liu
Summary: This study identified mRNAs, lncRNAs, miRNAs, and circRNAs related to leaf and petiole development in Chinese cabbage and constructed a ceRNA regulatory network. Transcription factors and proteins involved in leaf and petiole development were identified, providing valuable genomic resources for further research on the molecular mechanisms underlying leaf and petiole development in this crop species.
Article
Horticulture
Shanshan Nie, Shaojie Zheng, Chunshu Lyu, Shuning Cui, Jianyu Huo, Lugang Zhang
Summary: Self-incompatibility (SI) is a genetic mechanism that promotes outcrossing and prevents self-fertilization in plants. This study investigated the relationship between calcium (Ca2+) and SI response in Chinese cabbage by altering the Ca2+ level in the stigma. The results showed that reducing the Ca2+ level in the stigma promotes pollen germination and breaks the SI response. Additionally, the expression of several BrCaM genes was found to be coincident with Ca2+ level alterations and the SI response. These findings contribute to understanding the molecular regulatory mechanism of SI response in Brassicaceae crops.
SCIENTIA HORTICULTURAE
(2023)
Article
Environmental Sciences
Min Nie, Chengxiao Hu, Guangyu Shi, Miaomiao Cai, Xu Wang, Xiaohu Zhao
Summary: Selenium can effectively scavenge excessive reactive oxygen species in root tip cells caused by chromium stress, alleviate lipid peroxidation, prevent DNA degradation, and reduce the number of apoptotic cells. Selenium restores mitochondrial dysfunction, thereby protecting plants from chromium toxicity and maintaining plant growth.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Multidisciplinary Sciences
Yun Dai, Lei Zhang, Xiao Sun, Fei Li, Shifan Zhang, Hui Zhang, Guoliang Li, Zhiyuan Fang, Rifei Sun, Xilin Hou, Shujiang Zhang
Summary: This study investigates the relationship between anthocyanin accumulation and resistance in Chinese cabbage under low-temperature conditions. The results show that low temperature upregulates the expression of genes related to anthocyanin biosynthesis, and identifies key genes involved in flavonoid and anthocyanin biosynthesis pathways. These findings are important for understanding the relationship between plant anthocyanin synthesis and stress resistance, as well as for the future development of high-quality Chinese cabbage with cold tolerance.
SCIENTIFIC REPORTS
(2022)
Article
Agronomy
Ying Zhao, Shengnan Huang, Jiaqi Zou, Shiyao Dong, Nan Wang, Hui Feng
Summary: MutMap and KASP analyses identified the BrGGL7 gene as the responsible gene for the male-sterile trait of ftms1 in Chinese cabbage, and this was further confirmed through functional verification in Arabidopsis. These findings provide important insights into the molecular mechanisms underlying male sterility in Chinese cabbage.
THEORETICAL AND APPLIED GENETICS
(2022)
Article
Biology
Jingfeng Hu, Mei Lan, Xuezhong Xu, Hongli Yang, Liqin Zhang, Fengxian Lv, Huiju Yang, Ding Yang, Chongjuan Li, Jiangming He
Summary: This study used stamen samples from male sterile and male fertile Chinese cabbage lines to investigate molecular changes during flower development, identifying weak transcriptional activity in the sterile line which may lead to abnormal stamen development. Differentially expressed genes were involved in plant hormone, carbon metabolism, and amino acid biosynthesis pathways, with key genes associated with male sterility trait. Transcription factors such as AP2, MYB, bHLH, and WRKY were found to regulate structural genes related to pollen fertility. This research provides important genomic information supporting the utilization of male sterility trait in Chinese cabbage breeding programs.
Article
Plant Sciences
Dong He, Rui Liang, Tuan Long, Ying Yang, Changyin Wu
Summary: Panicle abortion in rice is caused by defective spikelet development, with RBH1 encoding a pectate lyase protein essential for panicle formation and preventing panicle abortion.
Article
Biochemistry & Molecular Biology
Tie-Yuan Liu, Nenghui Ye, Xinyu Wang, Debatosh Das, Yuxiang Tan, Xiangkai You, Mingxiu Long, Tianming Hu, Lei Dai, Jianhua Zhang, Mo-Xian Chen
Summary: The study found that the rhizocompartments of switchgrass harbor distinct and overlapping microbial communities, with low complexity in the root compartments dominated by Proteobacteria and Firmicutes. Under drought conditions, there was selective enrichment of Cyanobacteria and Bacteroidetes in rhizosheath soil, while Proteobacteria and Firmicutes were selectively depleted. The rhizosheath microbiome assembly is driven by drought stress, with ecotype-specific recruitment revealing differences in drought stress responses.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2021)
Article
Agronomy
Haoxuan Li, Aixuan Li, Wei Shen, Nenghui Ye, Guanqun Wang, Jianhua Zhang
Summary: The study investigated alternative splicing during reproductive development in rice and identified a large number of novel splicing events, with intron retention being the most common splicing subtype. Differential expression and splicing of splicing factors could lead to global alternative splicing regulation.
Article
Plant Sciences
Tao Song, Debatosh Das, Neng-Hui Ye, Guan-Qun Wang, Fu-Yuan Zhu, Mo-Xian Chen, Feng Yang, Jian-Hua Zhang
Summary: This study conducted a comparative transcriptome analysis of coleorhiza hairs from japonica and indica rice, revealing key genes involved in water deprivation, abscisic acid, auxin metabolism, carbohydrate catabolism, and phosphorus metabolism. Up-regulation of ABA, auxin, dehydrin, and aquaporin genes may contribute to the development of coleorhiza hairs in both cultivars under air exposure, while DEGs related to glutathione and carbon metabolism are upregulated but those involved in amino acid and nucleotide sugar metabolism are downregulated, suggesting a shift towards stress-alleviating genes and less focus on primary metabolism.
Article
Plant Sciences
Zhenning Teng, Huihui Yu, Guanqun Wang, Shuan Meng, Bohan Liu, Yake Yi, Yinke Chen, Qin Zheng, Ling Liu, Jianchang Yang, Meijuan Duan, Jianhua Zhang, Nenghui Ye
Summary: The study revealed that the synergistic interaction of ABA-mediated accumulation of IAA promotes grain filling of inferior spikelets under MD. This was supported by the upregulation of genes related to starch metabolism and phytohormone signaling, as well as the downregulation of repressors of auxin signaling in the inferior spikelet. Additionally, lower trehalose content and higher levels of -6-phosphate in the inferior spikelets contributed to the improved grain filling under MD.
Article
Biochemistry & Molecular Biology
Xuanxuan Hou, Moxian Chen, Yinke Chen, Xin Hou, Zichang Jia, Xue Yang, Jianhua Zhang, Yinggao Liu, Nenghui Ye
Summary: This study identified QTLs affecting grain size in rice using F-2 intercross populations and BSA-seq technology. It also discovered splice-related mutations and new QTLs for grain width, and studied their genetic effects and pyramiding.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Yake Yi, Yaqiong Peng, Tao Song, Siqiong Lu, Zhenning Teng, Qin Zheng, Fankai Zhao, Shuan Meng, Bohang Liu, Yan Peng, Guanghui Chen, Jianhua Zhang, Nenghui Ye
Summary: This study reveals that NR-associated NO plays a crucial role in improving salt tolerance during seed germination in rice by regulating ABA catabolism. Key factors in this mechanism include nitrate, NLP2 transcription factor, and OsABA8ox1, a target gene for NLP2.
Article
Biochemistry & Molecular Biology
Zhenning Teng, Qin Zheng, Bohan Liu, Shuan Meng, Jianhua Zhang, Nenghui Ye
Summary: This study reveals the function of alternative splicing (AS) in the grain filling process of inferior spikelets and provides potential applications in increasing rice yields through genetic approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Lina Yao, Gabriel D. D'Agostino, Jinseok Park, Saiyu Hang, Arijit A. Adhikari, Yancong Zhang, Wei Li, Julian Avila-Pacheco, Sena Bae, Clary B. Clish, Eric A. Franzosa, Curtis Huttenhower, Jun R. Huh, A. Sloan Devlin
Summary: This study characterizes a biosynthetic pathway in gut bacteria that sulfonates steroidal metabolites, potentially impacting immune cell trafficking and inflammatory bowel disease.
NATURE MICROBIOLOGY
(2022)
Editorial Material
Plant Sciences
Bohan Liu, Jianhua Zhang, Nenghui Ye
TRENDS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Nenghui Ye, Yuxing Wang, Huihui Yu, Zhonge Qin, Jianhua Zhang, Meijuan Duan, Ling Liu
Summary: Salt stress is a major constraint to plant growth and production. Abscisic acid (ABA) has been shown to play a crucial role in plants under salt stress, and trehalose (Tre) has emerged as an effective osmolyte for inducing salt tolerance. However, the mechanism of interaction between ABA and Tre in rice seedlings under salt stress remains unclear. This study found that exogenous Tre significantly improved salt tolerance in rice seedlings by enhancing the activity of antioxidant enzymes. The expression of the OsNCED3 gene was induced by salt stress. Overexpression of OsNCED3 enhanced salt tolerance, while knockout of OsNCED3 reduced salt tolerance in rice seedlings. Metabolite analysis showed that Tre content was increased in OsNCED3-overexpressing seedlings and decreased in nced3 mutants. The application of both ABA and Tre improved salt tolerance in nced3 mutants compared to wild-type seedlings. The OsTPP3 gene was found to be induced by both ABA and salt treatments. Overexpression of OsTPP3 enhanced salt tolerance, while knockout of OsTPP3 reduced salt tolerance in rice seedlings. Additionally, Tre content was higher in OsTPP3-overexpressing seedlings and lower in tpp3 mutant seedlings compared to wild-type plants. Exogenous Tre application also enhanced salt tolerance in tpp3 mutant plants. Overall, these results demonstrate that salt-induced ABA activates the expression of OsTPP3, leading to an increase in Tre content and improvement in salt tolerance in rice seedlings.
Article
Agronomy
Zhenning Teng, Jiahan Lyu, Yinke Chen, Jianhua Zhang, Nenghui Ye
Summary: This review summarizes the role of abscisic acid (ABA) in root architecture development and its importance in stress response and plant growth and development. The study of ABA signaling provides insights for improving root system development and enhancing stress resistance in crop breeding.
Article
Cell Biology
Haoxuan Li, Chenjiang You, Manabu Yoshikawa, Xiaoyu Yang, Haiyong Gu, Chuanguo Li, Jie Cui, Xuemei Chen, Nenghui Ye, Jianhua Zhang, Guanqun Wang
Summary: Thermo-sensitive female sterility is a desirable trait for fully mechanized hybrid rice breeding. The study identified a spontaneous mutation, tfs1, that causes complete sterility under regular/high temperature and partial fertility under low temperature. The mutation affects the gene ARGONAUTE7 (AGO7), resulting in reduced levels of tasiR-ARFs.
Article
Agronomy
Zhenning Teng, Yinke Chen, Youqing Yuan, Yaqiong Peng, Yake Yi, Huihui Yu, Zhenxie Yi, Jianchang Yang, Yan Peng, Meijuan Duan, Jianhua Zhang, Nenghui Ye
Summary: The grain filling of inferior spikelets in rice cultivars with large panicles and numerous spikelets is less complete than that of superior spikelets, and it is promoted by moderate soil drying. This study explored the mechanism of microRNA control of grain filling of inferior spikelets in response to moderate soil drying treatment. The results showed that differentially expressed miRNAs regulated starch synthesis and phytohormone biosynthesis in inferior spikelets, which provides potential applications in increasing rice yields.
Article
Agronomy
Tao Song, A. N. M. Rubaiyath Bin Rahman, Debatosh Das, Neng-Hui Ye, Feng Yang, Mo-Xian Chen, Jian-Hua Zhang, Fu-Yuan Zhu
Summary: Coleorhiza hairs are hairlike structures in grass seeds. The molecular mechanisms of their formation are not well understood, and studying this topic can enhance our understanding of the effects of water status on rice germination during direct seeding. Transcriptomics was used to identify gene regulation during the formation of coleorhiza hairs in embryos and endosperm. Differentially expressed genes were enriched in primary and secondary metabolism, and showed changes in abscisic acid, auxin, jasmonic acid, and salicylic acid. Metabolite enrichment data were positively correlated with gene expression changes in affected metabolic pathways, indicating that root hair-associated differentially expressed genes share molecular regulators that control the formation of coleorhiza hairs.
Article
Plant Sciences
Guanqun Wang, Xiaozheng Li, Yongqiang Li, Nenghui Ye, Haoxuan Li, Jianhua Zhang
Summary: Moderate soil drying after anthesis stage improves carbon reserve remobilization in rice stems, leading to increased grain yield. Indica rice shows higher DNA methylation levels compared to japonica, with cultivar-specific methylation patterns in response to drying conditions. Specific genes such as beta-glucosidase 1 and MYBS1 play important roles in starch degradation and expression regulation under drying stress.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Biochemistry & Molecular Biology
Yanru Feng, Muhammad Shahedul Alam, Feng Yan, Michael Frei
Summary: Tropospheric ozone has significant effects on the remobilization and allocation efficiency of aboveground biomass and nutrients in cereal crops. Long-term ozone exposure increases straw C:N ratio and affects grain C:N ratio. Grain N concentrations increase significantly under ozone stress, but N yield declines due to grain yield losses. Various indicators of N use efficiency are reduced, indicating reduced N absorption from soil and allocation from vegetative to reproductive organs. Straw C:N ratio is not suitable for predicting wheat productivity. Nitrogen harvest index (NHI) is not affected by ozone stress, but the relationship between harvest index (HI) and NHI is changed by elevated ozone concentration.
Article
Biochemistry & Molecular Biology
Cong Guan, Wei Li, Guoliang Wang, Ruimei Yang, Jinglei Zhang, Jinhong Zhang, Bo Wu, Run Gao, Chunlin Jia
Summary: This study characterized the expression profiles of mRNAs and ncRNAs in switchgrass under drought stress. The up-regulated mRNAs were enriched in starch and sucrose metabolism pathway, while the differentially expressed lncRNAs potentially regulated protein-coding genes. The study also constructed regulatory networks and validated the functionality of the target gene PvSS4 in enhancing drought tolerance.
Article
Biochemistry & Molecular Biology
Juan Nicolas-Espinosa, Lucia Yepes-Molina, Fuensanta Martinez-Bernal, Miriam Fernandez-Pozurama, Micaela Carvajal
Summary: This study aimed to investigate the physiological response of broccoli leaves to abiotic stresses such as salinity and boron toxicity/deficiency. The results showed that the combined stress of salinity and boron deficiency resulted in a significant reduction in plant biomass, and the adaptation mechanisms were associated with water and boron concentration in the leaves. The expression patterns of PIP aquaporins varied among the different stress treatments, and their presence in the plasma membrane and interaction with the lipid environment played potential regulatory roles in facilitating salinity-boron stress adaptation mechanisms.
Review
Biochemistry & Molecular Biology
Wen-Feng Huang, Juan Li, Jian-An Huang, Zhong-Hua Liu, Li-Gui Xiong
Summary: This review examines the seasonal trends of phyllosphere microorganisms in woody and herbaceous plants and explores the factors influencing these trends. While herbaceous and woody plants share some similarities and differences in their phyllosphere microbiomes, further experimental validation is needed.
Article
Biochemistry & Molecular Biology
Changguang Liao, Hui Shen, Zihan Gao, Yunshu Wang, Zhiguo Zhu, Qiaoli Xie, Ting Wu, Guoping Chen, Zongli Hu
Summary: The novel CRF, SlCRF6, plays a crucial role in regulating tomato plant morphology, leaf development, and the accumulation of photosynthetic products.
Article
Biochemistry & Molecular Biology
Alok Madhu, Alok Sharma, Amandeep Kaur, Kashmir Singh, Santosh Kumar Upadhyay
Summary: In this study, 15 TaMDHAR genes were identified in bread wheat and their crucial roles in antioxidants, growth and development, and stress responses were revealed.
Article
Biochemistry & Molecular Biology
Kai Zheng, Yongsheng Cai, Yanying Qu, Lu Teng, Chaoyue Wang, Jie Gao, Quanjia Chen
Summary: In this study, the whole genome identification and bioinformatics analysis of the HCT gene family were performed in G. barbadense. The results showed that the GbHCT114 gene regulates plant trichome development, which is closely related to cotton fiber quality. Gene silencing and overexpression experiments confirmed the important role of GbHCT114 gene in cotton fiber morphology, lignin content, and secondary xylem duct cell wall development. Transcriptomic analysis identified differentially expressed genes associated with lignin synthesis and fiber development.
Review
Biochemistry & Molecular Biology
Tanashvi Seth, Sejal Asija, Shahid Umar, Ravi Gupta
Summary: Plants activate a sophisticated signaling cascade in response to pests and pathogens, with lipids playing a crucial role in mediating these defense responses. Different types of lipids are involved in cell signaling during plant-pathogen interaction and each lipid has specific relevance and contributes to specific signaling cascades. Lipid biosynthetic enzymes, including phospholipases, are involved in the production of defense signaling molecules. Lipids participate in stress signaling by mediating signal transduction, acting as precursors for bioactive molecules, regulating ROS formation, and interacting with phytohormones.
Article
Biochemistry & Molecular Biology
Yangyang Chen, Xiao Wu, Xiaohua Wang, Qionghou Li, Hao Yin, Shaoling Zhang
Summary: 'Nanguo' pears emit a rich aroma when fully ripe, and the important volatile components are the six-carbon compounds derived from the lipoxygenase pathway. This study identified a highly expressed bZIP transcription factor that is induced during the mature stage of 'Nanguo' pears, and demonstrated its regulatory role in fatty acid-derived volatile biosynthesis.
Article
Biochemistry & Molecular Biology
Zhao Geng, Haikuan Dou, Jianguang Liu, Guiyuan Zhao, Linlin Liu, Ning Zhao, Hanshuang Zhang, Yongqiang Wang, Zetong An
Summary: The overexpression of GhFB15 gene decreases the salt tolerance of Arabidopsis plants, while silencing the gene improves the salt tolerance of cotton plants. Furthermore, GhFB15 regulates the accumulation of flavonoids and the levels of ROS.
Article
Biochemistry & Molecular Biology
Linjun Cai, Ancheng Ma, Jiao Lei, Chongsheng He
Summary: METTL4 is identified as a plant DNA 6mA methyltransferase in Arabidopsis thaliana and plays a crucial role in regulating heat stress response.
Article
Biochemistry & Molecular Biology
Zailong Tian, Kun Li, Yaru Sun, Baojun Chen, Zhaoe Pan, Zhenzhen Wang, Baoyin Pang, Shoupu He, Yuchen Miao, Xiongming Du
Summary: Plants have evolved a mechanism called 'stress memory' to survive in various environmental stresses. This study reveals the physiological, biochemical, and molecular mechanisms underlying drought stress memory formation in cotton, highlighting the role of histone modification H3K4me3 in regulating transcriptional memory. It also investigates the intergenerational inheritance of drought stress memory in cotton, providing theoretical guidance for cotton breeding.