期刊
PLANT SCIENCE
卷 207, 期 -, 页码 88-97出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2013.03.002
关键词
Innate immunity; HypSys peptides; Glycopeptide signals; Plant defense; Herbivore defense
资金
- National Research Centre on Plant Biotechnology in-house research grant
- Department of Biotechnology, Ministry of Science and Technology, Government of India
- National Science Foundation [IBN 0090766, IBN 0623029]
- CharlotteY. Martin Foundation, Washington State University College of Agriculture, Human, and Natural Resources Sciences
HypSys peptides are 18-20 amino acids glycopeptide defense signal first discovered in tobacco and tomato that activate expression of defensive genes against insect-herbivores. Discovery of their orthologs in other Solanaceaous and nonsolanaceous plants demonstrated their possible ubiquitous nature and species specific functional diversity. In our continued search to establish the paradigm of defense signalling by HypSys peptides, we isolated a cDNA from potato leaves encoding putative analogs of tomato HypSys peptides flanked by conserved proteolytic cleavage sites. The gene encoding the cDNA was a member of a gene family in the tetraploid genome of potato and its expression was transcriptionally activated by wounding and methyl jasmonate. The deduced precursor protein contained a leader peptidase splice site and three putative HypSys peptides with conserved N- and C-termini along with central proline-rich motifs. In defense signalling, the three HypSys peptides elicit H2O2 generation in vivo and activate several antioxidant defensive enzymes in young potato leaves. Similar to potato systemin, the HypSys peptides activate the expression of octadecanoid pathway genes and protease inhibitors for insect defense. In addition, the HypSys peptides also activate the essential genes of the innate pathogen defense response in young potato leaves, acting as common elicitors of signalling associated with anti-herbivore and anti-pathogen defense in potato. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
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