期刊
PLANT SCIENCE
卷 210, 期 -, 页码 141-150出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2013.05.019
关键词
OsGBSS1; Oligomerization; Specific enzyme activity; Eating quality; Rice
资金
- Ministry of Science and Technology of China [2012AA10A302-2, 2012CB944803, 2011CB100202]
- National Natural Science Foundation of China [31071377]
- Ministry of Agriculture of China [2011ZX08009-003]
Granule-bound starch synthase 1 (GBSS1) is responsible for amylose synthesis in cereals, and this enzyme is regulated at the transcriptional and post-transcriptional levels. In this study, we show that GBSS1 from Ozyza sativa L. (OsGBSS1) can form oligomers in rice endosperm, and oligomerized OsGBSS1 exhibits much higher specific enzymatic activity than the monomer. A monomer-oligomer transition equilibrium for OsGBSS1 occurs in the endosperm during development. Redox potential is a key factor affecting the oligomer percentage as well as the enzymatic activity of OsGBSS1. Adenosine diphosphate glucose, the direct donor of glucose, also impacts OsGBSS1 oligomerization in a concentration-dependent manner. OsGBSS1 oligomerization is influenced by phosphorylation status, which was strongly enhanced by Mitogen-activated protein kinase (MAPK) and ATP treatment and was sharply weakened by protein phosphatase (PPase) treatment. The activity of OsGBSS1 affects the ratio of amylose to amylopectin and therefore the eating quality of rice. Understanding the regulation of OsGBSS1 activity may lead to the improvement of rice eating quality. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
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