Article
Biochemistry & Molecular Biology
Katarzyna Kabala, Malgorzata Reda, Anna Wdowikowska, Malgorzata Janicka
Summary: The up-regulation of NADPH oxidase and endogenous H2O2 seems to be an early response to salinity in cucumber plants. Increasing salt concentration leads to elevated activity of NADPH oxidase and H2O2 levels, as well as the activation of antioxidant enzymes.
Article
Plant Sciences
Balram Sahu, Subhash Chandra Naithani
Summary: This study highlights the regulatory role of ROS and its detoxifying enzymes in the process of desiccation tolerance. PEG pretreatment in germinated pea seedlings improves their desiccation tolerance by reducing the levels of superoxide and H2O2. Enhanced expression of antioxidative enzymes is associated with reduced oxidative stress. Furthermore, PEG pretreatment can reduce the accumulation of superoxide in the root.
SOUTH AFRICAN JOURNAL OF BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Matea Juric, Varun Rawat, Radhika Amaradhi, Jacek Zielonka, Thota Ganesh
Summary: In this study, a new Nox2 inhibitor, TG15-132, was discovered to prevent oxygen consumption and reactive oxygen species formation. It also showed inhibitory effects on the expression of inflammatory cytokines and iNOS. TG15-132 has a relatively long plasma half-life and excellent brain permeability, making it a potential neuroprotective agent. Furthermore, it does not exhibit toxicity on vital organs or blood biomarkers.
Review
Ecology
Colleen M. Hansel, Julia M. Diaz
Summary: Reactive oxygen species (ROS) production plays essential roles in signaling, growth, biological interactions, and physiochemical defense systems in various organisms. Additionally, biological ROS production significantly influences natural fluxes of ROS in the ocean, affecting the fate of carbon, metals, oxygen, and climate-relevant gases.
ANNUAL REVIEW OF MARINE SCIENCE, VOL 13, 2021
(2021)
Article
Medicine, Research & Experimental
Amanda Spring de Almeida, Gabriele Cheiran Pereira, Evelyne da Silva Brum, Cassia Regina Silva, Caren Tatiane de David Antoniazzi, Daniel Ardisson-Araujo, Sara Marchesan Oliveira, Gabriela Trevisan
Summary: This study investigated the anti-nociceptive effect of a repeated-dose TRPA1 antagonist administration in a model of breast cancer pain, and found that it produced an antinociceptive effect without altering TRPA1 expression in bone tissue. Using SRA strategy, no residual transcription of TRPA1 was detected in bone cell lines and mouse tissues, suggesting the potential of TRPA1 as a target for new painkillers in bone cancer treatment.
Article
Cell Biology
Yong Zhuang, Ming Wei, Chengcheng Ling, Yangxuan Liu, Abdul Karim Amin, Penghui Li, Pengwei Li, Xufan Hu, Huaxu Bao, Heqiang Huo, Jan Smalle, Songhu Wang
Summary: The gene EGY3, induced by salt and oxidative stresses, enhances salt stress tolerance in plants by promoting the stability of chloroplastic Cu/Zn-SOD2 (CSD2) and H2O2-mediated retrograde signaling, which is essential for stress tolerance.
Article
Medicine, Research & Experimental
Piotr Szczepaniak, Mateusz Siedlinski, Diana Hodorowicz-Zaniewska, Ryszard Nosalski, Tomasz P. Mikolajczyk, Aneta M. Dobosz, Anna Dikalova, Sergey Dikalov, Joanna Streb, Katarzyna Gara, Pawel Basta, Jaroslaw Krolczyk, Joanna Sulicka-Grodzicka, Ewelina Jozefczuk, Anna Dziewulska, Blessy Saju, Iwona Laksa, Wei Chen, John Dormer, Maciej Tomaszewski, Pasquale Maffia, Marta Czesnikiewicz-Guzik, Filippo Crea, Agnieszka Dobrzyn, Javid Moslehi, Tomasz Grodzicki, David G. Harrison, Tomasz J. Guzik
Summary: Cardiovascular dysfunction in breast cancer survivors treated with neoadjuvant chemotherapy is caused by the inhibitory phosphorylation of eNOS and increased ROS production by NADPH oxidases, especially by docetaxel. These effects can be prevented by Nox4 or Rock inhibitors.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Biochemistry & Molecular Biology
Anna Pato, Kata Bolcskei, Agnes Donko, Diana Kaszas, Melinda Boros, Lilla Bodrogi, Gyorgy Varady, Veronika F. S. Pape, Benoit T. Roux, Balaz Enyedi, Zsuzsanna Helyes, Fiona M. Watt, Gabor Sirokmany, Miklos Geszt
Summary: Keratinocytes of the mammalian skin produce hydrogen peroxide upon the activation of the NADPH oxidase dual oxidase 1 (DUOX1), which can be activated by increasing cytosolic calcium levels. DUOX1 knockout animals show increased sensitivity towards certain noxious stimuli, and this sensitivity is not due to structural changes in the skin. Hydrogen peroxide released by activated DUOX1 alters the activity of neuronal TRPA1 and redox-sensitive potassium channels in dorsal root ganglia primary sensory neurons, acting as a paracrine mediator of nociceptive signal transmission. These findings suggest a novel redox mechanism in modulating noxious sensory signals.
Article
Biochemistry & Molecular Biology
Gregory E. Conner
Summary: The redox status of cells is crucial for cellular activities; H2O2 metabolism regulates the redox status by modifying protein cysteine oxidation; Calcium-stimulated epithelial Duox H2O2 synthesis is transient and prevents harmful changes in redox tone following continuous stimulation.
Article
Multidisciplinary Sciences
Hidefumi Iwashita, Erika Castillo, Marco S. Messina, Raymond A. Swanson, Christopher J. Chang
Summary: A new tandem activity-based sensing and labeling strategy for H2O2 imaging has been reported, allowing capture and permanent recording of localized H2O2 fluxes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Hidefumi Iwashita, Erika Castillo, Marco S. Messina, Raymond A. Swanson, Christopher J. Chang
Summary: This study presents an advanced strategy for imaging H2O2 using a tandem activity-based sensing and labeling approach, allowing capture and recording of localized H2O2 fluxes. It enables visualization of transcellular redox signaling and provides a starting point for designing chemical probes with high spatial fidelity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Biochemistry & Molecular Biology
Peter Benko, Katalin Gemes, Attila Feher
Summary: The metabolism and regulation of cellular polyamines play a crucial role in maintaining cell homeostasis and function. Polyamine oxidases (PAOs) generate hydrogen peroxide (H2O2) during catabolic and back-conversion processes, which has been found to be involved in various plant developmental processes and stress responses. The relationship between PAOs and NADPH oxidases (RBOHs) in controlling cellular reactive oxygen species is discussed, suggesting that these enzymes regulate each other's abundance/function indirectly through H2O2.
Article
Plant Sciences
Kamilla Silva Oliveira, Renato de Mello Prado, Mirela Vantini Checchio, Priscila Lupino Gratao
Summary: The results of this study suggest that silicon can alleviate the negative effects of manganese deficiency on energy cane, improving its antioxidant capacity and manganese uptake efficiency, and promoting growth.
Article
Biochemistry & Molecular Biology
Olivia Koufos, Ryan J. Mailloux
Summary: Dihydroorotate dehydrogenase (DHODH) is involved in pyrimidine biosynthesis and can be inhibited by S-glutathionylation, which is a negative feedback loop for hydrogen peroxide (H2O2) production. This redox modification can be influenced by sex differences in liver mitochondria.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Plant Sciences
Chaofan Chen, Yi Zhang, Jianfa Cai, Yuting Qiu, Lihong Li, Chengxu Gao, Yiqun Gao, Meiyu Ke, Shengwei Wu, Chuan Wei, Jiaomei Chen, Tongda Xu, Jiri Friml, Junqi Wang, Ruixi Li, Daiyin Chao, Baocai Zhang, Xu Chen, Zhen Gao
Summary: Multi-copper oxidases SKU5 and SKS1 modulate ROS homeostasis in root apoplast to regulate cell wall formation. Loss of SKU5 and SKS1 function leads to aberrant cell wall structure and excessive ROS production, which can be rescued by decreasing ROS level or inhibiting NADPH oxidase activity.
Article
Biochemistry & Molecular Biology
Andrea A. E. Mendez, Liliana B. Pena, Lucrecia M. Curto, Marisa M. Fernandez, Emilio L. Malchiodi, Sara M. Garza-Aguilar, Jorge M. Vazquez-Ramos, Susana M. Gallego
Article
Biochemical Research Methods
Andrea A. E. Mendez, Liliana B. Pena, Lucrecia M. Curto, Marcelo D. Sciorra, Rita M. Ulloa, Sara M. Garza Aguilar, Jorge M. Vazquez Ramos, Susana M. Gallego
PROTEIN EXPRESSION AND PURIFICATION
(2020)
Article
Plant Sciences
Carolina L. Matayoshi, Liliana B. Pena, Vicent Arbona, Aurelio Gomez-Cadenas, Susana M. Gallego
Article
Plant Sciences
Liliana B. Pena, Carolina L. Matayoshi, Andrea A. E. Mendez, Martin Aran, Camila J. Moratto, Jorge M. Vazquez-Ramos, Susana M. Gallego
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Armin Fuchs, Stefan Riegler, Zahra Ayatollahi, Nicola Cavallari, Luciana E. Giono, Barbara A. Nimeth, Krishna Mutanwad, Alois Schweighofer, Doris Lucyshyn, Andrea Barta, Ezequiel Petrillo, Maria Kalyna
Summary: The study revealed the importance of RNA interference in regulating gene expression, while its interactions with alternative splicing were not well understood. By using amiRNA to knockdown various splice variants of target genes, it was found that some transcript isoforms escaped degradation due to their nuclear localization. The isoforms of alternatively spliced genes that are nuclear localized and sensitive to NMD mask the action of RNA interference.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Plant Sciences
Nabila M. Gomez Mansur, Liliana B. Pena, Adrian E. Bossio, Dalia M. Lewi, Ailin Y. Beznec, Eduardo Blumwald, Vicent Arbona, Aurelio Gomez-Cadenas, Maria P. Benavides, Susana M. Gallego
Summary: The study analyzed the metabolic changes associated with root growth restriction caused by cadmium in wheat seedlings. The IPT wheat line showed lesser reduction in root elongation compared to the WT when treated with Cd, indicating increased homeostatic range to cope with Cd stress. Hormonal imbalance at the root apex level and activation of phenolic secondary metabolism could enhance antioxidant defenses and contribute to cell wall reinforcement to deal with Cd toxicity.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Biochemistry & Molecular Biology
Federico Fuchs Wightman, Micaela A. Godoy Herz, Juan C. Munoz, Jose N. Stigliano, Laureano Bragado, Nicolas Nieto Moreno, Marcos Palavecino, Lucas Servi, Gonzalo Cabrerizo, Jose Clemente, Martin Avaro, Andrea Pontoriero, Estefania Benedetti, Elsa Baumeister, Fabian Rudolf, Federico Remes Lenicov, Cybele Garcia, Valeria Buggiano, Alberto R. Kornblihtt, Anabella Srebrow, Manuel de la Mata, Manuel J. Munoz, Ignacio E. Schor, Ezequiel Petrillo
Summary: Early detection of SARS-CoV-2 has been proven crucial during the COVID-19 pandemic, with RT-qPCR being the current gold standard. However, its demanding equipment requirements and high costs have prompted the proposal of an alternative strategy using DNA-intercalating dye. This alternative method shows similar performance to TaqMan-based detection, offering a reliable and cost-effective tool for expanding testing capabilities.
Review
Plant Sciences
Vanesa E. Tossi, Leandro Martinez Tosar, Sandra Pitta-Alvarez, Humberto F. Causin
Summary: The biosynthesis of betalains, a type of plant pigment with strong antioxidant properties, is influenced by the light environment and other factors such as phytohormones and temperature. Despite similarities between betalains and anthocyanins in function and organ distribution, the regulatory components for their production differ. Future studies on the molecular regulation of pigments may benefit from a conceptual framework summarizing the information discussed in this review.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Carolina L. Matayoshi, Liliana B. Pena, Vicent Arbona, Aurelio Gomez-Cadenas, Susana M. Gallego
Summary: Cadmium pollution poses a threat to agricultural soils, particularly affecting maize root growth during the pre-emergence stage and resulting in decreased root length, biomass, and nutrient content. Cadmium exposure also disrupts redox balance and alters hormone levels, ultimately inhibiting plant growth.
PLANT GROWTH REGULATION
(2022)
Review
Biochemistry & Molecular Biology
Sebastian Marquardt, Ezequiel Petrillo, Pablo A. Manavella
Summary: The activities of RNA polymerases have profound consequences for the epigenetic landscape of genomes. Recent studies have shown that there is coordination between transcription and RNA maturation in plants.
Article
Plant Sciences
Maria Cecilia Acosta, Vilma Teresa Manfreda, Maria Luciana Alcaraz, Sergio Alemano, Humberto Fabio Causin
Summary: Zephyranthes tubispatha seeds have optimal germination temperature range between 14 and 20 degrees C, with higher temperatures progressively inhibiting germination. Thermoinhibition is the underlying phenomenon and can be reversed by transferring seeds to 20 degrees C. Duration of high temperature incubation affects germination onset, germination rate, antioxidant enzyme activity, reactive oxygen species production, and sensitivity to germination promoters. Ethephon supply is more effective than gibberellins in suppressing thermoinhibition, suggesting the importance of changes in ethylene and abscisic acid balance/sensitivity in regulating germination responses to thermal cues in this species.
SEED SCIENCE RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Ezequiel Petrillo
Summary: This review aims to establish concepts of splicing and alternative splicing, with a focus on introns. It serves as a guide for those interested in understanding the impacts and underlying mechanisms of alternative splicing. The review critically evaluates some of the most relevant work in the field and highlights key unanswered questions, such as the physiological relevance and functional outcomes of alternative splicing, as well as the contribution of different splicing types to the proteome. The review also emphasizes the significance of intron retention as a common type of alternative splicing and proposes new sequencing technologies as a means to further investigate the role of introns in gene expression regulation.
Article
Plant Sciences
Julieta L. Mateos, Sabrina E. Sanchez, Martina Legris, David Esteve-Bruna, Jeanette C. Torchio, Ezequiel Petrillo, Daniela Goretti, Noel Blanco-Tourinan, Danelle K. Seymour, Markus Schmid, Detlef Weigel, David Alabadi, Marcelo J. Yanovsky
Summary: Plants undergo transcriptome reprograming to adapt to daily and seasonal fluctuations in light and temperature conditions. The proper spliceosome assembly, which requires the methylosome complex, is crucial for efficient pre-mRNA splicing. PICLN, a part of the methylosome complex, plays a key role in regulating pre-mRNA splicing and mediating plant adaptation to environmental conditions.
Article
Plant Sciences
Miroslav Ovecka, Jiri Sojka, Michaela Ticha, George Komis, Jasim Basheer, Cintia Marchetti, Olga Samajova, Lenka Kubenova, Jozef Samaj
Summary: The documentation of plant growth and development requires integrative and scalable approaches. Recent developments in mesoscopy, microscopy and nanoscopy methods have been applied to imaging of plant subcellular compartments, cells, tissues, and organs. However, the current LSFM and SRM modalities have limitations and there is a trend towards designing integrated microscopic modalities for broader multiscale plant imaging.
Review
Biochemistry & Molecular Biology
Rocio S. Tognacca, M. Guillermina Kubaczka, Lucas Servi, Florencia S. Rodriguez, Micaela A. Godoy Herz, Ezequiel Petrillo
TRANSCRIPTION-AUSTIN
(2020)
Article
Biochemistry & Molecular Biology
Yanru Feng, Muhammad Shahedul Alam, Feng Yan, Michael Frei
Summary: Tropospheric ozone has significant effects on the remobilization and allocation efficiency of aboveground biomass and nutrients in cereal crops. Long-term ozone exposure increases straw C:N ratio and affects grain C:N ratio. Grain N concentrations increase significantly under ozone stress, but N yield declines due to grain yield losses. Various indicators of N use efficiency are reduced, indicating reduced N absorption from soil and allocation from vegetative to reproductive organs. Straw C:N ratio is not suitable for predicting wheat productivity. Nitrogen harvest index (NHI) is not affected by ozone stress, but the relationship between harvest index (HI) and NHI is changed by elevated ozone concentration.
Article
Biochemistry & Molecular Biology
Cong Guan, Wei Li, Guoliang Wang, Ruimei Yang, Jinglei Zhang, Jinhong Zhang, Bo Wu, Run Gao, Chunlin Jia
Summary: This study characterized the expression profiles of mRNAs and ncRNAs in switchgrass under drought stress. The up-regulated mRNAs were enriched in starch and sucrose metabolism pathway, while the differentially expressed lncRNAs potentially regulated protein-coding genes. The study also constructed regulatory networks and validated the functionality of the target gene PvSS4 in enhancing drought tolerance.
Article
Biochemistry & Molecular Biology
Juan Nicolas-Espinosa, Lucia Yepes-Molina, Fuensanta Martinez-Bernal, Miriam Fernandez-Pozurama, Micaela Carvajal
Summary: This study aimed to investigate the physiological response of broccoli leaves to abiotic stresses such as salinity and boron toxicity/deficiency. The results showed that the combined stress of salinity and boron deficiency resulted in a significant reduction in plant biomass, and the adaptation mechanisms were associated with water and boron concentration in the leaves. The expression patterns of PIP aquaporins varied among the different stress treatments, and their presence in the plasma membrane and interaction with the lipid environment played potential regulatory roles in facilitating salinity-boron stress adaptation mechanisms.
Review
Biochemistry & Molecular Biology
Wen-Feng Huang, Juan Li, Jian-An Huang, Zhong-Hua Liu, Li-Gui Xiong
Summary: This review examines the seasonal trends of phyllosphere microorganisms in woody and herbaceous plants and explores the factors influencing these trends. While herbaceous and woody plants share some similarities and differences in their phyllosphere microbiomes, further experimental validation is needed.
Article
Biochemistry & Molecular Biology
Changguang Liao, Hui Shen, Zihan Gao, Yunshu Wang, Zhiguo Zhu, Qiaoli Xie, Ting Wu, Guoping Chen, Zongli Hu
Summary: The novel CRF, SlCRF6, plays a crucial role in regulating tomato plant morphology, leaf development, and the accumulation of photosynthetic products.
Article
Biochemistry & Molecular Biology
Alok Madhu, Alok Sharma, Amandeep Kaur, Kashmir Singh, Santosh Kumar Upadhyay
Summary: In this study, 15 TaMDHAR genes were identified in bread wheat and their crucial roles in antioxidants, growth and development, and stress responses were revealed.
Article
Biochemistry & Molecular Biology
Kai Zheng, Yongsheng Cai, Yanying Qu, Lu Teng, Chaoyue Wang, Jie Gao, Quanjia Chen
Summary: In this study, the whole genome identification and bioinformatics analysis of the HCT gene family were performed in G. barbadense. The results showed that the GbHCT114 gene regulates plant trichome development, which is closely related to cotton fiber quality. Gene silencing and overexpression experiments confirmed the important role of GbHCT114 gene in cotton fiber morphology, lignin content, and secondary xylem duct cell wall development. Transcriptomic analysis identified differentially expressed genes associated with lignin synthesis and fiber development.
Review
Biochemistry & Molecular Biology
Tanashvi Seth, Sejal Asija, Shahid Umar, Ravi Gupta
Summary: Plants activate a sophisticated signaling cascade in response to pests and pathogens, with lipids playing a crucial role in mediating these defense responses. Different types of lipids are involved in cell signaling during plant-pathogen interaction and each lipid has specific relevance and contributes to specific signaling cascades. Lipid biosynthetic enzymes, including phospholipases, are involved in the production of defense signaling molecules. Lipids participate in stress signaling by mediating signal transduction, acting as precursors for bioactive molecules, regulating ROS formation, and interacting with phytohormones.
Article
Biochemistry & Molecular Biology
Yangyang Chen, Xiao Wu, Xiaohua Wang, Qionghou Li, Hao Yin, Shaoling Zhang
Summary: 'Nanguo' pears emit a rich aroma when fully ripe, and the important volatile components are the six-carbon compounds derived from the lipoxygenase pathway. This study identified a highly expressed bZIP transcription factor that is induced during the mature stage of 'Nanguo' pears, and demonstrated its regulatory role in fatty acid-derived volatile biosynthesis.
Article
Biochemistry & Molecular Biology
Zhao Geng, Haikuan Dou, Jianguang Liu, Guiyuan Zhao, Linlin Liu, Ning Zhao, Hanshuang Zhang, Yongqiang Wang, Zetong An
Summary: The overexpression of GhFB15 gene decreases the salt tolerance of Arabidopsis plants, while silencing the gene improves the salt tolerance of cotton plants. Furthermore, GhFB15 regulates the accumulation of flavonoids and the levels of ROS.
Article
Biochemistry & Molecular Biology
Linjun Cai, Ancheng Ma, Jiao Lei, Chongsheng He
Summary: METTL4 is identified as a plant DNA 6mA methyltransferase in Arabidopsis thaliana and plays a crucial role in regulating heat stress response.
Article
Biochemistry & Molecular Biology
Zailong Tian, Kun Li, Yaru Sun, Baojun Chen, Zhaoe Pan, Zhenzhen Wang, Baoyin Pang, Shoupu He, Yuchen Miao, Xiongming Du
Summary: Plants have evolved a mechanism called 'stress memory' to survive in various environmental stresses. This study reveals the physiological, biochemical, and molecular mechanisms underlying drought stress memory formation in cotton, highlighting the role of histone modification H3K4me3 in regulating transcriptional memory. It also investigates the intergenerational inheritance of drought stress memory in cotton, providing theoretical guidance for cotton breeding.
