Review
Plant Sciences
Thorsten Seidel
Summary: V-ATPase is the dominant proton pump in plant cells, playing crucial roles in cytosolic pH homeostasis, endomembrane transport processes, and stress responses. Its complex structure and multiple subunit isoforms provide structural flexibility, but also require sophisticated assembly machinery and transport routes in cells.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Magdalena Zboinska, Luis C. Romero, Cecilia Gotor, Katarzyna Kabala
Summary: This study aimed to investigate the role of jasmonic acid (JA) in the regulation of V-ATPase in cucumber roots and its relationship with other regulators. The researchers found that JA acts as a repressor of V-ATPase, leading to a decrease in the protein amount of certain subunits, induction of oxidative stress, and downregulation of a specific posttranslational modification. They also suggest that H2S and H2O2 might be downstream components of JA-dependent negative regulation of the proton pump. Furthermore, they compared the signaling pathways induced by JA and cadmium, and found that multiple pathways are involved in the downregulation of V-ATPase in cucumber roots.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Katarzyna Kabala, Malgorzata Janicka
Summary: There are two ATP-dependent proton pumps in plant cells, the Plasma membrane H+-ATPase (PM H+-ATPase) and the vacuolar H+-ATPase (V-ATPase). While the PM H+-ATPase transfers protons from the cytoplasm to the apoplast, the V-ATPase pumps protons into the organelle lumen. These enzymes belong to different protein families and have distinct structures and mechanisms of action. However, they can be regulated by the same mechanisms and may coordinate in certain processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Bo Zhang, Qintao Zhang, Xinting Zhu, Dayu Li, Xiaolei Duan, Jiao Jin, Kejia Wang, Yan Xie, Yang Liu
Summary: Zinc and cadmium strongly inhibit the activity of DNA-repair protein CaPif1, a helicase responsible for genomic stability. Zinc inhibits ATPase and unwinding activities, while cadmium inhibits through binding to cysteine residues. Low concentrations of ATP can weaken zinc's inhibitory effect, while cysteine can reduce cadmium's inhibitory effect. The inhibitory effects of both metals are greater in reduced environments. This study provides insights into the mechanisms of heavy metal toxicity on DNA-repair proteins.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Environmental Sciences
Hatice Cobanoglu, Hakan Sevik, Ismail Koc
Summary: Heavy metals in air pollution pose a serious threat to the environment. This study examines the transfer of heavy metals between tree rings and their origin from traffic, as well as the variation in their concentrations in different parts of the tree and with traffic density. The results show that heavy metals can diffuse into the air due to traffic, accumulate in the wood of cedar trees, and have limited transfer within the wood. This study highlights the potential of this tree species as a monitor and mitigator of heavy metal pollution.
WATER AIR AND SOIL POLLUTION
(2023)
Article
Microbiology
Ildiko Vig, Zsigmond Benko, Barnabas Cs. Gila, Zoltan Palczert, Agnes Jakab, Fruzsina Nagy, Marton Miskei, Mi-Kyung Lee, Jae-Hyuk Yu, Istvan Pocsi, Tamas Emri
Summary: PcaA and CrpA in Aspergillus species play important roles in protecting fungal cells from heavy metal and oxidative stress, contributing to the virulence of pathogenic Aspergillus species. These P-1B-type ATPases, located on the fungal cell surface, may serve as a novel drug target in the future.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Ildiko Vig, Zsigmond Benko, Barnabas Cs. Gila, Zoltan Palczert, Agnes Jakab, Fruzsina Nagy, Marton Miskei, Mi-Kyung Lee, Jae-Hyuk Yu, Istvan Pocsi, Tamas Emri
Summary: P1B-type ATPases are important Cd2+/Cu2+ pumps in Aspergillus species, and their deletion increases sensitivity to Zn2+, Fe2+, or oxidative stress-induced by menadione sodium bisulfite and Fe3+. Heterologous expression of A. fumigatus PcaA enhances tolerance to Cd(2+) or Zn2+ stress in S. cerevisiae, while CrpA protects against oxidative stress and Fe2+ susceptibility indirectly. These P1B-type ATPases play a crucial role in protecting fungal cells from various stresses and may be potential drug targets.
MICROBIOLOGY SPECTRUM
(2023)
Article
Plant Sciences
Hui-Qian Cheng, Ying-Ning Zou, Qiang-Sheng Wu, Kamil Kuca
Summary: The research found that arbuscular mycorrhizal fungi can enhance plant resistance to drought. AMF can stimulate H+-ATPase activity and PtAHA2 gene expression, promoting nutrient uptake and root growth in plants.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Engineering, Environmental
Yang Liu, Jia Lv, Tong Cao, Yun Gong, Daliang Zhang
Summary: Ni0.22V2O5(H2O)(0.94) (NiVO) nanobelts were synthesized via hydrothermal method, and subsequent annealing led to Ni0.22V2O5 (o-NiVO) nanobelts with a large d spacing of 9.2 angstrom. The o-NiVO material exhibited advantages in electron transfer, electron conductivity, high initial capacity, good rate capability, and fast Zn2+ diffusion kinetics. It also showed excellent long-term durability.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Takuto Fujii, Kenji Sugimoto, Takafumi Noda, Takahiro Shimizu, Yuji Matsuya, Hideki Sakai
Summary: A newly synthesized dihydropyrazole derivative KYY-008 was found to selectively inhibit the activity of gastric H+,K+-ATPase, with no effect on other P2-type ATPases.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Environmental Sciences
Dandan Wang, Liugen Zheng, Mengxi Ren, Chang Li, Xianglin Dong, Xiangpin Wei, Weijun Zhou, Jiasheng Cui
Summary: In mining areas affected by long-term coal mining activities, zinc pollution poses a serious threat to soil quality and human health. This study analyzed the zinc content and isotopes composition in soil samples to understand the characteristics and sources of zinc in the soil. The results showed that zinc content exceeded the background value, and zinc primarily originated from wind dispersion and natural weathering. The use of zinc isotopes can effectively evaluate the impact of anthropogenic and natural processes on zinc in mining area soil.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Biochemistry & Molecular Biology
Guihong Liang, Haixing Song, Yan Xiao, Zhenhua Zhang
Summary: This study explored the impact of V-ATPase activity in plant vacuoles on growth and development. The lack of two tonoplast-localized subunits of V-ATPase resulted in defective growth and excessive ammonium accumulation in plants, which could be alleviated by potassium supplementation. This research provided new insights into the relationship between V-ATPase, growth, and ammonium accumulation, while highlighting the role of potassium in mitigating ammonium toxicity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Eve A. Maunders, Katherine Ganio, Andrew J. Hayes, Stephanie L. Neville, Mark R. Davies, Richard A. Strugnell, Christopher A. McDevitt, Aimee Tan
Summary: This study revealed the critical role of the P-type ATPase ZntA in maintaining bacterial metal homeostasis and resisting extracellular zinc stress in Klebsiella pneumoniae.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biology
Yong Zi Tan, Yazan M. Abbas, Jing Ze Wu, Di Wu, Kristine A. Keon, Geoffrey G. Hesketh, Stephanie A. Bueler, Anne-Claude Gingras, Carol Robinson, Sergio Grinstein, John L. Rubinstein
Summary: CryoEM analysis revealed an interaction between mEAK-7 and V-ATPase, which is disrupted by ATP-induced rotation. Exogenous mEAK-7 does not inhibit V-ATPase activity unlike its yeast homolog.
LIFE SCIENCE ALLIANCE
(2022)
Review
Plant Sciences
Satoru N. Kinoshita, Toshinori Kinoshita
Summary: Cytosolic and extracellular pH homeostasis is vital for various physiological processes in plants. The plasma membrane H+-ATPase plays a critical role in maintaining pH homeostasis and its activity is regulated by protein abundance and post-translational modifications. Phosphorylation of the penultimate threonine of the carboxy terminus can activate the plasma membrane H+-ATPase. This review summarizes recent findings on the regulation of plasma membrane H+-ATPase in photosynthetic tissues of plants, with a focus on its mechanisms and physiological roles.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Plant Sciences
Anna Wdowikowska, Malgorzata Janicka
Summary: Antisense oligonucleotide (ASO) is a short single-stranded DNA molecule that can modulate gene expression through various mechanisms; the technology based on ASO has been widely used in mammalian cell research and therapeutic strategies and is starting to be applied in plant biology, including gene function analysis and the development of non-transgenic plant production technology.
FUNCTIONAL PLANT BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Katarzyna Kabala, Malgorzata Reda, Anna Wdowikowska, Malgorzata Janicka
Summary: The up-regulation of NADPH oxidase and endogenous H2O2 seems to be an early response to salinity in cucumber plants. Increasing salt concentration leads to elevated activity of NADPH oxidase and H2O2 levels, as well as the activation of antioxidant enzymes.
Article
Biochemistry & Molecular Biology
Malgorzata Janicka, Malgorzata Reda, Natalia Napieraj, Adrianna Michalak, Dagmara Jakubowska, Katarzyna Kabala
Summary: Cucumber plants can reduce the toxic effects of Cd through removing excess toxic ions and increasing the activity of PM H+-ATPase under cadmium stress. This process is influenced by the activity of DAO, which promotes the formation of NO through H2O2 production. The stimulating effect of Cd on PM H+-ATPase activity and expression of CsHA2, CsHA4, and CsHA8 genes can be inhibited by the DAO inhibitor AG.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Katarzyna Kabala, Malgorzata Janicka
Summary: There are two ATP-dependent proton pumps in plant cells, the Plasma membrane H+-ATPase (PM H+-ATPase) and the vacuolar H+-ATPase (V-ATPase). While the PM H+-ATPase transfers protons from the cytoplasm to the apoplast, the V-ATPase pumps protons into the organelle lumen. These enzymes belong to different protein families and have distinct structures and mechanisms of action. However, they can be regulated by the same mechanisms and may coordinate in certain processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Karolina Malas, Katarzyna Kabala
Summary: In this study, two cucumber proteins, CsMIT1 and CsMIT2, which are homologous to Mitoferrin in Arabidopsis, rice and yeast, were identified and characterized. These proteins are expressed in all organs of cucumber seedlings and their expression is regulated by iron availability. The mitochondrial localization of cucumber mitoferrins was confirmed using Arabidopsis protoplasts. Furthermore, the expression of CsMIT1 and CsMIT2 can restore the growth of a mutant with defective mitochondrial iron transport, indicating the involvement of cucumber proteins in iron transport from the cytoplasm to the mitochondria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Anna Jurga, Krzysztof Ratkiewicz, Anna Wdowikowska, Malgorzata Reda, Malgorzata Janicka, Piotr Chohura, Kamil Janiak
Summary: Plant cultivation is crucial for future space missions, and an efficient food system cannot be achieved without it. The production of fertilizer in space involves extracting water and nutrients from wastewater. A study examined the effects of potential factors, such as anionic surfactants and nutrient deficiencies, on plant growth in a hydroponic system. Results showed that a deficiency of elements was the main cause of decreased yield productivity, while the surfactant had no negative effects on lettuce yield or quality.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Article
Plant Sciences
Anna Wdowikowska, Malgorzata Reda, Katarzyna Kabala, Piotr Chohura, Anna Jurga, Kamil Janiak, Malgorzata Janicka
Summary: Water and nutrient deficiencies in soil pose a serious threat to crop production, so it is important to consider the recovery of usable water and nutrients from wastewater, such as urine and grey water. This study demonstrated the possibility of using processed grey water and urine for cucumber cultivation, after dilution and supplementation with macro- and micro-elements. The resulting modified medium, enriched with nitrified urine and grey water, showed similar plant growth to that of plants grown on Hoagland solution and commercial fertilizer. However, the modified medium contained a high amount of sodium ions, leading to salt stress in cucumber plants and affecting various physiological parameters.
Review
Plant Sciences
Natalia Napieraj, Malgorzata Janicka, Malgorzata Reda
Summary: Numerous environmental conditions negatively affect plant production, causing damage at the physiological, biochemical and molecular level and limiting plant growth, development and survival. Small amine compounds, polyamines (PAs), have been found to play a key role in plant tolerance to abiotic stresses through their regulation of gene expression, ion channels, membrane stability, and interaction with signaling molecules and plant hormones. The crosstalk between PAs and plant hormones, such as abscisic acid, brassinosteroids, ethylene, jasmonates, and gibberellins, in plants under abiotic stress has been increasingly reported.
Article
Biochemistry & Molecular Biology
Magdalena Zboinska, Luis C. Romero, Cecilia Gotor, Katarzyna Kabala
Summary: This study aimed to investigate the role of jasmonic acid (JA) in the regulation of V-ATPase in cucumber roots and its relationship with other regulators. The researchers found that JA acts as a repressor of V-ATPase, leading to a decrease in the protein amount of certain subunits, induction of oxidative stress, and downregulation of a specific posttranslational modification. They also suggest that H2S and H2O2 might be downstream components of JA-dependent negative regulation of the proton pump. Furthermore, they compared the signaling pathways induced by JA and cadmium, and found that multiple pathways are involved in the downregulation of V-ATPase in cucumber roots.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Malgorzata Janicka, Malgorzata Reda, Emilia Mroczko, Anna Wdowikowska, Katarzyna Kabala
Summary: When plants are exposed to environmental stress, the presence of jasmonic acid (JA) restricts plant growth by inhibiting key proteins involved in growth regulation. In cucumber seedlings, JA treatment limits growth and increases reactive oxygen species (ROS) content in roots. JA also affects the activity and gene expression of proteins, such as plasma membrane proton pump and nitrate transporters, leading to root growth retardation.
Article
Biochemistry & Molecular Biology
Natalia Napieraj, Malgorzata Janicka, Beata Augustyniak, Malgorzata Reda
Summary: This study reveals the important regulatory role of exogenous putrescine (Put) in nitric oxide (NO) production in cucumber roots under salt stress conditions. Put reduces NO levels in short-term salt stress, but increases NO levels in long-term salt stress. Additionally, Put modulates NO biosynthesis pathways and affects nitrate reductase (NR) activity to regulate salt stress response.
Article
Plant Sciences
Magdalena Zboinska, Anna Janeczko, Katarzyna Kabala
Summary: This study aimed to clarify the role of nitric oxide (NO) in the regulation of vacuolar H+-ATPase (V-ATPase) in cucumber seedlings' roots under control and Cd stress conditions. The study also verified the relationships between NO and salicylic acid (SA), hydrogen sulfide (H2S), and hydrogen peroxide (H2O2). The results indicate that NO functions as a positive regulator of V-ATPase, and its regulation depends on NO generated by nitrate reductase and NOS-like activity. In cucumber roots, NO signaling interacts with the SA pathway and, to a lesser extent, with H2O2 and H2S.
Article
Biochemistry & Molecular Biology
Yanru Feng, Muhammad Shahedul Alam, Feng Yan, Michael Frei
Summary: Tropospheric ozone has significant effects on the remobilization and allocation efficiency of aboveground biomass and nutrients in cereal crops. Long-term ozone exposure increases straw C:N ratio and affects grain C:N ratio. Grain N concentrations increase significantly under ozone stress, but N yield declines due to grain yield losses. Various indicators of N use efficiency are reduced, indicating reduced N absorption from soil and allocation from vegetative to reproductive organs. Straw C:N ratio is not suitable for predicting wheat productivity. Nitrogen harvest index (NHI) is not affected by ozone stress, but the relationship between harvest index (HI) and NHI is changed by elevated ozone concentration.
Article
Biochemistry & Molecular Biology
Cong Guan, Wei Li, Guoliang Wang, Ruimei Yang, Jinglei Zhang, Jinhong Zhang, Bo Wu, Run Gao, Chunlin Jia
Summary: This study characterized the expression profiles of mRNAs and ncRNAs in switchgrass under drought stress. The up-regulated mRNAs were enriched in starch and sucrose metabolism pathway, while the differentially expressed lncRNAs potentially regulated protein-coding genes. The study also constructed regulatory networks and validated the functionality of the target gene PvSS4 in enhancing drought tolerance.
Article
Biochemistry & Molecular Biology
Juan Nicolas-Espinosa, Lucia Yepes-Molina, Fuensanta Martinez-Bernal, Miriam Fernandez-Pozurama, Micaela Carvajal
Summary: This study aimed to investigate the physiological response of broccoli leaves to abiotic stresses such as salinity and boron toxicity/deficiency. The results showed that the combined stress of salinity and boron deficiency resulted in a significant reduction in plant biomass, and the adaptation mechanisms were associated with water and boron concentration in the leaves. The expression patterns of PIP aquaporins varied among the different stress treatments, and their presence in the plasma membrane and interaction with the lipid environment played potential regulatory roles in facilitating salinity-boron stress adaptation mechanisms.
Review
Biochemistry & Molecular Biology
Wen-Feng Huang, Juan Li, Jian-An Huang, Zhong-Hua Liu, Li-Gui Xiong
Summary: This review examines the seasonal trends of phyllosphere microorganisms in woody and herbaceous plants and explores the factors influencing these trends. While herbaceous and woody plants share some similarities and differences in their phyllosphere microbiomes, further experimental validation is needed.
Article
Biochemistry & Molecular Biology
Changguang Liao, Hui Shen, Zihan Gao, Yunshu Wang, Zhiguo Zhu, Qiaoli Xie, Ting Wu, Guoping Chen, Zongli Hu
Summary: The novel CRF, SlCRF6, plays a crucial role in regulating tomato plant morphology, leaf development, and the accumulation of photosynthetic products.
Article
Biochemistry & Molecular Biology
Alok Madhu, Alok Sharma, Amandeep Kaur, Kashmir Singh, Santosh Kumar Upadhyay
Summary: In this study, 15 TaMDHAR genes were identified in bread wheat and their crucial roles in antioxidants, growth and development, and stress responses were revealed.
Article
Biochemistry & Molecular Biology
Kai Zheng, Yongsheng Cai, Yanying Qu, Lu Teng, Chaoyue Wang, Jie Gao, Quanjia Chen
Summary: In this study, the whole genome identification and bioinformatics analysis of the HCT gene family were performed in G. barbadense. The results showed that the GbHCT114 gene regulates plant trichome development, which is closely related to cotton fiber quality. Gene silencing and overexpression experiments confirmed the important role of GbHCT114 gene in cotton fiber morphology, lignin content, and secondary xylem duct cell wall development. Transcriptomic analysis identified differentially expressed genes associated with lignin synthesis and fiber development.
Review
Biochemistry & Molecular Biology
Tanashvi Seth, Sejal Asija, Shahid Umar, Ravi Gupta
Summary: Plants activate a sophisticated signaling cascade in response to pests and pathogens, with lipids playing a crucial role in mediating these defense responses. Different types of lipids are involved in cell signaling during plant-pathogen interaction and each lipid has specific relevance and contributes to specific signaling cascades. Lipid biosynthetic enzymes, including phospholipases, are involved in the production of defense signaling molecules. Lipids participate in stress signaling by mediating signal transduction, acting as precursors for bioactive molecules, regulating ROS formation, and interacting with phytohormones.
Article
Biochemistry & Molecular Biology
Yangyang Chen, Xiao Wu, Xiaohua Wang, Qionghou Li, Hao Yin, Shaoling Zhang
Summary: 'Nanguo' pears emit a rich aroma when fully ripe, and the important volatile components are the six-carbon compounds derived from the lipoxygenase pathway. This study identified a highly expressed bZIP transcription factor that is induced during the mature stage of 'Nanguo' pears, and demonstrated its regulatory role in fatty acid-derived volatile biosynthesis.
Article
Biochemistry & Molecular Biology
Zhao Geng, Haikuan Dou, Jianguang Liu, Guiyuan Zhao, Linlin Liu, Ning Zhao, Hanshuang Zhang, Yongqiang Wang, Zetong An
Summary: The overexpression of GhFB15 gene decreases the salt tolerance of Arabidopsis plants, while silencing the gene improves the salt tolerance of cotton plants. Furthermore, GhFB15 regulates the accumulation of flavonoids and the levels of ROS.
Article
Biochemistry & Molecular Biology
Linjun Cai, Ancheng Ma, Jiao Lei, Chongsheng He
Summary: METTL4 is identified as a plant DNA 6mA methyltransferase in Arabidopsis thaliana and plays a crucial role in regulating heat stress response.
Article
Biochemistry & Molecular Biology
Zailong Tian, Kun Li, Yaru Sun, Baojun Chen, Zhaoe Pan, Zhenzhen Wang, Baoyin Pang, Shoupu He, Yuchen Miao, Xiongming Du
Summary: Plants have evolved a mechanism called 'stress memory' to survive in various environmental stresses. This study reveals the physiological, biochemical, and molecular mechanisms underlying drought stress memory formation in cotton, highlighting the role of histone modification H3K4me3 in regulating transcriptional memory. It also investigates the intergenerational inheritance of drought stress memory in cotton, providing theoretical guidance for cotton breeding.