期刊
PLANT SCIENCE
卷 179, 期 5, 页码 527-535出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2010.08.003
关键词
Citrus sinensis; Orange fruit; Phosphoenolpyruvate carboxylase; Carbon metabolism
资金
- Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET) [PIP 06358, 02519]
- Agencia Nacional de Promocion Cientifica y Tecnologica [PICT 32459]
- CAI+D (Redes and Orientado)
Phosphoenolpyruvate (PEP) carboxylase (PEPCase) from orange fruit juice sacs has been cloned and heterogously expressed in high yield. The purified recombinant enzyme displays properties typical of plant PEPCase, including activation by sugar phosphates and inhibition by malate and citrate. Malate inhibition is weak in the physiological pH range, and the enzyme is also poorly affected by Glu and Asp, known inhibitors of C-3 plants PEPCases. However, it is strongly inhibited by citrate. Orange fruit PEPCase phosphorylation by mammalian protein kinase A decreased inhibition by malate. The enzyme presents an unusual high molecular mass in the absence of PEP, while in its presence it displays a more common tetrameric arrangement. The overall properties of the enzyme suggest that it is suited for organic acid synthesis and NADH reoxidation in the mature fruit. The present study provides the first analysis of a recombinant fruit PEPCase. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
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