期刊
PLANT SCIENCE
卷 175, 期 3, 页码 372-384出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2008.05.012
关键词
expressed sequenced tags; drought stress; horsegram; subtractive hybridization; gene expression
资金
- APNL-BTU, Hyderabad
Abiotic stresses adversely affect the agricultural productivity worldwide. Horsegram (Macrotyloma uniflorum (Lam.) Verdc.) is a legume crop that can tolerate severe adverse environmental conditions such as drought, salinity and heavy metal contamination. As a first step towards characterization of genes that contribute to combating abiotic stresses, construction and analysis of subtracted cDNA library is reported here. Using this strategy a total of 1050 ESTs were isolated, sequenced, 959 high quality ESTs were obtained and clustered. Further, our analysis revealed that of these 531 sequences are unique and 30% of these have no homology to known proteins in the database. This observation has great relevance since horsegram is a stress-adapted legume crop. Further, to validate the identified differentially expressed genes, expression profiles of selected clones were analyzed using reverse-northern, northern blot analysis and we show that indeed these clones are differentially expressed under various abiotic stress conditions. The implications of the analyzed genes in abiotic stress tolerance also discussed. (c) 2008 Elsevier Ireland Ltd. All rights reserved.
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