Structural and functional alterations induced by two sulfonamide antibiotics on barley plants

Synthetic veterinary medicines are introduced routinely in the environment after animal treatment to prevent and control infectious diseases and up to 80% the administered dose can be excreted unaltered. As a consequence, the soil is the environment most contaminated by such molecules. However, information about their implications on the growth of vegetal organisms is still scarce. With the aim of better elucidating the effects of veterinary antibiotics on plants, barley was grown in a nutrient solution containing 40 mu M (about 11,500 mu g L-1) of two well-known sulfonamide antibiotics, sulfadimethoxine (SDM) and sulfamethazine (SZ). After 15 d of treatment, the effects on root apparatus were particularly evident, while the photosynthetic tissues remained almost unaffected. SDM and SZ stimulated root hairs and lateral root development a few mm behind the root tips. In particular, from a structural point of view, treated plants showed root shortening and an advanced differentiation in comparison to controls, later confirmed using light microscopy. At a functional level, the two active molecules were found to induce root electrolyte release, such as K+, possibly due to an impairment of membrane permeability. The research concludes that sulfonamides can have profound effects on morphology and functionality of roots of crop plants. As these alterations might have consequences on their productivity, further studies are necessary to assess effects on plants at laboratory and field conditions. (C) 2013 Elsevier Masson SAS. All rights reserved.