期刊
PLANT PHYSIOLOGY AND BIOCHEMISTRY
卷 46, 期 7, 页码 724-729出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2008.04.007
关键词
Arabidopsis thaliana; aspartic protease; gene expression; nepenthesin-like protease; RT-PCR
资金
- Japan Society for Promotion of Science
There are approximately 69 genes encoding aspartyl protease homologues in Arabidopsis thaliana, and most of the gene products constitute a novel subfamily of aspartic proteases. However, their physiological roles are largely unknown. As an initial step to shed light on the roles of these nepenthesin-like aspartic proteases (NAPS), a phylogenetic tree was constructed, which indicated that these proteases are classified into several distinct sub-sub-groups. Based on these results, specific primers were designed for genes selected from several of these groups and their tissue expression was investigated using RT-PCR. The results indicated that these genes are widely expressed in several tissues, such as leaves, stems, seeds and pods, suggesting ubiquitous occurrence and multiple functions of the corresponding proteases in the tissues of A. thaliana. (c) 2008 Elsevier Masson SAS. All rights reserved.
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