Article
Biochemistry & Molecular Biology
Prakash Kumar Bhagat, Deepika Sharma, Deepanjali Verma, Kirti Singh, Alok Krishna Sinha
Summary: This study reveals the essential role of MAP kinase signaling in Arabidopsis sensitivity to higher concentrations of D-Glc. D-Glc activates MAP kinases MPK3 and MPK6 in a concentration and time-dependent manner. Mutants of mpk3 and mpk6 display low sensitivity to D-Glc, and this sensitivity is further enhanced by the addition of sucrose. The study also provides insights into the interaction between the Glc sensor protein RGS1 and MPK3/MPK6.
Article
Plant Sciences
Jing Xin, Chuanling Li, Kexin Ning, Yuan Qin, Jian-Xiu Shang, Yu Sun
Summary: Protein phosphorylation, especially serine/threonine and tyrosine phosphorylation, is crucial for signaling pathways in plant growth, development, and stress responses. DSP3, an atypical dual-specificity protein tyrosine phosphatase, negatively affects salt stress responses in Arabidopsis by modulating phosphorylated MPK3 and MPK6 levels.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Biochemistry & Molecular Biology
Jing Xin, Shanshan Guo, Xiaolei Zhang, Jiahui Tian, Yu Sun, Jian-Xiu Shang
Summary: Protein tyrosine phosphatases play crucial roles in plant growth, development, and stress responses, with DSP3 and DSP5 acting as negative regulators of plant salt tolerance. Interactions between DSP5 and MPK3/6 highlight a novel mechanism for salt stress regulation in Arabidopsis.
PLANT SIGNALING & BEHAVIOR
(2021)
Article
Plant Sciences
Julien Lang, Baptiste Genot, Jean Bigeard, Jean Colcombet
Summary: This study reveals that ETI-mediating NLRs and NLR signaling components NDR1 and EDS1 can promote the salicylic acid sector of defense downstream of MPK3 activity. It also demonstrates that both sustained and transient MPK3/6 activities positively control the expression of several NLR genes. The findings highlight the intricate interplay of PTI and ETI pathways.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Biochemistry & Molecular Biology
Xiaoyang Wang, Huicong Meng, Yuxi Tang, Yashi Zhang, Yunxia He, Jinggeng Zhou, Xiangzong Meng
Summary: Plants under pathogen attack produce high levels of ethylene to induce defense responses. The rate-limiting enzyme ACS is crucial for ethylene biosynthesis. In this study, it was found that ethylene biosynthesis induced by the fungal pathogen Botrytis cinerea in Arabidopsis is negatively regulated by the ethylene signaling pathway. The ethylene response factor ERF1A acts downstream of ethylene signaling to suppress the expression of ACS2 and ACS6. ERF1A also upregulates defensin genes and increases Arabidopsis resistance to B. cinerea. Furthermore, ERF1A is phosphorylated by MPK3/MPK6 to enhance its functions in suppressing ethylene biosynthesis and inducing defensin gene expression.
JOURNAL OF GENETICS AND GENOMICS
(2022)
Article
Plant Sciences
Yihao Li, Kun Liu, Ganlu Tong, Chao Xi, Jin Liu, Heping Zhao, Yingdian Wang, Dongtao Ren, Shengcheng Han
Summary: ERF72 functions as a positive regulator mediating resistance to the fungus Botrytis cinerea by coordinating camalexin biosynthesis and targeting the transcription factor WRKY33 in plant immunity. Phosphorylation of ERF72 by MPK3 and MPK6 at Ser151 enhances its transactivation activity, leading to increased camalexin concentration and resistance to B. cinerea.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Review
Plant Sciences
Stephen J. Bordeleau, Laura Canales E. Sanchez, Daphne R. Goring
Summary: Successful fertilization of a flowering plant requires cell-to-cell communication between pollen grain and pistil, where ligand-receptor kinase signaling plays a crucial role. While the later stages of pollen-pistil interactions have been well studied in Arabidopsis, the earlier stages remain less clear. Recent studies have implicated predicted receptor kinases in regulating pollen hydration and growth in the upper regions of the pistil. However, many questions regarding their specific functions remain unanswered.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Deepika Sharma, Dhanraj Singh, Kirti Singh, Aditi Dwivedi, Aashish Ranjan, Alok Krishna Sinha
Summary: Light is a crucial environmental factor in plant growth and development. PIFs are negative regulators involved in light responses and are essential for hypocotyl elongation in Arabidopsis. This study found that MPK6 phosphorylates and interacts with PIF3, and the phosphorylation affects its binding to miR163 promoter. Overexpression of PIF3 influences the expression of RL-responsive miRNAs and genes involved in photomorphogenesis. These results suggest that the MPK6-PIF3 module plays a role in connecting RL-responsive miRNAs and PIF3-regulated genes.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Teng-Yue Zhang, Zhong-Qi Li, Yu-Dong Zhao, Wen-Jie Shen, Meng-Shu Chen, Hai-Quan Gao, Xiao-Min Ge, Hui-Qin Wang, Xue Li, Jun-Min He
Summary: The study reveals that MPK3 and MPK6 play crucial roles in ethylene signaling by interacting with MKK1, MKK3, and EIN2 to promote stomatal closure. However, this effect is impaired in certain mutants, leading to a disruption in signal transduction pathways.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2021)
Article
Plant Sciences
Anna Ramming, Christian Kappel, Masahiro M. Kanaoka, Tetsuya Higashiyama, Michael Lenhard
Summary: Polyadenylation of mRNAs is critical for mRNA export, stability, and translation efficiency. In Arabidopsis thaliana, PAPS1 plays a key role in the acquisition of competence during pollen-tube growth and guidance. Our study shows that PAPS1 activity is required for full acquisition of competence and efficient fertilization. This research highlights the importance of functional specialization between PAPS isoforms in different developmental stages.
Article
Biochemistry & Molecular Biology
Masahiro M. Kanaoka, Kentaro K. Shimizu, Bo Xie, Sinisa Urban, Matthew Freeman, Zonglie Hong, Kiyotaka Okada
Summary: Fertilization is a key event in sexually reproducing plants. A study on Arabidopsis thaliana identified a mutant, kompeito-1 (kom-1), which showed defects in pollen wall patterning and pollen-stigma adhesion. The study found that the KOM gene is involved in the regulation of CalS5 protein accumulation, which in turn affects callose accumulation and pollen wall formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Xiu Wang, Xiangfeng Liu, Xinlei Yi, Min Wang, Wenxin Shi, Ruiping Li, Wenyue Tang, Liyao Zhang, Mengxiang Sun, Xiongbo Peng
Summary: In angiosperm, the ovules provide guidance cues for pollen tube fertilization, with the micropyle guiding the tube over short distances and the funiculus guiding it over longer distances. However, little is known about the signaling for funicular guidance. In this study, the researchers discovered that the funiculus and the female gametophyte play important roles in pollen tube guidance, with the loss of a specific gene interrupting funicular guidance. Furthermore, they found that the guiding signal for funicular guidance is generated from the female gametophyte and that all the female germ unit cells are involved in funicular guidance.
Article
Biochemistry & Molecular Biology
Kaiqi Wang, Xin Zhao, Chaoting Pang, Sida Zhou, Xuexue Qian, Nan Tang, Naiying Yang, Ping Xu, Xiaofeng Xu, Jufang Gao
Summary: The newly discovered gene IEF, regulated by AMS, plays a crucial role in exine formation and is involved in sporopollenin transportation. Its mutation leads to severe male sterility and defective pollen development, highlighting the importance of IEF in pollen development and male fertility.
PLANT MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Takuya T. Nagae, Hidenori Takeuchi, Tetsuya Higashiyama
Summary: Sexual reproduction between males and females is crucial for species maintenance, with ovular micropylar guidance playing an important role in species-preferential reproduction. A study found that fluorescein diacetate (FDA) staining is suitable for evaluating the micropylar guidance rate of non-transgenic pollen tubes. The study quantified micropylar guidance in Arabidopsis thaliana and Arabidopsis lyrata by combining FDA staining with modified semi-in vivo assays. The results showed that each species' ovules secrete species-preferential attractants, and pollen tubes respond more strongly to attractants of their own species than those of closely related species. LURE-type CRP810 attractant peptides were identified as responsible for micropylar attraction in A. thaliana.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Ming Jun Zhang, Tian Yi Zhao, Xiu Ke Ouyang, Xin-Ying Zhao, Xinren Dai, Xin-Qi Gao
Summary: The gene SKU5-SIMILAR 13 plays a critical role in pollen tube growth in the transmitting tract of Arabidopsis thaliana by mediating the biosynthesis of jasmonic acid and modifying cell wall components. Mutations in the SKS13 gene lead to altered cell wall components and retarded growth of pollen tubes, affecting male fertility during the pollen transfer process. Our results suggest that SKS13 is essential for pollen tube growth in the transmitting tract through the regulation of jasmonic acid biosynthesis.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Microbiology
Ying-Ying Cai, Jing-Yi Wang, Xi-Yu Wu, Shuang Liang, Xue-Ming Zhu, Lin Li, Jian-Ping Lu, Xiao-Hong Liu, Fu-Cheng Lin
Summary: This study reveals that MoOpy2 plays an essential role in fungal development, appressorium penetration, autophagy, and pathogenesis of Magnaporthe oryzae.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Yingmin Qu, Huijuan Cao, Pengyun Huang, Jing Wang, Xiaohong Liu, Jianping Lu, Fu-Cheng Lin
Summary: The rice blast fungus Pyricularia oryzae relies on the formation of appressorium to penetrate plant cells, and the protein PoTea1 is found to play a crucial role in this process by regulating cell polarity.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Microbiology
Pengyun Huang, Huijuan Cao, Yan Li, Siyi Zhu, Jing Wang, Qing Wang, Xiaohong Liu, Fu-Cheng Lin, Jianping Lu
Summary: This study reveals the functional diversity and complexity of melanin in different strains of Magnaporthe oryzae. Melanin promotes spore production in the rice blast pathogen, but its effect is related to the background melanin content of the wild-type strains. Transcription factors like Cnf1 have opposite effects on conidiophore differentiation in different strains. Pig1 regulates melanin synthesis in hyphae but not in conidiophores, spores, or appressoria.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Lixiao Sun, Hui Qian, Minghua Wu, Wenhui Zhao, Mengyu Liu, Yunyun Wei, Xueming Zhu, Lin Li, Jianping Lu, Fucheng Lin, Xiaohong Liu
Summary: MoIst1 plays a crucial role in sporulation, appressorium development, plant penetration, pathogenicity, and autophagy in Magnaporthe oryzae.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Qing Wang, Jing Wang, Pengyun Huang, Zhicheng Huang, Yan Li, Xiaohong Liu, Fucheng Lin, Jianping Lu
Summary: Studies have shown that Nap1 plays a crucial role in Magnaporthe oryzae, participating in regulating fungal development and virulence, especially in appressorium formation and plant penetration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Pengyun Huang, Jing Wang, Yan Li, Qing Wang, Zhicheng Huang, Hui Qian, Xiao-Hong Liu, Fu-Cheng Lin, Jianping Lu
Summary: In this study, it was found that Vrf1 and Hox7 play vital roles in the regulation of appressorium maturation in Magnaporthe oryzae. They coordinate the formation and development of appressoria by regulating the expression of genes related to cell wall remodeling.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Microbiology
Ming-Hua Wu, Qin Yu, Tian-Yi Tao, Li-Xiao Sun, Hui Qian, Xue-Ming Zhu, Lin Li, Shuang Liang, Jian-Ping Lu, Fu-Cheng Lin, Xiao-Hong Liu
Summary: This study identified and analyzed the AGC kinases in Magnaporthe oryzae, a plant-pathogenic fungus. It revealed the important roles of AGC kinases in the growth, conidiation, germination, appressorium formation, and pathogenicity of the fungus. Furthermore, it discovered the specific functions of MoFpk1, a subfamily protein of AGC kinases, in cell wall integrity and lipid metabolism. The study also provided insights into the mechanisms of plasma membrane homeostasis and autophagy through lipid asymmetry maintenance.
Article
Plant Sciences
Jing Wang, Qing Wang, Pengyun Huang, Yingmin Qu, Zhicheng Huang, Huan Wang, Xiao-Hong Liu, Fu-Cheng Lin, Jianping Lu
Summary: Defects in endosomal structure and the cAMP-PKA pathway contribute to the defective appressorium maturation and virulence of Magnaporthe oryzae.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Microbiology
Jing Wang, Zhicheng Huang, Pengyun Huang, Qing Wang, Yan Li, Xiao-Hong Liu, Fu-Cheng Lin, Jianping Lu
Summary: This study shows that the Clp1 protein in M. oryzae regulates development and pathogenicity by maintaining autophagy homeostasis and affecting gene transcription in the nucleus.
MICROBIOLOGY SPECTRUM
(2022)
Article
Plant Sciences
Hui Qian, Lixiao Sun, Minghua Wu, Wenhui Zhao, Mengyu Liu, Shuang Liang, Xueming Zhu, Lin Li, Zhenzhu Su, Jianping Lu, Fucheng Lin, Xiaohong Liu
Summary: The endoplasmic reticulum (ER) is the starting point of the secretory pathway and COPII vesicles are responsible for transporting cargo proteins from the ER to the Golgi. This study identified and explored the importance of the inner shell subunit of COPII vesicles (MoSec24B) in the rice blast fungus. The disruption of MoSec24B led to various negative effects on fungal growth, pathogenicity, and autophagy, highlighting the significant role of MoSec24B in fungal development and disease progression.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agronomy
Xin Wang, Shaodong Zhou, Jie Wang, Wenxin Lin, Xiaolei Yao, Jiaqing Su, Haiyang Li, Chao Fang, Fanjiang Kong, Yuefeng Guan
Summary: This study investigated the genetic basis of biomass accumulation in soybean plants. It identified 10 loci and 47 candidate genes related to biomass traits, and highlighted a potential candidate gene for future soybean breeding.
MOLECULAR BREEDING
(2023)
Article
Biochemistry & Molecular Biology
Zi-He Wang, Zi-Fang Shen, Jing-Yi Wang, Ying-Ying Cai, Lin Li, Jian Liao, Jian-Ping Lu, Xue-Ming Zhu, Fu-Cheng Lin, Xiao-Hong Liu
Summary: This study identified a novel calcineurin regulatory-subunit-binding protein, MoCbp7, which is involved in calcium signal transduction and is important for the growth, conidiation, appressorium formation, invasive growth, and virulence of Magnaporthe oryzae. MoCbp7 also interacts with calcineurin to regulate endoplasmic reticulum homeostasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Zhicheng Huang, Huijuan Cao, Huan Wang, Pengyun Huang, Jing Wang, Ying-Ying Cai, Qing Wang, Yan Li, Jiaoyu Wang, Xiao-Hong Liu, Fu-Cheng Lin, Jianping Lu
Summary: In this study, the researchers identified a serine/threonine protein phosphatase regulatory subunit called Smek1 that plays a crucial role in the growth, conidiation, and virulence of Magnaporthe oryzae. Deleting SMEK1 resulted in defects in the utilization of lipids, arabinose, glycerol, and ethanol. Additionally, Smek1 was found to directly interact with and dephosphorylate the proteins CreA and Crf1, which are involved in carbon metabolism. Overall, Smek1 acts as a dual-function regulator of lipid and carbohydrate metabolism, coordinating fungal development and virulence.
MOLECULAR PLANT PATHOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Zhicheng Huang, Qing Wang, Irshad Ali Khan, Yan Li, Jing Wang, Jiaoyu Wang, Xiaohong Liu, Fucheng Lin, Jianping Lu
Summary: The methylcitrate cycle and the glyoxylate cycle are important carbon metabolic pathways in fungi, which not only convert toxic intermediates into usable substances, but also play important roles in fungal growth, development, and pathogenic processes.
Letter
Biotechnology & Applied Microbiology
Zhihui Zhang, Jie Wang, Huaqin Kuang, Zhihong Hou, Pingping Gong, Mengyan Bai, Shaodong Zhou, Xiaolei Yao, Shikui Song, Long Yan, Yuefeng Guan
Summary: This study found that the soybean cultivar Huachun6 (HC6) carries the susceptible genotype of a candidate PDH1 gene, which is present in a significant portion of soybean cultivars. By utilizing genome editing techniques, several transgene-free lines with pdh1 mutations were obtained, which significantly improved the pod shattering resistance. The pdh1 mutation was found to be associated with aberrant lignin distribution in inner sclerenchyma. This research shows the potential of precision breeding through genome editing to improve pod shattering resistance and adaptability in soybean cultivars.