Review
Plant Sciences
Og-Geum Woo, Hani Kim, Jae-Hoon Lee
Summary: Ubiquitination is a crucial post-translational modification in eukaryotic cells, with the E3 ubiquitin ligase family playing a major role in substrate specificity. The CRL1 complex is the largest family in Arabidopsis and is mainly focused on the functional elucidation of F-box proteins. Research on CRL1 complexes, particularly F-box proteins, provides insights into their roles in developmental processes, hormone responses, and biotic/abiotic stress responses in plant cells.
JOURNAL OF PLANT BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Emma I. Kane, Donald E. Spratt
Summary: Ankyrin repeat (AR) domains are the most common repetitive structure in eukaryotic proteins, mediating specific protein-protein interactions and recognizing a variety of intracellular substrates. Proteins with AR domains play crucial roles in biological processes and their dysfunction is linked to neurological diseases and disorders. Understanding the structure and mechanism of key AR-containing proteins can provide insights into how they support ubiquitylation and subsequent signaling pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Melissa S. Traver, Bonnie Bartel
Summary: Lipid droplets are conserved organelles that regulate energy homeostasis by storing and releasing neutral lipids. This study identified MIEL1 as a protein that modulates lipid droplet dynamics by ubiquitinating and degrading the seed lipid droplet protein OLE1. The human homolog of MIEL1, PIRH2, was also found to be involved in lipid catabolism and peroxisome biology in mammals.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Klara Prikrylova Konecna, Agata Kilar, Petra Kovacikova, Jiri Fajkus, Eva Sykorova, Miloslava Fojtova
Summary: This study investigates the interaction between ARM and telomerase and explores the cellular functions of ARM. The results show that ARM is involved in crucial cellular processes and has a dominant effect. Additionally, ARM is implicated in circadian rhythms and responses to drugs and stimuli.
Article
Plant Sciences
Zhongyi Yang, Yingjun Chi, Yanmei Cui, Zhen Wang, Dezhou Hu, Hui Yang, Javaid Akhter Bhat, Hui Wang, Guizhen Kan, Deyue Yu, Fang Huang
Summary: The soybean E3 ubiquitin ligase, GmRNF1a, may affect pod dehiscence and seed development through MADS family genes. These findings are significant for the study of soybean pod and seed development.
Article
Biochemistry & Molecular Biology
Jan Knop, Tim Lienemann, Haifa El-Kilani, Sven Falke, Catharina Krings, Maria Sindalovskaya, Johannes Bergler, Christian Betzel, Stefan Hoth
Summary: Plant U-box armadillo repeat (PUB-ARM) ubiquitin (Ub) ligases play crucial roles in plant defense by ubiquitinating target proteins. The PUB-ARM protein SENESCENCE ASSOCIATED UBIQUITIN E3 LIGASE1 (SAUL1) can form patches at the plasma membrane related to tethering multi-vesicular bodies (MVBs) to the membrane. The structure of SAUL1 consists of two domains: a domain containing the N-terminal U-box and armadillo (ARM) repeats, and the C-terminal ARM repeat domain, which includes a positively charged groove.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Shuai Fu, Kun Wang, Tingting Ma, Yan Liang, Zhonghua Ma, Jianxiang Wu, Yi Xu, Xueping Zhou
Summary: This study identifies a microtubule-associated E3 ligase (MEL) that promotes the degradation of a serine hydroxymethyltrasferase (SHMT1), resulting in broad-spectrum resistance to pathogens. The findings suggest the potential utility of the MEL-SHMT1 module in generating broad-spectrum resistant rice to global destructive pathogens.
Article
Plant Sciences
Maria Cecilia Terrile, Nuria Malena Tebez, Silvana Lorena Colman, Julieta Lisa Mateos, Esperanza Morato-Lopez, Nuria Sanchez-Lopez, Alicia Izquierdo-Alvarez, Anabel Marina, Luz Irina A. Calderon Villalobos, Mark Estelle, Antonio Martinez-Ruiz, Diego Fernando Fiol, Claudia Anahi Casalongue, Maria Jose Iglesias
Summary: E3 ubiquitin ligases are crucial in plant biology and play a key role in the perception and signaling of hormones. The redox modification of proteins through S-nitrosation serves as an important regulatory mechanism in hormonal signaling pathways. This study confirmed the S-nitrosation of TIR1 and COI1, highlighting the functional relevance of this modification in the response to auxins and jasmonates.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agronomy
Yingchun Han, Na Liu, Chuang Li, Shuaiwu Wang, Lihua Jia, Rui Zhang, Hui Li, Jinfang Tan, Hongwei Xue, Wenming Zheng
Summary: This study identifies a MADS-box gene, TaMADS2-3D, which is rapidly induced under low-Pi stress in wheat roots. Overexpression of TaMADS2-3D in transgenic Arabidopsis and wheat leads to constitutive activation of phosphate starvation responses (PSRs), affecting root development and Pi uptake. TaMADS2-3D likely regulates PSRs by modulating ROS homeostasis, gene expression, and Pi content.
Article
Biotechnology & Applied Microbiology
Shaofei Tong, Ningning Chen, Deyan Wang, Fandi Ai, Bao Liu, Liwen Ren, Yang Chen, Junlin Zhang, Shangling Lou, Huanhuan Liu, Jianquan Liu, Tao Ma, Yuanzhong Jiang
Summary: The study revealed that PalPUB79 enhances the plant's response to drought stress by positively regulating the ABA signaling pathway in woody poplars. PalPUB79 interacts with PalWRKY77 to mediate the ABA signaling pathway and forms a negative feedback loop, providing new insights into the mechanism of ABA-mediated stress response in woody poplars.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Jumin Park, Jongbo Lee, Ji-hyung Kim, Jongbin Lee, Heeju Park, Chunghun Lim
Summary: ZNF598 plays a critical role in regulating the expression of C9ORF72-derived poly(GR) protein through ribosome-associated quality control (RQC) and promotes its degradation via the ubiquitin-proteasome pathway. Moreover, lentiviral overexpression of ZNF598 can reduce the expression of poly(GR) in C9-ALS patient-derived neurons and suppress proapoptotic caspase-3 activation.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Plant Sciences
Jae Ho Kim, Moon Seok Kim, Yong Weon Seo
Summary: Drought stress can lead to significant reductions in crop production and yield. Plant U-box proteins (PUB) play a crucial role in response to abiotic stress. However, the specific role of TaPUB4, a gene encoding the U-box and nuclear localization domains, in wheat abiotic stress response remains unknown.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Plant Sciences
Katrina J. Linden, Mon Mandy Hsia, Yi-Tze Chen, Judy Callis
Summary: The E3 ligase BRIZ in Arabidopsis thaliana plays a crucial role in plant hormone signaling pathways, particularly in suppressing ABA signaling. Mutants of briz exhibit hypersensitivity to ABA, and BRIZ acts by inhibiting ABA signaling to affect seed development and germination.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Jianghui Cui, Genzeng Ren, Yuzhe Bai, Yukun Gao, Puyuan Yang, Jinhua Chang
Summary: In this study, 59 SbPUB genes were identified in the sorghum genome. These genes were unevenly distributed on the 10 chromosomes, with most genes located on chromosome 4 and none on chromosome 5. The SbPUB genes were found to be involved in important biological processes, particularly in response to salt stress. Additionally, MYB-related elements were identified in 12 SbPUB genes, suggesting their role in regulating flavonoid biosynthesis. These findings provide a foundation for further research on salt tolerance mechanisms in sorghum.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Biochemical Research Methods
Derek VanDyke, Jonathan D. Taylor, Kyle J. Kaeo, James Hunt, Jamie B. Spangler
Summary: Targeted protein degradation (TPD) is a powerful tool for biological research and therapeutic development. It offers several advantages over functional inhibition alone, including the ability to target previously undruggable proteins and eliminate protein activity for an extended period. Biologics-based methods have expanded the TPD toolbox, allowing for depletion of extracellular and surface-exposed proteins with increased target specificity.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Plant Sciences
Subramanian Sankaranarayanan, Muhammad Jamshed, Marcus A. Samuel
Article
Biochemistry & Molecular Biology
Akane G. Mizukami, Rie Inatsugi, Jiao Jiao, Toshihisa Kotake, Keiko Kuwata, Kento Ootani, Satohiro Okuda, Subramanian Sankaranarayanan, Yoshikatsu Sato, Daisuke Maruyama, Hiroaki Iwai, Estelle Garenaux, Chihiro Sato, Ken Kitajima, Yoichi Tsumuraya, Hitoshi Mori, Junichiro Yamaguchi, Kenichiro Itami, Narie Sasaki, Tetsuya Higashiyama
Article
Plant Sciences
Jiao Jiao, Akane G. Mizukami, Subramanian Sankaranarayanan, Junichiro Yamguchi, Kenichiro Itami, Tetsuya Higashiyama
Review
Biochemistry & Molecular Biology
Subramanian Sankaranarayanan, Muhammad Jamshed, Abhinandan Kumar, Logan Skori, Sabine Scandola, Tina Wang, David Spiegel, Marcus A. Samuel
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2017)
Review
Plant Sciences
Subramanian Sankaranarayanan, Tetsuya Higashiyama
TRENDS IN PLANT SCIENCE
(2018)
Review
Plant Sciences
Subramanian Sankaranarayanan, Yan Ju, Sharon A. Kessler
FRONTIERS IN PLANT SCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Muhammad Jamshed, Subramanian Sankaranarayanan, Kumar Abhinandan, Marcus A. Samuel
Editorial Material
Biology
Subramanian Sankaranarayanan, Sharon A. Kessler
Article
Plant Sciences
Patrick Kenney, Subramanian Sankaranarayanan, Michael Balogh, Emily Indriolo
PLANT REPRODUCTION
(2020)
Article
Genetics & Heredity
Anshul Watts, Subramanian Sankaranarayanan, Archana Watts, Ritesh Kumar Raipuria
Summary: Heterologous expression involves introducing a gene from one species into the cells of another for expressing a foreign protein, with factors like codon usage and GC composition considered for optimal gene expression. Modification of codons to match the recipient species' pattern and removal of unfavorable elements are crucial for higher protein yield in heterologous systems.
Review
Plant Sciences
Kumar Abhinandan, Subramanian Sankaranarayanan, Stuart Macgregor, Daphne R. Goring, Marcus A. Samuel
Summary: This paper reviews the mechanism of self-incompatibility in plants, with a focus on the early stages of pollen-pistil interactions in Brassicaceae, revealing several key signaling events and molecular components.
TRENDS IN PLANT SCIENCE
(2022)
Editorial Material
Biochemistry & Molecular Biology
Muhammad Jamshed, Neil M. N. Hickerson, Subramanian Sankaranarayanan, Marcus A. Samuel
Summary: Exciting new research reveals the interaction between stigmatic receptors for recognizing self-incompatible pollen and the FERONIA pathway, which regulates the production of stigmatic reactive oxygen species to establish barriers against self-, intra- and interspecific pollen.
Article
Plant Sciences
Silvia Manrique, Alex Cavalleri, Andrea Guazzotti, Gonzalo H. Villarino, Sara Simonini, Aureliano Bombarely, Tetsuya Higashiyama, Ueli Grossniklaus, Chiara Mizzotti, Ana Marta Pereira, Silvia Coimbra, Subramanian Sankaranarayanan, Elisabetta Onelli, Simona Masiero, Robert G. Franks, Lucia Colombo
Summary: The gynoecium plays a critical role in the reproduction of flowering plants by containing ovules and tissues necessary for pollen germination and growth. Research has shown that HISTONE DEACETYLASE 19 (HDA19) is essential for the differentiation of ovules and the reproductive tract (ReT) by regulating the expression of SHOOT MERISTEMLESS (STM). This study provides valuable insights into plant organogenesis and tissue differentiation.
Article
Biochemistry & Molecular Biology
Subramanian Sankaranarayanan, Muhammad Jamshed, Frederic Delmas, Edward C. Yeung, Marcus A. Samuel
PLANT SIGNALING & BEHAVIOR
(2020)
Article
Biochemistry & Molecular Biology
Subramanian Sankaranarayanan, Marcus A. Samuel
PLANT SIGNALING & BEHAVIOR
(2015)