Article
Multidisciplinary Sciences
Lennart Hoengenaert, Marlies Wouters, Hoon Kim, Barbara De Meester, Kris Morreel, Steven Vandersyppe, Jacob Pollier, Sandrien Desmet, Geert Goeminne, John Ralph, Wout Boerjan, Ruben Vanholme
Summary: To improve the conversion efficiency of lignocellulosic biomass, researchers incorporated scopoletin, a compound, into the lignin polymer of Arabidopsis through genetic modification. The transgenic plants showed higher saccharification efficiency without affecting plant growth.
Article
Multidisciplinary Sciences
Siqi Zhang, Feng-Quan Tan, Ching -Hui Chung, Filip Slavkovic, Ravi Sureshbhai Devani, Christelle Troadec, Fabien Marcel, Halima Morin, Celine Camps, Maria Victoria Gomez Roldan, Moussa Benhamed, Catherine Dogimont, Adnane Boualem, Abdelhafid Bendahmane
Summary: A female-to-male transition mutant was discovered in melon, and the causal gene was identified as the carpel identity gene CRABS CLAW (CRC). The master regulator of sex determination, transcription factor WIP1, recruits the corepressor TOPLESS to suppress CRC expression, leading to male flower development. Impairing the physical interaction between TOPLESS and WIP1 results in CRC expression, carpel determination, and ultimately the development of female flowers.
Article
Multidisciplinary Sciences
Maika S. Deffieu, Ieva Cesonyte, Francois Delalande, Gaelle Boncompain, Cristina Dorobantu, Eli Song, Vincent Lucansky, Aurelie Hirschler, Sarah Cianferani, Franck Perez, Christine Carapito, Raphael Gaudin
Summary: The study combined RUSH and eRUSH methods and identified Rab7-harboring vesicles as important intermediates in the Golgi-to-plasma membrane transport of TfR. Rab7 was found to transiently associate with neosynthetic TfR-containing post-Golgi vesicles before dissociation before fusion with the plasma membrane, revealing the diversity of secretory vesicles.
Article
Plant Sciences
Aurelia Scarano, Carmela Gerardi, Eduardo Sommella, Pietro Campiglia, Marcello Chieppa, Eugenio Butelli, Angelo Santino
Summary: The metabolic and transcriptional profiling of the metabolic engineered Bronze tomato line reveals the changes in primary and secondary metabolisms during fruit ripening. The up-regulation of the shikimate synthase gene and the consumption of primary metabolites favor the production and accumulation of secondary metabolites. Additionally, the carotenoid content and ratio in the Bronze fruit also change. This study highlights the potential of the Bronze tomato line in studying the effects of these phyto-chemicals in the prevention of human pathologies.
HORTICULTURE RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Shiv Mani Dubey, Soeun Han, Nathan Stutzman, Michael J. Prigge, Eva Medvecka, Matthieu Pierre Platre, Wolfgang Busch, Matyas Fendrych, Mark Estelle
Summary: The phytohormone auxin triggers root growth inhibition within seconds via a non-transcriptional pathway. The AFB1 protein plays a crucial role in this rapid response, and its N-terminal region, including the F-box domain and residues contributing to auxin binding, is essential for its specific function. A substitution of the N-terminal region of AFB1 disrupts its cytoplasmic localization and activity in rapid root growth inhibition. Moreover, AFB1 is involved in calcium influx triggered by auxin and negatively regulates lateral root formation and transcription of auxin-induced genes.
Article
Plant Sciences
Jiehua Wang, Muhammad Moeen-ud-din, Shaohui Yang
Summary: Zinc has dose-dependent effects on both vegetative and reproductive growth of Arabidopsis, regulated by differences in auxin synthesis and transport. Exogenous IAA can alleviate zinc-induced short-root phenotype by affecting auxin accumulation, while mutants with deficient auxin mechanisms exhibit hypersensitivity to zinc.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2021)
Article
Biochemistry & Molecular Biology
Mei Hu, Yan Jiang, Jing-Jing Xu
Summary: This study demonstrated that Coq9 plays a critical role in CoQ biosynthesis in plants, and its disruption results in lower CoQ content. These findings provide new insights into the evolution of CoQ biosynthesis in plants.
Article
Plant Sciences
Ke-Lin Huang, Jing Tian, Huan Wang, Yi-Fan Fu, Yang Li, Yong Zheng, Xue-Bao Li
Summary: BnFAX6 mediates fatty acid export from plastids to regulate lipid biosynthesis and release of axillary bud dormancy by enhancing linoleic acid level in Brassica napus buds.
Article
Multidisciplinary Sciences
Hanting Yang, Hongyan Li, Qian Li
Summary: This study conducted comprehensive and systematic analyses to explore the biosynthesis, accumulation, and transport pathways of flavonoid secondary metabolites in the stems and leaves of Salvia miltiorrhiza. The findings provide valuable insights for flavonoid production and cultivar selection.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Kun-Peng Jia, Jianing Mi, Shawkat Ali, Hajime Ohyanagi, Juan C. Moreno, Abdugaffor Ablazov, Aparna Balakrishna, Lamis Berqdar, Alessia Fiore, Gianfranco Diretto, Claudio Martinez, Angel R. de Lera, Takashi Gojobori, Salim Al-Babili
Summary: This study discovered a ABA biosynthetic pathway that is independent of ABA1 and identified the intermediates of this pathway as apocarotenoids. These apocarotenoids are successively converted into ABA in both Arabidopsis and rice, exerting ABA biological functions such as maintaining seed dormancy and inducing the expression of ABA-responsive genes. Furthermore, these apocarotenoids also exhibit ABA-independent regulatory activities.
Article
Biochemistry & Molecular Biology
Shuai Wang, Shuying Han, Xiangui Zhou, Changjiang Zhao, Lina Guo, Junqi Zhang, Fei Liu, Qixin Huo, Wensheng Zhao, Zejian Guo, Xujun Chen
Summary: The rice transcription factor gene OsWRKY31 is a key component in the MPK signaling pathway involved in rice disease resistance. OsWRKY31 is phosphorylated and ubiquitinated in the defense signaling pathway mediated by OsMKK10-2.
Article
Biology
Fu Guo, Hua Wang, Guiwei Lian, Gui Cai, Wu Liu, Haidao Zhang, Dandan Li, Chun Zhou, Ning Han, Muyuan Zhu, Yinghua Su, Pil Joon Seo, Lin Xu, Hongwu Bian
Summary: Molecular and cellular characterization of callus formation in rice reveals that callus derived from the scutellum follows an embryo-like developmental program, while callus derived from the vasculature follows a root organogenesis program. The auxin signaling pathway triggers cell division in the scutellum's epidermis to form an embryo-like structure and initiate callus formation. Specific genes, such as OsLEC1 and OsIAA11, are involved in regulating callus initiation from scutellum and vasculature, respectively.
COMMUNICATIONS BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Sebastian Gross, Fabian Panter, Domen Pogorevc, Carsten E. Seyfert, Selina Deckarm, Chantal D. Bader, Jennifer Herrmann, Rolf Mueller
Summary: The development of new antibiotics is crucial in combating infections caused by multidrug-resistant bacteria, with Gram-negative pathogens posing particular challenges. Through synthetic engineering of darobactin biosynthetic gene cluster in Escherichia coli, high yields of darobactin A and its active derivatives have been successfully produced, showing improved efficacy against various bacteria. This study also sheds light on the potential self-resistance mechanism in native darobactin producers.
Article
Plant Sciences
Linkan Dash, Sivakumar Swaminathan, Jan Simura, Caitlin Leigh P. Gonzales, Christian Montes, Neel Solanki, Ludvin Mejia, Karin Ljung, Olga A. Zabotina, Dior R. Kelley
Summary: The GATU10 gene plays an important role in Arabidopsis root development and its mutation leads to restricted root growth. The study found that reduced root growth in the mutant was due to a decrease in both root apical meristem size and epidermal cell elongation. Furthermore, GATU10 affected the composition of pectin and hemicellulose in the root. Molecular analysis also revealed associations between the GATU10 mutant and genes related to auxin metabolism and peroxisome function. Overall, these findings suggest that pectin composition may influence auxin pathways and peroxisome activity.
Article
Multidisciplinary Sciences
Hengwei Zou, Hanxiao Jiang, Liangbo Li, Rongshao Huang
Summary: The study found that the Aspergillus flavus elicitor 1-G4 can promote the growth and development of Bletilla striata, increasing the contents of SA, ICAld, and ME-IAA while decreasing IP and ACC contents. By analyzing transcriptomics and metabolomics, key genes involved in B. striata growth were identified, laying a foundation for the effective utilization of endophytic fungi and the optimization of B. striata germplasm resources.
SCIENTIFIC REPORTS
(2021)
Article
Biochemical Research Methods
Daniel Abate-Pella, Dana M. Freund, Janet P. Slovin, Adrian D. Hegeman, Jerry D. Cohen
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2017)
Article
Biochemistry & Molecular Biology
Michael B. Wilson, Alison D. Pawlus, Doug Brinkman, Gary Gardner, Adrian D. Hegeman, Marla Spivak, Jerry D. Cohen
Article
Plant Sciences
Dana M. Freund, Amanda C. Martin, Jerry D. Cohen, Adrian D. Hegeman
Article
Plant Sciences
Luz G. Munoz-Sanhueza, YeonKyeong Lee, Molly Tillmann, Jerry D. Cohen, Anne Kathrine Hvoslef-Eide
Article
Plant Sciences
Jorge Gonzalez-Villagra, Jerry D. Cohen, Marjorie M. Reyes-Diaz
PHYSIOLOGIA PLANTARUM
(2019)
Article
Plant Sciences
Jorge Gonzalez-Villagra, Acacio Rodrigues-Salvador, Adriano Nunes-Nesi, Jerry D. Cohen, Marjorie M. Reyes-Diaz
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2018)
Article
Multidisciplinary Sciences
Dana M. Freund, Katherine A. Sammons, Nokwanda P. Makunga, Jerry D. Cohen, Adrian D. Hegeman
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2018)
Article
Chemistry, Multidisciplinary
Erin M. Evans, Dana M. Freund, Veronica M. Sondervan, Jerry D. Cohen, Adrian D. Hegeman
FRONTIERS IN CHEMISTRY
(2018)
Article
Plant Sciences
Qian Tang, Peng Yu, Molly Tillmann, Jerry D. Cohen, Janet P. Slovin
Article
Biochemical Research Methods
Molly Tillmann, Qian Tang, Jerry D. Cohen
Summary: The plant hormone auxin is crucial for plant growth and environmental response. Various pathways exist for the biosynthesis of IAA, the primary auxin, in different plant species. Techniques involving stable isotopes and chemical inhibitors are useful for tracing pathway utilization in vivo.
Article
Multidisciplinary Sciences
Minmin Du, Firas Bou Daher, Yuanyuan Liu, Andrew Steward, Molly Tillmann, Xiaoyue Zhang, Jeh Haur Wong, Hong Ren, Jerry D. Cohen, Chuanyou Li, William M. Gray
Summary: This study reveals the mechanism by which auxin regulates hypocotyl elongation and apical hook formation in etiolated seedlings. Shortly after germination, high levels of auxin inhibit elongation, providing a time window for apical hook development. Gravity-induced auxin maximum on the concave side of the hook induces gene expression that leads to differential cell elongation. Subsequently, auxin concentrations decline and switch from inhibiting to promoting elongation, thereby driving hypocotyl elongation.
Article
Plant Sciences
Jorge Gonzalez-Villagra, Rebeca Patricia Omena-Garcia, Acacio Rodrigues-Salvador, Adriano Nunes-Nesi, Jerry D. Cohen, Marjorie M. Reyes-Diaz
Summary: Drought stress affects the young and fully expanded leaves of Aristotelia chilensis plants differently, with young leaves showing more drastic reduction in net CO2 assimilation, stomatal conductance, and transpiration. Young leaves accumulate higher levels of total protein, amino acids, sucrose, and fructose, while fully expanded leaves stimulate the expression of anthocyanins and related genes.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Biochemistry & Molecular Biology
Molly Tillmann, Qian Tang, Gary Gardner, Jerry D. Cohen
Summary: This study investigated the contributions of different biosynthetic pathways to auxin synthesis in Arabidopsis plants. It found that auxin synthesis dependent on tryptophan through the IPyA pathway plays a major role in de-etiolating hypocotyls and can be effectively blocked using chemical inhibitors. The labeling treatment results showed that auxin synthesized from indole through [C-13(6)]anthranilate was more abundant than from [N-15(1)]indole.
Article
Horticulture
Renata P. Pincelli-Souza, Laise Sousa Moreira, Jerry D. Cohen
Summary: This study provides an efficient sterilization protocol for hazelnut micropropagation and addresses the issue of poor root organogenesis. By pre-treating and growing the cuttings in absolute darkness, visible roots can be successfully cultivated.
Article
Chemistry, Multidisciplinary
Sumaira Mazhar, Jerry D. Cohen, Shahida Hasnain
JOURNAL OF THE CHEMICAL SOCIETY OF PAKISTAN
(2019)