Article
Plant Sciences
Hanqi Wang, Yuejing Zhang, Zhenzhen Shi, Hailong Pang, Lingyun Jia, Hanqing Feng
Summary: Extracellular ATP acts as a repressor for seed germination in Arabidopsis by binding to its receptor, DORN1, and its regulation is influenced by external temperature. The transient increase of extracellular ATP level during germination affects hormone content and sensitivity of the seeds.
Article
Plant Sciences
Yansheng Wu, Hongmin Yin, Xinyue Liu, Jiawei Xu, Baozhi Qin, Kaili Feng, Erfang Kang, Zhonglin Shang
Summary: Extracellular ATP (eATP) functions as a signal in plant growth and development, promoting pollen germination (PG) and pollen tube growth (PTG) by stimulating Ca2+ or K+ absorption. The mechanisms underlying the eATP-stimulated ion uptake and their role in PG and PTG were investigated using Arabidopsis thaliana as a model. The results suggest that signaling components involved in eATP-promoted PG and PTG may regulate Ca2+ or K+ influx in Arabidopsis pollen grains.
Article
Plant Sciences
Takayuki Fujita, Marcel Pascal Beier, Mayumi Tabuchi-Kobayashi, Yoshitaka Hayatsu, Haruka Nakamura, Toshiko Umetsu-Ohashi, Kazuhiro Sasaki, Keiki Ishiyama, Emiko Murozuka, Mikiko Kojima, Hitoshi Sakakibara, Yuki Sawa, Akio Miyao, Toshihiko Hayakawa, Tomoyuki Yamaya, Soichi Kojima
Summary: This study investigates the unique physiological roles of GS1;3 in the early stage of seed germination and grain filling under N deficient conditions in rice, and its impact on rice growth and yield.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Kazuya Hasegawa, Ai Ichikawa, Haruki Takeuchi, Atsuko Nakamura, Hiroaki Iwai
Summary: Pectin modification and degradation are crucial for plant development, but the mechanisms behind it are not well understood. This study explored the function of pectin in early pollen development. By overexpressing the OsPME1 gene, which encodes pectin methylesterase, rice lines with reduced pectin methyl esterification were generated. These lines showed abnormal phenotypes in anther and pollen development, especially in the pollen mother-cell stage.
Article
Plant Sciences
Ming Jun Zhang, Jing Jing Cui, Zi Ming Wang, Yu Xiu Dong, Xin-Qi Gao
Summary: Arabidopsis GR1 and NTRA are involved in regulating the interaction between the pollen tube and the stigma during pollination. They play a role in the pollen tube penetrating the stigma into the transmitting tract. Although both GR1 and NTRA are expressed in pollen, further investigation is needed to understand their specific roles in pollen germination and the growth of the pollen tube. The double mutation of gr1 and ntra compromised the transmission of male gametophytes, indicating the importance of GR1 and NTRA in the pollination process.
Article
Biochemistry & Molecular Biology
Emilia Pers-Kamczyc, Jacek Kamczyc
Summary: Nitrogen plays an important role in plant reproduction. Recent studies have shown that long-term fertilizer supplementation can affect the germination ability and nitrogen content of pollen grains produced by male plants of T. baccata and J. communis. GC-MS analysis was conducted to study the metabolomes of dry, mature pollen grains in both species. The results revealed that fertilizer supplementation can impact the relative content of several metabolites in both species. The presence of different metabolites in the pollen samples indicates a significant environmental impact on the quality of gymnosperm pollen grains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Xiaoyun Tan, Haoyue Xu, Jie Ye, Jin Wang, Wenlong Liu, Feng Liu, Qingchen Rui, Yiqun Bao
Summary: The exocyst subunit SEC6 plays an important role in cell plate formation during pollen mitosis I and might help to tether the vesicles before fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Plant Sciences
A. Carolina Puentes-Romero, Sebastian A. Gonzalez, Enrique Gonzalez-Villanueva, Carlos R. Figueroa, Simon Ruiz-Lara
Summary: The research indicates that Arabidopsis ZINC FINGER 4 (AtZAT4) plays a crucial role in pollen development and maturation, with its subcellular localization in the nucleus, contributing to the regulation of genes associated with reproductive development. The study of Atzat4 (+/) heterozygous mutant revealed significant reductions in gene expression, leading to impaired pollen tube development and reduced seed viability. These findings suggest a role for AtZAT4 in fertilization and seed viability through the regulation of gene expression during reproductive development.
Article
Plant Sciences
Yingjing Miao, Jiashu Cao, Li Huang, Youjian Yu, Sue Lin
Summary: In this study, a new Arabidopsis FLA gene, FLA14, was identified as a pollen grain-specific gene with localization along the cell membrane and in Hechtian strands. Overexpression of FLA14 resulted in abnormal pollen grains with reduced fertility, while loss-of-function mutant showed precocious pollen germination under high moisture conditions inside the mature anthers. These findings suggest a role for FLA14 in pollen development and preventing premature pollen germination.
Article
Cell Biology
Huaqiang Ruan, Ting Wang, Haiyun Ren, Yi Zhang
Summary: Pollen germination requires cytosolic calcium concentration and vesicle dynamics, which are correlated and play important roles in pollen cell growth and fertilization.
Article
Plant Sciences
Xiulin Tian, Xingjuan Wang, Yan Li
Summary: The study revealed that myosin XI drives the movement of vesicles and peroxisomes in Arabidopsis pollen tubes, suggesting that this mechanism may be evolutionarily conserved in plant cells.
Article
Plant Sciences
Zongyun Yan, Meng Jing, Bangyue Zhang, Huiying Shi, Xu Jin, Xiaoyuan Yan, Tiao Gao, Yuzhen Han
Summary: Members of the LARP1 family have been found to play a role in the regulation of seed germination in Arabidopsis, with higher abundance in seeds. They may also be involved in RNA processes.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Plant Sciences
Oyeyemi O. Ajayi, Michael A. Held, Allan M. Showalter
Summary: The study investigated the biochemical and physiological roles of three Golgi-localized beta-glucuronosyltransferase genes in Arabidopsis, showing alterations in the structure and content of AGPs in mutant plants. These mutations resulted in tissue-specific changes, especially in arabinose and galactose sugars, highlighting the importance of these genes in plant growth and development.
Article
Agronomy
Xue Luo, Ya-Nan Bai, Kai Sun, Wei Zhang, Chuan-Chao Dai
Summary: Long-term monocropping reduces flower formation, pollen viability, pollen germination, and tube growth, which decreases seed set in peanut. Genes associated with anther dehiscence, heat stress response, flavonoid biosynthesis, glutathione metabolism, and potassium uptake are enriched in flowers under monocropping, indicating water deficit and oxidative stress. Inhibition of reactive oxygen species synthesis or scavenging excess reactive oxygen species rescues pollen activity decline. Soil structure alterations, especially the reduction of large macro-aggregates (>2 mm), mediate flower water deficit and oxidative stress under long-term monocropping.
Article
Biochemistry & Molecular Biology
Pei-Yuan Chen, Chun-Yi Hsu, Cheng-En Lee, Ing-Feng Chang
Summary: The Arabidopsis Glade Ill glutamate receptor GLR3.7 is involved in salt stress response and ABA response, with Ser-860 potentially playing an important role in ABA response.
PLANT SIGNALING & BEHAVIOR
(2021)
Article
Biochemistry & Molecular Biology
Josip Safran, Olivier Habrylo, Mehdi Cherkaoui, Sylvain Lecomte, Aline Voxeur, Serge Pilard, Solene Bassard, Corinne Pau-Roblot, Davide Mercadante, Jerome Pelloux, Fabien Senechal
Summary: The study identified two novel enzymes, VdPG2 and VdPME1, from the fungal plant pathogen Verticillium dahliae, which play different roles in degrading pectin during pathogen attack on plants. These enzymes may induce plant defense responses by releasing different types of oligogalacturonides (OGs).
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Applied
Catalina Duran Garzon, Olivier Habrylo, Adrien Lemaire, Anais Guillaume, Yoann Carre, Clemence Millet, Catherine Fourtot-Brun, Pauline Trezel, Pascal Le Blond, Aurore Perrin, Stephane George, Magali Wagner, Yves Coutel, Loic Levavasseur, Corinne Pau-Roblot, Jerome Pelloux
Summary: This study comprehensively characterized the enzymatic potential of Aspergillus aculeatinus strain O822 to produce RGI-degrading enzymes, revealing high levels of enzymatic activities that effectively reduced the viscosity of RGI-rich substances. Apple juice trials demonstrated increased filtration flow rate and yield, paving the way for industrial use of these enzymes derived from A. aculeatinus.
CARBOHYDRATE POLYMERS
(2021)
Article
Biology
Charlotte Toustou, Marie-Laure Walet-Balieu, Marie-Christine Kiefer-Meyer, Marine Houdou, Patrice Lerouge, Francois Foulquier, Muriel Bardor
Summary: N-glycosylation is an important post-translational modification that is highly conserved in eukaryotes, bacteria, and archaea. The processing of N-glycans in eukaryotes is sequential and involves specific steps in the secretory pathway. Recent research has also highlighted the regulation of N-glycosylation, including the biosynthesis of complex-type N-glycans and the role of TMEM165 in different eukaryotic clades.
BIOLOGICAL REVIEWS
(2022)
Article
Microscopy
Romain Roulard, Michel Trentin, Valerie Lefebvre, Francoise Fournet, Ludivine Hocq, Jerome Pelloux, Eric Husson, Christophe Pineau, Loic Dupont, Arash Jamali
Summary: This paper introduces an innovative method for in situ observation of plant cell and tissue surfaces using environmental scanning electron microscopy (ESEM), and describes the development of a suitable accessory for the experiments. Sample-specific configurations and optimized tuning of the ESEM were defined to maintain viability of Arabidopsis and flax seedlings throughout repetitive exposure to imaging conditions. The results demonstrate the potential of ESEM for in situ studies in plant science and provide guidance for researchers to optimize their electron microscopy observation in relevant fields.
Article
Plant Sciences
Marc Ropitaux, Quentin Hays, Aurelie Baron, Laura Fourmois, Isabelle Boulogne, Boris Vauzeilles, Patrice Lerouge, Jean-Claude Mollet, Arnaud Lehner
Summary: Protein tracking in living plant cells is routine, but tracking glycans is challenging due to their complex formation process. Currently, imaging of cell wall polymers relies on antibodies or dyes, which do not allow visualization of live cells. Click chemistry in plant cell wall biology offers an alternative by incorporating a chemical reporter into the target polysaccharide, allowing covalent linkage to a fluorescent probe. This method enables the imaging of cell wall polysaccharides in living and elongating cells.
Article
Chemistry, Multidisciplinary
Pengfei Tian, Adrien Lemaire, Fabien Senechal, Olivier Habrylo, Viviane Antonietti, Pascal Sonnet, Valerie Lefebvre, Frederikke Isa Marin, Robert B. Best, Jerome Pelloux, Davide Mercadante
Summary: Efficiently designing functional proteins with higher thermal stability is still challenging, especially for highly diverse sequence variants. However, optimizing sequence design based on evolutionary fitness can help improve protein stability. In this study, we used a generative evolution fitness model to design artificial sequences for a proteinaceous inhibitor of pectin methylesterase enzymes. The majority of the designs were functional, and some even showed improved thermal stability. This method has the potential to expand the sequence space of functional proteins with valuable traits for industrial applications and scientific research.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemistry & Molecular Biology
Jeanne Tonnabel, Pascal Cosette, Arnaud Lehner, Jean-Claude Mollet, Mohamed Amine Ben Mlouka, Lucija Grladinovic, Patrice David, John R. Pannell
Summary: Sexual selection plays a significant role in driving phenotypic variation in nature, not only in animals but also in plants. Our study reveals that the post-pollination phase of plant mating is an important arena for sexual selection.
Article
Plant Sciences
Francois Jobert, Alexandre Soriano, Laurent Brottier, Celia Casset, Fanchon Divol, Josip Safran, Valerie Lefebvre, Jerome Pelloux, Stephanie Robert, Benjamin Peret
Summary: The emergence of secondary roots is a regulated process that involves cell wall modifications and hormonal control. In white lupin, the emergence of closely spaced rootlets is associated with the upregulation of genes involved in cell wall pectin modification and degradation, under the active control of auxin. The demethylesterification of pectin and modifications in cortical cells also contribute to the outgrowth of rootlet primordia.
Article
Plant Sciences
A. Paterlini, J. Sechet, F. Immel, M. S. Grison, S. Pilard, J. Pelloux, G. Mouille, E. M. Bayer, A. Voxeur
Summary: This study provides a biochemical characterization of the cell wall associated with PD in Arabidopsis thaliana cell cultures. The results show specific molecular composition patterns in the PD fraction, with fucosylated xyloglucans, short methylated stretches in homogalacturonans, and abundant rhamnogalacturonan I species. The study also found that these PD-associated wall polysaccharides are less affected by wall modifying activities compared to the broad wall.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Mathieu Carlier, Thomas Poisson, Jean-Claude Mollet, Patrice Lerouge, Cyrille Sabot, Arnaud Lehner
Summary: Glycan metabolic engineering is a powerful tool for studying glycosylation in plant cells. Modified monosaccharides have been used to study carbohydrate metabolism. In this study, 2F-Fuc was found to inhibit plant cell elongation by inhibiting Golgi-localized fucosyltransferases. A caged 1-OH-2F-Fuc derivative, 2F-Fuc-NB, was synthesized for spatio-temporal inhibition of cell elongation. The photorelease of 2F-Fuc-NB effectively inhibited root cell elongation in a dose-dependent manner.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Lothar Kalmbach, Matthieu Bourdon, Ilya Belevich, Jerome Pelloux, Eija Jokitalo, Josip Safran, Adrien Lemaire, Jung-ok Heo, Sofia Otero, Bernhard Blob, Yka Helariutta
Summary: In plants, the phloem relies on specialized cells called sieve elements for long-distance distribution of photosynthetic products. This study identifies PLL12 as a critical gene for sieve element differentiation and suggests its vital role in pectin remodeling. While short distance symplastic diffusion is unaffected, PLL12 appears to act as a regulator for local pectin degradation during sieve pore maturation.
Article
Biochemistry & Molecular Biology
Josip Safran, Vanessa Ung, Julie Bouckaert, Olivier Habrylo, Roland Molinie, Jean-Xavier Fontaine, Adrien Lemaire, Aline Voxeur, Serge Pilard, Corinne Pau-Roblot, Davide Mercadante, Jerome Pelloux, Fabien Senechal
Summary: This study characterized VdPelB, an enzyme from Verticillium dahliae, and found that it exhibited high activity on non-methylesterified pectins, with activity influenced by pH and Ca2+ ions. The released oligogalacturonides (OGs) by VdPelB differed from other characterized Pectate lyases, indicating its unique specificity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Apolonio I. Huerta, Gloria Sancho-Andres, Juan Carlos Montesinos, Javier Silva-Navas, Solene Bassard, Corinne Pau-Roblot, Christopher Kesten, Rudolf Schlechter, Susanne Dora, Temurkhan Ayupov, Jerome Pelloux, Julia Santiago, Clara Sanchez-Rodriguez
Summary: The protein RFO1 is identified as a sensor of changes in the pectin methylation levels in the cell wall, and it plays a crucial role in plant growth and defense against Fusarium oxysporum.