Article
Biochemistry & Molecular Biology
Masakazu Toyoshima, Chiaki Yamamoto, Yoshifumi Ueno, Yoshihiro Toya, Seiji Akimoto, Hiroshi Shimizu
Summary: The study revealed that under specific Red1 light conditions, NDH-1 plays a significant role in the growth of Synechocystis, with its main function being in the electron transport pathway it depends on.
Article
Biotechnology & Applied Microbiology
Kateryna Kukil, Elias Englund, Nick Crang, Elton P. Hudson, Pia Lindberg
Summary: In this study, phenylalanine resistant mutant strains of the unicellular cyanobacterium Synechocystis sp. PCC 6803 were generated by laboratory evolution. The mutant strains were able to secrete phenylalanine and showed potential for production of trans-cinnamic acid and para-coumaric acid. The PRM8 strain demonstrated the highest specific production of these compounds.
METABOLIC ENGINEERING
(2023)
Article
Genetics & Heredity
Chen Xu, Bing Wang, Lin Yang, Lucas Zhongming Hu, Lanxing Yi, Yaxuan Wang, Shenglan Chen, Andrew Emili, Cuihong Wan
Summary: In this study, using co-fractionation coupled with LC-MS/MS, the researchers identified 291 multi-protein complexes and 24,092 protein-protein interactions in Synechocystis sp. PCC 6803. These interactions reveal the roles of photosynthesis in various physiological processes and highlight the differences in protein functions between bacteria and higher plants.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2022)
Article
Biochemical Research Methods
Ian Sofian Yunus, Zhixuan Wang, Pachara Sattayawat, Jonathan Muller, Fessehaye W. Zemichael, Klaus Hellgardt, Patrik R. Jones
Summary: The study focused on improving the productivity of 1-octanol by enhancing enzyme efficiency and optimizing environmental conditions. By adjusting the inducer concentration and light intensity, the researchers were able to increase the 1-octanol productivity significantly, reaching a carbon partitioning rate of 35% and a titer of 526 mg/L within 12 days.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Microbiology
Jin Ding, Nils Schuergers, Heike Baehre, Annegret Wilde
Summary: This study discovered functional CARF-domain effector and ring nuclease proteins in the cyanobacterium Synechocystis sp. PCC 6803. The Csx1 homolog, SyCsx1, is a strict cytosine-specific RNase that depends on cyclic tetraadenylate (cA4). Another CARF-domain protein similar to Csm6 effectors, SyCsm6, does not have RNase activity but can degrade cOAs and attenuate SyCsx1 RNase activity. This research suggests that the CRISPR systems in Synechocystis have a multi-layered cA4-mediated defense mechanism.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Parveen Akhtar, Avratanu Biswas, Fanny Balog-Vig, Ildiko Domonkos, Laszlo Kovacs, Petar H. Lambrev
Summary: Cyanobacterial mutants with monomeric PSI show changes in the composition and abundance of phycobilisomes and in the excitation energy transfer to PSII and PSI. The oligomeric state of PSI impacts the excitation energy flow in Synechocystis.
Article
Plant Sciences
Xiang Li, Chunling Xue, Hui Chen, Huafeng Zhang, Qiang Wang
Summary: This study discovered a small antisense RNA gene (ThfR) that interacts with the thf1 gene, regulating photosynthesis under nitrogen starvation or high-light stress, and enhancing the growth ability of cyanobacteria under high-light stress.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Lucas Gewehr, Benedikt Junglas, Ruven Jilly, Johannes Franz, Wenyu Eva Zhu, Tobias Weidner, Mischa Bonn, Carsten Sachse, Dirk Schneider
Summary: The dynamin-like protein SynDLP in the cyanobacterium Synechocystis sp. PCC 6803 forms ordered oligomers and interacts with negatively charged thylakoid membrane lipids independently of nucleotides. The structural characteristics of SynDLP suggest it to be a bacterial ancestor of eukaryotic dynamin.
NATURE COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Eleftheria-Angeliki Valsami, Angeliki Pateraki, Anastasios Melis, Demetrios F. Ghanotakis
Summary: This study investigated various cultivation conditions for heterologous beta-phellandrene production by Synechocystis sp. PCC 6803, finding that immobilized cells in calcium alginate beads resulted in higher production compared to suspended cultures. Additionally, extreme conditions of pH 10.5 and 600 mM NaCl proved to be conducive for cell growth and beta-phellandrene production, offering new approaches for sustainable production of terpenoids.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Vetaka Tharasirivat, Saowarath Jantaro
Summary: The overexpression of the RuBisCO gene can increase cyanobacterial biomass and improve biocompound production. We successfully constructed two strains of Synechocystis sp. PCC 6803 that overexpressed the rbc gene, resulting in faster growth compared to the wild type strain. The increased biomass of these strains led to higher production of polyhydroxybutyrate (PHB) under nutrient-deprived conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Gang Ruan, Wujuan Mi, Xuwang Yin, Gaofei Song, Yonghong Bi
Summary: This study investigated the physiological response of Synechocystis sp. PCC 6803 to cadmium stress and identified the toxicity targets and response mechanisms. Cadmium was found to damage the cell's photosynthesis and ultrastructure. The study also identified the target sites of cadmium and revealed that the upregulation of the ABC transporter pathway and its protein expression contributed to the response mechanism to cadmium toxicity.
Article
Plant Sciences
Shoki Ito, Atsuko Watanabe, Takashi Osanai
Summary: This study biochemically characterized the L-aspartate oxidase from Synechocystis sp. PCC 6803 and determined its role in NADP+ biosynthesis under photoautotrophic conditions. The results provide important insights into the regulatory mechanism of cyanobacterial NADP+ biosynthesis.
Article
Plant Sciences
Luna Alvarenga-Lucius, Marketa Linhartova, Hendrik Schubert, Sandra Maass, Doerte Becher, Wolfgang R. Hess, Roman Sobotka, Martin Hagemann
Summary: We describe a newly assigned protein comprising 37 amino acids that is encoded upstream of the superoxide dismutase SodB encoding gene. We provide evidence that it fulfills important functions related to the organization of photosynthetic complexes and serves as a molecular glue promoting the aggregation of thylakoid complexes, contributing to different electron transfer modes and energy dissipation under stress conditions.
Article
Biotechnology & Applied Microbiology
Xinyu Hu, Tianyuan Zhang, Kai Ji, Ke Luo, Li Wang, Wenli Chen
Summary: This study investigated the response of Synechocystis sp. PCC 6803 to MV-induced oxidative stress using transcriptomic and metabolomic analyses. Results showed that oxidative stress was strongest at 6 hours, with the highest proportion of upregulated genes and metabolites involved in ten metabolic pathways. Photosynthesis positively regulated the response, while environmental information processing was inhibited by MV.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Sang-Hyeok Cho, Yujin Jeong, Seong-Joo Hong, Hookeun Lee, Hyung-Kyoon Choi, Dong-Myung Kim, Choul-Gyun Lee, Suhyung Cho, Byung-Kwan Cho
Summary: This study elucidated the inhibitory mechanisms of photosynthesis in cyanobacteria under high-light and low-temperature stress conditions, highlighting the differences in response mechanisms between the two stress conditions. Through multiomics data analysis and construction of genome-wide transcription unit architecture, factors affecting photosynthesis at transcription, posttranscription, and translation levels were identified, providing pivotal information for future cyanobacterial cell factories development.
Article
Plant Sciences
Gabor Bernat, Gabor Steinbach, Radek Kana, Govindjee, Amarendra N. Misra, Ondrej Prasil
PHOTOSYNTHESIS RESEARCH
(2018)
Article
Microbiology
Takako Masuda, Gabor Bernat, Martina Beckova, Eva Kotabova, Evelyn Lawrenz, Martin Lukes, Josef Komenda, Ondrej Prasil
ENVIRONMENTAL MICROBIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Thomas Wallner, Yoshinori Hagiwara, Gabor Bernat, Roman Sobotka, Edward J. Reijerse, Nicole Frankenberg-Dinkel, Annegret Wilde
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2012)
Article
Biochemistry & Molecular Biology
Eyal Tamary, Vladimir Kiss, Reinat Nevo, Zach Adam, Gabor Bernat, Sascha Rexroth, Matthias Roegner, Ziv Reich
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2012)
Article
Microbiology
Gabor Bernat, Jens Appel, Teruo Ogawa, Matthias Roegner
JOURNAL OF BACTERIOLOGY
(2011)
Article
Biophysics
Yashar Feyziyev, Zsuzsanna Deak, Stenbjorn Styring, Gabor Bernat
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
(2013)
Article
Biochemistry & Molecular Biology
Igor N. Stadnichuk, Mikhail F. Yanyushin, Gabor Bernat, Dmitry V. Zlenko, Pavel M. Krasilnikov, Evgeny P. Lukashev, Evgeny G. Maksimov, Vladimir Z. Paschenko
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2013)
Article
Plant Sciences
Gabor Bernat, Ulrich Schreiber, Esther Sendtko, Igor N. Stadnichuk, Sascha Rexroth, Matthias Roegner, Friederike Koenig
PLANT AND CELL PHYSIOLOGY
(2012)
Article
Biotechnology & Applied Microbiology
Jong-Hee Kwon, Gabor Bernat, Heiko Wagner, Matthias Roegner, Sascha Rexroth
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2013)
Article
Environmental Sciences
George S. Bullerjahn, Robert Michael L. McKay, Gabor Bernat, Ondrej Prasil, Lajos Voros, Karoly Palffy, Nora Tugyi, Boglarka Somogyi
JOURNAL OF GREAT LAKES RESEARCH
(2020)
Review
Biology
Boglarka Somogyi, Tamas Felfoldi, Laszlo G. Toth, Gabor Bernat, Lajos Voros
Article
Marine & Freshwater Biology
Gabor Bernat, Nora Boross, Boglarka Somogyi, Lajos Voros, Laszlo G-Toth, Gergely Boros
Article
Microbiology
Sophie Rabouille, Douglas A. Campbell, Takako Masuda, Tomas Zavrel, Gabor Bernat, Lubos Polerecky, Kimberly Halsey, Meri Eichner, Eva Kotabova, Susanne Stephan, Martin Lukes, Pascal Claquin, Jose Bonomi-Barufi, Ana Teresa Lombardi, Jan Cerveny, David J. Suggett, Mario Giordano, Jacco C. Kromkamp, Ondrej Prasil
Summary: Marine diazotrophs play key roles in biogeochemical fluxes, but their growth efficiency can be hindered by carbon and nitrogen limitations. The unicellular diazotrophic cyanobacterium Cyanothece faces a double burden of ATP-demanding nitrogen acquisition and metabolic losses when grown in obligate diazotrophy.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Nora Szabo-Tugyi, Lajos Voros, Katalin Balogh, Zoltan Botta-Dukat, Gabor Bernat, Denes Schmera, Boglarka Somogyi
FEMS MICROBIOLOGY ECOLOGY
(2019)