Article
Biochemistry & Molecular Biology
Rui-Lin Xiong, Jiao-Zhen Zhang, Xin-Yan Liu, Jian-Qun Deng, Ting-Ting Zhu, Rong Ni, Hui Tan, Ju-Zheng Sheng, Hong-Xiang Lou, Ai-Xia Cheng
Summary: Liverworts contain bibenzyls and O-glycosides with antioxidant activity. Two bibenzyl UDP-glucosyltransferases, MpUGT737B1 and MpUGT741A1, were identified from the liverwort Marchantia polymorpha. MpUGT741A1 specifically accepted the bibenzyl lunularin as a substrate, while MpUGT737B1 accepted bibenzyls, dihydrochalcone, and phenylpropanoids as substrates and converted phloretin to phloretin-4-O-glucoside and phloridzin. Both enzymes showed selectivity towards UDP-glucose as a sugar donor. The expression levels of these enzymes increased after UV irradiation, suggesting their role in plant stress adaptation. Subcellular localization revealed their presence in the cytoplasm and nucleus. These enzymes have potential for synthesizing bioactive bibenzyl O-glucosides and enhancing plant antioxidant capacity.
Article
Developmental Biology
Naoki Minamino, Takuya Norizuki, Shoji Mano, Kazuo Ebine, Takashi Ueda
Summary: Gametogenesis is a crucial process in sexual reproduction, and the sperm development in bryophytes involves intricate cellular reorganization. This study classified the developmental processes during sperm formation in the liverwort Marchantia polymorpha based on changes in cellular and nuclear shapes, as well as flagellar development. The researchers also investigated the remodeling of microtubules and the reorganization of endomembrane organelles. The findings revealed changes in the glutamylation of tubulin during flagella and spline formation, and the precise and regulated reorganization of plasma membrane and endomembrane organelles involving the functions of endosomal sorting complexes required for transport (ESCRT) machineries. These findings provide valuable insights for the classification of sperm development in bryophytes.
Article
Plant Sciences
Takuya Norizuki, Naoki Minamino, Miyuki Sato, Takashi Ueda
Summary: This study investigates the involvement of autophagy in the degradation and reorganization of plastids during spermiogenesis in the liverwort Marchantia polymorpha. The results show that autophagy-dependent degradation of plastids occurs during spermiogenesis and impaired autophagy leads to defective morphological changes and starch accumulation in the plastid.
FRONTIERS IN PLANT SCIENCE
(2023)
Editorial Material
Cell Biology
Takuya Norizuki, Takashi Ueda
Summary: Sperm mitochondria in bryophytes undergo reorganization through mitochondrial fission and degradation processes. Other cytoplasmic components are also degraded via autophagy, but mitochondria have a distinct degradation time. This study highlights the dynamic reorganization of organelles through multiple degradation pathways during spermiogenesis in M. polymorpha.
Article
Evolutionary Biology
George Sandler, Aneil F. Agrawal, Stephen Wright
Summary: This study examines the genetic diversity of the facultatively sexual liverwort Marchantia polymorpha and finds no evidence of strictly clonal descent or isolation by distance. Instead, evidence of recent recombination and gene flow between geographically distant isolates suggests a modest frequency of sexual reproduction. However, the genome of M. polymorpha has low levels of nucleotide diversity and signs of inefficient selection, indicating possible linked selection and a small effective population size due to asexual propagation. Overall, the genome reveals a complex history of both sexual and asexual reproduction in M. polymorpha.
GENOME BIOLOGY AND EVOLUTION
(2023)
Article
Plant Sciences
Shahin Imran, Masumi Oyama, Rie Horie, Natsuko Kobayashi, Alex Costa, Ryosuke Kumano, Chiho Hirata, Sen Thi Huong Tran, Maki Katsuhara, Keitaro Tanoi, Takayuki Kohchi, Kimitsune Ishizaki, Tomoaki Horie
Summary: This study investigates the structure and function of the high-affinity potassium transporter (MpHKT1) in the liverwort Marchantia polymorpha. The C-terminal hydrophilic domain of MpHKT1 is found to play a unique role in regulating transport activity and ion selectivity. Overexpression of MpHKT1 leads to higher Na+ accumulation, while phenotypes with truncated C-terminal domains are weaker.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Plant Sciences
Yuta Hayashida, Chiaki Yamamoto, Fumio Takahashi, Aika Shibata, Masahiro Kasahara
Summary: The study characterized the PDE domain of the cAMP-synthesis/hydrolysis enzyme CAPE from Marchantia polymorpha, showing that Ca2+ enhances PDE activity and only dipyridamole moderately inhibits PDE activity by approximately 40% at high concentrations.
JOURNAL OF PLANT RESEARCH
(2022)
Article
Plant Sciences
Ulf Lagercrantz, Anja Billhardt, Sabine N. Rousku, Martina Leso, Salim Hossain Reza, D. Magnus Eklund
Summary: Previous studies on the evolution of plant circadian clocks have often focused on genes and models defined in Arabidopsis, but this study took a more unbiased approach by looking at clock genes in liverwort Marchantia polymorpha. The results showed that a homologue to the transcriptional co-repressor Arabidopsis DE-ETIOLATED1 plays an important role in clock gene expression and rhythms, particularly in the liverwort's circadian clock.
Article
Plant Sciences
Erika Bellini, Elena Bandoni, Silvia Giardini, Carlo Sorce, Carmelina Spano, Stefania Bottega, Debora Fontanini, Arian Kola, Daniela Valensin, Andrea Bertolini, Alessandro Saba, Luca Paoli, Andrea Andreucci, Mingai Li, Claudio Varotto, Luigi Sanita di Toppi
Summary: Plants have developed mechanisms to control the uptake and accumulation of both essential and non-essential metals. This includes chelation and sequestration of metals by thiol ligands, such as glutathione and phytochelatins. In the liverwort Marchantia polymorpha, the cell wall moderately reduces the influx of cadmium into cells, and intracellularly, cadmium induces the activity of phytochelatin synthase, leading to synthesis of phytochelatins. Glutathione and phytochelatins chelate cadmium at the cytosolic level, allowing its detoxification.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Cell Biology
Tianhong Li, Li Zhang, Shengzhong Su, Sudi Li, Junchuan Zhang, Zhenming Yang, Zecheng Zuo
Summary: The study found that MpCRY in the liverwort Marchantia polymorpha affects the symmetrical growth of thalli under light conditions, primarily involved in photosynthesis and sugar metabolism. Additionally, exogenous application of sucrose or glucose partially restored the symmetrical growth of thalli.
Article
Biochemistry & Molecular Biology
Mateusz Stelmasiewicz, Lukasz Swiatek, Agnieszka Ludwiczuk
Summary: Liverwort endophytes, particularly those from spore-forming plants, could be a valuable source of new biologically active substances not found in other organisms. In this study, volatile compounds obtained from Marchantia polymorpha L. liverwort and its endophytes were analyzed. Diketopiperazine compounds were identified in the endophyte extract, while the liverwort volatile extract contained sesquiterpenoids of cuparane, chamigrane, acorane, and thujopsane types. The ethyl acetate extracts from the endophytic microorganisms showed significant anticancer potential against hypopharyngeal squamous cell carcinoma and cervical adenocarcinoma.
Article
Plant Sciences
Kenta C. C. Moriya, Makoto Shirakawa, Jeanne Loue-Manifel, Yoriko Matsuda, Yen-Ting Lu, Kentaro Tamura, Yoshito Oka, Tomonao Matsushita, Ikuko Hara-Nishimura, Gwyneth Ingram, Ryuichi Nishihama, Justin Goodrich, Takayuki Kohchi, Tomoo Shimada
Summary: The acquisition of new gene regulatory networks controlled by transcription factors is necessary for the evolution of special types of cells. In this study, it was found that a module formed by subfamilies Ia and IIIb basic helix-loop-helix transcription factors regulates the development of a unique sporophyte tissue, the seta, in the astomatous liverwort Marchantia polymorpha. This module also partially replaces the stomatal function in Arabidopsis thaliana, implying a common regulatory mechanism underlying setal and stomatal formation.
Article
Plant Sciences
Shogo Kawamura, Facundo Romani, Masaru Yagura, Takako Mochizuki, Mika Sakamoto, Shohei Yamaoka, Ryuichi Nishihama, Yasukazu Nakamura, Katsuyuki T. Yamato, John L. Bowman, Takayuki Kohchi, Yasuhiro Tanizawa
Summary: In this study, a web-based suite of tools, MBEX, was developed to analyze and summarize transcriptome data of Marchantia polymorpha. Using oil body biogenesis as an example, the results generated by MBEX were consistent with experimental evidence and revealed a novel transcriptional network.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Plant Sciences
Katharina Melkonian, Sara Christina Stolze, Anne Harzen, Hirofumi Nakagami
Summary: The miniTurbo-based interactomics method has been successfully applied in M. polymorpha, yielding a manifold of potential interactors of MpSYP12A and MpSYP13B and providing a useful resource for elucidating the evolution of SNARE functions.
Article
Biochemistry & Molecular Biology
Li Zhang, Tianhong Li, Shengzhong Su, Hao Peng, Sudi Li, Ke Li, Luyao Ji, Yaoyun Xing, Junchuan Zhang, Xinglin Du, Mingdi Bian, Yuying Liao, Zhenming Yang, Zecheng Zuo
Summary: This study found that the ubiquitination of the photo-responsive transcription factor HY5 by the COP1/SPA complex is conserved in the liverwort Marchantia polymorpha, but unlike in Arabidopsis, the blue light receptor CRY does not inhibit this process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Richalynn Leong, Javier Jingheng Tan, Sally Shuxian Koh, Ting-Ying Wu, Kimitsune Ishizaki, Daisuke Urano
Summary: This study investigated the cellular mechanisms of calcium deficiency in plants using the bryophyte Marchantia polymorpha as a model. The results revealed metabolic compounds and genetic pathways involved in the occurrence and expansion of foliar tipburn. Furthermore, comparable changes in gene expression were observed in other plant species, suggesting the conservation of these pathways.
Article
Biochemistry & Molecular Biology
Ayaka Ueda, Yusuke Aihara, Shinya Sato, Keiko Kano, Emi Mishiro-Sato, Hiroyuki Kitano, Ayato Sato, Kazuhiro J. Fujimoto, Takeshi Yanai, Kazuma Amaike, Toshinori Kinoshita, Kenichiro Itami
Summary: This study discovered 2,6-dihalopurines as a new inhibitor of stomatal opening and investigated its mechanism. Biological assays suggested that the pathway related to plasma membrane H+-ATPase phosphorylation may be involved. Through affinity-based pull down assay and molecular dynamics simulation, Leucine-rich repeat extensin proteins (LRXs), LRX3, LRX4, LRX5, and RALF were identified as target protein candidates for 2,6-dihalopurines. The mechanism of stomatal movement related to LRXs-RALF is an unexplored pathway, and further studies may uncover new signaling pathways and regulatory factors in stomatal movement.
ACS CHEMICAL BIOLOGY
(2023)
Article
Plant Sciences
Ming Ding, Yiyong Zhu, Toshinori Kinoshita
Summary: Cauline leaves in Arabidopsis and flag leaves in rice have important roles in supplying photosynthetic products and increasing grain yield. They have higher stomatal conductance and CO2 assimilation rate, which is caused by increased plasma membrane H+-ATPase activity. Removing these leaves significantly reduces plant biomass and seed production in Arabidopsis, as well as grain yield in rice.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Satoru N. Kinoshita, Takamasa Suzuki, Takatoshi Kiba, Hitoshi Sakakibara, Toshinori Kinoshita
Summary: Plant growth and development rely on plasma membrane proton-translocating adenosine triphosphatase (H+-ATPase), which is activated by phosphorylation. Small Auxin-Up RNA proteins regulate the phosphorylation level by inhibiting dephosphorylation. Photosynthetically active radiation activates H+-ATPase, and phosphorylation depends on photosynthesis and sugar supplementation. The molecular mechanism and physiological role of this activation are still unknown.
PLANT AND CELL PHYSIOLOGY
(2023)
Editorial Material
Plant Sciences
Agepati S. Raghavendra, Wenxiu Ye, Toshinori Kinoshita
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Nobiza Khatun, Akihisa Shinozawa, Kosaku Takahashi, Hideyuki Matsuura, Akida Jahan, Mousona Islam, Masudul Karim, Sk Rahul, Mikako Yoshikawa, Kimitsune Ishizaki, Yoichi Sakata, Daisuke Takezawa
Summary: Low-molecular-weight sugars and the stress hormone abscisic acid (ABA) play important roles in protecting cellular membranes and macromolecules during dehydration. ABA also regulates the positive feedback loop for sugar-induced sugar accumulation in liverworts, enhancing desiccation tolerance.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Plant Sciences
Aoi Narutaki, Prihardi Kahar, Shunji Shimadzu, Shota Maeda, Tomoyuki Furuya, Kimitsune Ishizaki, Hidehiro Fukaki, Chiaki Ogino, Yuki Kondo
Summary: In this study, the effects of sugars on vascular cell differentiation were examined using a vascular cell induction system. Sucrose was found to have the strongest inhibitory effect on xylem differentiation. Transcriptome analysis revealed that sucrose suppresses xylem and phloem differentiation in cambial cells. The central regulator BRI1-EMS-SUPPRESSOR1 transcription factor was suggested to be involved in the sucrose-mediated regulation of vascular cell differentiation.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Plant Sciences
Momoko Takagi, Rikako Hirata, Yusuke Aihara, Yuki Hayashi, Miya Mizutani-Aihara, Eigo Ando, Megumi Yoshimura-Kono, Masakazu Tomiyama, Toshinori Kinoshita, Akira Mine, Yosuke Toda
Summary: This paper proposes an image analysis pipeline that can automatically quantify the stomatal aperture of Arabidopsis thaliana leaves. By combining a YOLO X-based stomatal detection submodule and a U-Net-based pore segmentation submodule, high-precision detection and segmentation of stomata and pores are achieved. In addition, a portable imaging device is designed for easy acquisition of stomatal images on-site. Utilizing this device and the optimized models, it is possible to replace manual measurement of stomatal response to pathogen inoculation, accelerating research on stomatal biology of model dicots.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Plant Sciences
Miki Hatada, Ryota Akiyama, Moeko Yamagishi, Kimitsune Ishizaki, Masaharu Mizutani
Summary: This study analyzed the sterol profiles in the bryophyte model plant Marchantia polymorpha L. and identified the presence of sterol biosynthetic genes. Functional analysis revealed the importance of one of these genes for the normal growth and development of the plant.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Plant Sciences
Juntaro Negi, Tomoki Obata, Sakura Nishimura, Boseok Song, Sho Yamagaki, Yuhei Ono, Makoto Okabe, Natsumi Hoshino, Kohei Fukatsu, Ryo Tabata, Katsushi Yamaguchi, Shuji Shigenobu, Masashi Yamada, Mitsuyasu Hasebe, Shinichiro Sawa, Toshinori Kinoshita, Ikuo Nishida, Koh Iba
Summary: A mutant of Arabidopsis, cdi4, was found to have impaired stomatal responses to CO2 and light. This mutation is associated with a gene called PECT1, which encodes CTP:phosphorylethanolamine cytidylyltransferase. By suppressing the expression of PECT1 in guard cells, it was discovered that these mutant lines also exhibited impaired stomatal responses to low-CO2 concentrations or light.
Article
Plant Sciences
Wenxiang Xie, Shuo Liu, Huiling Gao, Jun Wu, Dilin Liu, Toshinori Kinoshita, Chao-Feng Huang
Summary: In rice, the PM-localized PP2C.D phosphatase SAL1 inhibits PM H+-ATPase to regulate NRAT1 transporter-mediated Al uptake and Al resistance. Aluminum toxicity remains a primary constraint for crop production in acidic soils. The molecular mechanisms underlying high Al resistance in rice and the strategies used by rice and Arabidopsis to defend against Al toxicity are revealed.
Article
Multidisciplinary Sciences
Yusuke Aihara, Bumpei Maeda, Kanna Goto, Koji Takahashi, Mika Nomoto, Shigeo Toh, Wenxiu Ye, Yosuke Toda, Mami Uchida, Eri Asai, Yasuomi Tada, Kenichiro Itami, Ayato Sato, Kei Murakami, Toshinori Kinoshita
Summary: The authors identify BITC as a stomatal opening inhibitor that suppresses PM H+-ATPase phosphorylation. They develop BITC derivatives with higher inhibitory activity that increase drought tolerance in plants.
NATURE COMMUNICATIONS
(2023)
Article
Plant Sciences
Chieko Goto, Akira Ikegami, Tatsuaki Goh, Kaisei Maruyama, Hiroyuki Kasahara, Yumiko Takebayashi, Yuji Kamiya, Koichi Toyokura, Yuki Kondo, Kimitsune Ishizaki, Tetsuro Mimura, Hidehiro Fukaki
Summary: In Arabidopsis thaliana, the mutation in the GNOM gene severely inhibits lateral root (LR) formation, while the fewer roots suppressor1 (fsp1) mutation partially restores LR formation. It has been found that fsp1 mutation increases endogenous IAA levels and the number of sites where auxin response accumulates before LR formation, and SUR2 gene affects IAA accumulation in the pericycle cells at the xylem pole.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Plant Sciences
Misao Shimojo, Masashi Nakamura, Ginga Kitaura, Yuta Ihara, Shinsuke Shimizu, Koichi Hori, Masako Iwai, Hiroyuki Ohta, Kimitsune Ishizaki, Mie Shimojima
Summary: This study analyzed the impact of membrane lipid remodeling on inorganic phosphate uptake and discovered the crucial role of MpPAH in the growth of Marchantia polymorpha. The mutation of MpPAH affects plant growth by suppressing the synthesis of plastid glycolipids through the endoplasmic reticulum pathway.