Article
Plant Sciences
Shuai Liu, Hua Zhong, Qiang Wang, Caixiang Liu, Ting Li, Zhaohua Peng, Yangsheng Li, Hongyu Zhang, Jianglin Liao, Yingjin Huang, Zhaohai Wang
Summary: This study analyzed 454 UDP glucose pyrophosphorylase (UDPGP) proteins from 76 different species, classifying them into three clades based on phylogenetic tree and orthogroups. The study revealed a conserved evolutionary history and functional characteristics of UDPGP genes.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Microbiology
Oriana Villafraz, Helene Baudouin, Muriel Mazet, Hanna Kulyk, Jean-William Dupuy, Erika Pineda, Cyrille Botte, Daniel Ken Inaoka, Jean-Charles Portais, Frederic Bringaud
Summary: Trypanosomatids sequester glycolysis inside peroxisomes called glycosomes and have functional sugar nucleotide biosynthetic pathways in addition to cytosolic ones. The enzyme UDP-glucose pyrophosphorylase (UGP) is imported into glycosomes by piggybacking on phosphoenolpyruvate carboxykinase (PEPCK) without a known peroxisome-targeting signal, suggesting a unique mechanism for protein import. These findings shed light on the unconventional compartmentalization of metabolic pathways in trypanosomatids.
Article
Plant Sciences
Martina Althammer, Christof Regl, Klaus Herburger, Constantin Blochl, Elena Voglas, Christian G. Huber, Raimund Tenhaken
Summary: Galactose toxicity (Gal-Tox) is a common phenomenon in plants, but little is known about the mechanism behind it. This study investigated the role of galactose-containing metabolites like galactose-1-phosphate (Gal-1P) and UDP-Gal in Gal-Tox. Overexpressing USP from Pisum sativum helped reduce Gal-1P levels and alleviate Gal-Tox symptoms in roots. However, the overexpression lines became more sensitive to exogenous galactose. This could be due to the elevated levels of UDP-Gal in the roots.
Article
Multidisciplinary Sciences
Tuan D. Tran, Munira Aman Ali, Davin Lee, Marie-Anne Felix, Robert J. Luallen
Summary: The study reveals the mechanism by which an intracellular bacterium spreads between intestinal epithelial cells of a natural host by forming filaments. This process is regulated by a conserved nutrient-sensing pathway and reduces host lifespan and fecundity.
NATURE COMMUNICATIONS
(2022)
Article
Plant Sciences
Rahele Panahabadi, Asadollah Ahmadikhah, Lauren S. McKee, Par K. Ingvarsson, Naser Farrokhi
Summary: The genetic variability of cellulose, lignin, and fermentable sugars contents in rice straw was analyzed through a genome-wide association study, identifying genomic regions significantly associated with these components. Candidate genes involved in cell wall metabolism were found to be associated with cellulose, lignin, and fermentable sugar contents. New SNP markers associated with these components were identified, aiding in understanding the genetic basis of biosynthesis, turnover, and modification of major cell wall components and saccharides in rice straw.
Article
Chemistry, Multidisciplinary
Jin-Min Shi, Ting-Ting Wu, Hui Zhou, Yao-Yao Zhang, Li Liu, Goran Widmalm, Josef Voglmeir
Summary: Galactokinases (GalKs) are a group of structurally conserved proteins found in all life forms. A unique GalK orthologue from the bacterium Solitalea canadensis (ScGalK) has been discovered with relaxed substrate requirements and the ability to convert xylose to xylose-1-phosphate. Further investigation identified a putative Glucose-1-phosphate Uridyltransferase (ScGPUT) in close proximity to the ScGalK gene, which can convert xylose-1-phosphate to uridine diphosphate xylose (UDP-xylose) with the presence of uridine triphosphate (UTP).
Article
Plant Sciences
Zhaohai Wang, Qiang Wang, Lingxia Wei, Yan Shi, Ting Li, KeKe Hu, Shuai Liu, Hua Zhong, Jianglin Liao, Yangsheng Li, Hongyu Zhang, Yingjin Huang
Summary: Functional inactivation of UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) induces defense response-related lesion-mimic spots and early senescence in every newly grown leaf of the rice mutant uap1 after a short period's normal growth. However, the molecular mechanism of these leaves sustaining the short period's survival remains unknown. Both UAP1 and UAP2 proteins have highly similar enzymatic activity and subcellular localization, with UAP2 playing a crucial role in maintaining initial normal leaf growth in uap1 plants. Overexpression of the UAP2 gene in uap1 plants rescues the leaf mutant phenotype, indicating the functional similarity between UAP1 and UAP2 in protecting rice leaves from early senescence.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Multidisciplinary Sciences
Zhongyang Xu, Jiasen He, Muhammad Tehseen Azhar, Zhen Zhang, Senmiao Fan, Xiao Jiang, Tingting Jia, Haihong Shang, Youlu Yuan
Summary: This study identified 66 UDP-glucose pyrophosphorylase (UGP) genes from four cotton species, which play an important role in cotton fiber development. The analysis of evolutionary relationship, gene structure, and expression revealed the conservation and function of UGP genes in cotton fiber development.
Article
Microbiology
Benjamin Sellner, Ruta Prakapaite, Margo van Berkum, Matthias Heinemann, Alexander Harms, Urs Jenal
Summary: Bacteriophage N4 uses a novel surface glycan as a receptor to infect E. coli, with the process regulated by the second messenger c-di-GMP and specific enzymes DgcJ and PdeL. The protection mechanism mediated by PdeL involves reducing c-di-GMP and repressing the wec operon, which serves as a receptor for N4.
Article
Biochemistry & Molecular Biology
Qing Chen, Xunju Liu, Yueyang Hu, Yan Wang, Bo Sun, Tao Chen, Ya Luo, Yong Zhang, Mengyao Li, Zejing Liu, Xiaorong Wang, Haoru Tang
Summary: In this study, cloning and functional characterization of a UGT, UGT78H2 in blackberry fruits was reported. The UGT78H2 was found to predominantly glycosylate quercetin and exhibit higher catalytic efficiency towards UDP-galactose.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Multidisciplinary Sciences
Chenxi Qiu, Huilin Tao, Yu Shen, Qingsheng Qi, Jin Hou
Summary: Regulating storage carbohydrate synthesis improves the availability of acetyl-CoA and malonyl-CoA, facilitating the synthesis of their derived chemicals.
Article
Plant Sciences
Sandeep Jaiswal, Krishnayan Paul, K. Venkat Raman, Saurabh Tyagi, Manjesh Saakre, Jyotsana Tilgam, Sougata Bhattacharjee, Joshitha Vijayan, Kalyan Kumar Mondal, Rohini Sreevathsa, Debasis Pattanayak
Summary: RNAi-mediated downregulation of the StUGPase gene expression level in potato can reduce the accumulation of reducing sugars during cold storage, leading to the development of CIS tolerant potato varieties.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Pharmacology & Pharmacy
Christian Bailly, Gerard Vergoten
Summary: The synthetic antibacterial drug clofoctol has been widely used in Europe to treat respiratory tract infections and has been found to exhibit anti-tumor activity by targeting two important proteins. The drug-protein binding models can guide the design of more potent anticancer compounds. Therefore, there is a renewed interest in clofoctol for the treatment of cancers and potentially COVID-19.
DRUG DISCOVERY TODAY
(2021)
Review
Chemistry, Medicinal
Luca Gallelli, Erika Cione, Tian Wang, Leiming Zhang
Summary: Escin, a natural compound, exerts therapeutic effects mainly by relieving tissue edema, promoting venous drainage, and reducing inflammation. Studies suggest that escin may exert its anti-inflammatory and anti-edematous effects through a glucocorticoid-like activity without causing glucocorticoid-like adverse drug reactions.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2021)
Article
Chemistry, Applied
Ana Carolina Gomes, Alexandre Borges, Daniela Garcia Zoca, Marcio Luis Andrade e Silva, Aline Rafaela da Silva Rodrigues Machado, Alex Martins Machado, Mario F. C. Santos, Rosangela da Silva de Laurentiz
Summary: This study evaluated the larvicidal potential of Piper cubeba extracts and compounds against Aedes aegypti larvae, the primary vector of dengue, yellow fever, chikungunya, and zika viruses. The results demonstrated the larvicidal action of hinokinin and extracts, suggesting their potential for the development of new natural larvicides. Scanning electron microscopy analysis revealed structural damage to the larvae's tegument caused by the extracts and compounds.
NATURAL PRODUCT RESEARCH
(2023)
Article
Plant Sciences
Abir U. Igamberdiev, Leszek A. Kleczkowski
FRONTIERS IN PLANT SCIENCE
(2015)
Article
Plant Sciences
Sabine Kunz, Per Gardestrom, Edouard Pesquet, Leszek A. Kleczkowski
FRONTIERS IN PLANT SCIENCE
(2015)
Review
Plant Sciences
Olivier Keech, Per Gardestrom, Leszek A. Kleczkowski, Nicolas Rouhier
PLANT CELL AND ENVIRONMENT
(2017)
Article
Plant Sciences
Joanna Lesniewska, David Ohman, Magdalena Krzeslowska, Sunita Kushwah, Maria Barciszewska-Pacak, Leszek A. Kleczkowski, Bjorn Sundberg, Thomas Moritz, Ewa J. Mellerowicz
Article
Plant Sciences
Daniel Decker, Christopher Oberg, Leszek A. Kleczkowski
Article
Plant Sciences
Daniel Decker, Leszek A. Kleczkowski
FRONTIERS IN PLANT SCIENCE
(2017)
Review
Plant Sciences
Abir U. Igamberdiev, Leszek A. Kleczkowski
FRONTIERS IN PLANT SCIENCE
(2018)
Correction
Plant Sciences
Abir U. Igamberdiev, Leszek A. Kleczkowski
FRONTIERS IN PLANT SCIENCE
(2018)
Article
Chemistry, Analytical
Daniel Decker, Stina Lindberg, Jonas Eriksson, Leszek A. Kleczkowski
ANALYTICAL METHODS
(2014)
Article
Multidisciplinary Sciences
Sabine Kunz, Edouard Pesquet, Leszek A. Kleczkowski
Article
Biochemistry & Molecular Biology
Leszek A. Kleczkowski, Abir U. Igamberdiev
BIOCHEMICAL JOURNAL
(2020)
Review
Biochemistry & Molecular Biology
Leszek A. Kleczkowski, Abir U. Igamberdiev
Summary: Free magnesium (Mg2+) functions as a signal of adenylate (ATP+ADP+AMP) status in cells, controlled by the equilibrium of adenylate kinase (AK) and membrane-bound adenylate- and Mg2+-translocators. Changes in [Mg2+] impact enzyme activities, metabolism, and processes like photosynthesis, under normal and stress conditions. Magnesium signaling is likely widespread in all eukaryotic and prokaryotic organisms due to the ubiquitous nature of AK and its role in adenylate equilibration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Abir U. Igamberdiev, Leszek A. Kleczkowski
Summary: Under conditions of low oxygen stress, pyrophosphate (PPi) can serve as an alternative energy currency in plant cells, ensuring the continuous operation of glycolysis in the absence of ATP synthesis.
BIOCHEMICAL JOURNAL
(2021)
Article
Plant Sciences
Leszek A. Kleczkowski, Daniel Decker
Summary: UDP-glucose pyrophosphorylase (UGPase) is an essential enzyme in glycosylation reactions, and this study reveals the significant role of free magnesium ions in its reverse reaction. The MgPPi complex serves as the true substrate for UGPase, while free pyrophosphate inhibits the reaction. Phosphonates, analogs of pyrophosphate and phosphate, showed different effects on UGPase activity.
Article
Biochemistry & Molecular Biology
Daniel Decker, Christopher Oberg, Leszek A. Kleczkowski
PLANT SIGNALING & BEHAVIOR
(2018)
