Article
Plant Sciences
Z-C Yu, Y-N Luo, W-Y Shi, W. Lin, C-L Peng
Summary: In subtropical forests, young leaves of Castanopsis chinensis have higher anthocyanin content and related gene expression compared to Machilus chinensis, while the latter shows higher levels of antioxidants and nonphotochemical quenching in young leaves without anthocyanin accumulation. This study suggests that anthocyanins, antioxidants, and nonphotochemical quenching collectively play a positive role in photoadaptation of young leaves.
Article
Plant Sciences
Anirban Guha, Talent Vharachumu, Muhammad F. Khalid, Mark Keeley, Thomas J. Avenson, Christopher Vincent
Summary: This study using citrus as a model plant sheds light on the basic mechanisms of photosynthetic adaptation in tropical evergreen species, revealing significant genotypic differences in photosynthetic thermotolerance and different responses to short-term warming and postwarming events.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Plant Sciences
Thekla von Bismarck, Kuebra Korkmaz, Jeremy Russ, Kira Skurk, Elias Kaiser, Viviana Correa Galvis, Jeffrey A. Cruz, Deserah D. Strand, Karin Koehl, Juergen Eirich, Iris Finkemeier, Peter Jahns, David M. Kramer, Ute Armbruster
Summary: Understanding photosynthesis in natural light environments requires knowledge of the long-term acclimation as well as the short-term responses and their interactions. This study investigates the effects of light intensity and variability on thylakoid ion transport, which plays a crucial role in dynamic photosynthesis. The findings reveal that daily light intensity shapes photosynthetic capacity and fluctuations in light intensity enhance the accumulation of protective pigment zeaxanthin. The study also uncovers the role of ion transporters in regulating zeaxanthin accumulation and facilitating the transition to lower light intensities.
Article
Multidisciplinary Sciences
Giorgio Perin, Alessandra Bellan, Tim Michelberger, Dagmar Lyska, Setsuko Wakao, Krishna K. Niyogi, Tomas Morosinotto
Summary: Life on earth relies on photosynthetic primary producers, including microalgae, to convert sunlight and CO2 into biomass. Microalgae have the potential to be a valuable biomass source and contribute to a sustainable bioeconomy. This study investigates the role of the xanthophyll cycle in protecting microalgae from excess light and optimizing biomass productivity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Plant Sciences
Marguerite Cinq-Mars, Guy Samson
Summary: This study revealed that the decline in CO2 assimilation at frequencies of 10 Hz and lower was associated with the formation of nonphotochemical quenching (NPQ) and a restriction of electron flows toward PSI. The results suggest that under these conditions, there is a favoring of accumulation of harmless P-700(+) at the expense of decreased quantum yield of photochemistry for PSII.
Article
Engineering, Environmental
Xiaofeng Liu, Aris P. Georgakakos
Summary: This study found that developing a bias correction method can lead to more accurate measurements of Chl-a concentration in freshwater lakes and reservoirs, improving the overall accuracy of current methods.
Article
Biology
Renan Falcioni, Werner Camargos Antunes, Jose A. M. Dematte, Marcos Rafael Nanni
Summary: The study investigates the use of chlorophyll a fluorescence kinetics analyses and reflectance hyperspectroscopy to monitor the photosynthetic process in Codiaeum variegatum (L.) A. Juss. The results show that certain vegetation indexes are highly correlated with morphological and pigment parameters, while others are associated with photochemical components of photosynthesis. These findings are significant for monitoring nonuniform leaves, especially those with high pigment profiling variations.
Article
Biochemistry & Molecular Biology
Mahmoud Sharawy, Natalia B. Pigni, Eric R. May, Jose A. Gascon
Summary: This study investigates the protein rearrangements of the orange carotenoid protein (OCP) using metadynamics. The results reveal the importance of ketocarotenoid translocation for domain separation and provide an energetically feasible pathway to dissociation.
Article
Plant Sciences
Collin J. Steen, Adrien Burlacot, Audrey H. Short, Krishna K. Niyogi, Graham R. Fleming
Summary: This study monitored non-photochemical quenching (NPQ) in intact cells throughout high light/dark cycles of various illumination periods and found that the dynamics of NPQ depend on the timescales of light fluctuations. LHCSR proteins play a major role during the light phases, while state transition activation contributes to NPQ response during dark phases.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Plant Sciences
Claire Seydoux, Mattia Storti, Vasco Giovagnetti, Anna Matuszynska, Erika Guglielmino, Xue Zhao, Cecile Giustini, Yufang Pan, Lander Blommaert, Jhoanell Angulo, Alexander Ruban, Hanhua Hu, Benjamin Bailleul, Florence Courtois, Guillaume Allorent, Giovanni Finazzi
Summary: Diatoms, successful phytoplankton clades, have the ability to adapt to changing light conditions due to their regulatory circuit involving the H+/K+ antiporter KEA3 and diadinoxanthin de-epoxidase. This circuit allows diatoms to efficiently respond to fast or slow changes in light environment, contributing to their thriving in different ocean provinces.
Article
Plant Sciences
Norma Valeria Ahumada-Fierro, Ernesto Garcia-Mendoza, Jose Miguel Sandoval-Gil, Christine Johanna Band-Schmidt
Summary: The study found that the toxic potential of Chattonella is associated with high ROS production, possibly due to a lack of effective photoprotection mechanisms. The three strains of Chattonella exhibited different NPQ and XC characteristics under light conditions, with ROS production related to XC-related thermal dissipation.
JOURNAL OF PHYCOLOGY
(2021)
Article
Biochemical Research Methods
Jianjun Du, Bo Li, Xianju Lu, Xiaozeng Yang, Xinyu Guo, Chunjiang Zhao
Summary: The study developed an automated phenotyping pipeline to recognize the components of detached lettuce leaves and calculate multi-semantic traits for phenotype identification. It constructed six semantic segmentation models to extract leaf components and utilized a leaf normalization technique for consistent leaf phenotyping. The proposed pipeline contributed various geometry-, venation-, and color-based traits for characterizing each lettuce leaf. The method was validated through manual measurements and demonstrated high accuracy for trait identification.
Article
Biochemical Research Methods
Kate Harline, Adrienne H. K. Roeder
Summary: The study presents a simple method for live imaging of Arabidopsis thaliana first leaves. By dissecting the cotyledons, affixing a coverslip, and using perfluorodecalin, optimal imaging series for cellular and organ level analysis can be obtained. The researchers also developed image processing software and scripts for automated analysis and data presentation.
Review
Plant Sciences
Andrew J. Simkin, Leepica Kapoor, C. George Priya Doss, Tanja A. Hofmann, Tracy Lawson, Siva Ramamoorthy
Summary: Photosynthetic pigments, including chlorophyll, carotenoids, and phycobilins, are essential for efficient light absorption and adaptation to different environments in photosynthetic organisms. They play crucial roles in light harvesting, photoprotection, and deep water colonization.
PHOTOSYNTHESIS RESEARCH
(2022)
Article
Plant Sciences
Judy G. Schmalstig, Kenneth Jainandan
Summary: Green light can reduce blue-light-induced chloroplast avoidance movement, but only when presented simultaneously with blue light, while green light alone has no effect on chloroplast position. The action spectrum of green light in duckweed reveals peaks at 510, 550, and 590 nm, and some Arabidopsis mutants are not affected by green light.
AMERICAN JOURNAL OF BOTANY
(2021)
Article
Chemistry, Physical
Hoang Long Nguyen, Thanh Nhut Do, Parveen Akhtar, Thomas L. C. Jansen, Jasper Knoester, Wenda Wang, Jian-Ren Shen, Petar H. Lambrev, Howe-Siang Tan
Summary: The marine green macroalga Bryopsis corticulans has unique light-harvesting complexes compared to those in plants, as revealed by fitting analysis of its LHCII. The fitting method's robustness in matching experimental data demonstrates its effectiveness in systems lacking precise pigment assignment and spatial information.
JOURNAL OF PHYSICAL CHEMISTRY B
(2021)
Correction
Multidisciplinary Sciences
Pushan Bag, Volha Chukhutsina, Zishan Zhang, Suman Paul, Alexander G. Ivanov, Tatyana Shutova, Roberta Croce, Alfred R. Holzwarth, Stefan Jansson
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Marijonas Tutkus, Jevgenij Chmeliov, Gediminas Trinkunas, Parveen Akhtar, Petar H. Lambrev, Leonas Valkunas
Summary: This study investigated the fluorescence properties of liposome-reconstituted major light-harvesting complexes of plants (LHCII) using fluorescent dye labelling and single-molecule microscopy techniques. Results showed that the fluorescence lifetimes in bulk and single liposome measurements were correlated, with shorter lifetimes observed in liposome-free LHCII compared to reconstituted LHCII. The dependence of fluorescence lifetime on protein density in liposome-reconstituted LHCII resembled concentration quenching, while the impact of liposome size on fluorescence lifetime was not significant. It was demonstrated that fluorescence quenching in reconstituted membranes could be induced by LHCII-LHCII interactions, similar to photoprotective non-photochemical quenching in vivo.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Article
Astronomy & Astrophysics
C. H. Lucas Patty, Jonas G. Kuehn, Petar H. Lambrev, Stefano Spadaccia, H. Jens Hoeijmakers, Christoph Keller, Willeke Mulder, Vidhya Pallichadath, Olivier Poch, Frans Snik, Daphne M. Stam, Antoine Pommerol, Brice-Olivier Demory
Summary: This study demonstrates that sensitive circular spectropolarimetric measurements taken from a fast-moving helicopter can quickly differentiate between various landscape elements, including grass fields, forests, and urban areas. Circular polarization can serve as a powerful biosignature and a valuable tool in remote sensing for monitoring vegetation physiology and terrain features.
ASTRONOMY & ASTROPHYSICS
(2021)
Article
Chemistry, Physical
Parveen Akhtar, Gabor Sipka, Wenhui Han, Xingyue Li, Guangye Han, Jian-Ren Shen, Gyozo Garab, Howe-Siang Tan, Petar H. Lambrev
Summary: This study investigates the excitation dynamics of Photosystem II (PSII) under neutral and oxidized reaction center (RC) conditions using femtosecond transient absorption spectroscopy. The core antenna equilibration process has a lifetime of about 300 fs, regardless of the RC redox state. Additionally, slower energy equilibration is observed on timescales of 3-5 ps. PSII with neutral RC follows previously proposed models, while PSII with oxidized RC exhibits extremely fast excitation quenching.
JOURNAL OF CHEMICAL PHYSICS
(2022)
Article
Chemistry, Physical
Thanh Nhut Do, Hoang Long Nguyen, Parveen Akhtar, Kai Zhong, Thomas L. C. Jansen, Jasper Knoester, Stefano Caffarri, Petar H. Lambrev, Howe-Siang Tan
Summary: The study investigates the ultrafast excitation energy transfer processes in the LHCII(M)-CP29-CP24 complex of photosystem II, showing faster energy equilibrations and shorter bottleneck state lifetimes compared to LHCII. Structural changes in LHCII(M) in the bigger assembly are attributed to the accelerated dynamics observed.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Plant Sciences
M. Magyar, P. Akhtar, G. Sipka, W. Han, X. LI, G. Han, J. -R. Shen, P. H. Lambrev, G. Garab
Summary: The process of dark-to-light transition in PSII depends significantly on the lipid matrix of the reaction center. Non-native lipids have no noticeable effects, while thylakoid membrane lipids can considerably shorten the response time of the reaction center.
Article
Plant Sciences
Parveen Akhtar, Avratanu Biswas, Fanny Balog-Vig, Ildiko Domonkos, Laszlo Kovacs, Petar H. Lambrev
Summary: Cyanobacterial mutants with monomeric PSI show changes in the composition and abundance of phycobilisomes and in the excitation energy transfer to PSII and PSI. The oligomeric state of PSI impacts the excitation energy flow in Synechocystis.
Article
Biochemistry & Molecular Biology
Melinda Magyar, Gabor Sipka, Wenhui Han, Xingyue Li, Guangye Han, Jian-Ren Shen, Petar H. H. Lambrev, Gyozo Garab
Summary: By measuring the chlorophyll-a fluorescence transients produced by single-turnover saturating flashes, researchers identified the rate-limiting steps in the transition from dark to light of Photosystem II. They found that in samples treated with diuron, the first flash fully reduced the Q(A) quinone acceptor molecule but only generated an F-1 fluorescence transient.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Physical
Vesna Eric, Xinmeng Li, Lolita Dsouza, Sean K. Frehan, Annemarie Huijser, Alfred R. . Holzwarth, Francesco Buda, G. J. Agur Sevink, Huub J. M. de Groot, Thomas L. C. Jansen
Summary: In this study, the microscopic sources of disorder causing spectral width in chlorosomes are identified. It is found that hydrogen bonding motifs are essential for an accurate description of the spectral line shape and that exciton delocalization is consistent with the two-dimensional electronic spectra.
JOURNAL OF PHYSICAL CHEMISTRY B
(2023)
Review
Biochemistry & Molecular Biology
G. Sipka, L. Nagy, M. Magyar, P. Akhtar, J. -r. Shen, A. R. Holzwarth, P. H. Lambrev, G. Garab
Summary: This review outlines the nature, mechanism, and physiological significance of light-induced reversible reorganizations in closed Type II reaction center complexes. Closed reaction center complexes display changes in their photophysical and photochemical parameters upon continued excitation, leading to the optimization of photosynthetic machinery during the dark-to-light transition.
Article
Chemistry, Physical
Vesna Eric, Jorge Luis Castro, Xinmeng Li, Lolita Dsouza, Sean K. Frehan, Annemarie Huijser, Alfred R. Holzwarth, Francesco Buda, G. J. Agur Sevink, Huub J. M. de Groot, Thomas L. C. Jansen
Summary: Chlorosomes from green bacteria have efficient light capture and energy transfer abilities, inspiring developments in artificial light-harvesting and molecular optoelectronics. We examine two different organizations of the molecular building blocks and show how they affect light capture and energy transfer, potentially allowing the bacteria to adapt to changes in light conditions. Spectral simulations reveal how changes in the helicity of chlorosomal aggregates alter energy transfer, with contrasting energy pathways in different chlorosomes.
JOURNAL OF PHYSICAL CHEMISTRY B
(2023)
Article
Plant Sciences
Yuxi Niu, Dusan Lazar, Alfred R. Holzwarth, David M. Kramer, Shizue Matsubara, Fabio Fiorani, Hendrik Poorter, Silvia D. Schrey, Ladislav Nedbal
Summary: The study focused on the rapid regulation of photoprotective mechanisms in response to changing light in plants. It was found that PsbS regulation had the fastest response time to oscillating light with periods longer than 10 seconds. Processes involving violaxanthin de-epoxidase dampened changes in chlorophyll fluorescence in oscillation periods of 2 minutes or longer. Knocking out the PGR5/PGRL1 pathway strongly reduced variations in all monitored parameters, possibly due to congestion in the electron transport. The observed dynamics of photosystem I components were interpreted as being formed by thylakoid remodeling in slow light oscillations.
Review
Chemistry, Multidisciplinary
Gerd Buntkowsky, Franziska Theiss, Jonas Lins, Yuliya A. Miloslavina, Laura Wienands, Alexey Kiryutin, Alexandra Yurkovskaya
Summary: Nuclear Magnetic Resonance (NMR) spectroscopy and Magnetic Resonance Imaging (MRI) are essential analytical and diagnostic tools with a wide range of applications. However, the low sensitivity of magnetic resonance poses a major challenge in various fields. Parahydrogen induced polarization (PHIP) is a cost-effective method to enhance signal by utilizing chemical reactions. This article provides an overview of PHIP techniques and reviews current literature on method developments and applications.
Article
Biochemistry & Molecular Biology
Gabor Sipka, Melinda Magyar, Alberto Mezzetti, Parveen Akhtar, Qingjun Zhu, Yanan Xiao, Guangye Han, Stefano Santabarbara, Jian-Ren Shen, Petar H. Lambrev, Gyozo Garab
Summary: The study reveals that the light-adapted charge-separated state in PSII undergoes a transition from the closed state, contributing to variable chlorophyll-a fluorescence and involving complex photochemical and photophysical reactions. The highest fluorescence level is associated with the light-adapted state rather than the closed state, challenging the traditional understanding of fluorescence parameters in PSII quantum efficiency assessment.