Article
Integrative & Complementary Medicine
Taibah Aldakhil, Saud O. Alshammari, Bushra Siraj, Bishoy El-Aarag, Shamshad Zarina, David Salehi, Aftab Ahmed
Summary: This study characterized the nsLTP1 from caraway seeds and investigated its three-dimensional structure and protein-ligand interactions through docking studies. The study also evaluated the cytotoxic and antioxidant activities of nsLTP1 and demonstrated its thermal stability.
BMC COMPLEMENTARY MEDICINE AND THERAPIES
(2023)
Article
Biochemistry & Molecular Biology
Bao Yang, Maoyin Li, Anne Phillips, Long Li, Usman Ali, Qing Li, Shaoping Lu, Yueyun Hong, Xuemin Wang, Liang Guo
Summary: Phosphate is essential for plant growth, and NPC4 enzyme plays a positive role in root growth by hydrolyzing and remodeling sphingolipids during phosphate deficiency in Arabidopsis.
Article
Biochemistry & Molecular Biology
Zaid Kamal Madni, Amit Kumar, Ujjwal Kumar, Deepika Jaiswal, Dinakar M. Salunke
Summary: Nonspecific lipid transfer proteins are multifunctional seed proteins that can bind and transfer different lipid molecules. The crystal structure and molecular dynamics simulation of the lipid transfer protein from Solanum melongena reveal the mobility and orientation change of lipid molecules inside the hydrophobic cavity.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Review
Biochemistry & Molecular Biology
Yingying Song, Jing Gu, Jingya You, Yiyang Tao, Yao Zhang, Lizhuo Wang, Jialin Gao
Summary: Sidt2 is a lysosomal membrane protein that plays a crucial role in maintaining the normal morphology and stability of lysosomes, as well as regulating glucose and lipid metabolism and nucleotide transport.
Article
Plant Sciences
Khawla Missaoui, Zulema Gonzalez-Klein, Diego Pazos-Castro, Guadalupe Hernandez-Ramirez, Maria Garrido-Arandia, Faical Brini, Araceli Diaz-Perales, Jaime Tome-Amat
Summary: Plant non-specific lipid transfer proteins (nsLTPs) are small, basic proteins with a wide distribution in higher plants. They have a stable structure that allows them to bind and transport hydrophobic molecules. These proteins play critical roles in plant development and response to various stresses.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Food Science & Technology
Ankita Mishra, Ashok Kumar
Summary: Nonspecific lipid transfer proteins (nsLTPs) are panallergens found widely across different plants. This study used in-silico methods to identify B-cell epitopes for LTPs from various legumes, showing significant homology between chickpea and mung-bean LTPs and other allergenic LTPs. The findings suggest potential cross-reactivity among LTPs from different plant sources based on shared B-cell epitopes.
Article
Oncology
Chao Deng, Siming Gong, Lizhen Lin, Juyu Tang, Xiaoyang Pang, Panfeng Wu
Summary: Heat shock protein family A member 5 (HSPA5) is crucial in the endoplasmic reticulum stress and unfolded protein response (UPR) pathways, which play important roles in protein folding and quality control. HSPA5 is overexpressed in various types of tumors and is associated with poor prognosis. It is also correlated with immune checkpoints and alterations in the immune landscape. HSPA5 may serve as a potential target for cancer treatment.
AMERICAN JOURNAL OF CANCER RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Djamila Onesime, Lea Vidal, Stephane Thomas, Celine Henry, Veronique Martin, Gwenaelle Andre, Piotr Kubiak, Philippe Minard, Ewelina Celinska, Jean-Marc Nicaud
Summary: Yarrowia lipolytica has been found to have a family of extracellular fatty acid binding proteins, proposed to be named eFbp1 to eFbp4. The exact mechanism of action of these proteins remains to be elucidated, but they are expected to have applications in lipid biotechnology, such as improving fatty acid production and bioconversion.
MICROBIAL CELL FACTORIES
(2022)
Article
Pharmacology & Pharmacy
Romain Marteau, Severine Ravez, Darius Mazhari Dorooee, Hind Bouchaoui, Karine Porte, Jean-Christophe Devedjian, Patricia Melnyk, David Devos, Raphael Frederick, Jamal El Bakali
Summary: Ferroptosis is an iron-dependent regulated cell death characterized by the accumulation of lipid peroxides, and recent studies have shown that ACSL4 inhibitors are emerging as attractive agents for anti-ferroptosis. Screening FDA-approved drug library has identified new inhibitors with micromolar-range activities against ACSL4, with sertaconazole being the most potent inhibitor with an IC50 of 280 nM against hACSL4.
BIOCHEMICAL PHARMACOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Roman Holic, Dominik Stastny, Peter Griac
Summary: This paper provides an overview of Sec14 family of lipid transfer proteins (LTPs) in the well-studied yeast Saccharomyces cerevisiae and other selected non-Saccharomyces yeasts. It discusses the specificities, mode of action, subcellular localization, and physiological functions of Sec14-domain LTPs in various yeasts, as well as their significance in antifungal drug targeting and modifying drug resistance in pathogenic yeasts.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)
Review
Biochemistry & Molecular Biology
Simon Snoeck, Natalia Guayazan-Palacios, Adam D. Steinbrenner
Summary: This review discusses the mechanisms by which plants perceive and respond to chewing and piercing-sucking herbivores. It examines the recognition of herbivore-associated molecular patterns (HAMPs) and effectors by plant immune systems, and emphasizes the importance of these initial molecular interactions in resistance. The review also explores the potential evolution of immune receptor functions and the integration of sensing functions in plant-herbivore interactions.
Article
Biochemistry & Molecular Biology
Xiaodong Cui, Wenhua Tian, Wenhua Wang, Jiao Li, Zhuanhua Wang, Chen Li
Summary: This study investigates the biochemical characteristics and allergenicity of two non-specific lipid transfer proteins from Tartary buckwheat. It is found that one of the proteins (FtLTP1b) specifically binds IgE in the serum allergic to buckwheat and shows cross-reactivity with pollen, making it a novel allergenic member.
PROTEIN AND PEPTIDE LETTERS
(2023)
Article
Biotechnology & Applied Microbiology
Rui Zheng, Xiangbing Meng, Qingliang Hu, Bo Yang, Guicai Cui, Yingying Li, Siju Zhang, Yu Zhang, Xuan Ma, Xiaoguang Song, Shanshan Liang, Yunhai Li, Jiayang Li, Hong Yu, Weijiang Luan
Summary: OsFTL12 in rice functions as an antagonist to Hd3a and RFT1 by forming the florigen repression complex (FRC) with GF14b and OsFD1. OsFTL12 suppresses the expression of the floral identity genes OsMADS14 and OsMADS15, affecting rice panicle development and leaf growth. These findings reveal that different FT-like members can fine-tune heading date and plant architecture in rice by regulating the balance of FAC and FRC.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Review
Plant Sciences
Hang Gao, Kang Ma, Guojie Ji, Liying Pan, Qingfeng Zhou
Summary: Nonspecific lipid transfer proteins (LTPs) are small, cysteine-rich proteins that play important roles in plant growth and development, including inhibiting fungal or bacterial growth in plant immune processes. This review summarizes the roles of LTPs in plant-pathogen interactions, with an emphasis on the molecular mechanisms underlying plant immune responses and specific LTP functions.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Plant Sciences
Alberto Vangelisti, Samuel Simoni, Gabriele Usai, Flavia Mascagni, Maria Ventimiglia, Lucia Natali, Andrea Cavallini, Tommaso Giordani
Summary: This study identified and characterized the LTP multigenic family in sunflower using the reliable genome sequence, finding a total of 101 putative LTP genes. Sunflower LTPs were categorized into four clades, reflecting their genomic and structural organization. Further analysis showed the prevalence of tandem and whole genome duplication events in the origin of these genes, in line with the polyploidization events in sunflower genome evolution. The expression of LTP genes in response to external stimuli, particularly abscisic acid, auxin, and saline environment, was demonstrated, with some genes exclusively expressed in sunflower seeds.
Article
Food Science & Technology
Rong Wang, Haruyo Nakajima-Adachi, Yimei Wang, Yingyu Zhou, Wenting Gu, Erika Hiraide, Mamiko Morinaga, Ryogo Nakagawa, Shotaro Nakamura, Tomohiro Takano, Xuyang Li, Mayumi Saeki, Osamu Kaminuma, Takachika Hiroi, Kenji Uchida, Hidemasa Motoshima, Masaru Tanokura, Takuya Miyakawa, Satoshi Hachimura
Summary: Lactococcus lactis subsp. cremoris YRC3780 isolated from kefir has anti-allergic effects in humans. The oral administration of L. cremoris YRC3780 enhanced IFN-gamma and IL-12 production, inhibited IL-4 production, and attenuated symptoms in an atopic dermatitis mouse model by regulating cytokine expression.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Multidisciplinary Sciences
Takuya Miyakawa, Jian Yang, Masato Kawasaki, Naruhiko Adachi, Ayumu Fujii, Yumiko Miyauchi, Tomonari Muramatsu, Toshio Moriya, Toshiya Senda, Masaru Tanokura
Summary: This study solves the cryo-EM structures of CphA1 and reveals the aspartate binding mode and protein dynamics required for cyanophycin elongation. The research also suggests a potential role of protein dynamics in the catalytic efficiency of the arginine condensation reaction.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Chao Li, Xin Gao, Hongbin Qi, Wei Zhang, Lei Li, Cancan Wei, Meijing Wei, Xiaoxuan Sun, Shusen Wang, Liyan Wang, Yingbin Ji, Shuhong Mao, Zhangliang Zhu, Masaru Tanokura, Fuping Lu, Hui-Min Qin
Summary: In this study, a genetically encoded biosensor based on a D-allulose-responsive transcriptional regulator was redesigned for real-time monitoring of D-allulose. Furthermore, an ultrahigh-throughput droplet-based microfluidic screening platform was constructed for the directed evolution of ketose 3-epimerases (KEases). Structural analysis revealed a lid conformation in Sinorhizobium fredii D-allulose 3-epimerase (SfDAE) that regulates substrate recognition, and through rational design and directed evolution, a mutant M3-2 with 17-fold enhanced catalytic efficiency was identified. This research provides a paradigm for the design and optimization of biosensor-based microdroplet screening platforms.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Multidisciplinary Sciences
Satoshi Kidokoro, Izumi Konoura, Fumiyuki Soma, Kazuo Shinozaki, Takamasa Suzuki, Masaru Tanokura, Takuya Miyakawa, Takato Imaizumi, Kazuko Yamaguchi-Shinozaki
Summary: Plants respond to severe temperature changes by inducing the expression of numerous genes whose products enhance stress tolerance and responses. DREB1 genes are rapidly and strongly induced by cold stress and their molecular mechanisms are vital for recognizing the initial responses to cold stress in plants.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Immunology
Yimei Wang, Tomohiro Takano, Yingyu Zhou, Rong Wang, Takayuki Toshimitsu, Toshihiro Sashihara, Masaru Tanokura, Takuya Miyakawa, Haruyo Nakajima-Adachi, Satoshi Hachimura
Summary: This study investigated the effects of Lactiplantibacillus plantarum OLL2712 on obesity-induced inflammation. It was found that the LAB strain regulated gut microbiota, suppressed intestinal inflammation, and improved gut barrier function, thereby reducing obesity-related inflammation.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biology
Akira Nozawa, Ryoko Miyazaki, Yoshinao Aoki, Reina Hirose, Ryosuke Hori, Chihiro Muramatsu, Yukinori Shigematsu, Keiichirou Nemoto, Yoshinori Hasegawa, Keiko Fujita, Takuya Miyakawa, Masaru Tanokura, Shunji Suzuki, Tatsuya Sawasaki
Summary: A new gibberellin receptor agonist, diphegaractin, has been identified in this study, which shows gibberellin-like activity in the growth and maturation of grapes, lettuce, Arabidopsis, and citrus fruit. This is the first successful identification of a GA receptor agonist with GA-like activity in agricultural plants using an in vitro molecular-targeted drug screening system.
COMMUNICATIONS BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Satoshi Yamanaka, Hirotake Furihata, Yuta Yanagihara, Akihito Taya, Takato Nagasaka, Mai Usui, Koya Nagaoka, Yuki Shoya, Kohei Nishino, Shuhei Yoshida, Hidetaka Kosako, Masaru Tanokura, Takuya Miyakawa, Yuuki Imai, Norio Shibata, Tatsuya Sawasaki
Summary: Lenalidomide is an effective drug for treating hematological cancers, but has teratogenic effects on fetuses. This study identifies modifications that enhance the selectivity and effectiveness of lenalidomide as a standalone molecular glue or integrated in PROTACs.
NATURE COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Hiroyuki Takeda, Tatsuhiko Ozawa, Hiroki Zenke, Yoh Ohnuki, Yuri Umeda, Wei Zhou, Honoka Tomoda, Akihiko Takechi, Kimiyoshi Narita, Takaaki Shimizu, Takuya Miyakawa, Yuji Ito, Tatsuya Sawasaki
Summary: This study investigated the use of the Japanese topeshark as an immune animal for the development of VNAR antibodies. By injecting Venus fluorescent protein into the sharks, the levels of antigen-specific IgM and IgNAR antibodies in the blood were significantly increased. Phage-display and yeast-display libraries were constructed using RNA from immunized shark splenocytes, and multiple antigen-specific VNAR antibodies were obtained. These antibodies exhibited high affinity and plasticity, and retained their binding activity even after high-temperature or reducing-agent treatment. Deep sequencing analysis revealed distinct enrichment bias in the CDR3-coding sequences between libraries. Overall, the Japanese topeshark shows promise for the development of VNAR antibodies.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Takafumi Hashimoto, Kenji Hashimoto, Hiroki Shindo, Shoko Tsuboyama, Takuya Miyakawa, Masaru Tanokura, Kazuyuki Kuchitsu
Summary: This study reveals a mechanism of Ca2+ and phosphorylation regulating the activity of MpRBOHB, highlighting the importance of Ca2+ binding in this process. This mechanism is likely to be conserved among land plant RBOHs.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Food Science & Technology
Wanyi Chiu, Tomonari Muramatsu, Yingyu Zhou, Takuya Miyakawa, Masaru Tanokura
Summary: This study investigated the changes in compounds in Virginia-type peanut seeds and peels during roasting. The results showed an increase in taste and aroma compounds, as well as functional benefits of roasted peanuts.
ACS FOOD SCIENCE & TECHNOLOGY
(2022)
Article
Plant Sciences
Shohei Nosaki, Nobutaka Mitsuda, Shingo Sakamoto, Kazuki Kusubayashi, Ayumi Yamagami, Yuqun Xu, Thi Bao Chau Bui, Tohru Terada, Kenji Miura, Takeshi Nakano, Masaru Tanokura, Takuya Miyakawa
Summary: The transcription factor BIL1/BZR1 is involved in regulating gene expression in response to brassinosteroid hormones in plants. This study reveals that BIL1/BZR1 can repress the transcription of certain genes by binding tightly to specific regions on the DNA. Furthermore, the study demonstrates that BIL1/BZR1 achieves selectivity for particular DNA sequences by reading the shape of the DNA rather than directly recognizing the nucleobases. These findings provide valuable insights into the molecular and structural basis of transcriptional repression by BIL1/BZR1 and its role in gene regulation.