Article
Biochemistry & Molecular Biology
Prashant R. R. Singh, Jainendra Pathak, Rajneesh, Haseen Ahmed, Donat -P. P. Haeder, Rajeshwar P. Sinha
Summary: Cyanobacteria use circadian rhythms to cope with daily light fluctuations and the dark phase plays a significant role in protecting them from the negative impact of continuous light exposure. This study provides insights into the physiological responses of cyanobacteria to changing light environments.
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
(2023)
Article
Engineering, Environmental
Javier Garcia-Calleja, Thibaut Cossart, Zoyne Pedrero, Joao P. Santos, Laurent Ouerdane, Emmanuel Tessier, Vera Slaveykova, David Amouroux
Summary: Understanding the complexation of mercury (Hg) with low molecular weight (LMW) bioligands can help elucidate its speciation, and the use of isotopically labeled Hg species in cyanobacteria has provided insights into the role of intracellular biogenic ligands in the speciation of iHg and MeHg.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Biochemical Research Methods
Maurice Mager, Hugo Pineda Hernandez, Fabian Brandenburg, Luis Lopez-Maury, Alistair J. McCormick, Dennis J. Nuernberg, Tim Orthwein, David A. Russo, Angelo Joshua Victoria, Xiaoran Wang, Julie A. Z. Zedler, Filipe Branco dos Santos, Nicolas M. Schmelling
Summary: In recent years, many new synthetic biology tools have been developed for use in cyanobacteria. However, the reported characterizations of these tools often cannot be reproduced, which greatly limits comparability of results and applicability. This study assessed the reproducibility of a standard microbiological experiment in Synechocystis sp. PCC 6803 and found significant differences in spectrophotometer measurements and growth rates between laboratories. These findings highlight the need for additional reporting requirements of growth conditions for phototrophic organisms beyond light intensity and CO2 supply.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Mathematical & Computational Biology
Amit Kugler, Karin Stensjo
Summary: Understanding energy and redox homeostasis, as well as carbon partitioning, is crucial for systems metabolic engineering of cell factories. Stoichiometric metabolic modeling and network-wide analysis revealed that the rate of NAD(P)H regeneration controls the ATP/NADPH ratio, and energy and redox balance is interconnected with carbon and nitrogen metabolism. An auxiliary pathway was also identified to support cellular redox homeostasis and ATP cycling.
NPJ SYSTEMS BIOLOGY AND APPLICATIONS
(2023)
Article
Plant Sciences
Yumeng Zhang, Yaqi Wang, Wei Wei, Min Wang, Shuzhao Jia, Mingkun Yang, Feng Ge
Summary: Protein homeostasis in cyanobacteria is regulated by a proteolytic complex called ClpXP, which consists of ATPase ClpX and peptidase ClpP. This study aimed to investigate the functions and regulatory networks of ClpX in Synechocystis by identifying 172 differentially expressed proteins upon clpX interruption. The results provide a comprehensive ClpX-regulated protein network and new candidates for future functional studies on ClpX in cyanobacteria.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Christopher J. Gisriel, Jimin Wang, Jinchan Liu, David A. Flesher, Krystle M. Reiss, Hao-Li Huang, Ke R. Yang, William H. Armstrong, M. R. Gunner, Victor S. Batista, Richard J. Debus, Gary W. Brudvig
Summary: The cryo-electron microscopy structure of PSII from Synechocystis sp. PCC 6803 has been determined at high resolution, revealing differences from thermophilic cyanobacterial PSII structures that significantly impact the understanding of PSII structure and the mechanism of water oxidation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biology
Kaisei Maeda, Yukiko Okuda, Gen Enomoto, Satoru Watanabe, Masahiko Ikeuchi
Summary: Extracellular polysaccharides of bacteria play crucial roles in biofilm formation, stress tolerance, and infectivity, with cyanobacteria uniquely producing sulfated polysaccharides to support phototrophic biofilms. Little is known about the biosynthesis and function of these polysaccharides in cyanobacteria, but a study on Synechocystis sp. strain PCC 6803 has identified the genes responsible for the synthesis of a sulfated polysaccharide called synechan, potentially leading to insights into the functions and biotechnological applications of similar polysaccharides found in cyanobacteria genomes.
Article
Biotechnology & Applied Microbiology
Kateryna Kukil, Elias Englund, Nick Crang, Elton P. Hudson, Pia Lindberg
Summary: In this study, phenylalanine resistant mutant strains of the unicellular cyanobacterium Synechocystis sp. PCC 6803 were generated by laboratory evolution. The mutant strains were able to secrete phenylalanine and showed potential for production of trans-cinnamic acid and para-coumaric acid. The PRM8 strain demonstrated the highest specific production of these compounds.
METABOLIC ENGINEERING
(2023)
Article
Microbiology
Dorota Muth-Pawlak, Sanna Kreula, Peter J. Gollan, Tuomas Huokko, Yagut Allahverdiyeva, Eva-Mari Aro
Summary: This study analyzed the proteomes of photosynthetic cyanobacteria under different conditions and found differential expression of proteins related to photosynthesis and carbon metabolism. The study also discovered unique expression patterns under specific conditions and concluded that combining photosynthetic activity with high intracellular inorganic carbon conditions promotes excellent growth in the bacteria.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Henna Mustila, Dorota Muth-Pawlak, Eva-Mari Aro, Yagut Allahverdiyeva
Summary: In this study, the global proteome of Synechocystis sp. PCC 6803 under fluctuating light conditions was compared with constant light conditions. It was found that cell metabolism was clearly reprogrammed, and there were correlations between mRNA and protein levels to some extent. The data suggest that higher nitrogen assimilation is maintained for long-term acclimation to fluctuating light upon CO2 step-down in Synechocystis sp. PCC 6803.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Biology
Otso Turunen, Satu Koskinen, Juha Kurkela, Outi Karhuvaara, Kaisa Hakkila, Taina Tyystjaervi
Summary: Acclimation of cyanobacterium Synechocystis sp. PCC6803 to suboptimal conditions is largely dependent on adjustments of gene expression, which is highly controlled by the sigma factor subunits of RNA polymerase. The study shows that the SigB and SigD sigma factors are both induced in high light and heat stresses, regulating different sets of genes depending on the stress conditions.Furthermore, favoring either SigB or SigD factor by deleting other group 2 sigma factors does not lead to superior acclimation to high light or high temperature, indicating the importance of all group 2 sigma factors in acclimation processes.
Article
Biochemistry & Molecular Biology
Shiqi Lin, Shiliang Li, Tong Ouyang, Gu Chen
Summary: Excess ammonium causes toxicity and stress in cyanobacteria, and how cyanobacteria respond to ammonium stress is not well understood. In this study, a gene called Slr1821 was found to be a crucial regulator for ammonium stress response in Synechocystis sp. PCC6803. The expression of many genes involved in nitrogen uptake, assimilation, and carbon regulation depended on the presence of Slr1821, highlighting its importance in maintaining carbon/nitrogen balance under high nitrogen conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Takashi Inada, Shuntaro Machida, Koichiro Awai, Iwane Suzuki
Summary: Microalgal lipids and fatty acids are important components for biofuel production due to their high productivity potential. Genetic modification can lead to the synthesis of (omega-1)-hydroxy fatty acids in cyanobacteria, but high light conditions may inhibit photosynthesis and growth of the strain.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Plant Sciences
Sandeesha Kodru, Laszlo Sass, Priyanka Patil, Milan Szabo, Imre Vass
Summary: In this study, a TL component similar to the plant AG band, originating from NDH-1-mediated CEF, was identified in cyanobacteria. The +40 degrees C band was most efficiently induced by FR illumination at -10 degrees C, and it was observed that low temperatures block CEF at two different sites in Synechocystis PCC 6803.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Genetics & Heredity
Chen Xu, Bing Wang, Lin Yang, Lucas Zhongming Hu, Lanxing Yi, Yaxuan Wang, Shenglan Chen, Andrew Emili, Cuihong Wan
Summary: In this study, using co-fractionation coupled with LC-MS/MS, the researchers identified 291 multi-protein complexes and 24,092 protein-protein interactions in Synechocystis sp. PCC 6803. These interactions reveal the roles of photosynthesis in various physiological processes and highlight the differences in protein functions between bacteria and higher plants.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2022)
Article
Biology
Olli Virtanen, Emanuella Constantinidou, Esa Tyystjarvi
Summary: Plant leaves are green because chlorophyll absorbs blue and red light, rather than reflecting green light. Leaves deficient in chlorophyll reflect green light more efficiently than green leaves. Cellulose within plant leaves is the main component responsible for diffuse reflection of visible light.
JOURNAL OF BIOLOGICAL EDUCATION
(2022)
Article
Plant Sciences
H. Mattila, D. Valev, K. B. Mishra, V. Havurinne, O. Virtanen, M. Antinluoma, E. Tyystjarvi
Summary: The growth rates during batch and turbidostat modes varied independently of each other, indicating that a growth rate measured in a batch cannot be used to determine the continuous growth rate. Different photoinhibition susceptibilities in tested algae suggest different amounts of energy invested in repair. However, photoinhibition tolerance did not necessarily lead to a fast growth rate at a moderate light intensity.
Article
Multidisciplinary Sciences
Risto Kalliola, Ari Linna, Kalle Ruokolainen, Esa Tyystjarvi, Carl Lange
Summary: In this study, we investigated the element distribution in Guadua bamboo leaves using scanning electron microscopy with energy-dispersive X-ray spectroscopy. The results showed high silicon content and low calcium and potassium content in the leaves. Silicon was mainly found in bulliform cells, bilobate shaped short cells, and stomata. Potassium often surrounded silicon-loaded cells, and silicon and potassium had overlapping distributions in the intercostal areas near vein margins. Calcium showed abundant spotted distribution in the intercostal areas. Moreover, adjacent fusoid cells showed different combinations of silicon, potassium, and calcium, suggesting potentially variable functions of these cells.
SN APPLIED SCIENCES
(2022)
Article
Cell Biology
Kalle Sipila, Emanuel Rognoni, Johanna Jokinen, Mukul Tewary, Matteo Vietri Rudan, Salli Talvi, Ville Jokinen, Kathe M. Dahlstrom, Kif Liakath-Ali, Atefeh Mobasseri, Xinyi Du-Harpur, Jarmo Kapyla, Stephen L. Nutt, Tiina A. Salminen, Jyrki Heino, Fiona M. Watt
Summary: In this study, the unique expression of the transmembrane protein embigin in the sebaceous gland was investigated. It was found that embigin negatively regulates the number of cells expressing it through Wnt signaling. Additionally, embigin was shown to affect lipid metabolism and sebaceous niche architecture by influencing extracellular matrix organization and basolateral targeting of monocarboxylate transport. It was also discovered that embigin acts as a direct fibronectin receptor, without impairing integrin function.
DEVELOPMENTAL CELL
(2022)
Article
Pharmacology & Pharmacy
Thomas Boehm, Marion Alix, Karin Petroczi, Serhii Vakal, Elisabeth Gludovacz, Nicole Borth, Tiina A. Salminen, Bernd Jilma
Summary: Nafamostat is a potent human DAO inhibitor and may increase the severity of anaphylactic reactions by interfering with DAO-mediated extracellular histamine degradation.
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2022)
Article
Virology
Shreya Saha, Andres Lohmus, Pinky Dutta, Maija Pollari, Kristiina Makinen
Summary: Potyviral CP and HCPro play key roles in viral gene expression regulation and particle formation. HCPro relieves CP-mediated inhibition of PVA RNA expression and enables CPs to be sequestered to particles. Formation of PVA particles requires replication, and a large ribonucleoprotein complex formed around VPg at one end of the particles is essential for their integrity.
Article
Plant Sciences
Heta Mattila, Sujata Mishra, Taina Tyystjarvi, Esa Tyystjarvi
Summary: Singlet oxygen (O-1(2)) has both harmful and signaling functions in photosynthesis. This study investigated the temperature dependence of O-1(2) production, photoinhibition, and recombination pathways. The results suggest that the miss-associated recombination of P(680)(+)Q(A)(-) is the main source of O-1(2). Furthermore, three parallel photoinhibition mechanisms were identified, with the manganese mechanism dominant in UV radiation and light absorption by Chls mechanism dominating in red light.
Article
Plant Sciences
Olli Virtanen, Esa Tyystjarvi
Summary: This study used HPLC to determine the redox state of the plastoquinone pool (PQ-pool) in Chlamydomonas reinhardtii and compared it with the light state. The results showed that the dynamics of the PQ-pool in C. reinhardtii under different light conditions are more complicated than in plants, possibly due to the larger number of LHC units and less different absorption profiles of the photosystems in C. reinhardtii.
PHOTOSYNTHESIS RESEARCH
(2023)
Article
Plant Sciences
Heta Mattila, Esa Tyystjarvi
Summary: Photosynthetic organisms, such as evergreen plants, may be damaged by strong light at low temperatures. This light-induced damage depends on singlet oxygen and is less pronounced in winter leaves of evergreen plants compared to thylakoids of summer leaves. However, cyanobacteria are not as protected from photoinhibition as evergreen plants, despite their high carotenoid levels.
PHYSIOLOGIA PLANTARUM
(2022)
Article
Plant Sciences
Taras K. Antal, Alena A. Volgusheva, Galina P. Kukarskikh, Evgeniy P. Lukashev, Alexander A. Bulychev, Andrea Margonelli, Silvia Orlanducci, Gabriella Leo, Luciana Cerri, Esa Tyystjarvi, Maya D. Lambreva
Summary: Research has found that single-walled carbon nanotubes (SWCNTs) can protect algal photosynthesis against photoinhibition, and intentional selection of nanomaterial characteristics can overcome their inherent phytotoxicity.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Virology
Maija Pollari, Nina Sipari, Sylvain Poque, Kristiina Himanen, Kristiina Makinen
Summary: Mixed virus infections can lead to viral synergism and threaten crop production. In co-infection with potato virus A (PVA) and potato virus X (PVX), severe symptoms and impaired growth were observed in Nicotiana benthamiana. The metabolic response in co-infected plants differed significantly from singly infected ones, with an increase in defense-related metabolites and stress indicators. The imbalance in methylation potential and oxidative stress indicate that the activated defense pathways are insufficient to counter the impact of the co-infection on plant growth.
Article
Biology
Chanchanok Duangsri, Tiina A. Salminen, Marion Alix, Sarawan Kaewmongkol, Nattaphong Akrimajirachoote, Wanthanee Khetkorn, Sathaporn Jittapalapong, Pirkko Maenpaa, Aran Incharoensakdi, Wuttinun Raksajit
Summary: Polyhydroxybutyrate (PHB) is a biocompatible and biodegradable polymer with potential applications as a replacement for fossil-derived polymers. The key enzyme for PHB production in Arthrospira platensis is PHA synthase (PhaC). This study constructed recombinant E. cloni ((R))10G cells carrying the A. platensis phaC gene (rPhaC(Ap)) and characterized the catalytic properties and structure of the overexpressed rPhaC(Ap). The obtained model of PhaC(Ap) revealed its asymmetric dimeric structure and the involvement of specific residues in substrate binding and dimerization.
Article
Cardiac & Cardiovascular Systems
Arghavan Jahandideh, Jenni Virta, Xiang-Guo Li, Heidi Liljenback, Olli Moisio, Jesse Ponkamo, Noora Rajala, Marion Alix, Jukka Lehtonen, Mikko I. Mayranpaa, Tiina A. Salminen, Juhani Knuuti, Sirpa Jalkanen, Antti Saraste, Anne Roivainen
Summary: This study successfully demonstrated the feasibility of using [Ga-68]Ga-DOTA-Siglec-9 PET imaging to detect myocardial lesions in rats with autoimmune myocarditis, suggesting its potential as a novel technique.
JOURNAL OF NUCLEAR CARDIOLOGY
(2023)
Article
Plant Sciences
Marjaana Rantala, Paula Mulo, Esa Tyystjaervi, Heta Mattila
Summary: During autumn senescence, the disassembly and degradation of photosynthetic protein complexes is poorly understood, as well as the reason why leaves accumulate red pigments. One possibility is that the red pigments, or anthocyanins, protect the senescing leaves from excess light.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Forestry
Heta Mattila, Esa Tyystjarvi
Summary: The reasons behind autumn colors, a striking manifestation of anthocyanin synthesis in plants, are poorly understood. In this study, the researchers investigated the role of red pigments in senescing leaves, and found that they do not provide photoprotection. Instead, the primary function of anthocyanin synthesis appears to be to dispose of carbohydrates, allowing the light reactions to produce energy for nutrient translocation during the last phase of autumn senescence.