Article
Biochemistry & Molecular Biology
Shengyuan Guo, Chuanjiang Zheng, Yan Wang, Yangwen Xu, Jinwen Wu, Lan Wang, Xiangdong Liu, Zhixiong Chen
Summary: In this study, the regulatory role of a rice-specific miRNA, OsmiR5488, was characterized. The overexpression of OsmiR5488 led to a reduction in seed-setting rate and abnormal embryo sacs in rice. The target gene of OsmiR5488 was identified as OsARF25. Additionally, the overexpression of miR5488 resulted in a decrease in primary root length with increasing auxin concentrations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Lenka Luptakova, Simona Dvorcakova, Zuzana Demcisakova, Lassaad Belbahri, Katarina Holovska, Eva Petrovova
Summary: The study revealed that DMSO has embryotoxic effects on developing chicken embryos, sometimes resulting in malformations. At the molecular level, DMSO affects the expression of CYP genes. Additionally, increasing doses of DMSO lead to higher embryo mortality rates.
Review
Toxicology
Ann-Kathrin Loerracher, Thomas Braunbeck
Summary: There is a strong trend towards using zebrafish embryos as translational models in ecotoxicological and toxicological testing strategies, leading to an increasing need to understand their biotransformation capacity. However, there is still insufficient research on the expression patterns and activities of CYP isoforms in juvenile and adult zebrafish. Studies have shown qualitative similarities but quantitative differences in metabolic activities between zebrafish embryos and later developmental stages, highlighting the importance of further research to assess metabolic profiles and capacities of CYP isoforms in zebrafish.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Multidisciplinary Sciences
Laurentijn Tilleman, Kaat Rubben, Wim Van Criekinge, Dieter Deforce, Filip Van Nieuwerburgh
Summary: This study tested the effectiveness of Targeted Locus Amplification (TLA) combined with Illumina or Nanopore sequencing in accurate genotyping and phasing of pharmacogenes. The results showed limited nucleotide mapping on target and incomplete coverage of genes with both sequencing methods. However, the optimized primer pair for the CYP2D6 gene in the Nanopore dataset resulted in correct haplotypes, indicating the potential reliability of this method with patient-specific primer design and optimization.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Jing Liu, Lauritz Harken, Yiling Yang, Xiulan Xie, Shu-Ming Li
Summary: This study reports two important gene clusters involved in the biosynthesis of mycocyclosin and guatyromycine. By reconstructing the biosynthetic pathways and conducting biochemical investigations, it was found that the enzymes encoded by these genes can catalyze both intramolecular oxidation and intermolecular nucleobase transfer reactions, leading to the synthesis of specific natural products.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemistry & Molecular Biology
Tianyi Yu, Marzhana Omarova, Meng Zhang, Istiak Hossain, Jianqiang Chen, Omead Darvish, Vijay T. John, Donghui Zhang
Summary: The cellular functions of membrane proteins are closely related to their structures and aggregation states. We have investigated the fragmentation of synthetic liposomes using hydrophobe-containing polypeptoids (HCPs). Our findings demonstrate that HCPs can effectively induce the fragmentation of liposomes into colloidally stable nanoscale HCP-lipid complexes, and have the potential for novel macromolecular surfactants for membrane protein extraction.
Article
Biochemistry & Molecular Biology
Freeborn Rwere, Sangchoul Im, Lucy Waskell
Summary: By altering the 140s FMN binding loop of CYPOR, the activity with cyt P450s can be affected, with the mutant activity primarily determined by its ability to reduce ferric cyt P450. The different behaviors of the mutants are mainly due to the altered structure and mobility of the loop.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Lulu Ni, Ping Miao, Jian Jiang, Fang Wan, Jiangan Li, Min Ai, Lingzhong Kong, Su Tu
Summary: This study found that Glycyrrhiza promotes the metabolism of toxic components of Aconitum by upregulating the expression of CYP3A, reducing the content of heart failure-related markers, and improving the myocardial energy metabolism disorder, thus alleviating the development of congestive heart failure.
Article
Chemistry, Multidisciplinary
Xiaomin Qian, Isabella Nymann Westensee, Catarina Cavalheiro Fernandes, Brigitte Stadler
Summary: This study demonstrates the use of artificial enzymes to facilitate signal transfer between artificial cells and mammalian cells, opening up new opportunities for integrating the synthetic and living worlds with a bottom-up strategy.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Plant Sciences
Yupeng Hao, Zeyu Dong, Yongyan Zhao, Wenchen Tang, Xueqiang Wang, Jun Li, Luyao Wang, Yan Hu, Lei Fang, Xueying Guan, Fenglin Gu, Ziji Liu, Zhiyuan Zhang
Summary: This study identified and characterized P450 genes in pepper, showing that the P450 gene family has undergone massive duplication events and purification selection. Additionally, certain P450 genes were found to play important roles in placenta and pericarp development, while others were related to defense and phytohormone response in pepper.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Alecia R. Gee, Isobella S. J. Stone, Tegan P. Stockdale, Tara L. Pukala, James J. De Voss, Stephen G. Bell
Summary: We have achieved efficient oxidation reactions on unactivated carbon-hydrogen bonds by modifying the enzyme structure.
CHEMICAL COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Chao Li, Xue-Jiao Liu, Yan Yan, Mohammad Shah Alam, Zhen Liu, Zhen-Kun Yang, Ruo-Fu Tao, Er-kui Yue, Ming-Hua Duan, Jian-Hong Xu
Summary: Flowering time is crucial for rice reproduction and adaptation, controlled by the circadian clock. Mutations in OsLHY gene cause late flowering only under natural light conditions, affecting circadian clock-related genes expression and flowering regulators. OsLHY promotes rice flowering mainly by regulating Hd1 and Ehd1.
Article
Chemistry, Multidisciplinary
Thien-Kim Le, Jinhyun Kim, Ngoc Anh Nguyen, Thi Huong Ha Nguyen, Eun-Gene Sun, Su-Min Yee, Hyung-Sik Kang, Soo-Jin Yeom, Chan Beum Park, Chul-Ho Yun
Summary: Photobiocatalysis is a green platform for driving redox enzymatic reactions using solar energy without high-cost cofactors. Natural flavins were used as photosensitizers to develop a visible light-driven whole-cell platform for human cytochrome P450 (CYP) photobiocatalysis. The research showed that flavins can mediate light-driven biocatalysis by human CYPs in whole-cell systems, demonstrating the general applicability of solar-powered P450 photobiocatalytic system.
Article
Genetics & Heredity
Jie Chen, Xin Hao, Ruina Tan, Yang Li, Bowen Wang, Jialiang Pan, Wei Ma, Ling Ma
Summary: This study investigates the molecular characteristics and biological functions of Bx-cyp29A3 in B. xylophilus, providing insights into the immune defense against L(-)-carvone and establishing a basis for the study of new molecular targets for the prevention and management of B. xylophilus.
Article
Biochemistry & Molecular Biology
Jing Shen, Qian Yang, Lubo Hao, Lingling Zhang, Xuefeng Li, Mingqi Zheng
Summary: The newly discovered gene CYP77B34 was found to mediate Descurainia sophia L. resistance to tribenuron-methyl, and may have contributed to cross-resistance to other herbicides.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)