期刊
PLANT MOLECULAR BIOLOGY REPORTER
卷 33, 期 4, 页码 1107-1115出版社
SPRINGER
DOI: 10.1007/s11105-014-0805-2
关键词
Chloroplast transformation; Cu/Zn SOD; Recombinant proteins; Transplastomic plants; Tobacco
资金
- CSIR, India [CMM004]
- Department of Science Technology
- CSIR
An inexpensive source of industrially useful enzymes is critical for their commercial production. We have produced an industrially valuable recombinant superoxide dismutase (SOD) in tobacco chloroplasts. A gene from Withania somnifera, encoding a highly stable Cu/Zn SOD, was cloned into a chloroplast transformation vector. It expressed the SOD in tobacco chloroplasts following transformation. The transplastomic plants accumulated the recombinant SOD at up to similar to 9% of the total soluble protein in leaves. The purified chloroplast-expressed recombinant SOD had an estimated specific activity of similar to 4600 U/mg. Like the native enzyme, purified recombinant enzyme, prepared from tobacco leaves, was highly stable at high temperatures and tolerated a wide pH range, SDS, ethanol and protease treatment. The results establish the potential of chloroplast transformation for commercial production of recombinant SOD in plants.
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