Article
Biochemistry & Molecular Biology
Manish Sharma, Himashree Choudhury, Rajarshi Roy, Samantha A. Michaels, Kayode K. Ojo, Abhisheka Bansal
Summary: Calcium ions serve as important signals in various physiological processes. In malaria parasites, calcium dependent protein kinases (CDPKs) play critical roles in sensing and transducing calcium signals, establishing crosstalk with signaling pathways mediated by secondary messengers. CDPKs are essential for parasite development and transmission, making them valuable targets for anti-malarial drug development due to their absence in humans.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Multidisciplinary Sciences
Melissa Bredow, Kyle W. Bender, Alexandra Johnson Dingee, Danalyn R. Holmes, Alysha Thomson, Danielle Ciren, Cailun A. S. Tanney, Katherine E. Dunning, Marco Trujillo, Steven C. Huber, Jacqueline Monaghan
Summary: Phosphorylation of Ser318 is crucial for the activation of CPK28 in immune homeostasis and stem elongation, providing protection against immune response initiation in the absence of infection. In contrast, Ser318 phosphorylation is not necessary for stem elongation, indicating pathway-specific requirements for phosphorylation-based Ca2+-sensitivity priming. This research also suggests a potential biotechnological application in enhancing resistance to microbial pathogens without affecting yield through conserved Ser318 phosphorylation in related group IV CDPKs.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Shiqi Zhang, Ruixue Yu, Dongxue Yu, Pengjie Chang, Shiqi Guo, Xiaona Yang, Xinchun Liu, Chongyi Xu, Yuxin Hu
Summary: This study reveals the interplay between the calcium signaling module and auxin signaling in Arabidopsis, showing their important roles in callus and lateral root formation. The physical interaction between CaM-IQM and IAA proteins in a calcium-dependent manner is found to regulate auxin-induced callus formation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Plant Sciences
Eveline Y. Y. Kong, Julianne Biddle, Sundaravelpandian Kalaipandian, Steve W. W. Adkins
Summary: This study attempted to obtain friable coconut embryogenic callus (EC) through various approaches, but none of them were successful. However, some of the culture conditions were found to influence the formation of compact EC, which is important for further studies on somatic embryogenesis in coconut and other species.
Article
Cell Biology
Shikai Fan, Sha Yang, Guowei Li, Shubo Wan
Summary: This study identified 45 CDPK genes in cultivated peanut and investigated their roles in plant development and stress responses. The analysis revealed the genetic structure of these genes and their interaction with other proteins, as well as their expression patterns under different stress conditions.
Article
Agronomy
Hossein Ahmadi, Alireza Abbasi, Alireza Taleei, Valiollah Mohammadi, Jose J. Pueyo
Summary: In understanding the drought tolerance of canola, it is important to consider the efficiency of the antioxidant response and the potential involvement of calcium-dependent protein kinases (CDPKs). This study found that higher activities of antioxidant enzymes were observed in drought-tolerant canola cultivars, suggesting the importance of antioxidant response in drought tolerance. Additionally, the expression of BnaCDPK genes and the presence of stress-related elements in their promoter regions implied that CDPKs may play a role in regulating canola's response to drought stress.
Article
Plant Sciences
Consuelo Margarita Avila-Victor, Enrique de Jesus Arjona-Suarez, Leobardo Iracheta-Donjuan, Jorge Manuel Valdez-Carrasco, Fernando Carlos Gomez-Merino, Alejandrina Robledo-Paz
Summary: This study aimed to determine the influence of callus type, growth regulators, and phytagel concentration on the embryogenic capacity of the Colombia variety. The results showed that the compact callus type produced a greater number of embryos, and higher concentrations of phytagel resulted in larger quantities of differentiated embryos.
Article
Plant Sciences
Chitralekha Shyam, Manoj Kumar Tripathi, Sushma Tiwari, Niraj Tripathi, Ravindra Singh Solanki, Swapnil Sapre, Ashok Ahuja, Sharad Tiwari
Summary: Brassica juncea is a principal oilseed crop of India and Madhya Pradesh, with somaclonal variations explored as a potential source of additional variability for manipulating fatty acids, especially low erucic acid content. Putative somaclones derived from tissue cultures showed varying levels of erucic acid compared to parental lines, providing a potential source of variation for developing future mustard crops with low erucic acid content.
Review
Plant Sciences
Luis Alberto Bravo-Vazquez, Paola Isabel Angulo-Bejarano, Anindya Bandyopadhyay, Ashutosh Sharma, Sujay Paul
Summary: Plant regulatory noncoding RNAs (ncRNAs) play a key role in modulating gene expression during callus induction, and further research may lead to innovative plant tissue culture protocols.
PLANT CELL REPORTS
(2023)
Article
Plant Sciences
Elizabeth Alejandra Motolinia-Alcantara, Adrian Marcelo Franco-Vasquez, Antonio Nieto-Camacho, Roberto Arreguin-Espinosa, Mario Rodriguez-Monroy, Francisco Cruz-Sosa, Angelica Roman-Guerrero
Summary: It was found that in vitro culture of Ageratina pichichensis can produce phenolic compounds with antioxidant activity. Callus culture showed the highest phenolic content, followed by cell suspension culture. Compared with wild plants, in vitro cultures had lower levels of phenolic compounds, but some compounds not found in wild plants were detected in the in vitro cultures.
Article
Plant Sciences
Nishi Mishra, Manoj Kumar Tripathi, Sushma Tiwari, Niraj Tripathi, Swapnil Sapre, Ashok Ahuja, Sharad Tiwari
Summary: This experiment explored somaclonal variation as a method to increase drought tolerance in soybean. By treating calli/cell clumps/embryoids, drought-tolerant plantlets were successfully regenerated, confirming the presence of variability through random decamer primers.
Article
Agricultural Engineering
Li Li, Shan Li, Zhirong Cui, Yingying Wang, Yongyi Li, Lingyi Kong, Jun Luo
Summary: The study successfully developed Sarcandra glabra callus culture for in vitro production of sesquiterpenoids, laying the foundation for further research and utilization of S. glabra.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Biochemistry & Molecular Biology
Eda Zekai, Emine Acar, Dicle Donmez, Ozhan Simsek, Yildiz Aka Kacar
Summary: This study investigated the micropropagation and rooting performance of Azman and Grand Naine banana cultivars under in vitro drought stress conditions. The expression levels of four different genes of the CDPK gene family were determined and found to play a significant role in the response of banana plants to drought stress.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Plant Sciences
Yulia Yugay, Zhargalma Tsydeneshieva, Tatiana Rusapetova, Olga Grischenko, Anastasia Mironova, Dmitry Bulgakov, Vladimir Silant'ev, Galina Tchernoded, Victor Bulgakov, Yury Shkryl
Summary: EVs have been isolated and characterized from callus culture of Arabidopsis thaliana, showing similarities with intact plants in terms of protein and microRNA composition.
Article
Plant Sciences
Yang Liu, Naifu Zhou, Chengrui Luo, Qi Zhang, Peng Sun, Jianmin Fu, Shuzhan Li, Ze Li
Summary: This study examined the effects of different plant growth regulators and concentrations on tissue culture of persimmon trees and found the optimal conditions for germination of young embryos, induction of leaf callus, differentiation of adventitious shoots, and rooting culture.
Article
Biochemistry & Molecular Biology
Konstantin V. Kiselev, Olga A. Aleynova, Zlata V. Ogneva, Andrey R. Suprun, Alexandra S. Dubrovina
Summary: The study analyzed the expression of two widely used plant transgenes regulated by a double CaMV 35S promoter in Arabidopsis thaliana under different conditions. The results showed that transcriptional activity can vary significantly depending on plant organ, physiological conditions, and response to abiotic stress, highlighting the importance of attention to transgenic plant material and incubation conditions for ensuring high and stable transgene activity.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Konstantin V. Kiselev, Olga A. Aleynova, Zlata V. Ogneva, Andrey R. Suprun, Alexey A. Ananev, Nikolay N. Nityagovsky, Alexandra S. Dubrovina
Summary: This study conducted a comprehensive analysis of the expression of CaM and CML genes in highly resistant grapevine Vitis amurensis in response to stress hormones, phenolic precursors, and ultraviolet irradiation. The results showed that MeJA and SA significantly regulated the expression of VaCML genes, while UV-C irradiation had a notable impact on VaCML gene expression.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Plant Sciences
Konstantin Kiselev, Andrey R. Suprun, Olga A. Aleynova, Zlata Ogneva, Alexandra S. Dubrovina
Summary: Recent studies have shown that foliar application of dsRNA or siRNA can trigger RNAi-mediated silencing of specific genes in plant pathogens. However, the efficiency of gene silencing can be influenced by physiological conditions, plant age, time of day, and soil moisture. Optimal conditions for exogenously induced gene silencing in Arabidopsis thaliana include late application of dsRNA at night, low soil moisture, and specific application methods like brushing, spraying, and pipetting.
Article
Plant Sciences
Z. Ogneva, O. A. Aleynova, A. R. Suprun, Y. A. Karetin, A. S. Dubrovina, K. Kiselev
Summary: This study demonstrated that overexpression of VaSTS1 and VaSTS7 genes significantly increased plant tolerance to UV-B and UV-C in A. thaliana plants, while not affecting tolerance to other abiotic stresses. Overexpression of VaSTS1 gene led to the highest levels of trans-piceid and trans-resveratrol in transgenic plants.
BIOLOGIA PLANTARUM
(2021)
Article
Biochemistry & Molecular Biology
Andrey R. Suprun, Alexandra S. Dubrovina, Alexey P. Tyunin, Konstantin Kiselev
Summary: This study analyzed phenolic compounds in Russian wines for the first time and found that red wines contain more stilbenes, phenolic acids, and flavonols than white wines, with young red wines being a rich source of phenolic compounds.
Article
Biochemistry & Molecular Biology
Konstantin V. Kiselev, Andrey R. Suprun, Olga A. Aleynova, Zlata V. Ogneva, Alexander V. Kalachev, Alexandra S. Dubrovina
Summary: This study successfully regulated the CHS gene and anthocyanin accumulation in Arabidopsis through exogenous dsRNA treatments, and induced relevant biochemical changes by targeting the MYBL2 and ANAC032 genes. The results demonstrate that dsRNAs can efficiently downregulate Arabidopsis genes and induce biochemical changes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Olga A. Aleynova, Andrey R. Suprun, Nikolay N. Nityagovsky, Alexandra S. Dubrovina, Konstantin V. Kiselev
Summary: This study demonstrates that plant endophytes play a significant role in stimulating stilbene production in grape cell suspension, with endophytic fungi showing a more pronounced induction effect on stilbene accumulation compared to endophytic bacteria. The increase in stilbene content was due to the activation of phenylalanine ammonia lyase (PAL) and stilbene synthase (STS) gene expression. The findings suggest that grape endophytes could potentially serve as stimulators for stilbene biosynthesis in grapevine or grape cell cultures.
Article
Plant Sciences
Olga A. Aleynova, Andrey R. Suprun, Alexey A. Ananev, Nikolay N. Nityagovsky, Zlata V. Ogneva, Alexandra S. Dubrovina, Konstantin V. Kiselev
Summary: This study investigated the effect of overexpression of VaCML genes on stilbene and biomass accumulation in Vitis amurensis cell cultures. The results showed that overexpression of VaCML65 gene significantly increased stilbene content, while overexpression of other VaCML genes had inconsistent effects. The VaCML65 gene is suggested to be a strong positive regulator in the stilbene biosynthesis pathway, which could be useful for obtaining grape cultivars with high stilbene content and stress resistance in viticulture and winemaking.
Article
Plant Sciences
Olga A. Aleynova, Nikolay N. Nityagovsky, Alexandra S. Dubrovina, Konstantin Kiselev
Summary: This study analyzed the composition of bacterial endophytes in wild Amur grape in the south of the Russian Far East using two different methods. The results showed that the dominant endophytes in V. amurensis were Gammaproteobacteria, Alphaproteobacteria, Actinobacteria, and Bacilli. NGS revealed a greater diversity of classes and genera compared to the cultivation-dependent method. The study also found that the composition of endophytic bacteria varied depending on the plant organ, grape individuals, environmental conditions, and sampling time.
Article
Plant Sciences
Konstantin Kiselev, Andrey R. Suprun, Olga A. Aleynova, Zlata Ogneva, Eduard Y. Kostetsky, Alexandra S. Dubrovina
Summary: Recent investigations have shown that exogenous double-stranded RNAs (dsRNAs) can specifically suppress transgene expression in plants, indicating the potential of using dsRNAs as a promising strategy for plant gene regulation.
Article
Plant Sciences
Olga A. Aleynova, Nikolay N. Nityagovsky, Andrey R. Suprun, Alexey A. Ananev, Alexandra S. Dubrovina, Konstantin Kiselev
Summary: This study investigated the fungal endophytic community of wild grape Vitis amurensis using two different approaches. The results showed a diverse range of fungal taxa, with Dothideomycetes and Tremellomycetes being the most represented classes.
Editorial Material
Plant Sciences
Olga A. A. Aleynova, Konstantin V. V. Kiselev
Article
Agricultural Engineering
Yahui Bo, Shanshan Wang, Feifei Ma, Artem Yurevich Manyakhin, Guilin Zhang, Xiaohui Li, Chengxu Zhou, Baosheng Ge, Xiaojun Yan, Roger Ruan, Pengfei Cheng
Summary: Spermidine supplementation promotes cell proliferation and fucoxanthin synthesis in Isochrysis sp., especially under low light intensity.
BIORESOURCE TECHNOLOGY
(2023)
Review
Agricultural Engineering
Shengzhou Shan, Artem Yurevich Manyakhin, Chun Wang, Baosheng Ge, Jichang Han, Xuezhi Zhang, Chengxu Zhou, Xiaojun Yan, Roger Ruan, Pengfei Cheng
Summary: This paper reviews the carbon and energy metabolism of microalgae and reveals the synergistic mechanisms of mixotrophic mode. It explains the shortcomings of photoautotrophic and heterotrophic growth and proposes strategies to improve mixotrophic culture. The findings have important implications for increasing biomass production and improving microalgal culture.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Olga A. Aleynova, Konstantin V. Kiselev, Andrey R. Suprun, Alexey A. Ananev, Alexandra S. Dubrovina
Summary: CMLs are important plant calcium sensor proteins that sense and decode changes in intracellular calcium concentration. This study found that VaCML92 plays a positive regulatory role in the rapid response to cold stress and stress resistance in Vitis amurensis, as well as increased stilbene production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
