Review
Plant Sciences
Renatha Tavares de Oliveira, Joana Paula da Silva Oliveira, Andrea Furtado Macedo
Summary: This review article discusses the importance of the vanilla crisis and the potential of vanilla crop wild relatives as alternatives. The metabolic characterization and potential flavor compounds of these wild relatives are highlighted.
Article
Plant Sciences
Mani Manokari, Selvam Priyadharshini, Mathiyazhagan Cokulraj, Abhijit Dey, Mohammad Faisal, Abdulrahman Abdullah Alatar, Anshu Alok, Mahipal Singh Shekhawat
Summary: This study evaluated the effect of silver nitrate on direct and indirect somatic embryogenesis in Vanilla planifolia. The results showed that silver nitrate promoted somatic embryogenesis and plantlet regeneration in Vanilla planifolia.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Article
Biochemical Research Methods
Zhenjie Zhao, Liwei Hu, Qiansi Chen, Huaxin Dai, Xiangyu Meng, Qisheng Yin, Taibo Liang
Summary: This study investigates the regulatory mechanism of carbon nanoparticles (CNPs) on tobacco callus growth at the protein level. Proteomic profiling reveals that CNPs enhance the growth of tobacco callus by affecting differentially expressed proteins located in mitochondria and involved in calcium-mediated signaling pathway.
PLANT MOLECULAR BIOLOGY REPORTER
(2022)
Article
Chemistry, Multidisciplinary
Mi Li, Yunqiao Pu, Xianzhi Meng, Fang Chen, Richard A. Dixon, Arthur J. Ragauskas
Summary: This study revealed a particularly high level of tricin-lignin in vanilla plant, especially in the aerial roots, which could play a vital role in valorization of lignin and offer new ways to design and engineer lignin structures for improved plant properties.
Article
Plant Sciences
Kah-Lok Thye, Wan Muhamad Asrul Nizam Wan Abdullah, Janna Ong-Abdullah, Dhilia Udie Lamasudin, Chien-Yeong Wee, Mohd Hafis Yuswan Mohd Yusoff, Jiun-Yan Loh, Wan-Hee Cheng, Kok-Song Lai
Summary: The utilization of calcium lignosulfonate (CaLS) improves shoot multiplication in Vanilla planifolia. In this study, the underlying mechanisms of CaLS in promoting shoot multiplication were investigated through proteomics, biochemical assays, and nutrient analysis. The results showed that CaLS treatment enhanced various cellular metabolisms, increased enzyme activity and content of carbohydrates and proteins, and elevated nutrient ion levels, especially calcium and sodium ions. Additionally, CaLS was found to regulate cellular homeostasis through the modulation of signaling molecules. These findings demonstrated that CaLS treatment stimulates nutrient uptake, induces cell metabolism, and regulates cell homeostasis to enhance shoot multiplication in V. planifolia.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2023)
Review
Horticulture
Camelia Sava Sand, Maria-Mihaela Antofie
Summary: This article reviews new scientific achievements on Syngonium species and related topics, and discusses the principles and stages required to start an industrial-level tissue culture protocol. Different media compositions induce different morphogenetic responses, particularly in relation to the development of xylematic elements. The formation of protocorms is highly dependent on the mineral composition of the culture medium and plant growth regulators.
Article
Plant Sciences
Sharmilla Ashokhan, Saiful Anuar Karsani, Jamilah Syafawati Yaacob
Summary: Azadirachta indica or neem is known for its insecticidal and therapeutic properties. However, the proteomics response to abiotic stresses in this plant has not been studied. This research compared different protein extraction methods and found that TCA-acetone with PVPP was the most effective. Differential proteomics analysis showed that proteins related to oxidoreduction, energy, transcription, stress response, respiration, and cell division were differentially regulated under salinity stress. Furthermore, the study revealed the impact of JA stress on protein expression. This research provides valuable insights into sustainable production of bioactive constituents and the mechanisms of stress tolerance in neem.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Plant Sciences
Jorge Warner, Yesly Camacho-Solis, Victor M. Jimenez
Summary: This study describes direct and indirect shoot regeneration in Vanilla odorata and indirect regeneration in V. pompona for the first time. The results show that stem nodal segments and callus from both species have high regenerative potential under different culture conditions. Histological analysis also reveals the presence of calcium oxalate raphides in the callus. The findings of this study provide a foundation for large-scale propagation efforts and other biotechnological applications of Vanilla odorata and V. pompona.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
(2023)
Article
Multidisciplinary Sciences
Eduardo Martinez-Santos, Carlos Alberto Cruz-Cruz, Jose Luis Spinoso-Castillo, Jerico Jabin Bello-Bello
Summary: The study focused on evaluating the morphological, physiological, and biochemical responses of Vanilla planifolia under in vitro water stress conditions induced with polyethylene glycol (PEG). It was found that increasing PEG concentration led to a decrease in growth and chlorophyll content, while dry matter, proline, and glycine betaine contents increased. The results suggest that PEG-induced osmotic stress can help in understanding the physiological and biochemical mechanisms of response to water stress, and Pro and GB osmolytes can be used as potential markers in breeding programs for water stress tolerance.
SCIENTIFIC REPORTS
(2021)
Article
Horticulture
Maria Karen Serrano-Fuentes, Fernando Carlos Gomez-Merino, Serafin Cruz-Izquierdo, Jose Luis Spinoso-Castillo, Jerico Jabin Bello-Bello
Summary: In this study, somaclonal variation in Vanilla planifolia was induced by gamma radiation and detected using ISSR molecular markers. Results showed that low doses of radiation promoted development, while high doses inhibited growth and caused death. UBC-808, UBC-836, and UBC-840 primers exhibited the highest percentage of polymorphism. Genetic distance analysis revealed the presence of somaclonal variation in both irradiated and non-irradiated explants.
Article
Ecology
Ireri A. Carbajal-Valenzuela, Ariel H. Munoz-Sanchez, Juan Hernandez-Hernandez, Francisco Barona-Gomez, Camille Truong, Angelica Cibrian-Jaramillo
Summary: The study investigated the differences in endophytic microbiomes between symptomatic and asymptomatic vanilla plants, showing the presence of specific core microbiota in roots and stems. However, there were no significant differences in microbial community richness among feral, asymptomatic, and symptomatic cultivated vanilla. The findings lay the groundwork for a better understanding of the role of the microbiome in vanilla fungal diseases.
Article
Plant Sciences
Lucas Rodrigues Xavier, Caio Cezar Guedes Correa, Roberta Pena da Paschoa, Karina da Silva Vieira, Daniel Dastan Rezabala Pacheco, Lucas do Espirito Santo Gomes, Barbara Cardoso Duncan, Lais dos Santos da Conceicao, Vitor Batista Pinto, Claudete Santa-Catarina, Vanildo Silveira
Summary: In this study, the proteome of C. papaya callus was analyzed to identify key proteins involved in embryogenic callus formation. The results showed that proteins related to seed maturation and storage decrease, while carbohydrate and amino acid metabolism, aerobic respiration, and protein catabolism increase during callus induction. Protein kinases associated with auxin responses and regulatory proteins were enriched in the early stage of callus induction. Predicted protein-protein networks revealed the regulatory role of 14-3-3 proteins during callus induction and the association of proteins involved in oxidative phosphorylation and hormone response.
Review
Food Science & Technology
Charles Spence
Summary: Our food choices and consumption behaviors are often influenced by the pleasantness of odors, especially those related to food. The origins of odor pleasantness have been explained by associative learning, innate factors, and molecular mechanisms. This article explores the origins of the pleasant response to vanilla, a universally liked smell, and discusses its historical use in food and medicine. The potential use of "sweet smells" to maintain sweetness in commercial food products is also discussed, with artificial vanilla flavoring being a cost-effective alternative to natural vanilla pods.
Article
Chemistry, Multidisciplinary
Brendan G. Dwyer, Chao Wang, Daniel Abegg, Brittney Racioppo, Nan Qiu, Zhensheng Zhao, Dany Pechalrieu, Anton Shuster, Dominic G. Hoch, Alexander Adibekian
Summary: This study introduces arylazopyrazole ureas and sulfones as a new class of photoswitchable serine hydrolase inhibitors and presents a chemoproteomic platform for rapid discovery of optically controlled serine hydrolase targets in complex proteomes. The research identifies highly potent and selective photoswitchable inhibitors for drug-metabolizing enzymes, and demonstrates their pharmacological application in optically controlling drug metabolism. The study suggests the potential for using photopharmacological tools to modulate drug metabolism by controlling the activity of metabolizing enzymes, and offers synthetically accessible scaffolds for expanding to other serine hydrolases.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Food Science & Technology
Oscar Antonio-Gutierrez, Isidro Pacheco-Reyes, Luicita Lagunez-Rivera, Rodolfo Solano, Maria del Pilar Canizares-Macias, Gerard Vilarem
Summary: This research evaluated the application of microwave and ultrasound during the killing stage of the Vanilla planifolia pod curing process and its effect on vanillin content and beta-glucosidase activity. The results show that the application of these technologies significantly improves vanillin yield and reduces the curing time to 20 days.
Article
Multidisciplinary Sciences
Muhammad Asyraf Mohd Amnan, Teen-Lee Pua, Su-Ee Lau, Boon Chin Tan, Hisateru Yamaguchi, Keisuke Hitachi, Kunihiro Tsuchida, Setsuko Komatsu
Summary: Nitric oxide plays a diverse role in plant growth and defensive responses, and this study demonstrates that treatment with sodium nitroprusside (SNP) can improve plant growth and reduce yellowing leaves under osmotic stress induced by polyethylene glycol (PEG) in banana roots. Proteins related to carbohydrate and energy metabolism were found to be differentially expressed in SNP-treated banana roots compared to PEG-treated banana roots, suggesting a potential link between nitric oxide-induced osmotic stress tolerance and improved carbohydrate and energy metabolism capability in higher plants.
Review
Plant Sciences
Su-Ee Lau, Mohd Fadhli Hamdan, Teen-Lee Pua, Noor Baity Saidi, Boon Chin Tan
Summary: The role of NO in drought stress still lacks knowledge and faces technical challenges, with future research recommendations focusing on exploring this area further. The modulation of NO production to alleviate abiotic stress disturbances highlights the potential of genetic manipulation to improve plant fitness under adverse growth conditions.
Article
Plant Sciences
Muhammad Asyraf Mohd Amnan, Wan Mohd Aizat, Fiqri Dizar Khaidizar, Boon Chin Tan
Summary: Drought poses a significant threat to the agricultural sector, with limited knowledge on plant responses to drought stress and recovery. Pandanus amaryllifolius, a moderate drought-tolerant plant, displayed changes in morpho-physiological, biochemical, and protein levels in response to drought stress. The study revealed alterations in leaf relative water content, chlorophyll content, electrolyte leakage, proline and malondialdehyde contents, and antioxidant enzyme activities in drought-stressed and recovered P. amaryllifolius plants, with significant protein changes related to carbon metabolism, photosynthesis, stress response, and antioxidant activity.
Review
Biotechnology & Applied Microbiology
Sheue Ni Ong, Boon Chin Tan, Aisyafaznim Al-Idrus, Chee How Teo
Summary: This article discusses the challenges and time-consuming nature of gene prediction, and introduces the methods of identifying small open reading frames (sORFs) in plant genomes using sequencing technologies and bioinformatics tools. Additionally, the article provides an overview of the classification, functions, and potential applications of plant sORFs in crop improvement.
Review
Plant Sciences
Mohd Fadhli Hamdan, Siti Nurfadhlina Mohd Noor, Nazrin Abd-Aziz, Teen-Lee Pua, Boon Chin Tan
Summary: The evolution of technological applications in agriculture, including genetic engineering and genome editing techniques, is crucial for increasing crop yields and quality, addressing climate change and rising food demand. However, the development and application of these modern technologies also face challenges in regulation and standardization.
Article
Plant Sciences
Ee Yang Teoh, Chee How Teo, Nadiya Akmal Baharum, Teen-Lee Pua, Boon Chin Tan
Summary: Flooding caused by climate change poses a threat to plant growth and food production worldwide. However, the lack of knowledge on how crops respond to flooding stress hinders efforts to enhance productivity. In this study, we investigated the responses of banana plants to soil waterlogging and found that waterlogging induced the formation of adventitious roots and aerenchyma. Additionally, the antioxidant activities and levels of hydrogen peroxide and malondialdehyde in waterlogged bananas increased. Transcriptome analysis revealed differential expression of a total of 3508 unigenes in banana roots under 1-day waterlogging conditions, which are involved in abiotic stress response, signal transduction, and carbohydrate metabolism. These findings provide valuable insights into the molecular events of bananas in response to waterlogging stress and can aid in the development of waterlogging-tolerant crops in the future.
Article
Biology
Chou Khai Soong Karlson, Siti Nurfadhlina Mohd Noor, Norzulaani Khalid, Boon Chin Tan
Summary: Flavonoids, a crucial class of natural compounds found in plants, have limited industrial applications due to their low abundance in nature. This study aimed to enhance flavonoid production by silencing the C4H enzyme using CRISPRi technology. Results showed that silencing C4H led to higher accumulation of flavonoid substrate, providing valuable insight for future development of CRISPRi-based metabolic engineering in plants.
Review
Plant Sciences
Mohd Fadhli Hamdan, Chou Khai Soong Karlson, Ee Yang Teoh, Su-Ee Lau, Boon Chin Tan
Summary: Climate change poses a serious threat to global agriculture and food production. Plant genome editing technologies, particularly the CRISPR/Cas system, offer targeted alterations of the plant genome to develop crops with desired traits and mitigate the impacts of biotic and abiotic stresses on agriculture. This review discusses the current development, future applications, shortcomings, and challenges of genome editing technologies in agriculture.
Editorial Material
Plant Sciences
Mohd Fadhli Hamdan, Goetz Hensel, Anshu Alok, Boon Chin Tan
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Su-Ee Lau, Teen-Lee Pua, Noor Baity Saidi, Janna Ong-Abdullah, Dhilia Udie Lamasudin, Boon Chin Tan
Summary: This study investigated the morphological and protein responses of banana plants to drought stress and post-drought recovery. The results showed that drought significantly reduced various growth parameters and induced oxidative stress in the banana plants. However, these effects were reversible after recovery from drought stress. Analysis of the proteome identified differentially abundant proteins related to various metabolic and biological processes.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Plant Sciences
Muhammad Asyraf Mohd Amnan, Wee Fei Aaron Teo, Wan Mohd Aizat, Fiqri Dizar Khaidizar, Boon Chin Tan
Summary: Wood vinegar, a sustainable alternative, has been shown to promote plant growth and enhance stress tolerance. This study evaluated the efficacy of oil palm wood vinegar (OPWV) in mitigating the adverse effects of drought stress on Pandanus amaryllifolius. The results demonstrated that applying OPWV at a specific concentration and frequency increased the plant's tolerance to drought stress.
Article
Agriculture, Multidisciplinary
Nur Asniyati Abdul Halim, Boon Chin Tan, Norzulaani Khalid, Jamilah Syafawati Yaacob
Summary: Plant tissue culture is an efficient biotechnological tool for mass producing high quality crops, but it can lead to somaclonal variation caused by genetic and/or epigenetic modulations. This study investigated the role of histone deacetylation in dwarfism of tissue culture-derived pineapple plants through overexpression of the AtHD2 gene. The results showed that HDACs play an important role in the response of pineapple plants to abiotic stress, and this phenomenon is epigenetically regulated.
Article
Microbiology
Ahmad Zuhairi Abdul Malek, Nur Amalina Ahmed Lokman, Boon Chin Tang, Yin Sze Lim
Summary: This study used a machine learning-based NeuBI prediction approach to predict bacteriocin-and probiotic-associated genes in Weissella cibaria NM1. In vitro analysis confirmed the antimicrobial and probiotic properties of the strain, showing promising results for potential probiotic applications.
MALAYSIAN JOURNAL OF MICROBIOLOGY
(2021)
Article
Microbiology
Suffi Nurul Husna Suffian, Boon Chin Tang, Yin Sze Lim
Summary: This study demonstrated the synergistic effect of bacteriocin produced by Pediooccus pentosaceus TU2 in combination with conventional antibiotics against Pseudomonas aeruginosa, leading to reduced minimum inhibitory concentration and enhanced bactericidal effect. Scanning electron microscope results indicated that bacteriocin TU2 might enhance membrane permeability of antibiotics and cause cell death, suggesting a promising strategy in combating P. aeruginosa infections.
MALAYSIAN JOURNAL OF MICROBIOLOGY
(2021)
Review
Computer Science, Information Systems
Clarence Augustine T. H. Tee, Yun Xin Teoh, Por Lip Yee, Boon Chin Tan, Khin Wee Lai
Summary: Early detection of Ganoderma disease is crucial for proper treatment and disease control. Research shows that the combination of remote sensors and machine learning techniques can accurately identify the disease, reducing costs and time consumption. Support vector machine (SVM) is suggested as the best classifier, while potential machine learning techniques like ANN, CNN, and GAN could enhance the detection system.
