Article
Environmental Sciences
Naeun Kim, Yongtae Ahn, Jungman Jo, Heesoo Pyo, Jeongae Lee, Jaeyoung Choi
Summary: This study investigated the effects of accidental contamination of soils with various chemicals, and found that phytosphingosine and Sphingomonas could potentially serve as biomarkers to evaluate soil contamination. Rain played an important role in the recovery of microbial and metabolic profiles after chemical accidents.
Article
Biochemistry & Molecular Biology
Minnie Jacob, Refat M. Nimer, Mohamad S. Alabdaljabar, Essa M. Sabi, Mysoon M. Al-Ansari, Maged Housien, Khalid M. Sumaily, Lina A. Dahabiyeh, Anas M. Abdel Rahman
Summary: This study used liquid chromatography-mass spectrometry (LC-MS) metabolomics to analyze the metabolome of serum from patients with nephrotic syndrome (NS) and found significant changes in 176 metabolites compared to the control group. Dysregulation of arginine, proline, and tryptophan metabolism, as well as arginine, phenylalanine, tyrosine, and tryptophan biosynthesis, were the most common metabolic pathways affected in NS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Meilin Ding, Zha Zhen, Mei Ju, Suolang Quzong, Xuesi Zeng, Xiaoxia Guo, Rui Li, Mingming Xu, Jingjing Xu, Hongyang Li, Wei Zhang
Summary: Our study found significant differences in the metabolomic profiles between vitiligo patients and healthy subjects in both plateau and plain areas, with significantly higher levels of S1P in the serum of vitiligo patients and healthy subjects in the plateau area. These findings suggest that alterations in S1P metabolism may be involved in the pathogenesis of vitiligo.
CLINICAL IMMUNOLOGY
(2023)
Article
Parasitology
Mingxing Zhu, Xiancai Du, Hongxia Xu, Songhao Yang, Chan Wang, Yazhou Zhu, Tingrui Zhang, Wei Zhao
Summary: Metabolomics approach revealed that the metabolism of nucleotides, alkaloids, amino acids, amides, and organic acids in mice is closely interrelated with E. granulosus infection. The study found that metabolic pathways in the liver such as tyrosine and tryptophan biosynthesis, phenylalanine, valine, leucine and isoleucine biosynthesis, and phenylalanine metabolism were notably associated with the occurrence and development of hydatid disease.
PARASITES & VECTORS
(2021)
Article
Dermatology
Qiying Xiong, Daoqing Zhong, Qian Li, Yihui Yu, Sanquan Zhang, Jingyao Liang, Xibao Zhang
Summary: Recent studies have found that certain metabolites in the blood and urine are involved in the development of psoriasis, but the research on skin metabolomics for psoriasis is limited. In this study, researchers compared the metabolic profiles of lesional and nonlesional skin from 12 psoriasis patients using liquid chromatography-mass spectrometry. They identified several metabolites that were significantly different between lesional and nonlesional skin, mainly related to amino acid, lipid, and nucleotide metabolism. Fourteen potential biomarkers were identified, some of which were correlated with disease severity.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Food Science & Technology
Dhara Dixit
Summary: This study reveals the biochemical traits, mineral makeup, and secondary metabolite outline of Iyengaria stellata using untargeted metabolite profiling. A total of 108 putative metabolites were detected, including steroids, terpenoids, ketones, esters, polyphenols, anthraquinones, tocopherol, etc. The study highlights the potential of I. stellata as a natural source in pharmaceutical and nutraceutical compositions.
Article
Physiology
Eden A. Houske, Matthew G. Glimm, Annika R. Bergstrom, Sally K. Slipher, Hope D. Welhaven, Mark C. Greenwood, Greta M. Linse, Ronald K. June, Alan S. L. Yu, Darren P. Wallace, Alyssa K. Hahn
Summary: This study analyzed the urine metabolites of early-stage ADPKD patients and identified metabolic pathway alterations and candidate diagnostic and prognostic biomarkers. The results provide potential therapeutic targets and biomarkers for early diagnosis and tracking disease progression of ADPKD.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Shreyas Deshpande, Sirsha Mitra
Summary: Beta-cyclocitral (beta CC) is an important oxidative product of beta-carotene that enables plants to acclimate to multiple stresses while maintaining growth. This study demonstrates the metabolic changes induced by beta CC that promote defense and growth in tomato plants under stress. The findings show that beta CC specifically affects amino acid and phenolic metabolism in tomato plants.
Article
Biochemical Research Methods
Juan J. Aristizabal-Henao, Dominick J. Lemas, Fmily K. Griffin, Kaylie Anne Costa, Camden Camacho, John A. Bowden
Summary: The number of metabolomics studies has increased significantly in recent years, with advancements in analyte separation techniques and databases playing a major role in this growth. Establishing baseline metabolomics data using complementary data acquisition strategies can help address challenges in comparing data across studies. This study provides valuable nontargeted metabolomics data for various human reference materials, highlighting significant differences between sample types and supporting the use of nontargeted strategies in global metabolomics.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Biochemistry & Molecular Biology
Zhanxuan E. Wu, Marlena C. Kruger, Garth J. S. Cooper, Ivana R. Sequeira, Anne-Thea McGill, Sally D. Poppitt, Karl Fraser
Summary: Untargeted metabolomics of blood samples has been widely used to study metabolic alterations in diseases and identify biomarkers. This study found that the concentration of metabolites in plasma was more reflective of the liver profile than muscle or adipose tissue, highlighting the importance of considering the metabolomic relationship between different tissues and plasma.
Article
Multidisciplinary Sciences
Changmeng Cui, Li Zhu, Qian Wang, Ruijuan Liu, Dadi Xie, Yujin Guo, Dingyi Yu, Changshui Wang, Dan Chen, Pei Jiang
Summary: The study investigated the metabolic changes after vancomycin administration in mice, revealing that vancomycin affects the metabolism in various organs, providing new insights for identification of vancomycin-induced toxicity.
Review
Psychiatry
Sylvana Vilca-Melendez, Malin V. Uthaug, Julian L. Griffin
Summary: While psychedelics show promise for treating mental health disorders, more research is needed to understand their biological effects and mechanisms of action. The use of metabonomics like H-1 NMR in psychedelic research is beneficial, but current studies highlight a gap in utilizing this technique effectively.
FRONTIERS IN PSYCHIATRY
(2021)
Article
Plant Sciences
Cyril Abadie, Julie Lalande, Guillaume Tcherkez
Summary: Plant metabolomics is widely used in plant physiology to study metabolic responses to environmental parameters. Derivatization followed by GC-MS metabolic profiling is a major technique for quantifying primary carbon, sulphur, and nitrogen metabolites. However, routine exact mass GC-MS analyses are not common due to the lack of dedicated databases. In this study, a curated database for exact mass metabolomics focused on primary metabolism was presented. The advantages of exact mass analyses were demonstrated, including resolving isotopic patterns, localizing S-containing metabolites, and avoiding identification errors. A practical example using Arabidopsis leaves showcased how exact mass GC-MS analysis can be used for plant samples and identify metabolic profiles.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Plant Sciences
Alison Green, Guillermo Federico Padilla-Gonzalez, Methee Phumthum, Monique S. J. Simmonds, Nicholas J. Sadgrove
Summary: Commercially used for treating chronic venous insufficiency, A. hippocastanum L. fruits contain beta-aescin which exhibits anti-inflammatory effects. However, diverse organ-specific secondary metabolites have been overlooked as potential pharmacological opportunities. Untargeted metabolomics analysis revealed unique chemical profiles in different organs of Aesculus plants, with fruits showing the highest antifungal and antioxidant activities. This suggests potential therapeutic leads for further exploration in the entire genus of Aesculus.
Article
Oncology
Xin Chen, Bo-lun Shi, Run-zhi Qi, Xing Chang, Hong-gang Zheng
Summary: This study investigated the therapeutic effects and molecular mechanisms of Honokiol (HNK) on colorectal cancer through metabolomics analysis. Results showed potential urine biomarkers related to purines, tyrosines, tryptophans metabolism, and indicated that HNK modulated the citrate cycle to exert its efficacy against intestinal tumors.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemical Research Methods
Xiaoxiao Wang, Fujian Zheng, Meizhen Sheng, Guowang Xu, Xiaohui Lin
Summary: To improve the accuracy of retention time (RT) prediction, this study proposes a method called multi-data combinations and adaptive neural network (MDC-ANN). MDC-ANN establishes the RT prediction model for a target chromatographic system using transfer learning and a deep learning model trained on a large dataset. Experimental results demonstrate that integrating multiple molecular representations provides more information, improves RT prediction performance, and contributes to compound annotation. Different chromatographic systems may require different molecular representation combinations for accurate RT prediction. Hence, MDC-ANN, which automatically determines the best combination of molecular representations for a specific system, shows promise for accurate RT prediction in real applications.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Miriam Hoene, Xinjie Zhao, Juergen Machann, Andreas L. L. Birkenfeld, Martin Heni, Andreas Peter, Andreas Niess, Anja Moller, Rainer Lehmann, Guowang Xu, Cora Weigert
Summary: Physical exercise is a powerful preventative measure for cardiometabolic diseases. The individual response to lifestyle interventions varies and cannot currently be predicted. Recent research has shown that Lac-Phe produced during exercise could contribute to weight loss and potentially explain differences in the effectiveness of exercise interventions in humans.
Article
Medical Laboratory Technology
Disheng Feng, Zixuan Wang, Hang Li, Xianzhe Shi, Lin Zou, Hongwei Kong, Zhiliang Xu, Chaowen Yu, Chunxiu Hu, Guowang Xu
Summary: This study developed a highly efficient method for rapid and accurate measurement of 17-alpha-hydroxyprogesterone (17-OHP) and related steroids in amount/volume-limited clinic samples, which is of great importance for the precise newborn diagnosis of congenital adrenal hyperplasia (CAH) and its subtypes in clinic.
CLINICA CHIMICA ACTA
(2023)
Article
Engineering, Environmental
Chang Wang, Huayu Fu, Jun Yang, Lei Liu, Fenghong Zhang, Chunyu Yang, Hongyuan Li, Jiamiao Chen, Qi Li, Xiaolin Wang, Yaorui Ye, Nan Sheng, Yong Guo, Jiayin Dai, Guowang Xu, Xinyu Liu, Jianshe Wang
Summary: A study found that PFECAs have inhibitory effects on cellular stress signals and lead to hepatomegaly in mice. Additionally, PFECAs also have inhibitory effects on glucocorticoid receptors, which may result in extensive suppression of stress signals. Unlike other similar substances, the next-generation replacements of legacy PFASs, PFECAs, may pose potential risks to human health.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Qingqing Wang, Miriam Hoene, Chunxiu Hu, Louise Fritsche, Robert Ahrends, Gerhard Liebisch, Kim Ekroos, Andreas Fritsche, Andreas L. Birkenfeld, Xinyu Liu, Xinjie Zhao, Qi Li, Benzhe Su, Andreas Peter, Guowang Xu, Rainer Lehmann
Summary: Reliability, robustness, and interlaboratory comparability of quantitative measurements in clinical lipidomics studies are crucial. The different ex vivo stability of lipids in blood poses a risk to data interpretation. Clear recommendations for blood sample collection are necessary. In this study, we used UHPLC-high resolution mass spectrometry to investigate the stability of 417 lipid species in EDTA whole blood under different temperature conditions and time points. Our data suggest that cooling whole blood immediately and continuously, and separating plasma within 4 hours, unless focusing on robust lipids, are recommended. Lists of lipids' ex vivo (in)stability and potential biomarkers in whole blood are provided. Overall, our results contribute to the international efforts in achieving reliable and comparable clinical lipidomics studies in the future.
JOURNAL OF LIPID RESEARCH
(2023)
Article
Chemistry, Analytical
Xingyu Guo, Lina Zhou, Yi Wang, Feng Suo, Chuanxia Wang, Wei Zhou, Lingshan Gou, Maosheng Gu, Guowang Xu
Summary: This study developed a LC-MS-based method for neonatal metabolomics analysis of DBS and investigated the influences of blood volume and chromatographic effects on metabolite levels. The method showed good repeatability, precision, and linearity, and was applied to study metabolic disruptions of congenital hypothyroidism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemistry & Molecular Biology
Xiaoshan Sun, Zhen Jia, Yuqing Zhang, Xinjie Zhao, Chunxia Zhao, Xin Lu, Guowang Xu
Summary: Direct infusion nanoelectrospray high-resolution mass spectrometry (DI-nESI-HRMS) is a promising tool for high-throughput metabolomics analysis. To overcome the limitations of metabolite assignment, a serum metabolome characterization method using a reaction network combined with mass accuracy and isotopic patterns filter was proposed. The approach was validated and compared to database search, showing improved coverage and accuracy rates. The method was then applied to a case study in diabetes metabolomics.
Article
Nanoscience & Nanotechnology
Ruibin Qiang, Haofei Huang, Jia Chen, Xianzhe Shi, Zengjie Fan, Guowang Xu, Hongdeng Qiu
Summary: Rheumatoid arthritis (RA) is characterized by inflammation and pain in the joints, caused in part by a lack of lubrication and excessive proinflammatory proteins. Researchers have developed two potential nanoagents, derived from herbal medicine, that offer both improved joint lubrication and anti-inflammatory effects, showing promise for treating RA.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Analytical
Xiaoshan Sun, Yueyi Xia, Xinjie Zhao, Xinxin Wang, Yuqing Zhang, Zhen Jia, Fujian Zheng, Zaifang Li, Xiuqiong Zhang, Chunxia Zhao, Xin Lu, Guowang Xu
Summary: Direct-infusion Fourier transform ion cyclotron resonance mass spectrometry (DI-FTICR MS) is a promising method for metabolomic analysis with ultrahigh mass accuracy and resolution. In this study, a novel deep characterization approach of serum metabolome was proposed using a segment-optimized spectral-stitching DI-FTICR MS method. This method combines high-confidence and database-independent formula assignments to achieve highly efficient acquisition and accurate assignment of thousands of features in a pooled human serum sample.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Xinxin Wang, Chao Li, Zaifang Li, Yanpeng Qi, Xiuqiong Zhang, Xinjie Zhao, Chunxia Zhao, Xiaohui Lin, Xin Lu, Guowang Xu
Summary: In this study, a structure-guided molecular network strategy (SGMNS) is proposed for deep annotation of untargeted metabolomics data. The strategy utilizes a global connectivity molecular network (GCMN) constructed based on molecular fingerprint similarity of chemical structures in metabolome databases. SGMNS performs network annotation propagation using known metabolites as seeds and achieves high annotation accuracy and precision.
ANALYTICAL CHEMISTRY
(2023)
Article
Environmental Sciences
Yuting Wang, Lina Zhou, Tiantian Chen, Lei You, Xianzhe Shi, Xinyu Liu, Sijia Zheng, Jie Jiang, Yuebin Ke, Guowang Xu
Summary: In this study, a 2D-LC-HRMS-based screening strategy was developed for simultaneously screening pesticides, veterinary/human drugs, and other chemical pollutants in serum. The method was validated to detect 92% and 81% of 1022 residues spiked in serum at concentrations of 50 ng/mL and 5 ng/mL, respectively. This strategy is helpful for studying the effect of exogenous exposures on human health.
ENVIRONMENTAL POLLUTION
(2023)
Article
Biochemical Research Methods
Xiaoyu Xie, Liangqiang Chen, Tiantian Chen, Fan Yang, Zixuan Wang, Yang Hu, Jianjun Lu, Xin Lu, Qi Li, Xiuqiong Zhang, Ming Ma, Li Wang, Chunxiu Hu, Guowang Xu
Summary: This study presents a chlorine isotope labeling-assisted ultrahigh-performance liquid chromatography-high resolution mass spectrometry-based method for profiling and annotating carbonyl compounds in sauce flavored-Baijiu Daqu. A total of 487 carbonyl compounds were detected, among which 314 (64.5%) were positively or putatively identified. The method provides an effective and economical strategy for comprehensive analysis of carbonyl compounds in complex matrix samples.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Cardiac & Cardiovascular Systems
Gaokun Qiu, Yuhui Lin, Yang Ouyang, Mingrong You, Xinjie Zhao, Hao Wang, Rundong Niu, Wending Li, Xuedan Xu, Qi Yan, Yurong Liu, Yingmei Li, Handong Yang, Xiulou Li, Meian He, Xiaomin Zhang, Xiao-Ou Shu, Guowang Xu, Tangchun Wu
Summary: This study aimed to identify metabolites associated with incident acute coronary syndrome (ACS) and explore the causality of these associations. Three metabolites were found to be associated with ACS risk, including one that is a degradation product of a gut-brain peptide, one that is a marker of short-term glycemic excursions, and one that is a very-long-chain saturated fatty acid. These findings highlight the importance of gastrointestinal hormones and glycemic fluctuations in the development of ACS.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2023)
Article
Immunology
Xinyu Liu, Congshu Xiao, Pengwei Guan, Qianqian Chen, Lei You, Hongwei Kong, Wangshu Qin, Peng Dou, Qi Li, Yanju Li, Ying Jiao, Zhiwei Zhong, Jun Yang, Xiaolin Wang, Qingqing Wang, Jinhui Zhao, Zhiliang Xu, Hong Zhang, Rongkuan Li, Peng Gao, Guowang Xu
Summary: To control the COVID-19 pandemic, efforts have been made to achieve herd immunity through vaccination since 2020. Unfortunately, most COVID-19 vaccines were approved without a thorough evaluation process. Metabolomic analysis can authentically assess the effects of vaccines by reflecting the responses to stimuli.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Gastroenterology & Hepatology
Runze Ouyang, Juan Ding, Yan Huang, Fujian Zheng, Sijia Zheng, Yaorui Ye, Qi Li, Xiaolin Wang, Xiao Ma, Yuxin Zou, Rong Chen, Zhihong Zhuo, Zhen Li, Qi Xin, Lina Zhou, Xin Lu, Zhigang Ren, Xinyu Liu, Petia Kovatcheva-Datchary, Guowang Xu
Summary: The gut microbiota plays a crucial role in maintaining host wellbeing by producing various metabolites. The assembly of the gut microbiome is influenced by postnatal factors, but little is known about the development of the gut metabolome. Our study reveals that geography has a significant impact on microbiome dynamics in the first year of life, with major compositional differences observed between Chinese and Swedish cohorts. We found that lipid metabolism, especially acylcarnitines and bile acids, is the most abundant metabolic pathway in the newborn gut, and delivery mode and feeding contribute to differences in the gut metabolome since birth.
