Article
Biochemistry & Molecular Biology
Huili Yang, Yadong Xue, Bing Li, Yanan Lin, Haochuan Li, Zhanyong Guo, Weihua Li, Zhiyuan Fu, Dong Ding, Jihua Tang
Summary: This study identified a chimeric mitochondrial gene (ATP6C) as the factor causing CMS in maize, and discovered its mechanism of action involving reduction in the quantity and activity of assembled F1Fo-ATP synthase, accumulation of excess protons in the mitochondrial inner membrane space, triggering ROS burst, premature programmed cell death of tapetal cells, and pollen abortion.
Article
Agronomy
Huadong Wang, Qing Xiao, Chao Wei, Hui Chen, Xiaohan Chen, Cheng Dai, Jing Wen, Chaozhi Ma, Jinxing Tu, Tingdong Fu, Jinxiong Shen, Bin Yi
Summary: By utilizing Brassica 60 K array, bulk segregant analysis, and map-based cloning, a mitochondria-localized pentatricopeptide repeat protein gene was identified and transferred into the hau CMS line, where it successfully restored fertility. The research showed that Rfh was highly expressed in flower buds, and the functional divergence between RFH and rfh may be attributed to 59 amino acid residue differences in the deduced protein sequences.
THEORETICAL AND APPLIED GENETICS
(2021)
Article
Agronomy
Xiaolong Fang, Yanyan Sun, Jinhong Li, Meina Li, Chunbao Zhang
Summary: Hybrid breeding is important for meeting the challenge of feeding a growing world population with limited agricultural land. There have been significant advancements in soybean genome and genetic studies, but commercialization of hybrid soybean is still in progress. This review summarizes recent progress in male sterility-based breeding programs and the current status of hybrid soybean breeding. Efforts have been made to identify male-sterile lines in soybean and over 40 hybrid soybean varieties have been developed. Increasing the out-crossing rate in an economical way is crucial for accelerating hybrid soybean breeding.
MOLECULAR BREEDING
(2023)
Article
Biochemistry & Molecular Biology
Senlin Xiao, Wei Song, Jinfeng Xing, Aiguo Su, Yanxin Zhao, Chunhui Li, Zi Shi, Zhiyong Li, Shuai Wang, Ruyang Zhang, Yuanrong Pei, Huabang Chen, Jiuran Zhao
Summary: Moderate stimuli in mitochondria can enhance stress adaptability in animals, and this study explores whether mitochondria play similar roles in plants. The results show that the moderate expression of the sterilizing gene ORF355 in CMS-S maize leads to enhanced stress adaptability, with improved growth potential and higher yield in saline fields. This adaptability is associated with the accumulation of reactive oxygen species and activation of the antioxidative defense system, mediated by changes in metabolite concentrations and metabolic homeostasis.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Plant Sciences
Huiju Yang, Mingwei Chen, Jingfeng Hu, Mei Lan, Jiangming He
Summary: In this study, the metabolome and hormone profiles of male sterile and fertile lines of Chinese cabbage were analyzed. The results showed that the male sterile lines had decreased content of flavonoids and phenolamides, while accumulating glucosinolate metabolites. Additionally, the hormone levels in the male sterile lines were significantly lower than those in the fertile lines. This study provides insights into the molecular mechanism of cytoplasmic male sterility in Chinese cabbage.
Article
Agronomy
Naoyuki Katsura, Kanna Itoh, Hiroaki Matsuhira, Yosuke Kuroda, Tomohiko Kubo, Kazuyoshi Kitazaki
Summary: Cytoplasmic male sterility (CMS) is a mitochondrion-encoded trait that specifically affects anther development. This study compared the anther development in two sugar beet CMS lines and found that both lines exhibited degeneration of microspores and tapetum, leading to similar male sterile phenotypes. However, the specific developmental abnormalities differed between the two lines. Additionally, this study revealed that mitochondrial appearance in the root apical meristem was normal, indicating no association between mitochondrial activation and CMS expression.
Article
Biochemistry & Molecular Biology
Haoran Wang, Qing Cheng, Ziqi Zhai, Xiangyun Cui, Mingxuan Li, Ruiquan Ye, Liang Sun, Huolin Shen
Summary: Transcriptomic and proteomic analyses were performed to identify genes and proteins associated with celery cytoplasmic male sterility. The results provide a foundation for further investigation into the mechanisms of pollen development and the causes of cytoplasmic male sterility in celery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Chuande Wang, Lina Lezhneva, Nadege Arnal, Martine Quadrado, Hakim Mireau
Summary: In the study, it was found that PPR-B can revert male sterility in the Ogura CMS system in rapeseed through specific translation inhibition of the mitochondria-encoded CMS causing mRNA orf138. This is the first recognized fertility restorer shown to act in this way.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Yamin Zhang, Qilu Song, Lili Zhang, Zheng Li, Chengshe Wang, Gaisheng Zhang
Summary: This study provides a comprehensive morphological and proteomic analysis of the anthers of a P-type CMS wheat line, revealing potential mechanisms involving disturbed carbohydrate metabolism, inadequate energy supply, and disturbed protein synthesis leading to male sterility. The results contribute valuable insights into the molecular basis of male sterility in wheat, providing a resource for further research in plant biology and sexual reproduction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agronomy
Ayumu Takatsuka, Tomohiko Kazama, Kinya Toriyama
Summary: The study obtained a Tadukan-type CMS line and a restorer line through backcrossing, determined the whole-genome sequences of the TAA mitochondria, and identified the orf312 gene specific to this genome, which showed differential expression patterns at different growth stages.
Article
Horticulture
Aatjima Na Jinda, Maneechat Nikornpun, Nakarin Jeeatid, Siwaporn Thumdee, Kamon Thippachote, Tonapha Pusadee, Jutamas Kumchai
Summary: This study aimed to produce a new maternal line and its maintainer line by transferring genes from another variety to restorer lines, facilitating hybrid seed production. The male sterility gene was successfully identified using molecular marker-assisted selection and pollen viability test. The results confirmed the accuracy of using these markers in the early generation stage of a conventional breeding program.
Article
Plant Sciences
Senlin Xiao, Jingfeng Xing, Tiange Nie, Aiguo Su, Ruyang Zhang, Yanxin Zhao, Wei Song, Jiuran Zhao
Summary: Cytoplasmic male sterility (CMS) is an important trait in hybrid seed production, but the relationship between mitochondrial function and sterility stability is not well-known. This study analyzed two subtypes of CMS-S maize and found that the copy number of mitochondrial gene nad1-exon1 affected sterility stability.
Article
Plant Sciences
Karen C. Chamusco, May N. Milazzo, Kanchan S. Bhan, Terry L. Kamps, Prestina Smith, Modupeoluwa Durojaiye, Cristina D. Moreira, Maria Gallo, Christine D. Chase
Summary: Research found that collapse of CMS-S pollen is a programmed cell death event with morphological features similar to apoptosis regulated by mitochondria in animals. Typically, mitochondrial morphology and activity in CMS-S pollen differ significantly from normal pollen, possibly leading to decreased protection against mitochondrial generation of reactive oxygen species.
Review
Biotechnology & Applied Microbiology
Kinya Toriyama
Summary: Cytoplasmic male sterility (CMS) is a maternally inherited trait caused by interactions between nuclear and mitochondrial genomes. The CMS/Rf system is an excellent model for understanding genetic interactions between mitochondrial and nuclear genomes in plants, and is important for hybrid seed production.
PLANT BIOTECHNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Wenjing Ren, Jinchao Si, Li Chen, Zhiyuan Fang, Mu Zhuang, Honghao Lv, Yong Wang, Jialei Ji, Hailong Yu, Yangyong Zhang
Summary: Hybrid production using Ogura CMS has become an important approach to harness heterosis in vegetables. This article provides an overview of the mapping, cloning, mechanism and application of Ogura CMS and fertility restorer genes in Brassica napus, Brassica rapa, Brassica oleracea and other cruciferous crops, and discusses the current challenges and future research directions in the application of Ogura CMS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Matthieu Simon, Stephanie Durand, Natacha Pluta, Nicolas Gobron, Lucy Botran, Anthony Ricou, Christine Camilleri, Francoise Budar
Article
Multidisciplinary Sciences
Fabrice Roux, Tristan Mary-Huard, Elise Barillot, Estelle Wenes, Lucy Botran, Stephanie Durand, Romain Villoutreix, Marie-Laure Martin-Magniette, Christine Camilleri, Francoise Budar
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Agronomy
Carine Rizzolatti, Paul Bury, Edouard Tatara, Pierre A. Pin, Nathalie Rodde, Helene Berges, Francoise Budar, Hakim Mireau, Jan J. L. Gielen
Article
Plant Sciences
Jose R. Hernandez Mora, Eric Rivals, Hakim Mireau, Francoise Budar
Review
Ecology
R. Dobler, B. Rogell, F. Budar, D. K. Dowling
JOURNAL OF EVOLUTIONARY BIOLOGY
(2014)
Article
Plant Sciences
Pablo Gonzalez-Melendi, Magalie Uyttewaal, Cesar N. Morcillo, Jose Ramon Hernandez Mora, Susana Fajardo, Francoise Budar, M. Mercedes Lucas
JOURNAL OF EXPERIMENTAL BOTANY
(2008)
Article
Biochemistry & Molecular Biology
M. Uyttewaal, N. Arnal, M. Quadrado, A. Martin-Canadell, N. Vrielynck, S. Hiard, H. Gherbi, A. Bendahmane, F. Budar, H. Mireau
Article
Plant Sciences
Michael Moison, Fabrice Roux, Martine Quadrado, Romain Duval, Muriel Ekovich, Duc-Hoa Le, Marie Verzaux, Francoise Budar
Article
Multidisciplinary Sciences
Nicolas Gobron, Cezary Waszczak, Matthieu Simon, Sophie Hiard, Stephane Boivin, Delphine Charif, Aloise Ducamp, Estelle Wenes, Francoise Budar
Article
Plant Sciences
Clement Boussardon, Marie-Laure Martin-Magniette, Beatrice Godin, Abdelilah Benamar, Benjamin Vittrant, Sylvie Citerne, Tristan Mary-Huard, David Macherel, Loic Rajjou, Francoise Budar
FRONTIERS IN PLANT SCIENCE
(2019)
Article
Plant Sciences
Fabien Chardon, Gwendal Cueff, Etienne Delannoy, Fabien Aube, Aurelia Lornac, Magali Bedu, Francoise Gilard, Stephanie Pateyron, Helene Rogniaux, Audrey Gargaros, Hakim Mireau, Loic Rajjou, Marie-Laure Martin-Magniette, Francoise Budar
Article
Plant Sciences
Stephanie Durand, Anthony Ricou, Matthieu Simon, Noemie Dehaene, Francoise Budar, Christine Camilleri
Summary: This study identified three nuclear loci involved in cytoplasmic male sterility in Arabidopsis thaliana, with RFL24 being identified as a key gene encoding a PPR protein related to known Rf PPRs. The research showed that RFL24 promotes pollen abortion, unlike other Rf PPRs, and the sterility caused by the Cvi-0 allele of RFL24 is due to higher expression during early pollen development. Predicted binding sites for RFL24 upstream of two mitochondrial genes suggest its conservation is linked to maintaining mitochondrial function.
Article
Genetics & Heredity
Matthieu Simon, Stephanie Durand, Anthony Ricou, Nathalie Vrielynck, Baptiste Mayjonade, Jerome Gouzy, Roxane Boyer, Fabrice Roux, Christine Camilleri, Francoise Budar
Summary: The study investigated a pollen killer found in hybrids between two accessions of Arabidopsis thaliana. The results revealed that three genetically linked elements are necessary for pollen killer activity, and it operates according to a poison-antidote model. The research also identified a newly evolved gene encoding the antidote, with important structural variations in killer genotypes.
Review
Biochemistry & Molecular Biology
Francoise Budar, Fabrice Roux
PLANT SIGNALING & BEHAVIOR
(2011)