Article
Agriculture, Multidisciplinary
Su-Jin Jun, Jung-A Lee, Young-Wan Kim, Sang-Ho Yoo
Summary: Through site-directed mutagenesis, a robust mutant Bifidobacterium thermophilum (BtAS) strain BtAS(Y414F/P200R) with enhanced turanose productivity was successfully developed, showing an increase in turanose yield by two times compared to the wild type BtAS and completing the reaction in half the time.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Azucena Herrera-Gonzalez, Gema Nunez-Lopez, Nelson Nunez-Dallos, Lorena Amaya-Delgado, Georgina Sandoval, Magali Remaud-Simeon, Sandrine Morel, Javier Arrizon, Lazaro Hernandez
Summary: Enzymatic fructosylation can enhance the water solubility and bioavailability of phlorizin, with levansucrase (Gd_LsdA) being the most efficient enzyme for this purpose.
ENZYME AND MICROBIAL TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yari Cabezas-Perusse, Franck Daligault, Vincent Ferrieres, Olivier Tasseau, Sylvain Tranchimand
Summary: The study successfully increased the transglycosylation yield of beta-d-galactofuranosyl by mutating and screening the catalyst, followed by molecular modeling. The mutations led to significant differences in regioisomer kinetic profiles compared to the wild-type enzyme.
Article
Biotechnology & Applied Microbiology
Zini Wang, Mengying Zhou, Ning Cao, Xiaoli Wang
Summary: This study evaluates the degradation ability of straw lignocellulose-degrading enzymes and constructs a multifunctional lignocellulose-degrading enzyme. Molecular dynamics shows that 3CBH-1 has significantly higher degradation ability than 3CBH-1KS5-4XQD-1B85. The mechanism of enhanced degradability and enzyme stability is explored, and the suitable external environment for straw degradation is determined. This paper provides a feasible scheme for straw biodegradation by obtaining multifunctional and efficient straw lignocellulose-degrading enzymes through protein engineering.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Xia Li, Geng Chen, Qing-Qing Gao, Chun-Fan Xiang, Cheng-Xiao Yuan, Xiao-Ning Li, Yan-Yu Shu, Guang-Hui Zhang, Yan-Li Liang, Sheng-Chao Yang, Chen-Xi Zhai, Yan Zhao
Summary: This study explored the key enzymes involved in cucurbitacins biosynthesis in the Chinese traditional medicinal plant Hemsleya chinensis. Using AlphaFold2 and molecular docking, the researchers successfully predicted the 3D structure of HcOSC6 and its binding features to 2,3-oxidosqualene. Mutagenesis experiments identified three key amino acid residues, E246, M261, and D490, which significantly affected the activity of HcOSC6. This study not only characterized three potential key residues for producing cucurbitacins, but also provided insights into their significant role in metabolic engineering.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Chenze Qi, Liyun Xv, Wenhao Xia, Yunyi Zhu, Yudan Wang, Zhiping Zhang, Haibo Dai, Minmin Miao
Summary: This study identified 12 invertase genes and 12 invertase inhibitor genes from the cucumber genome, and classified them into different types based on their functions. Transcriptome analysis revealed various expression patterns of these genes, including some significantly expressed genes in different tissues, organs, and development stages, and genes potentially involved in biotic and abiotic stress. Further validation through qRT-PCR confirmed the consistent expression patterns of three genes. These findings provide important information for understanding the physiological functions of cucumber invertases and their inhibitors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Cheng Liu, Shuting Hu, Shuyi Liu, Weiling Shi, Debin Xie, Qi Chen, Hui Sun, Linjing Song, Ziyu Li, Rui Jiang, Dianqiu Lv, Jichun Wang, Xun Liu
Summary: Cell wall invertase (CWI) is an essential coordinator in plant development and carbohydrate partitioning. The regulation of CWI activity depends on post-translational mechanisms by inhibitors (INHs). In this study, StInvInh1 was identified as a CWI inhibitor in potato and was found to regulate microtuber development through fine-tuning sucrose metabolism.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Sachin Kumar, Vijay Kumar Bhardwaj, Anish Kaachra, Shweta Guleria, Arun Kumar, Rituraj Purohit, Sanjay Kumar
Summary: The engineered Cu, Zn SOD (WT) with a substitution of a conserved residue at position 61 (P61G) showed higher catalytic efficiency and thermostability compared to the wild-type protein. The P61G mutation also resulted in enhanced tolerance to hydrogen peroxide inactivation, making it a potential candidate for applications in the food and pharmaceutical industries.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Waraporn Auiewiriyanukul, Wataru Saburi, Tomoya Ota, Jian Yu, Koji Kato, Min Yao, Haruhide Mori
Summary: The study found that the Asn258 mutants of BspAG13_31A have significant impacts on the structure and function of the enzyme. N258G/P mutations enhance trisaccharide specificity, the N258P mutation also enhances activity towards sucrose and produces erlose through transglucosylation. N258G shows a higher specificity for transglucosylation with p-nitrophenyl alpha-D-glucopyranoside and maltose.
Article
Biotechnology & Applied Microbiology
Suling Zhang, Zhen Zhang, Xun Sun, Zhiqiang Liu, Min Ma, Jinbu Fan, Weiqi Luo, Libin Wang, Shaoling Zhang
Summary: The 23 invertase genes identified in pear genome, including different types such as vacuolar, cell wall, and alkaline/neutral invertases, exhibit tissue-specific expression profiles and can be affected by postharvest treatments. Changes in invertase activity and gene expression profiles during pear development may be associated with visual/inner quality alterations, with PbrvacInv1 potentially playing a role in sucrose decomposition.
Article
Biochemistry & Molecular Biology
Huanyu Wang, Qianru Lin, Mingwang Liu, Wen Ding, Nanhai Weng, Hao Ni, Jing Lu, Mingsheng Lyu, Shujun Wang
Summary: In this study, we used molecular docking technique to study the potential effect of key amino acids in the engineered strain expressed dextranase on its enzymatic properties. The results showed that mutant enzyme H373A significantly increased the yield of IMO4 to 62.32%. Saturation mutation of H373R and its neighboring site S374R also increased the yield of IMO4. Analysis of the enzymatic properties revealed that H373R had a lower optimum temperature and maintained high enzyme activity under alkaline conditions. The findings provide important theoretical basis for the future production of IMO4 and analysis of dextranase structure.
Article
Biotechnology & Applied Microbiology
Xu Chen, Zhidong Chen, Daiyun Xu, Yonghui Lyu, Yongxiao Li, Shengbin Li, Junqing Wang, Zhe Wang
Summary: Machine learning model combined with SDM successfully generated and optimized potential peptide agonists targeting GPR40, showing comparable activity with the model agonist in phase III clinical trials. The combination of MFS, DNN, and CNN accurately predicted the activity of peptides, with R-square reaching 0.87 and 0.75 for the training and test sets respectively. MD simulations confirmed the stability of peptide-protein complexes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Yemin Xue, Mengke Xue, Fang Xie, Mengchen Zhang, Hongyang Zhao, Tao Zhou
Summary: Alkyl glycosides, known nonionic surfactants, can be synthesized via transglycosylation reactions with GH1 glycosidases, but the yields may be limited by hydrolysis. Mutants of a beta-glucosidase, including N222F, N223C, N223Q, G224A, Y295F, and F414S, were produced to improve transglycosylation while reducing hydrolysis, resulting in altered substrate specificity and increased yields of hexyl-beta-glycoside.
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Microbiology
Emily C. Hoedt, Francesca Bottacini, Nora Cash, Roger S. Bongers, Kees van Limpt, Kaouther Ben Amor, Jan Knol, John MacSharry, Douwe van Sinderen
Summary: Members of the genus Bifidobacterium are difficult to genetically manipulate due to their extensive and variable Restriction-Modification systems. A modified suicide vector, pFREM28, was developed to target specific genes in B. breve by removing known restriction sites, reducing the time, effort, and resources required for generating site-directed mutants. This approach could be applied to other (bifido)bacterial species as well.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Zhanqing Fan, Li Fang, Limei Wu, Zheren Wang, Yanan Wang, Caijing Han, Xiaoting Liu
Summary: This study aimed to improve the catalytic activity of aspartate kinase (AK), the first key enzyme in the aspartic acid metabolism pathway, and weaken the feedback inhibition of metabolites. The mutant A380M with significantly increased enzyme activity showed enhanced substrate affinity. Molecular dynamics simulations revealed changes in the binding force and interaction between AK and its substrates.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2022)
Article
Chemistry, Applied
Maira Jimenez-Sanchez, Rebeca Perez-Morales, Francisco M. Goycoolea, Monika Mueller, Werner Praznik, Renate Loeppert, Victor Bermudez-Morales, Guadalupe Zavala-Padilla, Marcela Ayala, Clarita Olvera
CARBOHYDRATE POLYMERS
(2019)
Article
Biochemistry & Molecular Biology
Liya Muslinkina, Abigail Roldan-Salgado, Paul Gaytan, Victor R. Juarez-Gonzalez, Enrique Rudino, Nadya Pletneva, Vladimir Pletnev, Zbigniew Dauter, Sergei Pletnev
JOURNAL OF MOLECULAR BIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Victor Cifuentes-Castro, Claudia Rodriguez-Almazan, Jesus Silva-Sanchez, Enrique Rudino-Pinera
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Soil Science
Sarah Symanczik, Jennifer Kruetzmann, Uwe Nehls, Thomas Boller, Pierre-Emmanuel Courty
SOIL BIOLOGY & BIOCHEMISTRY
(2020)
Article
Plant Sciences
Silvia Calabrese, Loic Cusant, Alexis Sarazin, Annette Niehl, Alexander Erban, Daphnee Brule, Ghislaine Recorbet, Daniel Wipf, Christophe Roux, Joachim Kopka, Thomas Boller, Pierre-Emmanuel Courty
FRONTIERS IN PLANT SCIENCE
(2019)
Article
Biochemistry & Molecular Biology
Enrique Raga-Carbajal, Adelaida Diaz-Vilchis, Sonia P. Rojas-Trejo, Enrique Rudino-Pinera, Clarita Olvera
Summary: This study clarified the interaction of the enzyme with an acceptor/product oligosaccharide and the molecular basis of the nonprocessive levan elongation mechanism of levansucrases. Mutations affecting residues involved in substrate binding showed altered substrate affinity and turnover numbers, while an additional oligosaccharide-binding site potentially participates in the elongation mechanism.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Microbiology
Anaid Silva-Salinas, Melissa Rodriguez-Delgado, Jesus Gomez-Trevino, Ulrico Lopez-Chuken, Clarita Olvera-Carranza, Edgar Allan Blanco-Gamez
Summary: This study analyzed the thermosability and effect of calcium ions on the enzymatic activity of alpha-amylase produced by Bacillus licheniformis strain LB04 isolated from Espinazo Hot Springs in Nuevo Leon, Mexico. The immobilized agar-agarose alpha-amylase showed approximately 25% higher hydrolytic activity compared to the free enzyme.
Article
Mycology
Aime Frida Moran-Mejia, Rosa Angelica Guillen-Garces, Gabriela Eleonora Moeller-Chavez, Jesus Hernandez-Romano, Maria Ines Chavez-Bejar, Clarita Olvera-Carranza, Luis Gerardo Trevino-Quintanilla
Summary: Isolating and identifying oleaginous yeast strains capable of producing lipids from centrifuged nejayote, hydrolyzed nejayote solids, and hydrolyzed sugarcane bagasse demonstrated the potential for utilizing these agricultural and industrial residues for biotechnological purposes.
REVISTA IBEROAMERICANA DE MICOLOGIA
(2022)
Article
Biochemistry & Molecular Biology
Yerli Marin-Tovar, Hugo Serrano-Posada, Adelaida Diaz-Vilchis, Enrique Rudino-Pinera
Summary: PCNA is an essential protein for cell viability in archaea and eukarya, involved in DNA replication and repair. This study reveals that PCNA has the intrinsic ability to resist high levels of ionizing radiation, with a specific composition of amino acid residues and their synergistic interactions.
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2022)
Article
Microbiology
Flor de Maria Garcia-Paz, Salvador Martinez-Bahena, Clarita Olvera
Summary: Levansucrase LevS from Leuconostoc mesenteroides B-512F is a multidomain fructansucrase that contains additional domains (ADs) which play essential roles in stability, activity, specificity, and polymerization processes. The N-terminal region and the transition region of LevS are crucial for these processes. This study provides insights into the structural-functional relationship of Levansucrase from Leuconostocaceae family.
Article
Obstetrics & Gynecology
Geny Del Socorro Fierros-Zarate, Fernando Luis Reyna-Flores, Ana Isabel Burguete, Vicente Madrid-Marina, Eduardo Guzman-Olea, Clarita Olvera Carranza, Celina Garcia Melendrez, Victor Hugo Bermudez-Morales
Summary: The study aimed to investigate the effect of aspirin on epithelial HPV16-transformed cells and its anti-tumor effects in an experimental HPV 16 positive tumor model. The study design was experimental (in vitro and in vivo). SiHa and BMK-16/myc cells were treated with aspirin and cell proliferation was measured. Caspase-Glo 3/7 Assay was used to determine apoptosis. Tumor-bearing mice were orally treated with 50 mg/gr/day of aspirin for 30 days and the antitumor effect was evaluated.
GYNECOLOGIC AND OBSTETRIC INVESTIGATION
(2023)
Article
Biochemistry & Molecular Biology
Alejandra Melendez-Zempoalteca, Victor Rivelino Juarez-Gonzalez, Enrique Rudino-Pinera, Nina Pastor, Leonel Vargas-Jaimes, Jose Antonio Valcarcel-Gamino, Oscar Fernando Vazquez-Vuelvas, Veronica Quintero-Hernandez, Laura Leticia Valdez-Velazquez
Summary: Scorpionism in Mexico caused by Centruroides scorpions is a public health problem. This study describes the production of recombinant toxins from the venom of Centruroides tecomanus scorpion and the development of polyclonal antibodies that neutralize the venom. The results suggest the potential for producing a region-specific antivenom.
INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
(2022)
Article
Biochemistry & Molecular Biology
Enrique Rudino-Pinera, Angel E. Pelaez-Aguilar, Carlos Amero, Adelaida Diaz-Vilchis
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2019)
Meeting Abstract
Biochemistry & Molecular Biology
A. Niehl, I. Wyrsch, T. Boller, M. Heinlein
MOLECULAR PLANT-MICROBE INTERACTIONS
(2019)