Article
Multidisciplinary Sciences
Marcos Penedo, Keisuke Miyazawa, Naoko Okano, Hirotoshi Furusho, Takehiko Ichikawa, Mohammad Shahidul Alam, Kazuki Miyata, Chikashi Nakamura, Takeshi Fukuma
Summary: The nanoendoscopy-AFM technique allows for imaging of actin fiber 3D maps and membrane inner scaffold 2D nanodynamics inside living cells without affecting cell viability. This overcomes the limitations of current nanoimaging techniques by directly accessing intracellular components with AFM capabilities.
Review
Biochemistry & Molecular Biology
Leia Colin, Raquel Martin-Arevalillo, Simone Bovio, Amelie Bauer, Teva Vernoux, Marie-Cecile Caillaud, Benoit Landrein, Yvon Jaillais
Summary: Biological imaging is an interdisciplinary field crucial in cell biology research, involving techniques, probe development, and image analysis. Recent advances in live imaging of plant cells include studies on cell structure, hormones, mechanical properties, etc. driving the field forward.
Article
Multidisciplinary Sciences
Ka Man Carmen Chan, Ashley L. Arthur, Johannes Morstein, Meiyan Jin, Abrar Bhat, Dorte Schlesinger, Sungmin Son, Donte A. Stevens, David G. Drubin, Daniel A. Fletcher
Summary: Fusion-associated small transmembrane (FAST) proteins are a diverse family of nonstructural viral proteins that drive cell-cell fusion, increasing viral spread and pathogenicity. Unlike viral fusogens with tall ectodomains, FAST proteins have short fusogenic ectodomains that cannot bridge the intermembrane gap between neighboring cells. This work suggests that localized mechanical pressure on the plasma membrane coupled to a membrane-disruptive ectodomain is sufficient to drive cell-cell fusion.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Multidisciplinary
Saori Miho, Keiichi Imato, Yousuke Ooyama
Summary: In this study, a PET-type fluorescent monomer SM-2 and its copolymer poly(SM-2-co-MMA) were designed and developed for visualization of a trace amount of water in solvents. Both SM-2 and poly(SM-2-co-MMA) exhibited fluorescence enhancement with increasing water content in various solvents and can act as fluorescent sensors.
Review
Plant Sciences
Sunil Kumar, Theboral Jeevaraj, Mohd H. Yunus, Subhra Chakraborty, Niranjan Chakraborty
Summary: This review summarizes recent advances in the role of plant cytoskeleton and associated proteins in abiotic stress management. Plants have evolved specific mechanisms to sense external stress and transmit information for appropriate responses. The plant cytoskeleton, along with associated proteins, not only plays a role in cell growth and proliferation but also functions in stress response and resilience.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Developmental Biology
Zengyu Liu, Isabella Osterlund, Felix Ruhnow, Yiran Cao, Guoqiang Huang, Wenguo Cai, Jiao Zhang, Wanqi Liang, Zoran Nikoloski, Staffan Persson, Dabing Zhang
Summary: Rice is an important food crop, and the cytoskeleton plays a crucial role in its growth, development, and response to the environment. However, reliable markers for the cytoskeleton in rice have been lacking, limiting our understanding of its functions. In this study, fluorescent marker lines for the actin and microtubule cytoskeletons in rice were generated, and their roles were investigated in pollen grain development, root cell development, and salt stress response.
Article
Engineering, Environmental
Jinzhu Gao, Rongchen Wang, Kai Yin, Tianli Zhu, Jie Sun, Chengjun Dong, Xuemei Dong, Mengting Zhao, Xianfeng Gu, Chunchang Zhao
Summary: Visualizing the complex crosstalk between biological signaling molecules, such as H2O2 and H2S, is urgent. In this study, dynamic-reversible probes with dually responsive sites were engineered to visualize the fluctuations of H2O2 and H2S. The designed reporter features self-immolation and aromatic nucleophilic reaction to yield sulfhydryl-functionalized intermediate probe for bright NIR emissions. The probe can repeatedly cycle S-oxidation and reduction processes to alternate fluorescence, visualizing the intracellular coexistence of H2O2 and H2S.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Nori Nakai, Keisuke Sato, Tomomi Tani, Masahiko Kawagishi, Hiromasa Ka, Kenta Saito, Sumio Terada
Summary: POLArIS is a versatile probe for monitoring the dipole orientation of target molecules using fluorescence polarization microscopy. New versions of POLArIS with nanobodies and extended color variations enable multi-color orientation imaging for multiple targets, enhancing the probe's performance for broader applications in living cells, tissues, and whole organisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Zhiming Ma, Kexin Zhu, Yong-Gui Gao, Suet-Mien Tan, Yansong Miao
Summary: The actin cytoskeleton undergoes rapid remodelling during signal transduction, and the plant class I formin family plays a major role in this process. The PM-integrated formins are highly responsive to mechanical perturbation, regulating their condensation on the cell surface. However, the molecular mechanisms underlying the mechanosensing and mechanoregulation of formin in actin remodelling remain unclear.
Article
Chemistry, Multidisciplinary
Ya-Xi Ye, Ya-Wen Yu, Zhu-Min Xu, Xin-Yue Chen, Zhong-Chang Wang, Bao-Zhong Wang, Qing-Cai Jiao, Hai-Liang Zhu
Summary: A novel fluorescent probe, PE-YW, was designed for selectively detecting Cys by regulating its AIE-based effects. The probe utilized a specific conjugate addition cyclization reaction between Cys and an acrylate moiety to induce fluorescence turn-on. PE-YW showed high selectivity, low detection limit, and excellent performance for detecting Cys in living systems.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Spectroscopy
Zhengbing Zhou, Xiao Yuan, Da Long, Manhua Liu, Kun Li, Yongmei Xie
Summary: A lysosomal-targeted near infrared fluorescent probe with high sensitivity towards reactive oxygen species (ROS) was developed, showing different response activity towards H2O2, · OH, and HClO. It has been successfully used for ROS detection and imaging in cells and in vivo.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Yali Cui, Chenggong Xu, Tian Wu, Yamin Nie, Yanmei Zhou
Summary: Researchers successfully visualized the in situ release of hydrazine in living systems using the designed cyanine-based fluorescent probe B-CyAS, providing important insights into drug metabolism in vivo. Their study offers a promising tool and method for monitoring and evaluating toxic metabolites in biological systems.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Fangfang Meng, Tiantian Liu, Qing Lv, Junyi He, Jie Niu, Peng Gao, Xiaoqiang Yu
Summary: Mitochondrial fragmentation is a crucial physiological process associated with energy production, metabolism, and apoptosis. However, the details of morphological changes during this process are rarely reported. In this study, a leakage-free fluorescent probe called IP-8 was developed and successfully used to visualize and track the details of mitochondrial fragmentation process in real-time and in situ. IP-8 was suggested as a useful tool for studying mitochondrial morphology changes.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Cell Biology
Ingrid Billault-Chaumartin, Laetitia Michon, Caitlin A. Anderson, Sarah E. Yde, Cristian Suarez, Justyna Iwaszkiewicz, Vincent Zoete, David R. Kovar, Sophie G. Martin
Summary: In formin-family proteins, the nucleation and elongation activities of actin filaments are located in the formin homology 1 (FH1) and FH2 domains, with varying reaction rates among different formins. This study investigates the importance of nucleation and elongation rates of formins in vivo using the formin Fus1 in Schizosaccharomyces pombe. It is found that changes in the nucleation and elongation rates have direct effects on the architecture of the actin fusion focus, and that Fus1's native high nucleation and low elongation rates are optimal for assembly of the fusion focus. A point mutant in the FH2 domain of Fus1 is also identified, which preserves the native nucleation and elongation rates in vitro but alters the function in vivo, indicating additional properties of the FH2 domain. Thus, the rates of actin assembly are tailored for assembly of specific actin structures.
JOURNAL OF CELL SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Xu Tong, Liguo Hao, Xue Song, Shuang Wu, Na Zhang, Zhongtao Li, Song Chen, Peng Hou
Summary: In this study, a new fluorescent probe COZ-DNBS was developed for the rapid and sensitive detection of H2S. The probe exhibited excellent selectivity and showed promise for monitoring H2S levels in realistic samples and living organisms.
