Article
Plant Sciences
Ru-Jie Li, Chun-Xiao Zhang, Sheng-Yao Fan, Yi-Han Wang, Jiangqi Wen, Kirankumar S. Mysore, Zhi-Ping Xie, Christian Staehelin
Summary: This study reveals the presence of a second Nod factor cleaving hydrolase (MtCHIT5b) in Medicago truncatula, which degrades Sinorhizobium meliloti Nod factors in the rhizosphere in conjunction with MtNFH1. The expression of MtCHIT5b is induced by purified Nod factors treatment or rhizobia inoculation, and it is localized in the infection pocket of root hairs. The study also demonstrates that overexpression of MtCHIT5b leads to reduced nodule formation, and simultaneous silencing of MtCHIT5b and MtNFH1 results in decreased nodulation.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Marie-Laure Martin, Marjorie Pervent, Ilana Lambert, Stefano Colella, Mathilde Tancelin, Dany Severac, Gilles Clement, Pascal Tillard, Florian Frugier, Marc Lepetit
Summary: In mature symbiotic root nodules, rhizobia fix atmospheric dinitrogen to provide ammonium for plant nitrogen demand. The symbiosis is adjusted to the plant's nitrogen demand through systemic N signaling, controlling nodule development. Nodule expansion is stimulated under N deficit, while nodule senescence is activated under N satiety. Drought has a negative impact on nodules. In this study, split-root systems were used to characterize the systemic responses of symbiotic plants to localized osmotic stress. The application of PEG inhibited the symbiotic dinitrogen fixation activity of locally treated nodules, leading to nitrogen limitation. The localized PEG treatment triggered systemic signaling, stimulating nodule development and enhancing sucrose allocation. Transcriptomic reprogramming associated with PEG and N deficit signaling shared many target transcripts. These findings suggest that systemic N signaling plays a role in the adaptation of symbiotic plants to local variations in their edaphic environment.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Liangliang Yu, Qi Di, Danping Zhang, Yumin Liu, Xiaolin Li, Kirankumar S. Mysore, Jiangqi Wen, Junhui Yan, Li Luo
Summary: PSK-delta, a novel legume-specific phytosulfokine, promotes symbiotic nodulation by enhancing nodule organogenesis.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Thi-Bich Luu, Anna Ourth, Cecile Pouzet, Nicolas Pauly, Julie Cullimore
Summary: A newly evolved gene LYK2bis in Medicago truncatula extends nodulation specificity and is important for nodulation by mutants producing nonO-acetylated NFs and by many natural Sinorhizobia strains.
Review
Plant Sciences
Justin P. Hawkins, Ivan J. Oresnik
Summary: The interaction between bacteria and plants can be positive, negative, or neutral. The rhizobium-legume interaction is a well-studied positive interaction model. This review discusses how rhizobia tolerate stresses and how these stress tolerance mechanisms play a role in signal exchange between rhizobia and legumes.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Ginaini Grazielli Doin de Moura, Saida Mouffok, Nil Gaudu, Anne-Claire Cazale, Marine Milhes, Tabatha Bulach, Sophie Valiere, David Roche, Jean-Baptiste Ferdy, Catherine Masson-Boivin, Delphine Capela, Philippe Remigi
Summary: During the experimental evolution of a plant pathogenic bacterium into a legume symbiont, improved competitiveness for host entry was the main driver of fast adaptation, outweighing adaptation to within-host proliferation. Continuous accumulation of new mutations and sequential sweeps of mutations were observed, with multiple adaptive mutations co-occurring in the same cohort. Selective bottlenecks before within-host proliferation and after the hypermutagenesis phase were found to alter the relative influence of selective pressures during bacterial adaptation to multistep infection processes.
MOLECULAR BIOLOGY AND EVOLUTION
(2023)
Article
Agronomy
Chao Ma, Shengnan Ma, Qinglin Kang, Yue Wang, Yutian Sun, Zhaoming Qi, Jianan Zou, Chunyan Liu, Mingliang Yang, Dawei Xin, Qingshan Chen, Jinhui Wang
Summary: Soybean and rhizobium symbiosis is influenced by Nod factor signaling pathway, particularly the NodB gene. Using soybean recombinant inbred lines, genes related to nodule number and weight were identified.
Article
Plant Sciences
Thomas B. Irving, Sanhita Chakraborty, Sergey Ivanov, Michael Schultze, Kirankumar S. Mysore, Maria J. Harrison, Jean-Michel Ane
Summary: This study characterized the symbiotic phenotypes of four Medicago truncatula mutants and identified two genes, RAM1 and KIN3, involved in mycorrhization. The results showed that RAM1 acts upstream of KIN3 and that KIN3 is involved in suppressing plant defenses and promoting root colonization. KIN3 also plays an essential role in the symbiotic response to soil nitrogen levels.
Article
Plant Sciences
Paolo M. Triozzi, Thomas B. Irving, Henry W. Schmidt, Zachary P. Keyser, Sanhita Chakraborty, Kelly Balmant, Wendell J. Pereira, Christopher Dervinis, Kirankumar S. Mysore, Jiangqi Wen, Jean-Michel Ane, Matias Kirst, Daniel Conde
Summary: Most legumes can establish a symbiotic relationship with soil rhizobia, promoting the formation of root nodules for efficient nitrogen fixation. Coordination of nodule organogenesis and rhizobial infection in space and time is crucial for successful nodulation. The plant hormone cytokinin (CK) plays a key role as a positive regulator in nodule organogenesis, but the temporal regulation of tissue-specific CK signaling and biosynthesis in response to bacterial lipo-chitooligosaccharides (LCOs) or Sinorhizobium meliloti inoculation remains poorly understood.
Article
Biochemistry & Molecular Biology
Thi-Bich Luu, Noemie Carlos, Louis Bouzou, Chrystel Gibelin-Viala, Celine Rembliere, Virginie Gasciolli, Jean-Jacques Bono, Benoit Lefebvre, Nicolas Pauly, Julie Cullimore
Summary: This study reveals the function of the LYK gene cluster in strain-specific recognition in the Medicago truncatula genotypes A17 and R108, as well as the ability of their encoded proteins to bind NFs. The essential role of LYK3 in nodulation is not conserved in R108 despite similar sequences and good nodulation expression profiles. Although LYK2, LYK5, and LYK5bis are not essential for nodulation, they may play accessory roles in nodulation through low-affinity NF binding.
Article
Plant Sciences
Li Wang, Jieyu Yang, Wenjun Tan, Yile Guo, Jiaqi Li, Chuntao Duan, Gehong Wei, Minxia Chou
Summary: In the symbiosis of M. truncatula-Sinorhizobium meliloti, MtMIF3 is mainly expressed in the nitrogen-fixing zone of the nodules. Silencing MtMIF3 increased nodule numbers but resulted in higher bacteroid degradation in the infected cells of the nitrogen-fixing zone, indicating premature aging. On the other hand, overexpression of MtMIF3 inhibited nodule senescence.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Szilard Kovacs, Erno Kiss, Sandor Jenei, Erzsebet Feher-Juhasz, Attila Kereszt, Gabriella Endre
Summary: Legumes are able to meet their nitrogen need by establishing nitrogen-fixing symbiosis with rhizobia. A novel Medicago truncatula early symbiotic mutant identified as infection-related epidermal factor (ief) was described, in which the formation of infection threads (ITs) is blocked in the root hair cells and only nodule primordia are formed. MtIEF is crucial for bacterial infection in the root epidermis but not required for nodule organogenesis.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2022)
Article
Biochemistry & Molecular Biology
Sergio Alan Cervantes-Perez, Sandra Thibivilliers, Carole Laffont, Andrew D. Farmer, Florian Frugier, Marc Libault
Summary: This study used single nuclei RNA-seq to analyze the early response to rhizobial infection in Medicago roots. A gene expression map of the Medicago root was generated, revealing known and novel genes and pathways involved in the symbiotic relationship between legumes and rhizobia. This comprehensive analysis provides insights into cell type-specific responses during root symbiosis.
Article
Plant Sciences
Marie-Francoise Jardinaud, Sebastien Carrere, Benjamin Gourion, Pascal Gamas
Summary: Symbiotic nitrogen fixation plays a key role in reducing the negative impact of nitrogen fertilizers in agroecosystems. The efficiency of this process is influenced by the combination of bacterial and plant genotypes, but the mechanisms behind differences in the efficiency of rhizobium strains are not well known.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Plant Sciences
Feixue Liao, Grmay Hailu Lilay, Pedro Humberto Castro, Herlander Azevedo, Ana G. L. Assuncao
Summary: This study investigates the regulatory mechanism and evolutionary conservation of zinc deficiency response in legume plants, focusing on the F-bZIP transcription factors. The results provide insights into the functional roles of F-bZIPs and related genes in legume crops, contributing to the development of strategies for improving zinc content.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biology
Julien S. Luneau, Mael Baudin, Thomas Quiroz Monnens, Sebastien Carrere, Olivier Bouchez, Marie-Francoise Jardinaud, Carine Gris, Jonas Francois, Jayashree Ray, Babil Torralba, Matthieu Arlat, Jennifer D. Lewis, Emmanuelle Lauber, Adam M. Deutschbauer, Laurent D. Noel, Alice Boulanger
Summary: Plant diseases pose a significant threat to food production, but our understanding of pathogen survival mechanisms during host colonization is limited. By conducting a genome-wide screen, we identified key genes involved in the adaptation and fitness of the Xcc pathogen in the cauliflower host plant. The study provides insights into the social behaviors and regulatory mechanisms of Xcc during early infection stages.
Article
Plant Sciences
Laurent Sauviac, Antoine Remy, Emeline Huault, Melanie Dalmasso, Theophile Kazmierczak, Marie-Francoise Jardinaud, Ludovic Legrand, Corentin Moreau, Bryan Ruiz, Anne-Claire Cazale, Sophie Valiere, Benjamin Gourion, Laurence Dupont, Veronique Gruber, Eric Boncompagni, Eliane Meilhoc, Pierre Frendo, Florian Frugier, Claude Bruand
Summary: This study identified hundreds of plant and bacterial genes associated with nodule senescence through dual plant-bacteria RNA sequencing, providing a valuable resource for exploring the mechanisms of nodule senescence. The study revealed that genes related to both plant and bacterial cell cycle and stress responses play important roles in nodule senescence, suggesting complex regulatory networks involved in this process.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Plant Sciences
Marie-Francoise Jardinaud, Sebastien Carrere, Benjamin Gourion, Pascal Gamas
Summary: Symbiotic nitrogen fixation plays a key role in reducing the negative impact of nitrogen fertilizers in agroecosystems. The efficiency of this process is influenced by the combination of bacterial and plant genotypes, but the mechanisms behind differences in the efficiency of rhizobium strains are not well known.
PLANT AND CELL PHYSIOLOGY
(2023)
Review
Plant Sciences
Johan Rodriguez Melo, Florencia Mammarella, Federico Ariel
Summary: The increasing world population and global warming have significantly impacted agriculture, leading to the emergence of new pests and pathogens that threaten crop productivity. To address this, the scientific community is urgently developing innovative solutions, such as using RNA-based technologies as an alternative to synthetic pesticides. The stabilization and delivery of RNA through nanotechnology is crucial for its versatile applications in sustainable agriculture. This eco-friendly approach can also help plants adapt to extreme environmental conditions in the context of climate change.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Romina B. Agostini, Federico Ariel, Sebastian P. Rius, Walter A. Vargas, Valeria A. Campos-Bermudez
Summary: Inoculation of maize roots with Trichoderma leads to epigenetic marks in genes related to plant defense pathways, resulting in enhanced immunity against Colletotrichum infection. The activation of plant responses is mediated by priming mechanisms, involving changes in phytohormone levels and chromatin structure. This study provides insights into the interplay between phytohormones and epigenetic regulation in the long-lasting defense response of maize plants to Trichoderma inoculation.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Review
Plant Sciences
Jun Yang, Federico Ariel, Dong Wang
Summary: This review integrates recent advances in plant long non-coding RNA regulatory mechanisms and provides an outlook for their potential application in plant breeding.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Natanael Mansilla, Camille Fonouni-Farde, Federico Ariel, Leandro Lucero
Summary: The study focused on the TB1 clade of the TCP transcription factor family in grasses and identified an amino acid replacement within the TIG1 clade that affects chromatin binding of TFs and impacts downstream gene expression and plant development.
Review
Biochemistry & Molecular Biology
Pablo A. Manavella, Micaela A. Godoy Herz, Alberto R. Kornblihtt, Reed Sorenson, Leslie E. Sieburth, Kentaro Nakaminami, Motoaki Seki, Yiliang Ding, Qianwen Sun, Hunseung Kang, Federico D. Ariel, Martin Crespi, Axel J. Giudicatti, Qiang Cai, Hailing Jin, Xiaoqi Feng, Yijun Qi, Craig S. Pikaard
Summary: This article presents and discusses new and long-standing open questions in plant RNA biology, covering topics such as alternative splicing, dynamics, translation, structures, epitranscriptomics, long non-coding RNAs, small RNA production and functions, and more. It provides insights into the current state-of-the-art in the field and aims to provoke future discoveries and reflections.
Letter
Plant Sciences
Victoria Gastaldi, Antonela L. Alem, Natanael Mansilla, Federico D. Ariel, Ivana L. Viola, Leandro E. Lucero, Daniel H. Gonzalez
Summary: The Arabidopsis homeodomain protein BREVIPEDICELLUS/KNAT1 functions by repressing the expression of the gene encoding the transcription factor TCP15, thereby limiting filament growth during late stages of stamen development.
Article
Plant Sciences
Nathan Diplock, Mael Baudin, Leslie Harden, Christopher J. Silva, Melissa L. Erickson-Beltran, Jana A. Hassan, Jennifer D. Lewis
Summary: The highly conserved angiosperm immune receptor ZAR1 recognizes diverse pathogen effector proteins by monitoring the ZRK family. The study found that ZAR1 can interact with most ZRKs, except ZRK7, in Arabidopsis thaliana. Alternative splicing of ZRK7 generates a protein that can still interact with ZAR1. The findings suggest a greater diversity of ZAR1 interactions with kinases and the potential for expanding ZAR1 immunodiversity.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Plant Sciences
Tom Laloum, Sofia D. Carvalho, Guiomar Martin, Dale N. Richardson, Tiago M. D. Cruz, Raquel F. Carvalho, Kevin L. Stecca, Anthony J. Kinney, Mathias Zeidler, Ines C. R. Barbosa, Paula Duque
Summary: The plant-specific SCL30a SR protein negatively regulates ABA signaling to control seed traits and stress responses during germination in Arabidopsis. Loss of SCL30a function induces ABA-responsive gene expression and genes repressed during germination, resulting in delayed germination and hypersensitivity to ABA and high salinity in scl30a mutant seeds. Transgenic plants overexpressing SCL30a exhibit reduced ABA and salt stress sensitivity.
PLANT CELL AND ENVIRONMENT
(2023)
Review
Horticulture
Natanael Mansilla, Lucia Ferrero, Federico D. Ariel, Leandro E. Lucero
Summary: LHP1 controls gene expression in plants by regulating the spreading of H3K27me3 mark, and it plays crucial roles in the development and physiology of horticultural crops such as melon, tomato, and soybean.
Article
Horticulture
Johan Rodriguez Melo, Maria Laura Tonelli, Maria Carolina Barbosa, Federico Ariel, Zifan Zhao, Jianping Wang, Adriana Fabra, Fernando Ibanez
Summary: In this study, members of the LysM-RLK family in the peanut genome were identified and the evolutionary history of the family was reconstructed. These proteins are involved in symbiosis development and defense responses, and may play a role in the receptor network mediating the perception of Nod factors and chitosan.
Article
Plant Sciences
Marie-Francoise Jardinaud, Justine Fromentin, Marie-Christine Auriac, Sandra Moreau, Yann Pecrix, Ludivine Taconnat, Ludovic Cottret, Gregoire Aubert, Sandrine Balzergue, Judith Burstin, Sebastien Carrere, Pascal Gamas
Summary: The study reveals that nitrogen-fixing symbiosis recruited two homologous root transcription factors for nodule development, providing a key insight into the coordination of plant and bacterial cell differentiation during this process.