Article
Multidisciplinary Sciences
Zhansheng Li, Yumei Liu, Suxia Yuan, Fengqing Han, Zhiyuan Fang, Limei Yang, Mu Zhuang, Yangyong Zhang, Honghao Lv, Yong Wang, Jialei Ji
Summary: In this study, the fine mapping of sulforaphane metabolism quantitative trait loci (QTLs) in broccoli florets was conducted, revealing a total of 18 QTLs with 6 QTLs being stably detected in multiple environments, providing important data support for future marker-assisted selection breeding.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Yaobin Liu, Rukmini Mukherjee, Florian Bonn, Thomas Colby, Ivan Matic, Marius Glogger, Mike Heilemann, Ivan Dikic
Summary: The SidE family of Legionella effectors, particularly SdeA, catalyzes non-canonical phosphoribosyl-linked ubiquitination of host proteins to disrupt Golgi integrity. This disruption prevents Golgi linking proteins GRASP55 and GRASP65 from clustering and forming oligomeric structures on multiple serine residues, impacting the host secretory pathway. The Golgi manipulation strategy by Legionella highlights how the bacterium hijacks the secretory pathway to promote bacterial infection.
CELL DEATH AND DIFFERENTIATION
(2021)
Editorial Material
Cell Biology
Jeffrey Knupp, Yu-Jie Chen, Anoop Arunagiri, Leena Haataja, Peter Arvan, Billy Tsai
Summary: The study reveals that RTN3 recruits misfolded prohormones for lysosomal degradation through PGRMC1, selectively targeting small oligomers rather than large protein aggregates. This finding suggests that PGRMC1 could be a potential intervention point for diseases caused by ER protein retention.
Article
Biochemistry & Molecular Biology
Leena Haataja, Anoop Arunagiri, Anis Hassan, Kaitlin Regan, Billy Tsai, Balamurugan Dhayalan, Michael A. Weiss, Ming Liu, Peter Arvan
Summary: Efficient export of proinsulin from the endoplasmic reticulum requires the formation of specific disulfide bonds, while disrupting certain pairings may improve trafficking in some mutants. Mutations causing Mutant INS gene-induced Diabetes of Youth (MIDY) often result in perturbed proinsulin disulfide bond formation, with potential for allele-specific pharmacological targeting in the future.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Microbiology
Anthony V. Morante, Daniel Davidnan Baboolal, Xavier Simon, Elizabeth, Mark Cheng-Ying Pan, Patricio I. Meneses
Summary: The amino acid residues 43-DQILQ-47 of the HPV16 L2 protein play a crucial role in the intracellular trafficking of the virus, particularly in trafficking beyond the endoplasmic reticulum.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Fuzhen Sun, Runpeng Wang, Ping He, Erjun Wei, Qiang Wang, Xudong Tang, Yiling Zhang, Feng Zhu, Zhongyuan Shen
Summary: This study successfully cloned genes related to protein transport in Nosema bombycis and found interactions between the subunits of these genes. Silencing key genes in Nosema bombycis using RNA interference can inhibit its proliferation.
MICROBIOLOGY SPECTRUM
(2022)
Article
Plant Sciences
Masayuki Ishikawa, Tetsuya Yoshida, Momoko Matsuyama, Yusuke Kouzai, Akihito Kano, Kazuhiro Ishibashi
Summary: In this study, targeted mutagenesis of four susceptible genes in tomato using CRISPR/Cas9 technology conferred strong resistance against the emerging virus ToBRFV. The quadruple-mutant plants showed highly durable resistance and also exhibited resistance to other tobamovirus species. This provides a valuable genetic resource for future disease-resistant tomato breeding.
Review
Multidisciplinary Sciences
Muneer Ahmed Khoso, Hailong Zhang, Mir Hassan Khoso, Tika Ram Poudel, Sindho Wagan, Tamar Papiashvili, Sudipta Saha, Abid Ali, Ghulam Murtaza, Hakim Manghwar, Fen Liu
Summary: The cytoskeleton is a crucial component in eukaryotic organisms, particularly in plant cells, playing a critical role in cellular mechanisms such as locomotion and morphological transformations. Comprised of microtubules, microfilaments, and intermediate filaments, the cytoskeleton regulates cell wall formation and remodeling. Recent studies highlight its importance in intracellular transport processes, and the flaws in its organization lead to abnormal organogenesis observed in plant mutants. Protein compartmentalization and abundance within cells are predominantly regulated by vesicle/membrane transport, involving various proteins. This review presents the latest research on the plant cytoskeleton and its role in vesicle transport, predicting future research directions and providing guidance for further investigation.
Article
Multidisciplinary Sciences
Timothy J. Hawkins, Michaela Kopischke, Patrick J. Duckney, Katarzyna Rybak, David A. Mentlak, Johan T. M. Kroon, Mai Thu Bui, A. Christine Richardson, Mary Casey, Agnieszka Alexander, Geert De Jaeger, Monika Kalde, Ian Moore, Yasin Dagdas, Patrick J. Hussey, Silke Robatzek
Summary: This study reveals that NET4A and NET4B members of the NET family play an essential role in the reorganization of guard cell actin during stomatal closure, mediating the molecular link between actin filaments and the tonoplast through interaction with RABG3b.
NATURE COMMUNICATIONS
(2023)
Article
Plant Sciences
Vendula Pukysova, Adria Sans Sanchez, Jiri Rudolf, Tomasz Nodzynski, Marta Zwiewka
Summary: To compensate for their sessile lifestyle, plants have developed adaptations at the level of subcellular trafficking to maintain cellular homeostasis and membrane integrity under osmotic stress conditions. The activity of flippases, such as P4-ATPases, is crucial for the formation of vesicles, which are involved in processes like endocytosis and secretion. Lack of flippase activity leads to defects in membrane and Golgi network, resulting in impaired vesicle formation and movement, and decreased intracellular trafficking flexibility, thereby affecting plant adaptation to osmotic stress.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Microbiology
Julianna Han, Ketaki Ganti, Veeresh Kumar Sali, Carly Twigg, Yifeng Zhang, Senthamizharasi Manivasagam, Chieh-Yu Liang, Olivia A. Vogel, Iris Huang, Shanan N. Emmanuel, Jesse Plung, Lillianna Radoshevich, Jasmine T. Perez, Anice C. Lowen, Balaji Manicassamy
Summary: It is well documented that influenza A viruses selectively package 8 distinct viral ribonucleoprotein complexes (vRNPs) into each virion, but the exact role of host factors in genome assembly is not fully understood. This study shows that Rab11a+ vesicles play a crucial role in the congregation and assembly of vRNPs, enabling specific genome assembly and production of infectious virus particles. However, in the absence of Rab11a, viral RNA segments fail to congregate properly, resulting in defects in virus assembly and increased production of non-infectious particles.
Article
Plant Sciences
Qingchen Rui, Xiaoyun Tan, Feng Liu, Yanbin Li, Xin Liu, Bingxuan Li, Junxia Wang, Huiting Yang, Lixin Qiao, Tingting Li, Shuo Fang, Rongrong Gao, Wang Wang, Sebastian Y. Bednarek, Yiqun Bao
Summary: SYP31 and SYP32 play roles in modulating Golgi morphology and pollen development by regulating protein trafficking and Golgi structure, showing partially redundant functions in pollen development.
Article
Plant Sciences
Madhumitha Narasimhan, Michelle Gallei, Shutang Tan, Alexander Johnson, Inge Verstraeten, Lanxin Li, Lesia Rodriguez, Huibin Han, Ellie Himschoot, Ren Wang, Steffen Vanneste, Judit Sanchez-Simarro, Fernando Aniento, Maciek Adamowski, Jiri Friml
Summary: This study reexamined the effects of auxin on PIN endocytic trafficking and found that high concentrations of NAA and IAA have nonspecific effects on the endomembrane system, while low concentrations specifically promoted endocytosis of PIN2. This contributes to polarity maintenance and highlights the nonspecific effects of high auxin levels on trafficking and endomembrane compartments.
Article
Nanoscience & Nanotechnology
Qingxia Wang, Yi Xiao, Jiarui Zhu, Luona Ye, Longyu Zhang, Lei Wang, Xuxia Wang, Huimin Pang, Jing Li, Sheng Yuan, Li Niu, Miao Chen, Yunjun Yan, Li Xu, Jinyong Yan
Summary: The genetically programmable lipase nanoreactor demonstrated improved stability under various harsh conditions and achieved an 86% yield in fatty acid methyl ester synthesis at a high concentration of waste cooking oil (200 mM), showcasing its robustness and feasibility in biodiesel production.
ACS APPLIED BIO MATERIALS
(2021)
Article
Cell Biology
William Kasberg, Peter Luong, Michael G. Hanna, Kayla Minushkin, Annabelle Tsao, Raakhee Shankar, Samuel Block, Anjon Audhya
Summary: Coat protein complex II (COPII) is crucial for the packaging of secretory cargoes in membrane-enclosed transport carriers. This study demonstrates that anterograde cargo transport from the endoplasmic reticulum (ER) can still occur even when the Sar1 protein is depleted, although with reduced efficiency. The findings highlight alternative mechanisms by which COPII promotes transport carrier biogenesis.
Article
Plant Sciences
Ryan C. Eng, Laryssa S. Halat, Samuel J. Livingston, Tatsuya Sakai, Hiroyasu Motose, Geoffrey O. Wasteneys
PLANT AND CELL PHYSIOLOGY
(2017)
Article
Plant Sciences
Marketa Absolonova, Mary J. Beilby, Aniela Sommer, Marion C. Hoepflinger, Ilse Foissner
Review
Cell Biology
Andrei Smertenko, Farhah Assaad, Frantisek Baluska, Magdalena Bezanilla, Henrik Buschmann, Georgia Drakakaki, Marie-Theres Hauser, Marcel Janson, Yoshinobu Mineyuki, Ian Moore, Sabine Mueller, Takashi Murata, Marisa S. Otegui, Emmanuel Panteris, Carolyn Rasmussen, Anne-Catherine Schmit, Jozef Samaj, Lacey Samuels, L. Andrew Staehelin, Daniel Van Damme, Geoffrey Wasteneys, Viktor Zarsky
TRENDS IN CELL BIOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Yuan Ruan, Laryssa S. Halat, Deirdre Khan, Sylwia Jancowski, Chris Ambrose, Mark F. Belmonte, Geoffrey O. Wasteneys
Article
Multidisciplinary Sciences
Heidi Pertl-Obermeyer, Peter Lackner, Waltraud X. Schulze, Marion C. Hoepflinger, Margit Hoeftberger, Ilse Foissner, Gerhard Obermeyer
Article
Plant Sciences
Zhongshou Wu, Meng Li, Oliver Xiaoou Dong, Shitou Xia, Wanwan Liang, Yongkang Bao, Geoffrey Wasteneys, Xin Li
Article
Plant Sciences
Marketa Absolonova, Ilse Foissner, Aniela Sommer
Article
Developmental Biology
Arun Sampathkumar, Alexis Peaucelle, Miki Fujita, Christoph Schuster, Staffan Persson, Geoffrey O. Wasteneys, Elliot M. Meyerowitz
Article
Plant Sciences
Alexey Eremin, Alexander A. Bulychev, Christopher Kluge, Jeremy Harbinson, Ilse Foissner
Article
Plant Sciences
Patrick T. Martone, Kyra Janot, Miki Fujita, Geoffrey Wasteneys, Katia Ruel, Jean-Paul Joseleau, Jose M. Estevez
Editorial Material
Biochemistry & Molecular Biology
Geoffrey O. Wasteneys
Article
Plant Sciences
Laryssa Halat, Katherine Gyte, Geoffrey Wasteneys
Review
Plant Sciences
Laryssa Sophia Halat, Breanne Bali, Geoffrey Wasteneys
Summary: The transition from cell division to differentiation in primary roots is influenced by gradients of phytohormones and the reorganization of microtubules. The microtubule-associated protein CLASP acts as an important intermediate between hormone signaling and microtubule organization. New tools and methods, such as hormone sensors and computer modeling, allow researchers to accurately visualize the belowground growth dynamics of plants.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Alexander A. Bulychev, Ilse Foissner
PLANT SIGNALING & BEHAVIOR
(2017)
Article
Plant Sciences
Marcus Woodley, Adam Mulvihill, Miki Fujita, Geoffrey O. Wasteneys